Motic SWIFTLINE MAE-31R Manuel utilisateur

Inverted Microscope

Instruction Manual

Model MAE-31R

 
2 
 
TABLE OF CONTENTS 
 
SECTION 
 
II Specifications ................................................................................................................4 
III Setting-up the Instrument ..............................................................................................5 
IV Assembling the microscope Input voltage .....................................................................6 
1Installing the lamp.................................................................................................................... 6 
2Mounting the condenser........................................................................................................... 6 
3Installing Objectives................................................................................................................. 7 
4Mounting the eyepieces........................................................................................................... 7 
 
VMicroscopic procedure Interpupillary distance adjustment ............................................7 
1Diopter adjustment................................................................................................................... 8 
2Centering the Condenser......................................................................................................... 8 
3Centering the lamp................................................................................................................... 9 
4Brightfield microscopy.............................................................................................................11 
5Phase-contrast microscopy.....................................................................................................11 
 
VI Photo Procedure..........................................................................................................13
VII Troubleshooting Table.................................................................................................15 
VIII Care and maintenance ................................................................................................16
1Lenses and filters....................................................................................................................16 
2Cleaning of painted or plastic components..............................................................................16 
3When not in use......................................................................................................................16 
 
IX Warranty......................................................................................................................17

1. Lamp Socket Clamp Screw Knob 8. Objectives

2. Lamp House Cover Clamp Screw 9. Revolving Nosepiece

3. Annular Diaphragm 10. Coaxial Coarse/Fine Focus Knob

4. Condenser Diaphragm Lever 11. Torque Adjusting Ring

5. Condenser Focus Knob 12. Filter Retaining Ring

6. Condenser Lens 13. Hexagonal Centering Screwdrivers (x2)

7. Stage Plate Insert

1. Vertical Photo Port

2. Interpupillary Distance Scale

3. Diopter Adjustment Ring

4. Eyepiece

5. Optical Path Selector Lever

6. Filter Slider

7. Lamp House

8. Field Diaphragm Lever

9. Condenser Clamp Screw

10. Phase Slider

11. Condenser Clamp Holder Screw

12. Condenser Centering Screws

13. Stage Plate

14. Fluorescence Filter cassette Mount

15. Power Switch

16. Light Intensity Control Dial

17. Brightness Indicator (LED Segmented Display)

II Specifications

•Magnification Ratio: 40X –400X

•Eyepiece: N-WF 10X/22mm with diopter adjustment, +/- 5 diopter

•Objectives: Infinity Corrected

Magnification

N.A.

W.D. (mm)

Plan Achromat 4X

0.10

23.5

Plan Achromat Phase 10X

0.25

7.5

Plan Achromat Phase LWD 20X

0.40

7.0

•Condenser: Extra Long Working Distance N.A. 0.3 / W.D. 72mm

•Electrical Specifications:

Input: IEC 90-240V~, 35W, 50-60Hz, 0.6A Max (UL / CE)

Lamp: 6V, 30W Halogen (100hrs)

Fuse: 250V T2.5A

III Setting-up the Instrument

Working environment

•The location should be free from dust, moisture, chemical vapors and mechanical vibrations.

•Do not situate the instrument in a warm and/or humid environment.

•Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp or a

well-lit bright wall. The quality of the viewed image from the microscope will deteriorate where there is

significant ambient light.

IV Assembling the microscope Input voltage

•Automatic voltage selection works with electrical outlets worldwide. However, always use a power cord

that is rated for the voltage used in your area and that has been approved to meet local safety

standards. Using the wrong power cord could cause fire or equipment damage.

•The use of an extension cord is discouraged and may damage the electrical components of the

microscope.

•In order to prevent electric shock, always turn the power switch on the power supply off before

connecting the power cord.

1 Installing the lamp

•In order to prevent electric shock always turn the power switch off and unplug the power cord before

replacing the lamp.

