Agilent Technologies 6475 Manuel utilisateur

Agilent 6475 Triple Quad LC/MS System

User Guide

Notices

Document Identification

DocNo D0025685

DE22440912

October 2022

No part of this manual may be

reproduced in any form or by any means

(including electronic storage and retrieval

or translation into a foreign language)

without prior agreement and written

consent from Agilent Technologies, Inc.

as governed by United States and

international copyright laws.

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

Warranty

The material contained in this document

is provided “as is,” and is subject to being

changed, without notice, in future

editions. Further, to the maximum extent

permitted by applicable law, Agilent

disclaims all warranties, either express or

implied, with regard to this manual and

any information contained herein,

including but not limited to the implied

warranties of merchantability and fitness

for a particular purpose. Agilent shall not

be liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or performance

of this document or of any information

contained herein. Should Agilent and the

user have a separate written agreement

with warranty terms covering the

material in this document that conflict

with these terms, the warranty terms in

the separate agreement shall control.

Technology Licenses

The hardware and/or software described

in this document are furnished under a

license and may be used or copied only in

accordance with the terms of such

license.

Restricted Rights Legend

U.S. Government Restricted Rights.

Software and technical data rights

granted to the federal government

include only those rights customarily

provided to end user customers. Agilent

provides this customary commercial

license in Software and technical data

pursuant to FAR 12.211 (Technical Data)

and 12.212 (Computer Software) and, for

the Department of Defense, DFARS

252.227-7015 (Technical Data -

Commercial Items) and DFARS

227.7202-3 (Rights in Commercial

Computer Software or Computer

Software Documentation).

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly

performed or adhered to, could

result in damage to the product or

loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated

conditions are fully understood

and met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly

performed or adhered to, could

result in personal injury or death.

Do not proceed beyond a

WARNING notice until the

indicated conditions are fully

understood and met.

Software Revision

This guide is valid for MassHunter 12.0,

until superseded.

Instrument Manufacturing

Manufactured by Agilent

Technologies Singapore Pte. Ltd.

