CORNING AXYGEN AxySpin R Manuel utilisateur

Instruction Manual

AxySpin R

Refrigerated Microcentrifuge

Cat. Nos.

601-05-031 – 120 US

601-05-021-230 – EU/UK

601-05-011 – 100 US

Axyspin R Refrigerated Microcentrifuge

About This Manual

This manual is designed to assist you in the optimal usage of your

Axygen® Axyspin R refrigerated microcentrifuge. The manual is

available in English, French, German, Italian, Portuguese, and Spanish

on our website at www.corning.com/lifesciences.

Table of Content

1. Safety Precautions....................................1

1.1. Operation Safety Precautions.............1

2. General Description..................................2

2.1. Features .............................................2

3. Getting Started..........................................2

3.1. Unpacking...........................................2

3.2. Initial Operation...................................3

3.3. Opening and Closing the Lid...............3

3.4. Loading the Rotor ...............................3

3.5. Starting and Stopping a Run ...............4

3.6. Emergency Lid Release......................4

4. Overview....................................................5

5. Operation...................................................7

5.1. Setting the Time (TIME)......................7

5.2. Setting the Speed (RPM) ....................7

5.3. Setting the Centrifugal Force (RCF)....7

5.4. Setting the Temperature (TEMP).........7

5.5. Cooling ...............................................8

5.6. Quick-Spin Operation..........................8

5.7. Changing the Settings During a Run...8

6. Service and Maintenance.........................8

6.1. Centrifuge Service ..............................9

6.2. Cleaning the Centrifuge......................9

6.3. Cleaning the Rotor..............................9

6.4. Disinfection.........................................9

6.5. Replacing the Rotor............................9

6.6. Replacing fuses ..................................9

7. Troubleshooting......................................10

Appendix A: Technical Specifications..........11

Appendix B: Warranty....................................12

AxySpin R™ Refrigerated Microcentrifuge

1. Safety Precautions

Before using the AxySpin R refrigerated microcentrifuge for the first time, please read this entire

operating manual carefully. To guarantee problem free, safe operation, it is essential to observe the

following points:

1.1.Operation Safety Precautions

When using infectious, radioactive, toxic and other solutions which may pose health risks, please

observe the appropriate safety precautions.

Do not use this machine in a potentially explosive environment or with potentially explosive

chemicals.

Install the machine in location free of excessive dust.

Avoid placing the machine in direct sunlight.

Choose a flat, stable surface capable of withstanding the weight of the machine.

Install the machine in the room temperature 15~30°C, relative humidity 20~80%.

Don’t block the air vents.

Make sure the power source conforms to the required power supply specifications.

To avoid electric shock, make sure the machine is plugged into a grounded electric outlet.

Do not allow water or any foreign objects to enter the various openings of the machine.

Switch off the power switch before cleaning or performing any service on the machine, such as

replacing the fuses.

The rotor and the rotor lid must always be installed securely on the motor shaft.

Always load the rotor symmetrically. Each tube should be counterbalanced by another tube

of the same type and weight.

To guarantee sufficient ventilation, ensure that the centrifuge has at least 30 cm of free

space on all sides, including the rear.

Repair should be carried out by Axygen Scientific' authorized service personnel only.

Use original spare parts and accessories only.

Warning Label: Please be aware of the danger of electric shock or other dangers.

2. General Description

The AxySpin R refrigerated microcentrifuges is a compact, bench-top centrifuge which is suitable for

general laboratory use.The motor is brushless and requires no routine maintenance. It is supplied

with a 24 x 1.5/2.0 ml rotor for micro samples. The maximum speed of AxySpin R is 13,500 rpm

(17,135 rcf). The temperature range is from –10°C to 40°C.

2.1.Features

Brushless motor drive, control speed up to 13,500rpm/17,135xg

Powerful refrigeration system, maintain 4°C at max. speed

Includes unique easy access rotor (24 x 1.5ml)

Exceptionally quiet and compact

Optional StripSpin adapter available for 0.2ml tubes and strips

Compact, ergonomic design

User-friendly operation interface.

Quick button for instant run without setting

Low noise level

Complies with CE, RoHS

3. Getting Started

3.1.Unpacking

The centrifuge is delivered in an external carton and an internal carton with protective PE foam

cushions. Remove the centrifuge from each carton. All packaging should be retained until it has

been established that the centrifuge is working properly.

Open the AxySpin R refrigerated microcentrifuge package and confirm that all items are included:

AxySpin R unit with a 24 x 1.5/

2ml

rotor and a rotor lid

Operation manual



Electric fuses (7A &3.15A)



Power cord



Rotor wrench



If there are any items missing, damaged, or not according to your order, please contact your

distributor or sales representative immediately.

Note: Please use 7A fuse in power source 100~120/50~60Hz countries, while 3.15A fuse is for power

source 200~230V/50~60Hz countries.

AxySpin R™ Refrigerated Microcentrifuge

3.2.Initial Operation

Place the machine on a stable, flat table. Please keep the machine at least 30 cm from the

surrounding area or objects.

Mains power and frequency “MUST”be compatible with the information given on the product label on

the rear of the centrifuge.

Connect the power cord to the power socket of the centrifuge, then to the power source. Switch the

Power On/Off switch to turn on the centrifuge. After the LCD display is on and the buzzer beeps twice,

the centrifuge is ready to operate.

