YSI 550A Manuel utilisateur

Water Action Volunteers –Level 2 Manual Updated January 2016

YSI Model 550a Dissolved Oxygen (DO) Meter

The YSI 550a Dissolved Oxygen (DO) meter needs to be calibrated before being used in the field

each and every time you monitor. Once calibrated, the meter must be left on until you/your

team is done with monitoring activities for the day at all monitoring locations.

In order to avoid damaging the meter, do not let sponge inside the probe chamber dry.

Maintaining a damp environment in the chamber will help to ensure that your meter has high

quality performance throughout the field season. Keep sponge moist by re-wetting it, at least

bi-weekly, if not more frequently. If the sensor membrane becomes damaged, you will need to

replace it (contact the WAV coordinator for assistance) and then wait at least 1 hour before

calibrating and using the meter to monitor at your stream site.

Get to know your YSI 550A DO meter

y: displays temperature in either °C or °F

and dissolved oxygen in either mg/L (milligrams per liter) or %

air saturation.

Power button: powers the unit on or off.

Backlight button: turns the display backlight on or off. The

light will turn off automatically after two minutes of non-use.

Mode button: allows the user to select between % and mg/L

during calibration process. When monitoring, pushing ‘Mode’

switches the instrument display between DO %, DO mg/L, and

salinity calibration.

Arrow keys: increases or decreases the value during

calibrations.

: enter button

Probe: use to monitor water temp and dissolved oxygen content. Clean

the probe with DI water after each monitoring event and store in the

calibration chamber when not in use.

Maintaining your YSI 550A DO meter

Verify that the sensor membrane is in good condition by removing the sensor from the storage

chamber and check that the sponge is damp, if there are holes or tears in the membrane, and

for air bubbles beneath the membrane. If air bubbles or damage to the membrane is evident,

replace the membrane housing and solution. Ensure that membrane housing has been filled

with KCl solution for at least 1 hour before calibration and first use. Training videos for how to

calibrate the YSI 550A meter and monitor with it are available on the WAV website

(http://watermonitoring.uwex.edu/wav/monitoring/video.html). Please contact your local

coordinator or the WAV coordinator if you have any questions!

Water Action Volunteers –Level 2 Manual Updated January 2016

YSI 550a DO Meter Calibration Procedure

*Before calibrating your meter at the beginning of the field season, please fill out the top

portion of the calibration log with the following information:

Your name as well as that of any other team members

Your organization name

The serial number for your meter (located on the back of the meter)

**The numbers along the top of the calibration log correspond to the steps in the protocol.

1. Turn on the meter and allow at least 15 minutes of warm up time before calibration.

Be sure to note the time that you turned on the meter as this information will be

important during the calibration process.

2. Verify that the sensor membrane is in good condition by removing the sensor from the

calibration/storage chamber and doing the following:

Check that the sponge is damp (rewet if necessary).

Shake or blow off excess water on the sensor.

Check for holes or tears in the membrane.

Check for air bubbles beneath the membrane.

3. Reinsert the sensor into the calibration/storage chamber at the back of the meter.

4. On the calibration log, record the date, time,

name of analyst (who is calibrating the meter),

and the altitude (in feet) at which the

calibration is taking place.

You can find your local altitude by using

a Wisconsin Atlas and Gazeteer or a

USGS topographic map.

i. Elevations may appear in meters, but you can convert meters to feet by

multiplying the meter value by 3.28.

5. Record the number of minutes of warm up time on the DO calibration log.

6. Record the stabilized probe temperature (°C) on

the DO calibration log (red box).

7. Record the stabilized pre-calibration D.O. (mg/L) on

the DO calibration log (orange box).

Press the MODE button to change from %

saturation to mg/L.

Water Action Volunteers –Level 2 Manual Updated January 2016

8. Press and release both the UP ARROW and DOWN ARROW keys

at the same time to enter the calibration menu.

