YSI 550A Manuel utilisateur

Operations

Manual

YSI 550A

Dissolved Oxygen Instrument

YSI 550A Y S I Environmental iii

CONTENTS

CONTENTS............................................................................................................................................iii

GENERAL DESCRIPTION..................................................................................................................1

WARRANTY ..........................................................................................................................................2

FEATURES OF THE YSI 550A...........................................................................................................3

PRINCIPLES OF OPERATION..........................................................................................................5

PREPARING THE PROBE..................................................................................................................5

DISSOLVED OXYGEN CALIBRATION..........................................................................................6

SALINITY COMPENSATION CALIBRATION..............................................................................7

PROBE OPERATION...........................................................................................................................8

PROBE ELECTRODE MAINTENANCE..........................................................................................9

ACCESSORIES/REPLACEMENT PARTS.....................................................................................10

SPECIFICATIONS ..............................................................................................................................11

TROUBLESHOOTING.......................................................................................................................12

CONTACT INFORMATION.............................................................................................................13

REQUIRED NOTICE..........................................................................................................................14

YSI 550A Y S I Environmental Page 1

GENERAL DESCRIPTION

The YSI 550A Handheld Dissolved Oxygen Instrument is a rugged, microprocessor based, digital

instrument with a field-replaceable YSI dissolved oxygen probe. The YSI 550A DO Instrument is

impact-resistant and waterproof.

The YSI 550A DO Instrument is designed for field use and is available with cable lengths of 12, 25, 50,

or 100 feet (3.5, 7.5, 15, 30.5 meters). The body of the probe has been manufactured with stainless steel

to add rugged durability and sinking weight. The large Liquid Crystal Display (LCD) is easy to read

and is equipped with a backlight for use in dark or poorly lighted areas.

The YSI 550A DO Instrument can be easily calibrated with the press of a few keys. Additionally, the

instrument’s microprocessor performs a self-diagnostic routine each time the instrument is turned on.

The self-diagnostic routine provides you with useful information about the function of the instrument

circuitry and the quality of the readings you obtain.

The system displays temperature in either oC or oF and dissolved oxygen in either mg/L (milligrams per

liter) or % air saturation. The system requires only a single calibration regardless of which dissolved

oxygen display is used, and will calibrate in either mode. Salinity compensation values can be changed

at any time without performing a new calibration.

A detachable calibration chamber is mounted to the back of the instrument. A small sponge in the

chamber can be moistened to provide a water saturated air environment that is ideal for air calibration.

This chamber is also designed for transporting and storing the probe. When the probe is stored in the

chamber, the moist environment will prolong effective membrane performance and probe life.

The YSI 550A DO Instrument is powered by 4 C-size alkaline batteries. A new set of alkaline batteries

will provide approximately 2000 hours of continuous operation. If the backlight is used often, batteries

will be depleted faster.

The YSI 550A case is waterproof with an IP-67 rating. The instrument is 100% corrosion proof and can

be operated in a wet environment without damage to the instrument.

INITIAL INSPECTION

When you unpack your new YSI 550A DO Handheld Instrument for the first time, check the packing

list to make sure you have received everything. If there is anything missing or damaged, call the dealer

from whom you purchased the YSI 550A. If you do not know which authorized dealer sold the system

to you, call YSI Customer Service at 800-897-4151 or 937-767-7241.

Page 2 Y S I Environmental YSI 550A

WARRANTY REGISTRATION

Please complete the Product Registration on the YSI website at www.ysi.com. If you are not online,

you may complete the Warranty Card included with your instrument and return it to YSI Incorporated.

Your purchase of this quality instrument will then be recorded in YSI’s customer database. Once your

purchase is recorded, you will receive prompt, efficient service in the event any part of your YSI 550A

DO Instrument should ever need repair.

WARRANTY

The YSI 550A DO Instrument is warranted for three years from date of purchase by the end user

against defects in materials and workmanship. YSI 550A DO probes and cables are warranted for one

year from date of purchase by the end user against defects in material and workmanship. Within the

warranty period, YSI will repair or replace, at its sole discretion, free of charge, any product that YSI

determines to be covered by this warranty.