•Loosen and remove the lamp housing cover clamp screw using a coin. Then remove the lamp house

cover.

•When installing the lamp, do not touch the glass surface of the

lamp with bare fingers. Doing so

will cause fingerprints, grease, etc., to burn onto the lamp surface,

reducing the illumination provided by the lamp. If surface is contaminated, wipe it clean using lens

tissue.

•Firmly insert the lamp into the socket pinholes until it reaches the limit, be careful not to tilt the

lamp when mounting.

•Close the cover and fasten with it with lamp housing cover clamp screw.

2 Mounting the Condenser

Power Cord

Lamp Housing Cover

Clamp Screw

Power Switch

•Mount the condenser with the index marks and aperture diaphragm lever facing forward. Then secure

it with the clamp screw.

•Insert the phase annular diaphragm slider with centering hexagonal socket head screws facing

the front. See the images above for reference.

3 Installing Objectives

•Remove the stage plate insert from the stage.

•Install the objectives into the nosepiece so that the magnification increases with clockwise rotation of the

revolving nosepiece.

•Replace the stage plate insert.

4 Mounting the eyepieces

•Remove the dust caps from the eyepiece tubes.

•Insert the eyepieces into the eyepiece tubes.

•If the rubber eye guards are to be used, fit them in the groove around the eyepiece.

V Microscopic procedure Interpupillary distance adjustment

•Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.

Condenser Clamp

Screw

Phase Annular

Diaphragm

Slider

Condenser

•Adjust the interpupillary distance so that both the right and left field of view become one.

•This adjustment will enable the user to observe the specimen with both eyes

1 Diopter adjustment

•Diopter adjustment compensates for differences in vision between the left and right eyes. In addition to

making observation through both eyes easier, this adjustment also reduces the

extent to which focusing is lost when the objective magnification is changed. In particular,

this occurs when a low power objective is used.

•Before adjusting the diopter, bring a specimen into focus using the 10x objective.

•Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to

“0” position.

•Position 40x objective into the optical path and bring the specimen image into focus by turning

the coarse and fine focus knobs.

•Position either 4x or 10x objective into optical path. Without adjusting the fine and coarse focus knobs,

turn the diopter rings on the eyepieces so that the specimen images in the left and right eyepieces are

focused individually.

•Repeat the above step twice.

2 Centering the Condenser

•Set position of the phase annular diaphragm slider in center opened position, of the condenser.

•Fully open the condenser diaphragm by moving lever to the open “O” position.

Diopter adjustment “0”

position

Center Open Position

•Turn the light intensity down and switch to the 10x objective.

•Close the field of view diaphragm, 3/4 of the way. This lever is located on the lamp housing. This will

close down the iris diaphragm in the lamp housing and become visible through the eyepiece field of

view.

•Turn the condenser focus knob up or down until the iris edges sharpen in the field of view.

•Adjust the condenser centering screws so that the center of the field diaphragm image matches the

center of the field of view. This adjustment is easier to make if the field diaphragm size is reduced

slightly smaller than the eyepiece field of view.

•IMPORTANT: For normal observation, the edges of the

diaphragm should be just outside the field of view.

3 Centering the lamp

•Set the Phase annular diaphragm slider in the open center position.

Condenser centered

Center Open Position

Condenser not centered

Condenser centered

Condenser Centering Screws

•Close the condenser diagram down completely –moving lever to C.

•Fully open the field of diaphragm and

remove the diffuser filter slide.

•Loosen, don’t remove the lamp socket clamp screw using the knob. This will allow the knob to move

around with little resistance.

•Holding the lamp socket clamp screw, move the lamp filament image to the center of the condenser

diaphragm iris. The filament will be visible looking down from above through the top of the condenser.

Once centered tighten down the lamp socket clamp screw.

•After finishing the above lamp centering procedure, re-insert the filter slider.

Lamp Socket Clamp

Screw Knob

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