The meter will say CAL on the screen in large letters and

CAL in small letters in the lower left corner. This will stay

on the screen until the calibration process is done.

9. Press ENTER on the meter to show a value for altitude (x100) in feet.

For example, enter a ‘9’

here indicates 900 ft and a

‘12’indicates 1200 ft.

10. Adjust the altitude with the up and

down arrow buttons for the

elevation where the calibration is

taking place and press ENTER.

The % saturation value is now showing on the meter display.

11. Allow the % saturation value (red box) to stabilize on the screen

This may take a minute or two to happen

12. Press ENTER (orange box).

The salinity of the water samples is now on the screen.

13. Press ENTER again to accept 0 (the salinity of fresh water).

The calibrated % saturation value is now on the screen

(red box) and the small CAL is no longer visible in the

lower left corner.

14. Press the MODE key (orange box) to switch to mg/L. (This key is

used to toggle between mg/L and % saturation.)

Water Action Volunteers –Level 2 Manual Updated January 2016

15. Record the post-calibration D.O. (mg/L) on the calibration log.

16. Look up the calibration chart D.O. value (mg/L) from the table

(available on the next two pages of this manual and as a

laminated sheet in the monitoring kit).

To read the table, find the probe temperature (y-axis)

and the altitude (x-axis) and record the corresponding

D.O. (mg/L) value as the calibration chart D.O. on the

calibration log.

If the difference between the post-calibration D.O. and the calibration chart D.O.

is greater than 0.3 (mg/L), re-calibrate the meter before using in the field.

17. Record any comments about the calibration process in the Comments field on the log.

18. Conduct a post-calibration drift test to verify accuracy of your calibration

If unsuccessful, check on the condition of the probe tip and contact the WAV

coordinator for further instructions.

**Post Calibration Drift

This quality control step must be performed to insure the collection of accurate data. After

calibration, check the meter for drift. This is a check as to how well the meter is holding the

calibration. Leave the meter where you calibrated it (this step is important to insure a stabilized

temperature) and walk away for 5 minutes. Upon returning, the DO percent saturation value

should be within a couple of percent of the calibrated percent saturation value you recorded. If

it has drifted more than a couple of percent, you must remove the cap membrane to check the

condition of the probe. If necessary, refurbish the probe tip according to your instrument’s

manual, and change the solution and membrane (contact the WAV coordinator for further

instructions. Once the refurbishment process is completed, wait one hour before repeating the

calibration procedures listed above (Steps 1-18).

19. After completing a successful calibration and post-calibration drift test, the DO meter

should be left on until the last reading of the day is completed.

20. Once monitoring is complete, moisten the sponge in the calibration chamber and turn

the meter off before storing it in the monitoring kit until the next field event.

Note: If you notice your meter is not calibrating properly, please check for bubbles or tears in

the membrane and replace if necessary. If replacing the membrane does not work, try cleaning

the probe by following instructions in the instrument’s manual (located in the pocket of your

meter case) or contact the WAV coordinator for further instructions or to inquire about a

replacement new meter.

Dissovled Oxygen Saturation (mg/L) Based on Elevation or Ambient Barometric Pressure (Station Pressure)

Elv: 2000 1900 1800 1700 1600 1500 1400 1300 1200 1100 1000 900 800 700 600 500 400 300 200 100 0* -200

Press: 706.5 709.1 711.8 714.5 717.1 719.8 722.5 725.1 727.8 730.5 733.1 735.8 738.5 741.1 743.8 746.5 749.1 751.8 754.5 757.1 760 765.1

032.0 13.6 13.6 13.7 13.7 13.8 13.8 13.9 14.0 14.0 14.1 14.1 14.2 14.2 14.3 14.3 14.4 14.4 14.5 14.5 14.6 14.62 14.7

133.8 13.2 13.3 13.3 13.4 13.4 13.5 13.5 13.6 13.6 13.7 13.7 13.8 13.8 13.9 13.9 14.0 14.0 14.1 14.1 14.2 14.22 14.3

