Olympus BX53 Manuel utilisateur



A.S

1. Si

2. T

3. T

4. S



B.H

1. Li

2. S

3. H

4. P

WMicrosco

stemStart‐

gnintothel

rnon(orw

rnonthem

nonlamp



artcellSens

rdwareCon

htpathbar

lectanobje

logenlamp

htwillbevi

iorZ‐stage

osetotheo

ob;theexte

eselecteds

ttononthe

c

e

j

yLaborator

Oly

p.

gbookont

keup)thec

croscope

(onlyifyou

imension

rolandSett

directsli

tivelensby

dialknob

iblefromth

ontrolBox

jectiveand

ntofthefin

eedappear

controller.)

d



pus B

edesk(star

mputerand

themainco

eeditforflu

program.

ng.

httoeyepie

urningthen

ontrolsthe

condenserl

movesthe

OWNbutto

movement

ontheLED

53 Digi

/endtime).

select“USER

”

trolunit

,

orescencei

e,camera,

sepiecetur

rightnessof

ns.

tageupord

bringsitdo

anbeselect

creen(Note

f

al Micr

”

andentert

hePriorre

aging).

rboth.

byhan

transmissio

owntofocus

nquickly.

dHigh(H),

DON’TUSE

c

Oly

scope

hepassword

otez‐stage

.

lightforbri

onspecime

inermovem

edium(M),

hefocuskn

h



i

mpusB

User G

onthelog‐i

ontroller

,

htfieldview

.TheUPbu

ntofstage

orlow(L)by

bonthemi

c

53User

ide

window.

DP26colorc

ing.Thetra

tonbringst

anbecontr

pressing“SP

roscopean

g

Guide|

mera

,an

smission

estageup

lledwiththe

EED”button.

theZERO



5. R



C.O

WMicrosco

motecontr

ttonstosel

enorclose

erviewofc

henthepr

istochemica

indowtab

electanobj

tagewilllow

atchingobj

bjectivetoo

lickoneof

ocusontoth

anel.

1)

1!

yLaborator

llerbox

c

ctanappro

hemercury

llSenseprog

gramopens,

lystainedsa

.

ctive(start

erandames

ctiveinplac

btaincorrect

esampleby



ntrolsthes

riatefilterm

rclampligh

amandbas

firstsetupt

plesorflu

ith5xor10

sagewindo

.ClickOKo

scaleba

).

butto

movingstag

lectionofi

irrorcubefo

shutter.

cimageacq

eprogramf

rescenceim

objective)b

willpopup.

nthemessa

sontheOb

withPriorz

‐

agingmode

(

differentco

isition.

oryourimag

gingforfluo

pressingan

Turntheob

ewindow(I

ervationMe

‐

stagecontr

Oly

(i.e.BForFlu

lorsoffluore

ingneeds(i.

rescentlylab

objective

ectivenose

iscriticalto

thodspanel

llerorbycli

mpusB

orescence(F

scence.Pre

.bright‐field

ledsamples

ieceturret

atchthem

orbrightfiel

kingarrowh

53User

L)).Usethe

stheFLShu

imagingfor

).Clickthe

byhandto

gnification

orfluoresc

adsintheD

Guide|

irror

terbuttont

cquisition



button.Th

utthe

iththe

nceviewing.

viceUnits



(



D.B

(



WMicrosco

electacam

rightfield.

utomaticfr

electResolu

(

active)scen

rightfield(o

akesureth

heupperlig

lickLive

Automatice

orkfinefor

hentheset

s.vsior.tiff

yLaborator

rafromthe

electManu

mthewhite

ion

asde

.)

