OPTO-EDU A15.2604 Manuel utilisateur

A15.26 04

Polarizing Microscope

To ensure the safety and obtain satisfactory performance, please study this instruction manual thoroughly

before

start

use

the

instrument.

- 1 -

ATTENTIONS

1. Disassembly only by the professionals.

The microscope has been adjusted before shipping, Unprofessional-person should

not disassemble and remove any other parts.

If you have any questions, please contact with manufacturer or local distributor.

2. Note the input voltage if identical with operating voltage of

instruction.

This instrument designed for wide input voltage (100V~240V,47~63HZ), applicable

to most area .But if the supply voltage exceeds this range, the instrument will be

seriously damaged.

3. Use in safe way, prevent burns and fire.

When the Instrument power in working, temperature of bulb and lamp house will rise

sharply to meet the heat balance, so pay attention to anti-hot logo in case of be burned.

Bulb should be replace after cool down .

Do not use alcohol, gasoline, paper and other combustibles near the instrument, to

prevent a fire! !

4. Notes on replacing the bulb.

The instrument used with 12V /20W imported halogen lamp of G4, replacement

should be used the same specifications of the bulb, or it may cause equipment

damage.

Before replacing the lamp, must turn off power switch, and unplug it in case of

electric shock.!

Please don’t put finger mark、dust or oil strain etc on the bulb when In

replacement.

5. Requirements for handling and using environment

Please cut off the power and unplug the power plug before moving. Be careful not to

crush your finger when placed.

This instrument is a precision instrument, and it should be handle with care, severe

shock can cause serious damage to the inner related parts.

The required available environment for using of the equipment:

Indoor temperature: 0 ℃ ~ 40 ℃ Maximum relative humidity: 85%

High temperature or high humidity may cause mildew, fog or dew of the optical

components, will result in the instrument not work.

6. For the protection of the environment, please properly handle the

microscope packing waste (such as: cardboard, foam, etc. )!

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CONTENTS

1. Purposes ........................................................................................................... 5

2. Performance ..................................................................................................... 5

3. Installation and use .......................................................................................... 6

3.1 Installation ...................................................................................................... 6

3.2 Operation ........................................................................................................ 7

3.2.1 Electric component ................................................................................. 7

3.2.2 Place sample ........................................................................................... 7

3.2.3 Condenser ................................................................................................ 7

3.2.4 Focusing ................................................................................................... 7

3.2.5 Polarizing observation ............................................................................ 7

3.2.6 Use of tension adjusting handle............................................................ 9

3.2.7 Notice after operation ............................................................................. 9

4. Maintenance ....................................................................................................... 9

4.1 Clean ............................................................................................................... 9

4.2 Enviroment for use ........................................................................................ 9

4.3 Replacement of bulb ................................................................................... 10

4.4 Replacement of fuse ................................................................................... 10

4.5 Not in use...................................................................................................... 10

5. Troubleshooting ............................................................................................... 11

- 3 -

Fig.1 B-POL Polarizing Microscope Size -A

01—Monocular tube 02—λ Slip , λ/4 Slip

03—Nosepiece 04—Stage

05—Condenser 06—Filter（removable）

07—Collector 08—Base

09—Condenser lifting handle 10—Left Fine focusing knobs

11—Left Coarse focusing knobs 12—Clamps

13—Body 14—Midbody

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Fig.2 B-POL Polarizing Microscope Size –B

15—Eyepiece 16—Observing tube locking screw

17—Analyzer change-over handle 18—Vernier

19—Stage locking screw 20—Aperture diaphragm handle

21—Polarizer unit 22—Potentiometer knob

23—Right Coarse focusing knobs 24—Right fine focusing knobs

25—Tension adjusting handle 26—Locking screw for midbody

27—Switch slip for Bertrand Lens

- 5 -

1. Purposes

This transmitting polarizing microscope is designed on the basis of biological

microscope ,use the polarization of light to make microscopic observation for double

refraction material. It is widely used in geology, chemical industry ,metallurgy,

semiconductor, petroleum, spinning field etc, also play important role in biological,

phytology, pharmacology, pathology field etc.