235.6 12.9 12.9 13.0 13.0 13.0 13.1 13.1 13.2 13.2 13.3 13.3 13.4 13.4 13.5 13.5 13.6 13.6 13.7 13.7 13.8 13.83 13.9

337.4 12.5 12.6 12.6 12.7 12.7 12.7 12.8 12.8 12.9 12.9 13.0 13.0 13.1 13.1 13.2 13.2 13.3 13.3 13.4 13.4 13.46 13.6

439.2 12.2 12.2 12.3 12.3 12.4 12.4 12.5 12.5 12.6 12.6 12.6 12.7 12.7 12.8 12.8 12.9 12.9 13.0 13.0 13.4 13.11 13.2

541.0 11.9 11.9 12.0 12.0 12.0 12.1 12.1 12.2 12.2 12.3 12.3 12.4 12.4 12.5 12.5 12.5 12.6 12.6 12.7 12.7 12.77 12.9

642.8 11.6 11.6 11.7 11.7 11.7 11.8 11.8 11.9 11.9 12.0 12.0 12.1 12.1 12.1 12.2 12.2 12.3 12.3 12.4 12.4 12.45 12.5

744.6 11.3 11.3 11.4 11.4 11.5 11.5 11.5 11.6 11.6 11.7 11.7 11.8 11.8 11.8 11.9 11.9 12.0 12.0 12.1 12.1 12.14 12.2

846.4 11.0 11.0 11.1 11.1 11.2 11.2 11.3 11.3 11.3 11.4 11.4 11.5 11.5 11.5 11.6 11.6 11.7 11.7 11.8 11.8 11.84 11.9

948.2 10.7 10.8 10.8 10.9 10.9 10.9 11.0 11.0 11.1 11.1 11.2 11.2 11.2 11.3 11.3 11.4 11.4 11.4 11.5 11.5 11.56 11.6

10 50.0 10.5 10.5 10.6 10.6 10.7 10.7 10.7 10.8 10.8 10.8 10.9 10.9 11.0 11.0 11.0 11.1 11.1 11.2 11.2 11.2 11.29 11.4

11 51.8 10.3 10.3 10.3 10.4 10.4 10.4 10.5 10.5 10.6 10.6 10.6 10.7 10.7 10.8 10.8 10.8 10.9 10.9 10.9 11.0 11.03 11.1

12 53.6 10.0 10.1 10.1 10.1 10.2 10.2 10.2 10.3 10.3 10.4 10.4 10.4 10.5 10.5 10.5 10.6 10.6 10.7 10.7 10.7 10.78 10.8

13 55.4 9.8 9.8 9.9 9.9 9.9 10.0 10.0 10.1 10.1 10.1 10.2 10.2 10.2 10.3 10.3 10.3 10.4 10.4 10.5 10.5 10.54 10.6

14 57.2 6.6 9.6 9.7 9.7 9.7 9.8 9.8 9.8 9.9 9.9 9.9 10 10.0 10.0 10.1 10.1 10.2 10.2 10.2 10.3 10.31 10.4

15 59.0 9.4 9.4 9.4 9.5 9.5 9.6 9.6 9.6 9.7 9.7 9.7 9.8 9.8 9.8 9.9 9.9 9.9 10.0 10.0 10.0 10.08 10.2

16 60.8 9.2 9.2 9.2 9.3 9.3 9.3 9.4 9.4 9.5 9.5 9.5 9.6 9.6 9.6 9.7 9.7 9.7 9.8 9.8 9.8 9.87 9.9

17 62.6 9.0 9.0 9.1 9.1 9.1 9.2 9.2 9.2 9.3 9.3 9.3 9.4 9.4 9.4 9.5 9.5 9.5 9.6 9.6 9.6 9.66 9.7

