Agilent Technologies 2100 Bioanalyzer System Mode d'emploi

Agilent Technologies

Agilent 2100 Bioanalyzer

System

Maintenance and Troubleshooting

Guide

Maintenance and Troubleshooting

Warranty

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receive no greater than Restricted Rights as

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applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

Notices

2017

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G2946-90003 Rev. B

Edition

02/2017

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

For Research Use Only

Not for use in diagnostic procedures

Maintenance and Troubleshooting 3

In this Book

This manual provides maintenance and troubleshooting information for the

Agilent 2100 Bioanalyzer system. It includes essential measurement practices,

troubleshooting hints for hardware, software and applications, maintenance

procedures and a list of spare parts and accessories.

This manual is based on the 2100 Expert Software revision B.02.08. Other

software revisions may have an impact on results.

4 Maintenance and Troubleshooting

Contents

1 Essential Measurement Practices 7

Overview 8

Tools and Handling 9

Chip Priming Station 10

Reagents and Reagent Mixes 11

Samples 12

Chips 13

Agilent 2100 Bioanalyzer System 14

2 Troubleshooting the Instrument Communication 15

Verify the Instrument Communication 16

Troubleshooting Communication Issues 18

Changing COM Port Settings 20

USB to Serial Adapter 21

3 Troubleshooting the 2100 Expert Software 25

Run Installation Qualification Test 26

4 Hardware Diagnostics 29

Overview 30

Diagnostic Test Procedure 32

5 Troubleshooting the DNA Application 35

Overview 36

Symptoms (DNA) 37

Symptoms (High Sensitivity DNA) 62

6 Troubleshooting the RNA Application 67

Overview 68

Symptoms (RNA) 69

Maintenance and Troubleshooting 5

Contents

7 Troubleshooting the Protein Application 87

Overview 88

Symptoms (Protein) 89

Symptoms (High Sensitivity Protein) 112

8 Maintenance of the Electrode Cartridge 115

Overview 116

DNA and Protein Assays 117

RNA Nano Assay 119

RNA Pico or Small RNA Assay 123

How to Clean the Pin Set of the Electrode Cartridge 126

9 Maintenance of the Chip Priming Station 131

Overview 132

Replacing the Syringe 133

Cleaning the Syringe Adapter 134

Replacing the Syringe Adapter 136

Replacing the Gasket 137

Checking the Chip Priming Station for Proper Performance - Seal Test 139

10 Maintenance of the Agilent 2100 Bioanalyzer instrument 141

Overview 142

Cleaning the Lens 143

Changing the Fuses 144

11 Maintenance of the Vortexer 149

Changing the Adapter 150

12 Spare Parts and Accessories 151

Overview 152

6 Maintenance and Troubleshooting

Contents

Maintenance and Troubleshooting

Agilent Technologies

Essential Measurement Practices

Overview 8

Tools and Handling 9

Chip Priming Station 10

Reagents and Reagent Mixes 11

Gel and Gel-Dye Mix 11

Samples 12

Chips 13

Agilent 2100 Bioanalyzer System 14

8 Maintenance and Troubleshooting

Essential Measurement Practices

Overview

This section lists all user relevant hints on handling tools, chips, reagents and

the Agilent 2100 Bioanalyzer system. For the latest information on

assay-related hints, go to the Agilent web site at:

www.agilent.com/genomics/bioanalyzer

Maintenance and Troubleshooting 9

Essential Measurement Practices

Tools and Handling

•Always follow the GLP-rules established in the laboratory.

•Always wear gloves when handling chips to prevent contamination.

•When pipetting sample, use non-filter pipette tips that are of adequate size.

Pipette tips that are too large will lead to poor quantitation accuracy.

•Change pipette tips between steps to avoid cross-contamination.

•Always insert the pipette tip to the bottom of the well when dispensing

liquid. Placing the tip at the edge of the well leads to bubbles and poor

results. Holding the pipette at a slight angle will ensure proper dispensing

of the liquid.

•Use a new syringe and electrode cleaner with each new kit.

10 Maintenance and Troubleshooting

Essential Measurement Practices

Chip Priming Station

•Refer to the appropriate Kit Guide for the correct position of the syringe

clip and base plate.

•Replace the syringe with each new kit.

•Check the performance of the chip priming station by applying the seal test

on a monthly basis. For details see “Maintenance of the Chip Priming

Station” on page 131. If necessary, replace the gasket and/or adapter.

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