DIC)Image

tDP26cam

tpathbar

button

posure

w

mostcases).

ingissatisfa

rmatbyclic



ameralist

)

lorAutoma

balancemo

ired.(Note:

Acquisition

raisONand

ispushedin

andobtain

ithSpot30%

Clickthebu

ctory,clickS

kingtherigh

)

:XM10mon

ticexposure

elist.

oucanchoo

selectedfro

andthelow

roperexpos

regionanda

tonagainto

ap

mousebutt

chromeca

toobtainbe

ealowerre

thecamer

rbar1

is

reandwhit

utomaticw

stoplivevie

buttonands

noverthei

Oly

eraforfluo

terimages.

olutionforL

listandtha

ulledout.

balance

itebalance

ing.

avetheima

agetab.

mpusB

escenceor

ithDP26c

ive/Movie

t



e

53User

P26colorca

meraselect

rfas

updat

1@

1#

Guide|

erafor

d,select

oflive



E.Fl

1.

2.

one

posi

and

WMicrosco

uorescenceI

licktheFLb

noroffbyp

electanapp

ftheObser

ion

.Mak

helowerba

d

yLaborator

mageAcqui

tton

ont

ressingFLSh

ropriatefluo

ationMeth

surethatth

pushedin)



ition

eremotec

utterbutton



escencemir

dsbuttons

lightpath

ndXM10ca

ntrollerbox

onthere

orcubebyp

ars

arein

eraisselec

ousemercu

otebox.

ressingMirr

(exce

correctposi

ed.

Oly

ryarclampl

rbuttons

tBFandDIC

ion(theupp

e

mpusB

ghtforfluor

inthecontr

).Theselect

rbaratthe

53User

scence.Th

llerboxorcl

edmirrorwil

midorexten

f

Guide|

lightcanbe



ickingany

lbein

dedposition

b



3.L

and



4. Cl



5. Cl



6. I



7. S



WMicrosco

t’stakemul

xRedfluoro

icktheProc

ulticolorwh

elist.Repe

xttotheca

tton.IfAut

posuretime

nditionsfor

icktheFITC

timalexpos

annel.Once

reachchan

.vsifileint

vetheorigi

agescanals

changethe

oneimage

vetheimag

alebarcan

splayedwith

age.Savet

yLaborator

icolorfluore

hores.

ssManager

el butto

ttwicetose

eraicon;it

maticexpos

.Oncethee

DAPI.

ectiononth

retimeand

allthesettin

elautomatic

eLibraries>

alimagefile

obesaveda

mageinto8

‐

ontainingm

asmultiple

eviewedwi

View/InfoS

eannotated



cenceimag

andch

.Clickthe

ectFITC(gre

illexpandt

ure(withSp

posurecon

listanduse

clickico

sareobtain

ally.Theres

icturesfold

as.vsiform

.tifffiles.S

‐

bitimage.U

ltiplechann

magesfore

hView/

cal

ampcomm

imageasJP

acquisition

ecktheAuto

en)andTxRe

showcurre

t30%)isus

itionsarese

theAutoma

toreadthe

ed,clickthe

ltingmultic

rassoonas

twithouta

lectImage>

eFile>Save

ls.OruseFi

chchannel.

barcomma

nd.Thesea

G(JFIF)(*.jpg

ithanexam

maticProce

butto

d(red)chan

texposure

d,letthepr

,clickic

icexposure.

setting.Rep

but

annelimage

heacquisiti

ymanipulati

ode>8bit/

s…menuco

e>Exportto

ndandthe

notationsc

).

Oly

plestained

ses

.Click

andselect

els.Clickth

etting.Click

gramfindth

ontosetthe

Waituntilit

eatoncemo

on.Itwillta

,bydefault,

nhasbeenc

onorproces

hannelmen

mmandtos

tiffseries…

agnification

n’tbesaved

mpusB

ithDAPI,FI

the

API(blue)in

“plus”sign

theLive

eappropriat

seexposure

indsan

eforTxRED

kethreesho

illbesaved

ompleted.

ing.

ucommand

vetheimag

ommandto

infocanbe

withyourtif

53User

C,



s



Guide|

g



F.I

1. O



2. I



WMicrosco

ageProces

nceyousave

publication.