3.3.Opening and Closing the Lid

The lid can be opened only when the centrifuge is powered on. Press button to open the lid.

The lid will be locked automatically when the user presses down the front edge of the lid.

Note: Do not slam the lid!

3.4.Loading the Rotor

For safety reasons, the rotor must be loaded with centrifuge tubes symmetrically. The difference in

the weight between the tubes should not exceed 0.1 gram.

The centrifuge tubes should be loaded in pairs opposite each other and each should contain roughly

the equal weight of sample. One or two additional loaded tubes may need to be added to achieve this.

Please refer Figure 1 to see an example of a properly loaded rotor.

Attention: To reduce air friction and noise, the rotor lid should be attached to the rotor during

centrifugation. Make sure that the rotor lid snaps securely into place, by pressing down on

center catch.

Figure 1. Loading the rotor

3.5.Starting and Stopping a Run

Please refer Chapter 5 Operation to set up your preferred settings of time, speed and temperature.

To start a run, press down the (START/STOP) knob.

When the pre-set time expires, the centrifuge will stop automatically. To stop the centrifuge prior to

the expiration of set time, press the (START/STOP) knob again.

When the time is set as “continuous”( - - ), the centrifuge will run continuously. To stop the

continuous run, press the (START/STOP) knob.

By pressing and holding the button, the centrifuge will continue a short-run of set speed. The

short-run will stop if the button is released.

Note:

1. For safety reasons, the (START/STOP) knob will be inactivated when the lid is open. A

symbol “” will be flashing until the lid is closed.

3.6.Emergency Lid Release

When the lid can’t be opened by pressing the button, users can open the lid manually. First, turn

off the power of the centrifuge. Second, use a flat-head screw driver or other tool to remove the

Emergency Lid Release Plug ( Please refer Figure 2 ) on the right-hand side of the machine. Third,

pull the wire (attached to the plug) while gently pushing downward on the lid. The lid will now

release.

Securing

screw

AxySpin R™ Refrigerated Microcentrifuge

4. Overview

This section presents an overview of the AxySpin R microcentrifuge’s various components and

control panel (see Figure 2) as well as the symbols and indicators on the LCD display (see Figure 3).

Figure 2. AxySpin R refrigerated microcentrifuge overview

Figure 3. LCD display panel

Keypad Function

Button Symbol Function Description

START/STOP

and

Value

Adjustment

Knob

Start/Stop a run by pressing

down



Rotate to Increase/decrease the

values of time, temperature,

speed

or g force

TIME button To adjust run time setting

RPM button

To adjust speed setting

RCF button

To adjust RCF setting

Air vents

Power On/Off switch

& fuse box

Emergency lid

release plug

Lid lock hole

START/STOP

knob

TEMP button

To adjust temperature setting

LID button

Open the lid

QUICK button

Quick-Spin centrifugation

Audible Signals

The audible signals are beneficial when you are familiarizing yourself with the operating procedure of

the AxySpin R refrigerated microcentrifuge. They can also be disabled if desired by pressing

button six times. The audible signal function can be resumed by pressing button six times again.

Definition of the audible sounds are shown in the table below.

Audible Sound

Operation

2 beeps

Power on machine

3 beeps

End of a run

AxySpin R™ Refrigerated Microcentrifuge

5. Operation

Users can pre-set the time, temperature and speed (RPM/RCF) before a run.

Attention: Never attempt to operate the centrifuge with rotors or adapters that show signs of

corrosion or mechanical damage. Never centrifuge strongly corrosive materials that may

damage the rotors, accessories or bowl of the unit.

5.1.Setting the Time (TIME)

Operating time can be selected from 0.5 min to 99 min or “continuous”. Press button to change

the time setting. The 2 digits of TIME on the display will flash. Turn the (START/STOP) knob to

increase or decrease the value. Press button again to confirm the selection or press

(START/STOP) knob to store and run the selection. The flashing of time value will stop and the

time value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

Operating time can be set as “continuous” by turning the (START/STOP) knob clockwise or

counter-clockwise to make the 2 digits of TIME to display “- -“. When the time is set as “Continuous”,

the centrifuge will continue to run without a stop. Press (START/STOP) knob to end the

continuous operation.

5.2.Setting the Speed (RPM)

The speed (RPM) can be selected from 500 to 13,500 rpm. Press button to change the speed

setting. The 5 digits of speed (RPM) on the display will flash. Turn the (START/STOP) knob to

increase or decrease the value. Press button again to confirm the selection or press

(START/STOP) knob to store and run the selection. The flashing of RPM value will stop and the

RPM value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

5.3.Setting the Centrifugal Force (RCF)

The speed can be selected from 20 to 17,200 x g, too. Press button to change the RCF setting.

The 5 digits of RCF on the display will flash. Turn the (START/STOP) knob to increase or

decrease the value. Press button again to confirm the selection or press (START/STOP)

knob to store and run the selection. The flashing of RCF value will stop and the RCF value will be

stored automatically if no button is pressed after 6 seconds of the adjustment.

5.4.Setting the Temperature (TEMP)

The temperature can be selected from -10 to 40°C. Press button to change the temperature

setting. The 2 digits of temperature on the display will flash. Turn the (START/STOP) knob to

increase or decrease the value. Press button again to confirm the selection or press

(START/STOP) knob to store and run the selection. The flashing of TEMP value will stop and the

value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

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