Leica M165 FC Manuel utilisateur

Leica M165 FC

Leica M205 FA / M205 FCA

User Manual

Leica M Series User Manual 2

General Instructions

Safety concept

Before using your microscope for the ﬁrst

time, please read the "Safety concept" book-

let included with your instrument. It contains

additional information about handling and

care.

Use in clean rooms

The Leica M series can be used in clean rooms

without any problems.

Cleaning

ODo not use any unsuitable cleaning agents,

chemicals or techniques for cleaning.

ONever use chemicals to clean colored

surfaces or accessories with rubberized

parts. This could damage the surfaces,

and specimens could be contaminated by

abraded particles.

OIn most cases, we can provide special solu-

tions on request. Some products can be

modiﬁed, and we can oﬀer other accesso-

ries for use in clean rooms.

Servicing

ORepairs may only be carried out by Leica

Microsystems-trained service technicians.

Only original Leica Microsystems spare

parts may be used.

Responsibilities of person in charge of

instrument

OEnsure that the Leica stereo microscope

is operated, maintained and repaired by

authorized and trained personnel only.

Leica M Series User Manual 3

Important Safety Notes

User manual

The individual modules of the Leica M stereo

microscopy series include an interactive

CD-ROM with all relevant user manuals in

20 other languages. Keep it in a safe place,

and readily accessible to the user. User manu-

als and updates are also available for you

to download and print from our website at

www.stereomicroscopy.com.

This User Manual describes the special func-

tions of the individual modules of the Leica M

stereo microscopy series and contains impor-

tant instructions for their operational safety,

maintenance, and accessories.

The "Safety concept" booklet contains addi-

tional safety information regarding the service

work, requirements and the handling of the

stereo microscope, accessories and electrical

accessories as well as general safety instruc-

tions.

You can combine individual system articles

with articles from external suppliers (e.g. cold

light sources, etc.). Please read the user manual

and the safety requirements of the supplier.

Before installing, operating or using the instru-

ments, read the user manuals listed above.

In particular, please observe all safety instruc-

tions.

To maintain the unit in its original condition

and to ensure safe operation, the user must

follow the instructions and warnings contained

in these user manuals.

Special manuals

Special manuals are provided for a number of

accessories:

OUser Manual M2-216-1 for lamp housing

with high-pressure mercury burner.

OUser Manual for ebq 100 isolated supply

unit for 100 W high-pressure mercury

burners.

OUser Manual M2-267-1 for the motorized

focus system.

OUser Manual M2-166-2 for FluoCombi III.

Leica M Series User Manual 4

Symbols Used

Warning of a danger

•This symbol indicates especially impor-

tant information that must be read and

complied with.

Failure to comply can cause the following:

OHazards to personnel

OFunctional disturbances or damaged

instruments

Warning of hazardous electrical voltage

This symbol indicates especially impor-

tant information that is mandatory to

read and observe.

Failure to comply can cause the following:

OHazards to personnel

OFunctional disturbances or damaged

instruments

Danger due to hot surface

This symbol warns against touching

accessible hot surfaces, e.g. those of

light bulbs.

Important information

This symbol indicates additional infor-

mation or explanations that intend to

provide clarity.

Explanatory notes

OThis symbol within the text stands for addi-

tional information and explanations.

Figures

(1) Numbers in parentheses within the

descriptions relate to the ﬁgures and the

items within those ﬁgures.

Leica M Series User Manual 5

Safety Instructions

Description

The individual modules fulﬁll the highest

requirements for observation and documenta-

tion of Leica stereo microscopes of the Mseries.

Intended use

ORefer to "Safety Concept" booklet

Non-intended use

ORefer to "Safety Concept" booklet

Never use M series microscopes or their compo-

nents for surgical procedures (e.g. on the eye)

unless they are speciﬁcally intended for that

purpose.

The instruments and accessories described in

this operating manual have been tested for

safety and potential hazards. The responsible

Leica aﬃliate must be consulted whenever

the instrument is altered, modiﬁed or used in

conjunction with non-Leica components that

are outside of the scope of this manual.

Unauthorized alterations to the instrument or

noncompliant use shall void all rights to any

warranty claims.