18 64.4 8.8 8.8 8.9 8.9 8.9 9.0 9.0 9.0 9.1 9.1 9.1 9.2 9.2 9.2 9.3 9.3 9.3 9.4 9.4 9.4 9.47 9.5

19 66.2 8.6 8.7 8.7 8.7 8.8 8.8 8.8 8.9 8.9 8.9 8.9 9.0 9.0 9.0 9.1 9.1 9.1 9.2 9.2 9.2 9.28 9.3

20 68.0 8.5 8.5 8.5 8.5 8.6 8.6 8.6 8.7 8.7 8.7 8.8 8.8 8.8 8.9 8.9 8.9 9.0 9.0 9.0 9.1 9.09 9.2

Temp

Elevation Feet Above Sea Level/Equivalent Un-Corrected1 Barometric Pressure mm Hg

Dissovled Oxygen Saturation (mg/L) Based on Elevation or Ambient Barometric Pressure (Station Pressure)

Elv: 2000 1900 1800 1700 1600 1500 1400 1300 1200 1100 1000 900 800 700 600 500 400 300 200 100 0* -200

Press: 706.5 709.1 711.8 714.5 717.1 719.8 722.5 725.1 727.8 730.5 733.1 735.8 738.5 741.1 743.8 746.5 749.1 751.8 754.5 757.1 760 765.1

20 68.0 8.5 8.5 8.5 8.5 8.6 8.6 8.6 8.7 8.7 8.7 8.8 8.8 8.8 8.9 8.9 8.9 9.0 9.0 9.0 9.1 9.09 9.2

21 69.8 8.3 8.3 8.3 8.4 8.4 8.4 8.5 8.5 8.5 8.6 8.6 8.6 8.7 8.7 8.7 8.8 8.8 8.8 8.8 8.9 8.91 9.0

22 71.6 8.1 8.2 8.2 8.2 8.2 8.3 8.3 8.3 8.4 8.4 8.4 8.5 8.5 8.5 8.6 8.6 8.6 8.6 8.7 8.7 8.74 8.8

23 73.4 8.0 8.0 8.0 8.1 8.1 8.1 8.2 8.2 8.2 8.2 8.3 8.3 8.3 8.4 8.4 8.4 8.5 8.5 8.5 8.5 8.58 8.6

24 75.2 7.8 7.9 7.9 7.9 7.9 8.0 8.0 8.0 8.1 8.1 8.1 8.1 8.2 8.2 8.2 8.3 8.3 8.3 8.4 8.4 8.42 8.5

25 77.0 7.7 7.7 7.7 7.8 7.8 7.8 7.9 7.9 7.9 7.9 8.0 8.0 8.0 8.1 8.1 8.1 8.1 8.2 8.2 8.2 8.26 8.3

26 78.8 7.5 7.6 7.6 7.6 7.7 7.7 7.7 7.7 7.8 7.8 7.8 7.9 7.9 7.9 7.9 8.0 8.0 8.0 8.1 8.1 8.11 8.2

27 80.6 7.4 7.4 7.5 7.5 7.5 7.5 7.6 7.6 7.6 7.7 7.7 7.7 7.7 7.8 7.8 7.8 7.9 7.9 7.9 7.9 7.97 8.0

28 82.4 7.3 7.3 7.3 7.4 7.4 7.4 7.4 7.5 7.5 7.5 7.6 7.6 7.6 7.6 7.7 7.7 7.7 7.7 7.8 7.8 7.83 7.9

29 84.2 7.1 7.2 7.2 7.2 7.3 7.3 7.3 7.3 7.4 7.4 7.4 7.4 7.5 7.5 7.5 7.6 7.6 7.6 7.6 7.7 7.69 7.7

30 86.0 7.0 7.1 7.1 7.1 7.1 7.2 7.2 7.2 7.2 7.3 7.3 7.3 7.3 7.4 7.4 7.4 7.5 7.5 7.5 7.5 7.56 7.6