2. Performance Parameter

2.1. Total Magnification

Objective

Eyepiece 4× 10×

40× 20×

（optional）

60×

（optional）

10×/φ18(Reticule) 40× 100×

400× 200× 600×

10×/φ13(Optional) 40× 100×

400× 200× 600×

2.2. Objective（with Eyepiece 10×/18）

Non-stress

Achromatic

Objective

Numerical

Aperture

N.A.

Objective

Field of View

Resolution

4× 0.10 4.5mm 3.36μm

10× 0.25 1.8mm 1.34μm

40×(s) 0.65 0.45mm 0.52μm

20×（optional） 0.40 0.9mm 0.84μm

60×(s)（optional） 0.80 0.3mm 0.42μm

2.3. Other Parameter

2.3.1. Mechanical Tube Length： 160mm

2.3.2. Conjugate distance： 195mm

2.3.3. Eyepiece viewing tube： Monocular head，Inclined 30°.

2.3.4. Midbody：

Analyzer can rotate 90°，with scale, can move out of the optical path for single

polarizing observation.

Bertrand lens can be move out of the optical path

Standard equipped λ and λ/4 ，quartz wedge is optional.

2.3.5.Nosepiece： Quadplex hole inner location.

- 6 -

2.3.6.Rotatable stage：

Diameter Φ142mm，360° uniform scale ，division 1°,vernier division 6′.

With high precision bearing construction , rotatable stage has been reset before

factory ,polarizing attached mechanical stage is optional .

2.3.7. Focusing unit:

Coaxial Coarse And Fine Focusing Knobs, Coarse stroke 22mm, up position is

optional；Fine division 2µm, Condenser up-down range 25mm.

2.3.8. Condenser：

Abbe Condenser N.A.1.25 , Adjustable Aperture Diaphragm , Aperture Center

Can Be Adjustable ,

2.3.9. Polarizer:

Installed In The Bottom Of The Condenser ,Can Rotate Out Of The Optical

Path.

2.3.10.Filter: Located Upon The Collector, Blue Is Standard ,Can Be Removed.

2.3.11.Antifungus Device:

Objective, Eyepiece And Head All Take Antifungus Measures.

2.3.12 Electric components:

Input voltage AC100-240V, 47~63Hz

Output voltage DC3V～12V

Halogen lamp 12V/20W(LED lamp is optional)

Potentiometer with switch rotate knob.

Fuse 5A φ5×20

3. Installation and use

3.1 Installation

3.1.1 Take out main body and related parts，accessory ,tool, dust cover etc ,place carefully on

the working desk.

3.1.2 Make sure all items taken out before deposal packing case.

3.1.3 Loose the locking screw with socket head wrench(Fig.2-26),detach the monocular head

from main body.

3.1.4 Install midbody in the hole which on the upper end of main body’s arm( note the location

direction),and locking the screw(Fig.2-26).

3.1.5Install monocular head in the upper port of midbody, locking the screw for observing tube

(Fig.2-16) with small wrench.

3.1.6 Insert eyepiece in the tube .

3.1.7Please install objectives into the nosepiece in proper order according to magnification

power（switch to right place）.

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3.1.8 Please connect main body with power line if in detachable power supply.

▲Make sure all items taken out before deposal packing case.

▲Nosepiece should be rotate by wrenching the outer gear rim.

▲handle gently during installation, especially don’t touch lens.

▲For the protection of the environment, please properly handle the microscope packing

waste.

3.2 Operation

3.2.1 Electric component

Make sure the power supply voltage in input voltage range,then plug in socket ,if not will

cause damge for instrument.The microscope has rotatable potentiometer with switch ,it will

prolong bulb’s service life in case of high electricity current.

3.2.2.Place sample.

Please put cover glass upward on the stage , hold with clamps.

Placing sample should be carefully in case of damage. The sample should be put in optical path

by vertically and horizontally moving handle.

3.2.3 Condenser (see Fig.1-2)

a. Lift

Adjusting the distance between condenser and sample by rotating condenser lifting

handle ,can change the light’s degree of uniformity for best brightness.

b. Adjustment of Aperture Diaphragm

Adjust the size of aperture diaphragm by sliding the aperture diaphragm handle to

change sample’s contrast.

Normally, make the image full of 70%-80% of objective exit pupil diameter will get

satisfactory contrast grade .

