Leica M125 C Manuel utilisateur

Leica M125 C

Leica M165 C

Leica M205 C

Leica M205 A

User Manual

Leica M Series User Manual 2

General Instructions

Safety concept

Before using your microscope for the ﬁrst

time, please read the "Safety concept" booklet

included with your instrument. It contains addi-

tional information about handling and care.

Use in clean rooms

The Leica M series can be used in clean rooms

without any problems.

Cleaning

ODo not use any unsuitable cleaning agents,

chemicals or techniques for cleaning.

ONever use chemicals to clean colored

surfaces or accessories with rubberized

parts. This could damage the surfaces,

and specimens could be contaminated by

abraded particles.

OIn most cases, we can provide special solu-

tions on request. Some products can be

modiﬁed, and we can oﬀer other accesso-

ries for use in clean rooms.

Servicing

ORepairs may only be carried out by Leica

Microsystems-trained service technicians.

Only original Leica Microsystems spare

parts may be used.

Responsibilities of person in charge of

instrument

OEnsure that the Leica stereo microscope

is operated, maintained and repaired by

authorized and trained personnel only.

Leica M Series User Manual 3

Safety Concept

The individual modules of the Leica M stereo

microscopy series include an interactive

CD-ROM with all relevant user manuals in 20

other languages. Keep it in a safe place, and

readily accessible to the user. User manu-

als and updates are also available for you

to download and print from our web site at

www.stereomicroscopy.com.

This operating manual describes the special

functions of the individual modules of the Leica

M stereo microscopy series and contains impor-

tant instructions for their operational safety,

maintenance, and accessories.

The "Safety concept" booklet contains addi-

tional safety information regarding the service

work, requirements and the handling of stereo

microscope, accessories and electrical accesso-

ries as well as general safety instructions.

You can combine individual system articles

with articles from external suppliers (e.g. cold

light sources, etc.). Please read the user manual

and the safety requirements of the supplier.

Before installing, operating or using the instru-

ments, read the user manuals listed above. In

particular, please observe all safety instructions.

To maintain the unit in its original condition

and to ensure safe operation, the user must

follow the instructions and warnings contained

in these user manuals.

Leica M Series User Manual 4

Symbols Used

Warning of a danger

This symbol indicates especially impor-

tant information that must be read and

complied with. Failure to comply can cause the

following:

OHazards to personnel

OFunctional disturbances or damaged

instruments

Warning of hazardous electrical voltage

This symbol indicates especially impor-

tant information that, if not observed,

can cause the following:

OHazards to personnel

OFunctional disturbances or damaged

instruments

Danger due to hot surface

This symbol warns against touching hot

surfaces, e.g. those of light bulbs.

Important information

This symbol indicates additional infor-

mation or explanations that intend to

provide clarity.

Action

OThis symbol refers to actions described in

the text that are to be carried out.

Figures

(1) Numbers in brackets within the descrip-

tions relate to the ﬁgures and the items

within those ﬁgures.

Leica M Series User Manual 5

Safety Instructions

Description

The individual modules fulﬁll the highest

requirements for observation and documenta-

tion of Leica stereo microscopes of the Mseries.

Intended use

ORefer to "Safety Concept" booklet

Non-intended use

ORefer to "Safety Concept" booklet

Never use M series microscopes or their compo-

nents for surgical procedures (e.g. on the eye)

unless they are speciﬁcally intended for that

purpose.

The instruments and accessories described in

this operating manual have been tested for

safety and potential hazards. The responsible

Leica aﬃliate must be consulted whenever

the instrument is altered, modiﬁed or used in

conjunction with non-Leica components that

are outside of the scope of this manual.

Unauthorized alterations to the instrument or

noncompliant use shall void all rights to any

warranty claims.

Place of use

ORefer to "Safety Concept" booklet

OElectrical components must be placed at

least 10 cm away from the wall and from

ﬂammable substances.

