Zeiss LSM 880 Manuel utilisateur

ZEISS LSM 880 INVERTED (i880) QUICK START GUIDE

The Zeiss LSM880 is a laser scanning confocal microscope with full confocal and multiphoton capability. The

confocal detectors are spectrally resolved and can be used with either the standard (single photon) excitation

lines or the multiphoton excitation lines. The non-descanned (NDD) detectors can only be used with multiphoton

excitation.

INSTRUMENT OVERVIEW

AND USER RESOURCES

FULL TURN ON SEQUENCE (FIRST ONE ON)

1) If the computer is off, turn it on and let it boot.

2) Turn on the main switch.

3) Turn on the Systems/PC switch. (When

touchscreen has booted, adjust heating and

CO2 under Microscope->Inkubation if

applicable.)

4) Turn on the Components switch. Wait for the

RTC to boot (see under table).

5) Log in to the computer.

6) Start Zen and select Start System Button.

7) Turn on laser lines you will be using. (If you are

using multiphoton excitation, turn on both lines

on even if you are just using one. Note that the

Ar and multiphoton lines take a while to turn on.

You can follow the warm-up progress by

selecting the laser and the Laser Properties

below)

FULL TURN OFF SEQUENCE (LAST ONE ON)

9) Save your data and transfer it to the fileshare.

8) Remove your sample and clean off your

objective. (If using incubation, turn temp and

CO2 regulation off and close CO2 tank using

main gray valve only.)

7) Turn off lasers.

6) Exit Zen.

5) Log out of the computer.

4) Turn off the Components switch.

3) Turn off the Systems/PC switch.

2) Wait a few minutes for the Ar laser to cool.

Turn off the main switch.

1) Do not turn off computer.

PARTIAL TURN OFF SEQUENCE

(Someone else is scheduled after your time slot)

9) Save your data and transfer it to the fileshare.

8) Remove your sample and clean off your

objective. (If using incubation, turn temp and

CO2 regulation off and close CO2 tank using

gray handle only.)

7) Turn off lasers.

6) Exit Zen.

5) Log out of the computer.

PARTIAL TURN ON SEQUENCE

(Microscope already on)

5) Log in to the computer.

6) Start Zen and select Start System Button.

7) Turn on laser lines you will be using. (If you are

using multiphoton excitation, turn on both lines

on even if you are just using one. Note that the

Ar and multiphoton lines take a while to turn on.

You can follow the warm-up progress by

selecting the laser and the Laser Properties

below)

LASER LINES

STANDARD OBJECTIVE SPECIFICATIONS

Mag

Immersion

medium

CS thickness

(mm)

WD (mm)

Objective Type

Trans > 0.5

10X

0.3

Air

0.17

5.2

EC Plan-Neofluar

350-1200nm

20X

0.5

Air

0.17

2.0

EC Plan-Neofluar

350-1250nm

32X

0.85

Water

0-0.17

1.1

C-Achroplan

450-1300nm

40X

1.1

Water

0.14-0.19

0.62

LD C-Apochromat

350-1150nm

40X

1.4

Oil

0.17

0.13

Plan-Apochromat

380-1200nm

10X

0.45

Water

0.17

1.8

C-Apochromat

350-1200nm

63X

0.75

Air

0-1.5

1.7

(at 0.75 CS)

LD Plan-Neofluar

350-950nm

NDD DETECTOR FILTERS

*Do not use any laser lines <700nm with the NDD detectors!

Autres manuels Zeiss Microscope

Zeiss

Zeiss EM 10 A Manuel utilisateur

Zeiss

Zeiss Standard 25 ICS Manuel utilisateur

Zeiss

Zeiss opmi lumera 700 Guide d'installation

Zeiss

Zeiss Axiovert 200 Manuel utilisateur

Zeiss

Zeiss Axioscope 7 Manuel utilisateur

Zeiss

Zeiss SV8 Manuel utilisateur

Zeiss

Zeiss opmi lumera 700 Manuel utilisateur

Zeiss

Zeiss Axiovert 25 C Manuel utilisateur

Zeiss

Zeiss SIGMA Series Manuel utilisateur

Zeiss

Zeiss TELAVAL 31 Manuel utilisateur

Zeiss

Zeiss LSM800-2 Manuel utilisateur

Zeiss

Zeiss Axio Imager 2 Manuel utilisateur

Zeiss

Zeiss LSM 710 Manuel utilisateur

Zeiss

Zeiss Axio Zoom.V16 Manuel utilisateur

Zeiss

Zeiss EXTARO 300 Manuel utilisateur

Zeiss

Zeiss Axioskop 2 plus Manuel utilisateur

Zeiss

Zeiss IV FL Manuel utilisateur

Zeiss

Zeiss Stemi 305 Manuel utilisateur

Zeiss

Zeiss Primostar 1 Manuel utilisateur

Zeiss

Zeiss Standard Pol Manuel utilisateur

Zeiss

Zeiss Axio Imager Manuel utilisateur

Zeiss

Zeiss Axiolab Pol Manuel utilisateur

Zeiss

Zeiss 880 Guide d'installation

Zeiss

Zeiss LSM 510 Manuel utilisateur

Manuels Microscope populaires d'autres marques

Thermo Scientific

Thermo Scientific Nicolet iN10 Mode d’emploi

National

National DC3-163 Manuel utilisateur

KERN Optics

KERN Optics OPE-1 Manuel utilisateur

OPTIKA MICROSCOPES

OPTIKA MICROSCOPES B-150D Manuel utilisateur

Prior

Prior Lux POL Manuel utilisateur

Opitec

Opitec Smartscoop 115615 Manuel utilisateur

KERN

KERN OPO-1 Manuel utilisateur

Topcon

Topcon SP-1P Manuel utilisateur

Motic

Motic PA43 Manuel utilisateur

Leica

Leica DVM5000 Guide de l'utilisateur

MshOt

MshOt MZ62 Manuel utilisateur

KERN Optics

KERN Optics OZR-5 Manuel utilisateur

VWR

VWR VisiScope 384 Series Manuel utilisateur

Nikon

Nikon ECLIPSE E200 POL Manuel utilisateur

Leica

Leica DI C800 Manuel

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manuel utilisateur

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manuel utilisateur

Olympus

Olympus SZ61 Manuel utilisateur