3.2.4 Focusing

Because 10X objective has big field of view, long depth of field, can find image plane easily,

so usually making focus with it.

a. Put 10X objective in optical path for general outline ,then rotate fine focusing knob slowly

until becoming distinct for satisfactory image.

b. All equipped objectives have been adjusted through accurate focus, image should be distinct

with other objective after adjusting focus with 10Xobjective, If not, can rotate the fine focusing

knobs until getting satisfactory image.

3.2.5 Polarizing observation

a. Confirm the polarizer which under condenser has been moved in the optical path. It is in

horizontal direction ,has been adjusted in manufacture.

- 8 -

b. Put the up analyzer in good position and rotate to 0°,then analyzer and polarizer should in

orthogonal position, field will become more darker from objective, otherwise should adjust the

analyzer.

You can determine mineral slice sample’s interference colors order, birefringence ,extinction

type, extinction angle, elongation sign etc through orthogonal polarizing angle , Also can

judge whether it’s crystal twin mineral.

c. Switch analyzer to 90°, the analyzer and polarizer in same direction, the polarizing light can

transmit completely if no sample on stage and can observe in bright field. If sample slice has

anisotropic material

(besides vertical optical axis ) also can observe interference color for complementary color, it is

different from orthogonal interference color.

d. Putting the polarizer out of optical path can achieve single polarizing observation.

Single polarizing light can observe and determine mineral’s outside characteristic(shape,

cleavage), optical properties of light absorption(color,diversity),optical properties related with

refractive index(processus, exasperate, edge, baker red、dispersion etc.)

e. Angular deflection of analyzer can enhance contrast when in

bright field.

f. According to sample’s interference pattern to choose suitable compensation slip(λ , λ/4,

quartz wedge) ,then insert the midbody slot, mark side is upward ,location notch is

backward(left is λ, right is λ/4,middle for normal observation). quartz wedge also in the same

notch.

λ( gypsum、first class red)slip can show first class purplish red in orthogonal polarizing light,

inserting optical path will higher or lower

the interference color lever, is appropriate for mineral with more lower interference color(below

second yellow).

λ/4(mica) can show first class grey interference color ，inserting optical path will rise , drop

one interference color lever according to color spectrum ，is appropriate for mine with more

higher interference color .

Insert quartz wedge in the optical path will appear interference color from one class to four

class from thin to thick in orthogonal interference condition.

g. Putting Bertrand lens shift slip in the optical path can make conoscopic observation.

- 9 -

Conoscopic observation usually with high power objective and can clearly see the amplified

interference pattern, confirm its axis and slice direction, determine mineral’s light symbol and

axis angle (biaxial crystal）.

▲Don’t reverse the direction forcefully for left and right coarse & fine focusing knobs at

the same time ,otherwise will damage focusing unit.

▲Transform eyepiece with different power ，don’t direct wrench objective to rotate

nosepiece. should rotate the nosepiece by holding the insection of nosepiece cover to make

objective in right position for optical path，otherwise will cause effect for optical

performance.

3.2.6 Use of tension adjusting handle

Stage will slide down automatically after in long term usage.

Tightness adjusting ring can adjust coarse focusing unit in case of this conditions and make

adjusting operation comfortable.

Clockwise rotation is loose, in contrast ,is locked.

3.2.7 Notice after operation.

a. Please cut off power (rotate potentiometer knob )when not in use, unplug the power socket.

If use immersion oil ,should wipe off the lens and slices , put on dust cover.

b. If not in use for long time, suggest to place eyepiece 、objective in the dry containers (such

as moisture-proof cylinder), and place desiccant. Put on dust cover for main body.

4. Maintenance

4.1 Clean

4.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft brush or absorbent

cotton or cleaned by absorbent cotton, lens paper with the mixture of alcohol and ether

(proportion 1:4).

4.1.2 Alcohol and ether all are burnt easily, please take them away from fire. Be careful for turn

on and off power.

4.1.3 Don’t clean painted metal and galvanizing metal with organic solvent such as alcohol,

ether or the mixture of the both. Silicon cloth or soft cleaning preparation is suggested to clean

it.

4.1.4 Plastic should be cleaned by soft cloth with clear water.

4.2 Enviroment for use

4.2.1 Microscope should be used and placed in a cool, dry, non-dust, non-shake and

non-corrosive gases environment.

4.2.2 Normal working condition：

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