Place of use

ORefer to "Safety Concept" booklet

OElectrical components must be placed at

least 10 cm away from the wall and from

ﬂammable substances.

OAvoid large temperature ﬂuctuations,

direct sunlight and vibrations. These condi-

tions can distort measurements and micro-

graphic images.

OIn warm and warm-damp climatic zones,

the individual components require special

care in order to prevent the build-up of

fungus.

Responsibilities of person in charge of

instrument

ORefer to "Safety Concept" booklet

Ensure that:

OThe M series stereo microscopes and

accessories are operated, maintained and

repaired by authorized and trained person-

nel only.

OAll operators have read, understood and

observe this User Manual, and particularly

the safety instructions.

Leica M Series User Manual 6

Safety Instructions (Continued)

Repairs, service work

ORefer to "Safety Concept" booklet

OOnly original Leica Microsystems spare

parts may be used.

OBefore opening the instruments, switch oﬀ

the power and unplug the power cable.

Touching the live circuit can cause

injury.

Transport

OUse the original packaging for shipping or

transporting the individual modules of the

Leica M stereo microscopy series and the

accessory components.

OIn order to prevent damage from vibrations,

disassemble all moving parts that (accord-

ing to the user manual) can be assembled

and disassembled by the customer and

pack them separately.

Integration in third-party products

ORefer to "Safety Concept" booklet

Disposal

ORefer to "Safety Concept" booklet

Legal requirements

ORefer to "Safety Concept" booklet

EC Declaration of Conformity

ORefer to "Safety Concept" booklet

Health risks

•Workplaces with stereo microscopes

facilitate and improve the viewing task,

but they also impose high demands on the eyes

and holding muscles of the user. Depending on

the duration of uninterrupted work, asthenopia

and musculoskeletal problems may occur. For

this reason, appropriate measures for reduction

of the workload must be taken:

OOptimal arrangement of workplace, work

assignments and work ﬂow (changing tasks

frequently).

OThorough training of the personnel, giving

consideration to ergonomic and organiza-

tional aspects.

OThe ergonomic design and construction of

the Leica M stereo microscopy series are

intended to reduce the exertion of the user

to a minimum.

Leica M Series User Manual 7

Safety Instructions (Continued)

•Direct contact with eyepieces is a

potential transmission method for

bacterial and viral infections of the eye.

The risk can be kept to a minimum by using

personal eyepieces for each individual or

detachable eyecups.

Light sources: Safety regulations

Protective measures of the manufacturer:

OUV protection screen in front of the speci-

men plane prevents the user from looking

directly into the UV rays.

ODummy ﬁlter carriers in the free positions

of the rapid ﬁlter changer prevent direct UV

radiation from reaching the eyes.

OUV ﬁlters are installed in the observation

beam paths to protect the eyes.

OThe stray-light protection on the lamp

housing prevents irradiation of the hands.

Warning

•UV radiation could damage the eyes.

Therefore:

ONever look into the light spot on the speci-

men plane without a UV protection screen.

ONever look into the eyepieces if no excita-

tion ﬁlter is in the beam path.

OFill empty ﬁlter positions with dummy ﬁlter

carriers (e.g. M165 FC).

ODo not select a white, strongly reﬂective

background for the specimen.

Leica M Series User Manual 8

Safety Instructions (Continued)

Supply unit

Always unplug the supply unit from the power

supply:

OWhen installing and disassembling the

lamp housing

OBefore opening the lamp housing

OWhen replacing the high-pressure mercury

lamp and other parts, such as the heat-

absorbing ﬁlter or the collector

ODuring maintenance work on the supply

unit

Lamp housing

•Never open the lamp housing while the

lamp is switched on. Risk of explosion,

UV exposure, blinding!

•Before opening the lamp housing, allow

it to cool oﬀ for at least 15 minutes.

Danger of explosion!

•Never cover the air duct on the lamp

housing. Danger of ﬁre!

Mercury lamp

OFollow the user manual and safety instruc-

tions provided by the lamp manufacturer,

and, in particular, the section on how to

proceed if a lamp breaks and releases

mercury.