OAvoid large temperature ﬂuctuations,

direct sunlight and vibrations. These condi-

tions can distort measurements and micro-

graphic images.

OIn warm and warm-damp climatic zones,

the individual components require special

care in order to prevent the build-up of

fungus.

Responsibilities of person in charge of

instrument

ORefer to "Safety Concept" booklet

Ensure that:

OThe M series stereo microscopes and

accessories are operated, maintained and

repaired by authorized and trained person-

nel only.

OAll operators have read, understood and

observe this User Manual, and particularly

the safety instructions.

Leica M Series User Manual 6

Safety Instructions (Continued)

Repairs, service work

ORefer to "Safety Concept" booklet

OOnly original Leica Microsystems spare

parts may be used.

OBefore opening the instruments, switch oﬀ

the power and unplug the power cable.

Touching the live circuit can cause

injury.

Transport

OUse the original packaging for shipping or

transporting the individual modules of the

Leica M stereo microscopy series and the

accessory components.

OIn order to prevent damage from vibrations,

disassemble all moving parts that (accord-

ing to the user manual) can be assembled

and disassembled by the customer and

pack them separately.

Integration in third-party products

ORefer to "Safety Concept" booklet

Disposal

ORefer to "Safety Concept" booklet

Legal requirements

ORefer to "Safety Concept" booklet

EC Declaration of Conformity

ORefer to "Safety Concept" booklet

Health risks

Workplaces with stereo microscopes

facilitate and improve the viewing task,

but they also impose high demands on the eyes

and holding muscles of the user. Depending on

the duration of uninterrupted work, asthenopia

and musculoskeletal problems may occur. For

this reason, appropriate measures for reduction

of the workload must be taken:

OOptimal arrangement of workplace, work

assignments and work ﬂow (changing tasks

frequently).

OThorough training of the personnel, giving

consideration to ergonomic and organiza-

tional aspects.

OThe ergonomic design and construction of

the Leica M stereo microscopy series are

intended to reduce the exertion of the user

to a minimum.

Leica M Series User Manual 7

Safety Instructions (Continued)

Direct contact with eyepieces is a

potential transmission method for

bacterial and viral infections of the eye.

The risk can be kept to a minimum by using

personal eyepieces for each individual or

detachable eyecups.

Light sources: Safety regulations

Protective measures of the manufacturer:

OUV protection screen in front of the speci-

men plane prevents the user from looking

directly into the UV rays.

ODummy ﬁlter carriers in the free positions

of the rapid ﬁlter changer prevent direct UV

radiation from reaching the eyes.

OUV ﬁlters are installed in the observation

beam paths to protect the eyes.

OThe stray-light protection on the lamp

housing prevents irradiation of the hands.

Warning

UV radiation could damage the eyes.

Therefore:

ONever look into the light spot on the speci-

men plane without a UV protection screen.

ONever look into the eyepieces if no excita-

tion ﬁlter is in the beam path.

ODo not select a white, strongly reﬂective

background for the specimen.

Leica M Series User Manual 8

Safety Instructions (Continued)

Supply unit

Always unplug the supply unit from the power

supply:

OWhen installing and disassembling the

lamp housing

OBefore opening the lamp housing

OWhen replacing the high-pressure mercury

lamp and other parts, such as the heat-

absorbing ﬁlter or the collector

ODuring maintenance work on the supply

unit

Lamp housing

Never open the lamp housing while the

lamp is switched on. Risk of explosion,

UV exposure, blinding!

Before opening the lamp housing, allow

it to cool oﬀ for at least 15 minutes.

Danger of explosion!

Never cover the air duct on the lamp

housing. Danger of ﬁre!

Mercury lamp

OFollow the user manual and safety instruc-

tions provided by the lamp manufacturer,

and, in particular, the section on how to

proceed if a lamp breaks and releases

mercury.