No. 1 Yishun Avenue 7, Singapore

768923

Operating Temperature

Operating Temperature: 15°C to 35°C

Storage Temperature: -40°C to 70°C

Agilent 6475 Triple Quad LC/MS System User Guide 3

Content

1AboutthisSystem 6

Sustainability Information 7

Packaging End-of-Life 7

Product End-of-Life 7

Agilent Regulatory Compliance Statement 8

CE Compliance 8

UK Compliance 9

Electromagnetic Compatibility 10

EMC Declaration for South Korea 11

Detachable Power Cord Declaration for Japan 11

Sound Emission Certification for Federal Republic of Germany 11

Waste Electrical and Electronic Equipment (WEEE) Directive 12

Technical Support 13

Agilent Community 13

2 Hardware Overview 14

6475A LC/TQ 15

Front view 15

Calibrant Delivery System 16

Side view 17

Instrument Status Indicator Lights 18

Instrument Status Indicators in Data Acquisition 19

Modes of Operation 20

ON mode 20

OFF mode 20

Standby mode 21

Ion Sources 22

Agilent Jet Stream (AJS) source 23

Electrospray Ionization (ESI) source 24

Atmospheric Pressure Chemical Ionization (APCI) source 25

Multimode Ionization (MMI) source 26

Content

Agilent 6475 Triple Quad LC/MS System User Guide 4

3Tuning 27

About Tuning 28

Autotune and Checktune 28

Starting the Instrument 29

Calibrating the LC/TQ (Checktune) 30

Calibrating the LC/TQ (Autotune) 31

Generating a Detailed Tune Report 32

Customizing the Actuals Window 33

Scheduling a Checktune 34

Scheduling an Autotune 35

Monitoring Baseline and Adjust Plot 36

Adjusting the plot in any of the following ways: 36

Fixing Spray Stability EMF 37

4 Basic Operation 38

Starting Up and Shutting Down the Instrument 39

Starting in Standby mode 39

Turning off the instrument 39

Starting the Data Acquisition Software 41

Data Acquisition Windows 42

Preparing the LC Modules 46

Preparing the Triple Quad LC/MS 50

Performing a Checktune or Autotune 50

Switching LC stream to MS 50

Monitoring MS baseline and spectral displays 50

Viewing the system logbook for events and errors 51

Content

Agilent 6475 Triple Quad LC/MS System User Guide 5

Setting Up and Running an Acquisition Method 52

Reviewing Results with Qualitative Analysis 58

Analyzing Data with Quantitative Analysis 59

5 Maintenance 60

Routine Tasks 61

Calibrant Delivery System and Divert Value 61

Sources 62

Nebulizer 65

Periodic Tasks 66

Calibrant Delivery System and Divert Value 66

Sources 67

Nebulizer 73

Corona Needle 78

Ion Injector 80

Rough Pump 83

Disinfection 89

Agilent 6475 Triple Quad LC/MS System User Guide 6

1AboutthisSystem

Sustainability Information 7

Agilent Regulatory Compliance Statement 8

Technical Support 13

1AboutthisSystem

Sustainability Information

Agilent 6475 Triple Quad LC/MS System User Guide 7

Sustainability Information

Packaging End-of-Life

The Agilent 6475 triple quadrupole LC/MS system is packaged using cardboard

and a polyethylene bag. In the US, the cardboard is readily recyclable; while the

plastic bag is recyclable, the infrastructure is not available in the majority of the

market. In the US, Agilent is enrolled in the How2Recycle program to facilitate the

communication of material recycling instructions. In select countries, Agilent

participates in the EU/UK Green DOT Package Recycling Program to support the

take-back and responsible management of the packaging materials at their

end-of-life. In the remaining EU countries, the cardboard is readily recyclable

while the plastic bag is recycled, landfilled or incinerated depending on the

country and its available infrastructure.

Product End-of-Life

Agilent offers a take-back program for its 6475 triple quadrupole LC/MS system

customers in US, EU and UK markets.

1AboutthisSystem

Agilent Regulatory Compliance Statement

Agilent 6475 Triple Quad LC/MS System User Guide 8

Agilent Regulatory Compliance Statement

CE Compliance

Your Agilent instrument has been designed to comply with the requirements of

the applicable directives of the European Union, such as Electromagnetic

Compatibility (EMC) Directive, Low Voltage Directive (LVD), Machinery Directive

(MD), RoHS Directive, etc.

Agilent has confirmed that each product complies with the relevant Directives by

testing samples against the harmonized EN (European Norm) standards

published on the Official Journal of the European Union (OJEU).

Proof that a product complies with these directives is indicated by:

•the CE Marking appearing on the rear of the product, and

•the documentation package that accompanies the product containing a copy

of the Declaration of Conformity. The Declaration of Conformity is the legal

declaration by Agilent that the product complies with the relevant directives

listed above, and shows the EN standards to which the product was tested to

demonstrate compliance.

1AboutthisSystem

UK Compliance

Agilent 6475 Triple Quad LC/MS System User Guide 9

UK Compliance

Your Agilent instrument has been designed to comply with the requirements of

the applicable regulations of the United Kingdom, such as The Electromagnetic

Compatibility Regulations 2016, The Electrical Equipment (Safety) Regulations

2016, The Supply of Machinery (Safety) Regulations 2008, The Restriction of the

Use of Certain Hazardous Substances in Electrical and Electronic Equipment

Regulations 2012, etc.

Agilent has confirmed that each product complies with the relevant Regulations

by testing samples against the designated standards published on GOV.UK.

Proof that a product complies with these regulations is indicated by:

•the UKCA Marking appearing on the rear of the product, and

•the documentation package that accompanies the product containing a copy

of the Declaration of Conformity. The Declaration of Conformity is the legal

declaration by Agilent that the product complies with the relevant regulations

listed above, and shows the designated standards to which the product was

tested to demonstrate compliance.

1AboutthisSystem

Electromagnetic Compatibility

Agilent 6475 Triple Quad LC/MS System User Guide 10

Electromagnetic Compatibility

This product conforms to the following regulations on Electromagnetic

Compatibility (EMC) and Radio Frequency Interference (RFI):

•CISPR 11/EN 55011: Group 1, Class A

•EC/EN 61326-1

•AUS/NZ

•Canada ICES-001 (This ISM device complies with Canadian ICES-001. Cet

appareil ISM est conforme à la norme NMB-001 du Canada).

Group 1 ISM equipment Group 1 contains all Industrial, Scientific and Medical

(ISM) equipment in which there is intentionally generated and/or used

conductively coupled radio- frequency energy which is necessary for the internal

functioning of the equipment itself.