stogramand

default,the

mensionSel

teyesymb

wantedcha

yLaborator

ing

theoriginal

ClickthePr

thebrightne

adjustment

ectorpanel

lindicates“i

nels.Then,



ile,makea

cessingwin

ssandcontr

ftheHistog

,youcanch

visible”).Cl

youcanadju

uplicateifit

owtab

stofthedis

amortheDi

oosethecha

ckthecolor

stthehistog

snecessary

fro

laycanbea

splayEnhan

nnelstobev

hannelsyou

amandthe

Oly

omakeany

themainwi

justed.

ementappli

isible(eyesy

wanttosho

rightness/c

mpusB

hangesinth

ndowtabs,i

estoallthe

mbolind

andclicko

ntrastofth

53User

eimagefor

whichthei

isiblechann

cates“visibl

ntheeyesy

selectedch

Guide|

resentation

age

ls.Onthe

”andgrey‐

bolstohide

nnelonly.



3. 



(



WMicrosco

osavesome

erationswil

1) Makeac

Edit/Pas



2) Makeac

Thiswill



3) Selectall

multicha

selectch

c

yLaborator

overlaidima

automatica

mpositeim

eAs/NewI

mpositeim

reateanew

channelsfro

nelimagein

nnelstobe



esfromam

lychangeth

geasabove.

age.Thiso

geasabove.

magewiths

theDimen

oindividual

ombinedan

ultichanneli

imagetype

FromtheEd

erationwill

UseEdit/Co

alebarinpi

ionSelecto

channelima

clickOK.

ageyoujus

rom16bitg

itmenu,use

enerateaco

yDisplayto

elunit(losin

.UseImage

es.Thenus



Oly

madeasab

rayscaleto8

Edit/Select

mpositeima

Clipboar

a

gsomedime

Separate/C

Image/Co

mpusB

ve,therear

bitcolo

):

ll,thenEdit

ewiththes

dthenEdit

nsionalinfor

annelscom

bineChann

53User

ethreeways

Cop

,andt

mescaleba

PasteAs/Ne

mation).

andtosep

.Inthe

Guide|

(note:these

en

r.

Image.

ratethe

indow,



F.A

1. A

2. T

It

3. Cl



G.D

1. I

3. Y

4. Y

WMicrosco

quisitionof

z‐stackimag

ackbu

ovethestag

ocessMana

osetotheco

ingupthest

tton

,whi

sition.)

eprogram

jective.Clic

willcalculat

icktheStart

splayingan

theProcess

iththenavi

amesofaz‐s

ucanselect

ousebutton

amefromth

ucanalsos

ttons

;de

artingPositi

enchoose

icktheright‐

ntainingonl

coverslip

Slide

yLaborator

‐stack(z‐ser

containsm

tontodispla

usingtheP

erwindow.

verslipand

agetofocus

hwilltheSt

illautomati

kApplybutt

thenumber

buttontoca

Converting

ngtab,thez

‐

atorbar

tack.

asinglefra

inasinglefr

econtextm

lectashort

ermineane

nandchoo

leView.M

ousebutto

selectedfr



ies)

ltipleframe

Zstackpan

iorstageco

First,lower

henclickSet

ontoadeep

rtposition.

(

allyrecomm

ntoaccept

ofz‐slices



tureaz‐seri

‐stacks

‐

stackimage

efromaz‐st

meviewan

nu.

angeoffra

startingpo

eanewend

kesureallt

andselect

mewillbeg

Objectiv

acquiredat

el.Tosetth

trollerorby

hestagetof

button

,w

rregionclos

(

Imageacqui

ndastepsi

hisstepsize

accordingly.

s.Youcanc

canbemani

ackbyclicki

selectingE

eswithinth

itionwithin

positionand

echannelsa

xtrac

from

neratedas



differentfoc

StartandE

clickingthe

cusontoar

ichwillbet

totheslide

itionwillbe

e

forz‐se

orchangeas



mbinethis

ulatedinva

gtheright‐

tractCurren

stackbyusi

hestackan

clickSet

evisible(sel

hecontext

.vsifile.Sav

Oly

uspositions.

dlimitsofa

rrowheads

egionofinte

eEndpositi

andclickSe

inattheSta

iesaccordin

youwantan

unctionwith

iouswaysto



ngSetSelect

click

etSel

ctionEndP

ected).Over

enu.Ane

theimage.