OFor transport, remove the mercury lamp,

transport it in its original packaging and

protect moving parts in the lamp housing

using the transport anchors.

OWhen it has reached the end of its rated life

(follow the manufacturer's speciﬁcations

and the minute meter on the supply unit).

OTo minimize the risk of explosion, replace a

discolored mercury lamp promptly.

OLeica Microsystems assumes no liability

for damage caused by exploding, incor-

rectly installed or improperly used mercury

lamps.

Leica M Series User Manual 9

Contents

Transmitted-light Base Leica TL BFDF: Before First Use 30

Transmitted-light Base Leica TL BFDF 31

Leica TL RC / Leica TL RCI 32

Leica IsoPro Manual Cross-stage: Assembly 33

Leica IsoPro Motorized Cross-stage: Assembly 36

Cables: Terminals 40

Cables: Cable Duct 41

Cables: Diagram 42

Leica LED5000 MCI 43

Leica LED5000 MCI: Alternative Assembly 44

Leica LED5000 RL: Assembly 45

Leica LED5000 RL: Installing Optional Accessories 47

Leica LED5000 RL: Use 48

Leica LED5000 NVI: Assembly on High-Performance Stereo

Microscopes (Leica M125 C / Leica M165 C / Leica M205 C) 50

Leica LED5000 NVI: Installing Optional Accessories 53

Quick Start Guide

The Fastest Route to Success 56

Overview of an M Series Microscope 57

The Correct Interpupillary Distance 58

Using the Eyepieces 59

Focusing 60

Adjusting the Resistance of the Focus Drive 61

General Instructions 2

Safety Concept 3

Symbols Used 4

Safety Instructions 5

The Leica M Series

Congratulations! 13

A Step Towards Inﬁnity 14

The Electronics: Comfort, Convenience and Safety for Your Experiments15

The Modular Design: Everything is Relative 16

Maximum Compatibility 17

On We Go 18

Assembly

Base and Focusing Column 20

Optics Carrier 21

Tube 22

Eyepieces 23

Objective 24

Objective Nosepiece – Assembly 25

Objective Nosepiece – Adjusting Parfocality 26

AX Carrier – Preparation 27

AX Carrier – Assembly 28

Transmitted-light Base Leica TL ST 29

Leica M Series User Manual 10

Changing the Magniﬁcation (Zoom) 62

Ratchet Steps and Magniﬁcation Levels 63

Parfocality: More Comfort and Convenience for Your Work 64

Iris Diaphragm 65

Eyepieces

Magniﬁcation Factors of the Eyepieces 67

Health Notes 68

Dioptric Correction 69

Dioptric Correction and Parfocality 70

Graticules 71

Photography & Video

Photography & Video 73

Photo Tubes and C-mounts 74

Trinocular Video/Phototube 50 % 75

Trinocular Video/Phototube 100 % 76

Microscope Carrier

The Microscope Carrier AX 78

The Objective Nosepiece 80

Objectives and Optical Accessories

The Diﬀerent Types of Objectives 82

Bases

Transmitted-light Base Leica TL ST: Controls 84

Transmitted-light Base Leica TL ST: Operation 85

Transmitted-light Base Leica TL ST: Lamp Replacement 86

Transmitted-light Base Leica TL BFDF: Controls 87

Transmitted-light Base Leica TL BFDF: Operation 88

Leica TL RC / Leica TL RCI: Controls 89

Leica TL RCI: The Deﬂection Mirror 90

Leica TL RCI: Color Intensity and Temperature 91

Leica TL RC / Leica TL RCI: Operation 92

Leica TL RCI: Methods in Transmitted Light 93

Leica TL RCI: Relief Images 94

Using Filters 96

Leica IsoPro (Non-motorized): Controls 97

Leica IsoPro (Motorized): Controls 98

Accessories

Leica Hand Wheel and Foot Switch 100

Leica SmartTouch 101

Leica PSC Controller 102

Contents (Continued)

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