Leica M125 C Manuel utilisateur

Leica M125 C

Leica M165 C

Leica M205 C

Leica M205 A

User Manual

Leica M Series User Manual 2

General Instructions

Safety concept

Before using your microscope for the ﬁrst

time, please read the "Safety concept" booklet

included with your instrument. It contains addi-

tional information about handling and care.

Use in clean rooms

The Leica M series can be used in clean rooms

without any problems.

Cleaning

ODo not use any unsuitable cleaning agents,

chemicals or techniques for cleaning.

ONever use chemicals to clean colored

surfaces or accessories with rubberized

parts. This could damage the surfaces,

and specimens could be contaminated by

abraded particles.

OIn most cases, we can provide special solu-

tions on request. Some products can be

modiﬁed, and we can oﬀer other accesso-

ries for use in clean rooms.

Servicing

ORepairs may only be carried out by Leica

Microsystems-trained service technicians.

Only original Leica Microsystems spare

parts may be used.

Responsibilities of person in charge of

instrument

OEnsure that the Leica stereo microscope

is operated, maintained and repaired by

authorized and trained personnel only.

Leica M Series User Manual 3

Safety Concept

The individual modules of the Leica M stereo

microscopy series include an interactive

CD-ROM with all relevant user manuals in 20

other languages. Keep it in a safe place, and

readily accessible to the user. User manu-

als and updates are also available for you

to download and print from our web site at

www.stereomicroscopy.com.

This operating manual describes the special

functions of the individual modules of the Leica

M stereo microscopy series and contains impor-

tant instructions for their operational safety,

maintenance, and accessories.

The "Safety concept" booklet contains addi-

tional safety information regarding the service

work, requirements and the handling of stereo

microscope, accessories and electrical accesso-

ries as well as general safety instructions.

You can combine individual system articles

with articles from external suppliers (e.g. cold

light sources, etc.). Please read the user manual

and the safety requirements of the supplier.

Before installing, operating or using the instru-

ments, read the user manuals listed above. In

particular, please observe all safety instructions.

To maintain the unit in its original condition

and to ensure safe operation, the user must

follow the instructions and warnings contained

in these user manuals.

Leica M Series User Manual 4

Symbols Used

Read and understand user manual

before using this device.

Warning of a danger

•This symbol indicates especially impor-

tant information that must be read and

complied with. Failure to comply can cause the

following:

OHazards to personnel

OFunctional disturbances or damaged

instruments

Warning of hazardous electrical voltage

This symbol indicates especially impor-

tant information that, if not observed,

can cause the following:

OHazards to personnel

OFunctional disturbances or damaged

instruments

Danger due to hot surface

This symbol warns against touching hot

surfaces, e.g. those of light bulbs.

Important information

This symbol indicates additional infor-

mation or explanations that intend to

provide clarity.

Action

OThis symbol refers to actions described in

the text that are to be carried out.

Figures

(1) Numbers in brackets within the descrip-

tions relate to the ﬁgures and the items

within those ﬁgures.

Leica M Series User Manual 5

Safety Instructions

Description

The individual modules fulﬁll the highest

requirements for observation and documenta-

tion of Leica stereo microscopes of the Mseries.

Intended use

ORefer to "Safety Concept" booklet

Non-intended use

ORefer to "Safety Concept" booklet

Never use M series microscopes or their compo-

nents for surgical procedures (e.g. on the eye)

unless they are speciﬁcally intended for that

purpose.

The instruments and accessories described in

this operating manual have been tested for

safety and potential hazards. The responsible

Leica aﬃliate must be consulted whenever

the instrument is altered, modiﬁed or used in

conjunction with non-Leica components that

are outside of the scope of this manual.

Unauthorized alterations to the instrument or

noncompliant use shall void all rights to any

warranty claims.

Place of use

ORefer to "Safety Concept" booklet

OElectrical components must be placed at

least 10 cm away from the wall and from

ﬂammable substances.

OAvoid large temperature ﬂuctuations,

direct sunlight and vibrations. These condi-

tions can distort measurements and micro-

graphic images.

OIn warm and warm-damp climatic zones,

the individual components require special

care in order to prevent the build-up of

fungus.

Responsibilities of person in charge of

instrument

ORefer to "Safety Concept" booklet

Ensure that:

OThe M series stereo microscopes and

accessories are operated, maintained and

repaired by authorized and trained person-

nel only.

OAll operators have read, understood and

observe this User Manual, and particularly

the safety instructions.

Leica M Series User Manual 6

Safety Instructions (Continued)

Repairs, service work

ORefer to "Safety Concept" booklet

OOnly original Leica Microsystems spare

parts may be used.

OBefore opening the instruments, switch oﬀ

the power and unplug the power cable.

Touching the live circuit can cause

injury.

Transport

OUse the original packaging for shipping or

transporting the individual modules of the

Leica M stereo microscopy series and the

accessory components.

OIn order to prevent damage from vibrations,

disassemble all moving parts that (accord-

ing to the user manual) can be assembled

and disassembled by the customer and

pack them separately.

Integration in third-party products

ORefer to "Safety Concept" booklet

Disposal

ORefer to "Safety Concept" booklet

Legal requirements

ORefer to "Safety Concept" booklet

EC Declaration of Conformity

ORefer to "Safety Concept" booklet

Health risks

•Workplaces with stereo microscopes

facilitate and improve the viewing task,

but they also impose high demands on the eyes

and holding muscles of the user. Depending on

the duration of uninterrupted work, asthenopia

and musculoskeletal problems may occur. For

this reason, appropriate measures for reduction

of the workload must be taken:

OOptimal arrangement of workplace, work

assignments and work ﬂow (changing tasks

frequently).