31 87.8 6.9 6.9 7.0 7.0 7.0 7.0 7.1 7.1 7.1 7.1 7.2 7.2 7.2 7.2 7.3 7.3 7.3 7.3 7.4 7.4 7.43 7.5

32 89.6 6.8 6.8 6.8 6.9 6.9 6.9 6.9 7.0 7.0 7.0 7.0 7.1 7.1 7.1 7.1 7.2 7.2 7.2 7.3 7.3 7.30 7.4

33 91.4 6.7 6.7 6.7 6.8 6.8 6.8 6.8 6.9 6.9 6.9 6.9 7.0 7.0 7.0 7.0 7.1 7.1 7.1 7.1 7.2 7.18 7.2

34 93.2 6.6 6.6 6.6 6.6 6.7 6.7 6.7 6.7 6.8 6.8 6.8 6.8 6.9 6.9 6.9 6.9 7.0 7.0 7.0 7.0 7.06 7.1

35 95.0 6.5 6.5 6.5 6.5 6.6 6.6 6.6 6.6 6.7 6.7 6.7 6.7 6.8 6.8 6.8 6.8 6.8 6.9 6.9 6.9 6.95 7.0

Source: J. Sullivan, WDNR-LaCrosse, March 2006

1Dissolved oxygen saturation values based on elevation due not account for TRUE station pressure. For more accurate dissolved oxygen saturation values use un-corrected pressure measurements.

Uncorrected barometric pressure values = National Weather Service Barometric Pressure in mm Hg x (Equivalent elevation pressure/760)

Temp

Elevation Feet Above Sea Level/Equivalent Un-Corrected1 Barometric Pressure mm Hg

*Dissolved oxygen saturation at 760 mm Hg derived from the tables of Benson & Kuause (1980), from C.M. Mortimer 1981. The oxygen content of air-saturated fresh waters over ranges of temperature

and atmospheric pressure of limnological interest. Mitt. Int. Ver. Limnol. No. 22. Stuttgart, Germany.

Water Action Volunteers –Level 2 Manual Updated January 2016

YSI 550a DO Meter Monitoring Procedure

Before going out to your stream site to monitor dissolved oxygen (or any parameter), please

remember to fill out the top portion of the monitoring datasheet with your name (s), the

station name, and the station ID #, as well as the date and time of the monitoring event.

It is also important that you indicate what type of equipment was used to monitor for dissolved

oxygen. In this case, you will circle the YSI 550A Meter option on your datasheet.

To measure dissolved oxygen (DO) in the stream:

1. With the DO meter in hand, enter the stream downstream from your monitoring

location (as to not disturb the bottom sediment as you move towards the site).

a. You can also monitor dissolved oxygen from a bridge overpass as to not disturb

the bottom sediment.

2. Remove the probe from its chamber and insert it into the water to be measured.

a. Continuously stir or move the probe through the water (especially when

monitoring in very still water) while taking the measurement.

3. Allow the temperature and dissolved oxygen readings to stabilize.

a. This should take anywhere from 2 to 5 minutes.

4. Observe and record the water temp and the DO values on the monitoring datasheet.

5. Press the MODE button to change the instrument display.

6. Observe and record the % saturation value on the monitoring datasheet.

7. Rinse the probe with distilled water after each use, and return it to the storage

chamber.

8. Before storing the meter in the carrying case, moisten the probe sponge by soaking with

distilled water.

9. Press the green button on the meter to turn if off at the conclusion of the day’s

monitoring effort.

Water Action Volunteers –Level 2 Manual Updated January 2016

Oakton Acorn pH 5 and 5+ Meter

WAV volunteers currently use one of three different pH meters:

1. pH 5 (original software and original outside casing)

2. pH 5 (updated software, but original outside casing)

3. pH 5+ (updated software and updated outside casing)

pH 5 meter

pH 5+ meter

Which pH meter do I have?

Before calibrating your pH meter, you will first need to ascertain which meter version you are

using. This information is important to know because the calibration protocol is varies slightly

between the different software versions.