To exercise this warranty, write or call your local YSI representative, or contact YSI Customer Service

in Yellow Springs, Ohio. Send the product and proof of purchase, transportation prepaid, to the

Authorized Service Center selected by YSI. Repair or replacement will be made and the product

returned, transportation prepaid. Repaired or replaced products are warranted for the balance of the

original warranty period, or at least 90 days from date of repair or replacement.

Limitation of Warranty

This Warranty does not apply to any YSI product damage or failure caused by (i) failure to install,

operate or use the product in accordance with YSI’s written instructions, (ii) abuse or misuse of the

product, (iii) failure to maintain the product in accordance with YSI’s written instructions or standard

industry procedure, (iv) any improper repairs to the product, (v) use by you of defective or improper

components or parts in servicing or repairing the product, or (vi) modification of the product in any way

not expressly authorized by YSI.

THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED,

INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR

PURPOSE. YSI’s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR

REPLACEMENT OF THE PRODUCT, AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE

REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY. IN NO

EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR

CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED

BY THIS WARRANTY.

YSI 550A Y S I Environmental Page 3

FEATURES OF THE YSI 550A

KEYPAD

Powers the unit on or off. The instrument will activate all segments of the display for

a few seconds, and then will show a self-test procedure for several more seconds.

During this power on self-test sequence, it is normal to error messages appear and

disappear. If the instrument were to detect a problem, a continuous error message

would be displayed.

Turns the display backlight on or off. The light will turn off automatically after two

minutes of non-use.

Mode During DO calibration it allows the user to select between % and mg/L. After

selection, it may be pressed several times to exit back to measurement mode without

completing the calibration. During measurement, it switches the instrument display

between DO %, DO mg/L, and salinity calibration.

▲and ▼Increases or decreases the value during calibrations.

▼and Mode Press at the same time to switch the temperature units between Fahrenheit (F) and

Celsius (C).

▲and Mode Press at the same time to increase or decrease the resolution of the instrument in

mg/L or % measurement mode.

BATTERIES

The YSI 550A DO Instrument is powered by 4 C-size alkaline batteries. A new

set of alkaline batteries will provide approximately 2000 hours of continuous

operation. When batteries need to be replaced, the LCD will display a "LO

BAT" message. When the message first appears, the instrument will have

approximately 50 hours of life left, provided the back light is not used.

INSTRUMENT CASE

The waterproof instrument case is sealed at the factory and is not to be opened, except by authorized

service technicians.

Caution: Do not attempt to separate the two halves of the instrument case as this may damage the

instrument, break the waterproof seal, and will void the manufacturer's warranty.

Page 4 Y S I Environmental YSI 550A

CALIBRATION/STORAGE CHAMBER

The YSI 550A DO Instrument has a convenient

calibration/storage chamber that can be attached to the

instrument’s back. The calibration chamber can be

used from either side of the instrument, by moving the

rubber stopper to either end.

If you look into the chamber, you should notice a small

round sponge in the bottom. Carefully put 3 to 6 drops

of clean water into the sponge. Turn the instrument

over and allow any excess water to drain out of the chamber. The wet sponge creates a 100% water

saturated air environment for the probe. This environment is ideal for dissolved oxygen calibration and

for storage of the probe during transport and non-use.

The YSI 550A DO Instrument’s storage chamber can be conveniently used from either side of the

instrument.

1. Remove the chamber from the

instrument by unscrewing the two

screws.

2. Remove the rubber stopper from

the chamber by pulling the “tail”

free of the small hole on the

chamber.

3. Re-attached the rubber stopper to

the storage chamber by threading

the “tail” through the opposite small hole on the chamber.

4. Re-attached the storage chamber to the instrument using the two screws.

HAND STRAP

The hand strap is designed to allow comfortable operation of the YSI 550A DO Instrument with

minimum effort. If the hand strap is adjusted correctly, it is unlikely that the instrument will be dropped

or bumped from your hand. The hand strap can be conveniently used from either side of the instrument.