OThorough training of the personnel, giving

consideration to ergonomic and organiza-

tional aspects.

OThe ergonomic design and construction of

the Leica M stereo microscopy series are

intended to reduce the exertion of the user

to a minimum.

Leica M Series User Manual 7

Safety Instructions (Continued)

•Direct contact with eyepieces is a

potential transmission method for

bacterial and viral infections of the eye.

The risk can be kept to a minimum by using

personal eyepieces for each individual or

detachable eyecups.

Technical safety

To connect the Leica M Series microscopes to

power, use a power cable that meets the power

cable speciﬁcations in your country. The power

socket used has to be equipped with a 16 A or

10 A fuse. Only connect the instrument to a

grounded power socket that has been installed

according to regulations. The power supply

voltage has to correspond to the voltage speci-

ﬁed on the identiﬁcation label. Make sure that

the ground connection is neither defective

nor interrupted, since otherwise a malfunction

poses a risk of fatal injury. Never unplug the

power plug with moist or wet hands. There is

danger of an electric shock.

Instrument safety and EMC

Our device has been designed, produced and

tested in compliance with

OIEC EN 61010-1: Electrical equipment for

measurement, control and laboratory use

– Safety – Part one: general requirements

OEN 60950-1: Information technology

equipment – Safety – Part one: general

requirements

ORadio interference suppression in compli-

ance with EN 55011 class B

ONoise immunity in compliance with

DIN EN 61326-1.

The device meets the requirements of EU

directives

O2014/35/EU Low voltage directive

O2014/30/EU EMC directive

O2011/65/EU RoHS directive

and carries the CE mark.

The device must be disposed of in

accordance with 2012/19/EU WEEE

directive.

Intended for indoor use only in all EU member

states, EFTA states and Switzerland.

Leica M Series User Manual 8

Safety Instructions (Continued)

Handling electrical components

Never install any other plug (NEMA

5-15P) or unscrew any mechanical

components unless expressly instructed to do

so in the instructions.

The microscope must be connected to a

grounded outlet.

Always position the microscope so that

you can disconnect it from the power

supply at any time. The power cable is provided

as the power disconnect device.

Leica M Series User Manual 9

Contents

Leica IsoPro Manual XY Stage: Assembly 31

Cables: Connections 34

Cables: Cable Duct 35

Cables: Diagram 36

Leica LED5000 MCI 37

Leica LED5000 MCI: Alternative Assembly 38

Leica LED5000 RL: Assembly 39

Leica LED5000 RL: Installing Optional Accessories 41

Leica LED5000 RL: Use 42

Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes

(Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) 44

Leica LED5000 NVI: Installing Optional Accessories 47

Quick Start Guide

The Fastest Route to Success 50

Overview of an M Series Microscope 51

The Correct Interpupillary Distance 52

Using the Eyepieces 53

Focusing 54

Adjusting the Resistance of the Focus Drive 55

Changing the Magniﬁcation (Zoom) 56

Ratchet Steps and Magniﬁcation Levels 57

Parfocality: More Comfort and Convenience for Your Work 58

Iris Diaphragm 59

General Instructions 2

Safety Concept 3

Symbols Used 4

Safety Instructions 5

Congratulations! 11

A Step Towards Inﬁnity 12

The Electronics: Comfort, Convenience and Safety for Your Experiments13

The Modular Design: Everything is Relative 14

Maximum Compatibility 15

On We Go 16

Assembly

Base and Focusing Column 18

Assembling the Focusing Column for TL Bases 19

Assembling the Focusing Column With an Incident-light Base 20

Optics Carrier 21

Tube 22

Eyepieces 23

Objective 24

Objective Nosepiece – Assembly 25

Objective Nosepiece – Adjusting Parfocality 26

AX Carrier – Preparation 27

AX Carrier – Assembly 28

Transmitted-light Base Leica TL BFDF: Before First Use 29

Transmitted-light Base Leica TL BFDF 30

Leica M Series User Manual 10

Eyepieces

Magniﬁcation Factors of the Eyepieces 61

Health Notes 62

Dioptric Correction 63

Dioptric Correction and Parfocality 64

Graticules 65

Photography & Video

Photography & Video 67

Photo Tubes and C-mounts 68

Trinocular Video/Phototube 50 % 69

Trinocular Video/Phototube 100 % 70

Trinocular Video/Phototube 100 %: ErgoTube 5°– 45° 71

Microscope Carrier

The Microscope Carrier AX 73

The Objective Nosepiece 75

Objectives and Optical Accessories

The Diﬀerent Types of Objectives 77

Bases

Transmitted-light Base Leica TL BFDF: Controls 79

Transmitted-light Base Leica TL BFDF: Operation 80

Leica IsoPro (Non-motorized): Controls 81

System Illumination

Leica LED5000 MCI 83

Leica LED5000 RL 85

Accessories

Leica Hand Wheel and Foot Switch 87

Leica SmartTouch 88

Dimensional Drawings

Leica M125 C / Leica M165 C 90

Leica LED5000 MCI 92

Leica LED5000 RL 93

Leica LED5000 NVI 94

Leica MATS TPX 95

Speciﬁcations

Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A 97

Transmitted-light Base Leica TL BFDF 101

Motorized Focus Drives With "Zoom" Carrier

(M125 C, M165 C, M205 C, M205 A) 102

Appendix

Calculating the Total Magniﬁcation and Field of View Diameter 104

Care, Maintenance, Contact Persons 105

Contents (Continued)

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