OFor transport, remove the mercury lamp,

transport it in its original packaging and

protect moving parts in the lamp housing

using the transport anchors.

OWhen it has reached the end of its rated life

(follow the manufacturer's speciﬁcations

and the minute meter on the supply unit).

OTo minimize the risk of explosion, replace a

discolored mercury lamp promptly.

OLeica Microsystems assumes no liability

for damage caused by exploding, incor-

rectly installed or improperly used mercury

lamps.

Leica M Series User Manual 9

Contents

General Instructions 2

Important Safety Notes 3

Symbols Used 4

Safety Instructions 5

The Leica M Series for Fluorescence

Congratulations! 13

A Step Towards Inﬁnity 14

The Electronics: Comfort, Convenience and Safety for Your Experiments15

The Modular Design: Everything is Relative 16

Maximum Compatibility 17

On We Go 18

Assembly

Assembling the Focusing Column for TL Bases 20

Assembling the Focusing Column With an Incident-light Base 21

Motorized Focus: Restricting the Travel Path 22

Optics Carrier 23

Tube 24

Eyepieces 25

Objective 26

UV Protection Screen: Assembly 27

Objective Nosepiece – Assembly 28

Objective Nosepiece – Adjusting Parfocality 29

Leica FluoCombi III – Preparations 30

Leica FluoCombi III – Assembly 31

Leica FluoCombi III – Adjusting the Parfocality 33

Leica FluoCombi III – Centering the Objectives 35

Leica FluoCombi III – Filter and UV Protection Screen 36

Transmitted-light Base Leica TL BFDF: Before First Use 37

Transmitted-light Base Leica TL BFDF 38

Leica TL RC / Leica TL RCI 39

Leica IsoPro Manual Mechanical Stage: Assembly 40

Leica IsoPro Motorized Mechanical Stage: Assembly 43

Cables: Connections 47

Cables: Cable Duct 48

Cables: Diagram 49

Leica LED5000 MCI 50

Leica LED5000 MCI: Alternative Assembly 51

Leica LED5000 RL: Assembly 52

Leica EL6000 – Safety Notes 54

Leica EL6000 – Preparations 57

Leica EL6000 – Connection to the Fluorescence Lamp Housing 60

Leica EL6000 – Replacing the Lamp 62

Leica EL6000 – Using the Internal Shutter 63

Leica M Series User Manual 10

Quick Start Guide

The Fastest Route to Success 65

Overview of an M Series Microscope 66

The Correct Interpupillary Distance 67

Using the Eyepieces 68

Focusing 69

Adjusting the Resistance of the Focus Drive 70

Changing Magniﬁcation (Zoom) 71

Ratchet Steps and Magniﬁcation Levels 72

Parfocality: More Comfort and Convenience for Your Work 73

Iris Diaphragm 74

Eyepieces

Magniﬁcation Factors of the Eyepieces 76

Health Notes 77

Dioptric Correction 78

Dioptric Correction and Parfocality 79

Graticules 80

Photography & Video

Photography & Video 82

Photo Tubes and C-mounts 83

Trinocular Video/Phototube 50 % 84

Trinocular Video/Phototube 100 % 85

Trinocular Video/Phototube 100 %: ErgoTube 5°– 45° 86

Microscope Carrier

The Objective Nosepiece 88

Objectives and Optical Accessories

The Diﬀerent Types of Objectives 90

Bases

Leica TL ST Transmitted-light Base: Controls 92

Leica TL ST Transmitted-light Base: Operation 93

Leica TL ST Transmitted-light Base: Changing Bulbs 94

Leica TL BFDF Transmitted-light Base: Controls 95

Leica TL BFDF Transmitted-light Base: Operation 96

Leica TL RC / Leica TL RCI: Controls 97

Leica TL RCI: The Deﬂection Mirror 98

Leica TL RCI: Color Intensity and Temperature 99

Leica TL RC / Leica TL RCI: Operation 100

Leica TL RCI: Methods in Transmitted Light 101

Leica TL RCI: Relief Images 102

Using Filters 104

Leica IsoPro (Non-motorized): Controls 105

Leica IsoPro (Motorized): Controls 106

Contents (Continued)

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