To switch the hand strap from one side to the other:

1. Pull the two velcro strips apart.

2. Pull the strap free of the upper and lower hooks.

3. Feed the strap through the hooks on the other side of the instrument.

4. Adjust the strap length so that your hand is snugly held in place.

5. Press the two velcro strips back together.

TAIL

SMALL HOLE

FOR STOPPER’S

TAIL

STOPPER

CALIBRATION CHAMBER

YSI 550A Y S I Environmental Page 5

PRINCIPLES OF OPERATION

The sensor consists of a silver body as the anode and a circular gold cathode embedded in the end. In

operation, this end of the sensor is filled with a solution of electrolyte containing a small amount of

surfactant to improve wetting action.

A thin semi-permeable membrane, stretched over the sensor, isolates the electrodes from the

environment, while allowing gases to enter. When a polarizing voltage is applied to the sensor

electrodes oxygen that has passed through the membrane reacts at the cathode causing a current to flow.

The membrane passes oxygen at a rate proportional to the pressure difference across it. Since oxygen is

rapidly consumed at the cathode, it can be assumed that the oxygen pressure inside the membrane is

zero. Hence, the force causing the oxygen to diffuse through the membrane is proportional to the partial

pressure of oxygen outside the membrane. As the oxygen partial pressure varies, so does the oxygen

diffusion through the membrane. This causes the probe current to change proportionally.

PREPARING THE PROBE

MEMBRANE CAP INSTALLATION

The YSI 550A DO probe is shipped with a dry, protective membrane. Before using the instrument for

the first time, remove the protective cap and replace it with a new one following these instructions:

1. Remove the probe sensor guard to access the probe tip.

2. Unscrew and remove the old membrane cap and discard.

3. Thoroughly rinse the sensor tip with distilled or DI water.

4. Fill a new membrane cap with O2probe solution that has been prepared according to the directions

on the bottle. Be very careful not to touch the membrane surface. Lightly tap the side of the

membrane cap to release bubbles that may be trapped.

5. Thread the membrane cap onto the probe. It is normal for a small amount of electrolyte to

overflow.

6. Replace the probe sensor guard.

MEMBRANE MAINTENANCE

Additional membrane changes will be required over time. The average replacement interval is 4 to 8

weeks, although they may last longer if kept clean. To clean the membrane, use a lint-free cloth, such

as a Kimwipe, and rubbing alcohol to gently remove the contamination. In harsh environments, such

as wastewater, membrane replacements may be required every 2 to 4 weeks.

Page 6 Y S I Environmental YSI 550A

DISSOLVED OXYGEN CALIBRATION

Dissolved oxygen calibration must be done in an environment with known oxygen content. The YSI

550A DO Instrument can be calibrated in either mg/L or % saturation. Sections below include

instructions on how to calibrate in either mode.

BEFORE YOU CALIBRATE

To accurately calibrate the YSI 550A, you will need to know the following information:

• The approximate salinity of the water you will be analyzing. Fresh water has a salinity of

approximately zero. Seawater has a salinity of approximately 35 parts per thousand (ppt). If you

are uncertain what the salinity of the sample water is, use a YSI 30 Salinity-Conductivity-

Temperature instrument to determine a salinity value.

• For calibration in % saturation mode, the approximate altitude (in feet) of the region where you are

located is required. This information can be obtained over the internet or from a local airport or

weather station. To convert from meters to feet, divide by 0.3048.

For best results:

• Check calibration with each use and recalibrate as necessary to prevent drift. Dissolved oxygen

readings are only as good as the calibration.

• Calibrate at a temperature within ±10°C of the sample temperature.

CALIBRATION IN % SATURATION

1. Ensure that the sponge inside the instrument's calibration chamber is moist. Insert the probe into

the calibration chamber.

2. Turn the instrument on. Allow it to warm up and readings to stabilize for about 15-20 minutes.

3. Press and release both the UP ARROW and DOWN ARROW keys at the same time to enter the

calibration menu.

4. Press the Mode key until “%” is displayed on the right side of the screen for oxygen units. Press

ENTER.

5. The LCD will prompt you to enter the local altitude in hundreds of feet. Use the arrow keys to

increase or decrease the altitude. When the proper altitude appears on the LCD, press the

ENTER key.

EXAMPLE: Entering the number 12 here indicates 1200 feet.

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