The only way to tell if you have meter version #1 or #2 is by going through the calibration

process and seeing what happens between the calibration with the pH 7 buffer solution

calibration and the calibration with the pH 10 buffer solution. The original software tells the

meter to exit calibration mode and return to measurement mode upon completion of the first

calibration point. This means that you will complete two ‘one point’ calibrations as you have to

re-enter calibration mode in order to complete the pH 10 calibration. The software update on

the newer meters keeps the meter in calibration mode throughout the calibration process (the

values on the screen will continue to blink). This means that you will complete a ‘two point’

calibration when calibrating the meter.

For all meters used by WAV volunteers…

Both the Oakton Acorn pH 5 and the 5+ meters need to be calibrated before being used in the

field each and every time you monitor throughout the field season. Once calibrated, the pH

meter can be turned on and off between monitoring events if you monitor at multiple locations

in one day (unlike the DO meter).

When calibrating your meter, please use both the pH 7 and pH 10 buffer solutions (NIST)

provided in order to calibrate the meter. Do not reuse the buffer solutions following the

calibration as contaminants in the solution can impact the accuracy of the calibration and the

field measurements.

Water Action Volunteers –Level 2 Manual Updated January 2016

In order to avoid damaging the meter, be sure to keep the pH electrode (specifically the bulb at

the end of the probe) wet when not in use. Store the electrode in the electrode soaker bottle

and keep it filled with electrode storage solution. Refresher storage solution can be found in

your monitoring kit. Contact the WAV coordinator if you need more solution.

If you notice your meter is not calibrating properly, try cleaning the probe by following the

instructions in the instrument’s manual (located in the pocket of your meter case) or contact

the WAV coordinator for directions.

*Before calibrating your meter at the beginning of the field season, please fill out the top

portion of the calibration log with the following information:

Your name, as well as that of any other team members

The name of your organization

The serial number for your meter (located on the back of the meter)

The type of meter that you are using

oThis can be recorded after the first calibration is completed

Water Action Volunteers –Level 2 Manual Updated January 2016

Cal. procedure - pH 5 meter with original software and original outside casing

This calibration process will be completed by conducting two one-point calibrations. This means

that the meter will complete a single point calibration and then exit calibration mode each

time. You will have to restart the calibration process when using the pH 10 buffer solution by

pressing the CAL button.

1. Connect pH and temperature probes to meter.

a. Ensure that the pH probe connecter snaps into place.

2. Turn the meter on by pushing the On/Off button on the face of the meter.

3. Record the date, time, and the name of analyst

performing the calibration on the calibration log.

4. Remove the pH electrode from the electrode soaker

bottle.

5. Rinse the pH electrode with distilled water and shake the excess water off of the

electrode.

6. Click the MODE button to find the temperature

reading and record value on the calibration log.

7. Click the MODE button again to return to the pH screen on the meter.

8. Press the CAL button on the meter in order to start the calibration process (blinking).

9. Place the electrode and temperature sensors into the pH buffer solution and submerge

the glass bulb completely in the solution.

10. Wait for the reading to stabilize and record the

Stabilized pH value on the calibration log.

11. Press the ENTER key once and record the Calibrated

pH value (the meter is now out the calibration

mode and in measuring mode).

12. Check that the Calibrated pH value is within 0.2 of the pH buffer standard (7 or 10).

a. If the value is more than 0.2 higher or lower than its pH buffer value (compare

values in the grey columns, outlined in blue), recalibrate the meter (new line).

b. Record the new results on the next line of the pH meter calibration log.

13. Remove the electrode from the buffer solution (refill if necessary), rinse with distilled

water and shake off the excess water from the electrode.

14. To finish the calibration, repeat steps 6 through 12 for the pH 10 buffer solution.

15. Replace the pH probe in the electrode soaker bottle (refill if necessary), turn the meter

off, and head out to the field to complete your monitoring activities.

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