Class A equipment This equipment is suitable for use in all establishments

other than domestic and those directly connected to a low voltage power supply

network which supplies buildings used for domestic purposes.

This device complies with the requirements of CISPR11, Group 1, Class A as

radiation professional equipment. Therefore, there may be potential difficulties in

ensuring electromagnetic compatibility in other environments, due to conducted

as well as radiated disturbances.

If this equipment does cause harmful interference to radio or television reception,

which can be determined by turning the equipment off and on, the user is

encouraged to try one or more of the following measures:

1Relocate the radio or antenna.

2Move the device away from the radio or television.

3Plug the device into a different electrical outlet, so that the device and the

radio or television are on separate electrical circuits.

4Make sure that all peripheral devices are also certified.

5Make sure that appropriate cables are used to connect the device to

peripheral equipment.

6Consult your equipment dealer, Agilent Technologies, or an experienced

technician for assistance.

Changes or modifications not expressly approved by Agilent Technologies could

void the user’s authority to operate the equipment.

Table des matières

Autres manuels Agilent Technologies Équipement de laboratoire

Agilent Technologies

Agilent Technologies Magnis NGS Prep System Manuel utilisateur

Agilent Technologies

Agilent Technologies Small RNA Kit Manuel utilisateur

Agilent Technologies

Agilent Technologies 33120A Manuel utilisateur

Agilent Technologies

Agilent Technologies G5562A Guide de configuration

Agilent Technologies

Agilent Technologies HS-16 Manuel utilisateur

Agilent Technologies

Agilent Technologies 7250 Manuel utilisateur

Agilent Technologies

Agilent Technologies E5850A Manuel utilisateur

Agilent Technologies

Agilent Technologies Archon Mode d’emploi

Agilent Technologies

Agilent Technologies Magnis NGS Prep System Manuel utilisateur

Agilent Technologies

Agilent Technologies 1290 Infinity II Manuel utilisateur

Agilent Technologies

Agilent Technologies 990 Micro GC Guide de l'utilisateur

Agilent Technologies

Agilent Technologies SureCycler 8800 Guide de sécurité

Agilent Technologies

Agilent Technologies 1200 Infinity Series Manuel utilisateur

Agilent Technologies

Agilent Technologies PlateLoc Manuel utilisateur

Agilent Technologies

Agilent Technologies 1260 Infinity Manuel utilisateur

Agilent Technologies

Agilent Technologies 6850 Series II Mode d’emploi

Agilent Technologies

Agilent Technologies 1290 infinity Manuel utilisateur

Agilent Technologies

Agilent Technologies N2727A Manuel utilisateur

Agilent Technologies

Agilent Technologies 1168/9A Manuel utilisateur

Agilent Technologies

Agilent Technologies 6100 Series Manuel utilisateur

Agilent Technologies

Agilent Technologies 7697A Manuel d'installation

Agilent Technologies

Agilent Technologies BioCel 1200 Manuel utilisateur

Agilent Technologies

Agilent Technologies 6820 Manuel utilisateur

Agilent Technologies

Agilent Technologies 1260 Infinity II Manuel utilisateur

Manuels Équipement de laboratoire populaires d'autres marques

Qiagen

Qiagen DML 3000 Manuel utilisateur

Queensgate

Queensgate NANOSCAN OP400 Manuel de programmation

Conviron

Conviron GEN1000-GE Manuel utilisateur

Parr Instrument

Parr Instrument 4560 Mode d’emploi

IKA

IKA IC control Manuel utilisateur

Integra

Integra DOSE IT Manuel utilisateur

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manuel utilisateur

NI PXI-5422 Manuel

Collomix

Collomix Aqix Manuel utilisateur

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manuel utilisateur

Ocean Insight

Ocean Insight FLAME-NIR+ Manuel utilisateur

Parker

Parker ALIGN-MG-NA Manuel utilisateur

BD 644787 Manuel utilisateur

DENTAURUM

DENTAURUM Compact Megaplus Manuel utilisateur

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manuel utilisateur

VWR

VWR SAS Super IAQ Manuel utilisateur

illumina

illumina MiSeqDx Manuel utilisateur

Yamato

Yamato BM510 Manuel utilisateur