End

Start

onstag

mpusB

Clickz‐

z‐stack,

inthe

rest

on.Next,



t



tothe

dapply.

multicolori

generatedi

,youc

ion

ction

sition.

animage,

z‐stack

g

53User

aging.Sav

ferentviews

nnavigated



f

Guide|

theimages.



ifferentfocal

b

c

d



5. Y







WMicrosco

ucanproje

omthemain

iththe

lice

ywhereby

iththeslice

mensionvie

yLaborator

tallthez‐st

menuorM

Viewmenu,

ovingthex

iewmenuo

ofextende



ckframesin

imumProj

oucandispl

cross‐hairlin

tions

x‐yslicepo

oasingle2

ctionOverZ

ayacross‐se

etothedesi

ition.

imagebyse

onthenavig

tionalz‐dim

edposition.

,suchas

Oly

ectingProce

torbarmen

ensionview



ExtendedSli

mpusB

ss/Projectio

u.

fanxandy

ce ,

53User

s/Maximu

plane.Youc

youcanvisu

Guide|

Zcommand

nview

alizez



Autres manuels pour BX53

Autres manuels Olympus Microscope

Olympus

Olympus BX41 Manuel utilisateur

Olympus

Olympus BHT Manuel utilisateur

Olympus

Olympus CKX31 Mode d'emploi

Olympus

Olympus BHTU-112 Manuel utilisateur

Olympus

Olympus VMF Mode d'emploi

Olympus

Olympus CHA-P Manuel utilisateur

Olympus

Olympus SZX10 Manuel utilisateur

Olympus

Olympus BX2 SERIES Manuel utilisateur

Olympus

Olympus BX53M Manuel utilisateur

Olympus

Olympus FH Manuel utilisateur

Olympus

Olympus BH2 Series Instructions d'installation

Olympus

Olympus X-Tr Mode d'emploi

Olympus

Olympus Pom Manuel utilisateur

Olympus

Olympus CX31 Manuel utilisateur

Olympus

Olympus Lext OLS3100 Manuel utilisateur

Olympus

Olympus CX40 Manuel utilisateur

Olympus

Olympus U-UVF248 Manuel utilisateur

Olympus

Olympus CH2 Series Mode d'emploi

Olympus

Olympus CH Series Mode d'emploi

Olympus

Olympus BX60 Manuel utilisateur

Olympus

Olympus MX61 Manuel utilisateur

Olympus

Olympus MIC-D Manuel utilisateur

Olympus

Olympus VS110 Fluorescence Manuel utilisateur

Olympus

Olympus BX51-P Manuel utilisateur

Manuels Microscope populaires d'autres marques

Thermo Scientific

Thermo Scientific Nicolet iN10 Mode d’emploi

National

National DC3-163 Manuel utilisateur

KERN Optics

KERN Optics OPE-1 Manuel utilisateur

OPTIKA MICROSCOPES

OPTIKA MICROSCOPES B-150D Manuel utilisateur

Prior

Prior Lux POL Manuel utilisateur

Opitec

Opitec Smartscoop 115615 Manuel utilisateur

KERN

KERN OPO-1 Manuel utilisateur

Topcon

Topcon SP-1P Manuel utilisateur

Motic

Motic PA43 Manuel utilisateur

Leica

Leica DVM5000 Guide de l'utilisateur

MshOt

MshOt MZ62 Manuel utilisateur

KERN Optics

KERN Optics OZR-5 Manuel utilisateur

VWR

VWR VisiScope 384 Series Manuel utilisateur

Nikon

Nikon ECLIPSE E200 POL Manuel utilisateur

Leica

Leica DI C800 Manuel

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manuel utilisateur

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manuel utilisateur

Leica

Leica FL560 Manuel utilisateur