Agilent Technologies G1701EA Manuel utilisateur

Agilent Technologies

Agilent G1701EA MSD

Productivity

ChemStation

Familiarization Guide

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from

Agilent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G1701-90070

Edition

Second edition, June 2012

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Boulevard

Santa Clara, CA 95051 USA

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

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liable for errors or for incidental or

consequential damages in connection

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mance of this document or of any

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Agilent and the user have a separate

written agreement with warranty

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document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or sub-

contract, Software is delivered and

licensed as “Commercial computer soft-

ware” as defined in DFAR 252.227-7014

(June 1995), or as a “commercial item” as

defined in FAR 2.101(a) or as “Restricted

computer software” as defined in FAR

52.227-19 (June 1987) or any equivalent

agency regulation or contract clause. Use,

duplication or disclosure of Software is

subject to Agilent Technologies’ standard

commercial license terms, and non-DOD

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Government will receive no greater than

Restricted Rights as defined in FAR

52.227-19(c)(1-2) (June 1987). U.S. Govern-

ment users will receive no greater than

Limited Rights as defined in FAR 52.227-14

(June 1987) or DFAR 252.227-7015 (b)(2)

(November 1995), as applicable in any

technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an oper-

ating procedure, practice, or the

like that, if not correctly performed

or adhered to, could result in

damage to the product or loss of

important data. Do not proceed

beyond a CAUTION notice until the

indicated conditions are fully

understood and met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and met.

Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 3

In This Guide

This guide contains a step-by-step exercise to help you become

familiar with your Agilent 7890A GC/5975 MSD with G1701EA

MSD Productivity ChemStation software.

To successfully use this book, you need the following:

Before operating your instruments, be sure to read all safety

and regulatory information included with your instruments.

1Start Up the System

Start up your system hardware and software for data

acquisition.

2Tune the MS

Determine whether the instrument is correctly tuned.

3Create a Method for Qualitative Analysis

Create a new qualitative analysis scan method from the system

default method.

4Run the Scan Method

Run the method created in Chapter 3to acquire sample data.

5Qualitative Data Analysis

Use the Enhanced Data Analysis program to analyze the data

generated in Chapter 4.

6Create a SIM Quantitation Method

Create a SIM method from the scan method created in

Chapter 3.

7Run a Sequence

Create and run a sequence using the method created in

Chapter 6.

•GC Inlet: Split/Splitless Inlet with EPC

(default inlet configuration)

•Column: HP-5ms 30 m x 250 µm x 0.25 µm

•Sample: 5975 MSD Sample (P/N 05970-60045) or

(P/N 5074-3025 Japan only)

•MSD Tuning

Calibrator:

PFTBA (perfluorotributylamine)

4 Agilent G1701EA MSD Productivity ChemStation

8Set Up a Quantitation Database

Set up a database with compounds and calibrators to identify

unknown samples.

9Generate a Report

Generate a report automatically after a run or at a later point

from previously acquired data.

10 Recalibrate and Quantitate Unknowns

Modify a sequence for recalibration and then use it to

quantitate an unknown sample.

11 Create a Cool Down Method

Create and store a maintenance method.

12 Shut Down the System

13 Frequently Asked Questions

Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 5

Where to Find Information

Hardware

In addition to this document, Agilent provides several learning

products that document how to install, operate, maintain, and

troubleshoot the 7890A GC/5975 MSD. This information can be found

on the Agilent Technologies GC and GC/MS Hardware User

Information and Utilities DVDs that ship with your instrument.

The Agilent Technologies GC and GC/MS Hardware User Information

and Utilities DVDs that ship with your instrument provides an

extensive collection of online help, videos, and books for current

Agilent gas chromatographs, mass selective detectors, ion traps, and

GC samplers. Included are localized versions of the information you

need most, such as:

•Getting Familiar documentation

•Safety and Regulatory guides

•Site Preparation checklists

•Installation information

•Operating guides

•Maintenance information

•Troubleshooting details

Software

For an introduction to, and where to find more information on,

the G1701EA MSD Productivity ChemStation see the Agilent

G1701EA GC/MSD ChemStation Getting Started manual.

6 Agilent G1701EA MSD Productivity ChemStation

Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 7

Contents

1 Start Up the System

Start up the Hardware 12

Run the ChemStation Software 14

Select the Tune File 15

Load the Method 16

2 Tune the MS

Introduction 18

Run Autotune 19

Evaluate the Autotune Results 22

Tune History Trends 24

3 Create a Method for Qualitative Analysis

Introduction 26

Edit the Entire Method 27

Check the GC configuration 29

Set the GC readiness state 32

Set the GC oven parameters 33

Set the GC column parameters 35

Set the GC inlet parameters 36

Set the GC injector parameters 38

Set the GC Aux heaters parameters 40

Set the GC signals parameters 40

Edit the GC real time plots to display 42

Edit the MS parameters 42

Save the method 46

General Information for Editing the GC Parameters 47

Open the GC edit parameters window 47

Add a column to ChemStation local inventory 48

Select and configure a column 51

Upload parameters from the 7890A GC 53

Customize the status panel view 53

8 Agilent G1701EA MSD Productivity ChemStation Familiarization Guide

4 Run the Scan Method

Prepare the Sample 56

Load the Method 57

Run the Method 58

Take a Snapshot 61

View the Logbook 62

5 Qualitative Data Analysis

Integrate Peaks 66

Edit the integration events 69

Save the integration events to the method 70

Manually integrate peaks 71

View the integration results in a table 72

Edit the Method to Generate a Report 74

Display Extracted Ion Chromatograms (EIC)s 76

Enable or Disable the Right Mouse Click Context Menu 78

Analyze Data 79

Subtract the baseline noise from the spectra 81

Select target and qualifier ions 82

Search the Spectral Library 83

Generate an automated library search report 84

Print a Window, TIC, Spectrum, or Method 86

Select a printer 86

Select an item to print 87

Save the Data Analysis Method 87

Exit the Data Analysis Program 88

6 Create a SIM Quantitation Method

Introduction 90

Create a SIM Method 91

Simultaneously Acquire Scan and SIM Data (SIM/Scan Mode) 96

SIM/Scan Mode Cycle Frequency 98

7 Run a Sequence

Prepare the Samples 100

Create the Sequence 101

Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 9

Save the Sequence 103

Load the Sequence 104

Run the Sequence 105

Print the Sequence Log 106

8 Set Up a Quantitation Database

Add Compound Entries for the Database 108

Identify compounds 112

Add the Calibration Curve 115

Add calibrator level 1 115

Add calibrator levels 5, 10, 25, and 50 to the calibration curve 117

Save the database 119

View or Edit an Existing Database 120

9 Generate a Report

Generate a Report Automatically After the Run 124

Load the method 124

Edit the method to generate a report 124

Run the method and generate the report 127

Generate a Detailed Report for Previously Acquired Data 129

Load the method 129

Load the data file 129

Generate a detailed quantitation report 129

10 Recalibrate and Quantitate Unknowns

Create a Recalibration Sequence 132

Save the Sequence 134

Run the Sequence 135

11 Create a Cool Down Method

Create the Cool Down Method 138

Use the Cool Down Method 139

12 Shut Down the System

Shut Down the MS 142

Shut Down the GC 143

13 Frequently Asked Questions

10 Agilent G1701EA MSD Productivity ChemStation Familiarization Guide

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