Hanna Instruments HI84529 Manuel utilisateur

INSTRUCTION MANUAL

HI84529

Titratable Acidity

Mini Titrator & pH Meter

for Dairy Products

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the

Thank you for choosing a Hanna Instruments product.

Please read this instruction manual carefully before using this instrument.

This manual will provide you with the necessary information for correct use of this

instrument, as well as a precise idea of its versatility.

If you need additional technical information, do not hesitate to e‑mail us at

[email protected] or view our worldwide contact list at www.hannainst.com.

Dear

Customer,

TABLE OF CONTENTS

1. Preliminary Examination ..................................................................................................... 4

2. Specifications..................................................................................................................... 5

3. General Description & Intended Use ..................................................................................... 7

4. Principle of Operation ......................................................................................................... 9

5. Functional Description....................................................................................................... 10

6. Titrator Startup................................................................................................................. 13

7. Setup Menu ..................................................................................................................... 14

8. Guide to Display Codes...................................................................................................... 19

8.1. Pump Calibration Messages ..................................................................................... 20

8.2. pH Calibration Messages ......................................................................................... 21

8.3. Titration Messages .................................................................................................. 22

9. Electrode Preparation........................................................................................................ 23

10. Electrode Calibration Procedure........................................................................................ 25

11. pH Buffer Temperature Dependence.................................................................................. 29

12. Dosing Pump Installation ................................................................................................ 30

13. Dosing Pump Prime Procedure ......................................................................................... 31

14. Pump Calibration Procedure............................................................................................. 33

15. Titration Procedure.......................................................................................................... 36

15.1. Tips for an Accurate Measurement .......................................................................... 39

15.2. View / Delete Titrator Recorded Data....................................................................... 39

15.3. Titrator GLP Information......................................................................................... 41

16. pH Measurement............................................................................................................ 42

16.1. View / Delete Recorded pH Data............................................................................. 43

16.2. pH Meter GLP Information ..................................................................................... 44

17. PC Interface & Data Transfer............................................................................................ 45

18. Electrode Conditioning & Maintenance.............................................................................. 46

19. Troubleshooting Guide..................................................................................................... 47

20. Accessories..................................................................................................................... 49

Certification........................................................................................................................... 50

Recommendations for Users.................................................................................................... 50

Warranty .............................................................................................................................. 50

PrELImINAry ExAmINATION

1. PRELIMINARY EXAMINATION

Remove the instrument and accessories from the packaging and examine it carefully. For further

assistance, please contact your local Hanna Instruments Office or email us at [email protected].

Each HI84529 mini titrator is supplied with:

• FC260B pH electrode

• HI5315 Reference electrode

• HI7662-M Temperature probe

• HI84529-70 Reagent kit for titratable acidity in dairy products

• HI7072 Fill solution,30 mL

• HI700640 Cleaning solution for milk deposits, 20 mL sachet (2 pcs.)

• Capillary dropper pipette (1 pc.)

• 100 mL beaker (2 pcs.)

• Tube set (aspiration tube with titrant bottle cap and dispensing tube with tip)

• Dosing pump valve (1 pc.)

• 5 mL syringe (1 pc.)

• 1 mL syringe with tip (1 pc.)

• Stir bar (1 pc.)

• Power adapter

• Instrument quality certificate

• Instruction manual

Note: Save all packing material until you are sure that the instrument works correctly. Any

damaged or defective item must be returned in its original packing material with the supplied

accessories.

SPECIFICATIONS

2. SPECIFICATIONS

Titrator

Range

Low Range %I.a.: 0.01 to 0.20

°SH: 0.4 to 8.9

°D: 1.0 to 20.0

°TH: 1.1 to 22.2

High Range %I.a.: 0.1 to 2.0

°SH: 4.4 to 88.9

°D: 10 to 200

°TH: 11.1 to 222.2

Resolution

%I.a.: 0.01 (Low Range) / 0.1 (High Range)

°SH: 0.1

°D: 0.1 (Low Range) / 1 (High Range)

°TH: 0.1

Accuracy Low Range ± 0.01 %l.a. @ 25 °C

High Range ± 0.1 %l.a. @ 25 °C

Sample size

Low Range 20: 20 mL or 20 g

Low Range 50: 50 mL or 50 g

High Range 20: 20 mL or 20 g

Titration method Acid-base titration

Principle Endpoint titration

Pump speed 10 mL/min

Stirring speed 800 (Low Range) / 1000 (High Range)

Log data Up to 200 samples

pH Meter

Range -2.0 to 16.0 pH / -2.00 to 16.00 pH

Resolution 0.1 pH / 0.01 pH

Accuracy ± 0.01 pH

Calibration 1, 2 or 3 point calibration;

4 available buffers (4.01, 6.00, 8.30, 10.01)

Temperature

compensation Manual or automatic

mV Meter

Range -2000.0 to 2000.0 mV

Resolution 0.1 mV

Accuracy ± 1.0 mV

Log data Up to 200 samples (pH or mV)

SPECIFICATIONS

Temperature

Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)

Resolution 0.1 °C

Accuracy ±0.4 °C, without probe error

Electrode FC260B pH electrode

HI5315 Reference electrode

Temperature Probe HI7662-M

Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing

Power Supply 12 Vdc power adapter

Dimensions 235 x 200 x 150 mm (9.2 x 7.9 x 5.9”)

Weight 1.9 kg (67.0 oz.)

Required Reagents

HI84529-50 Titrant Low Range 20

HI84529-51 Titrant High Range 20

HI84529-52 Titrant Low Range 50

HI84529-55 Pump calibration standard

GENErAL DESCrIPTION & INTENDED USE

3. GENERAL DESCRIPTION & INTENDED USE

The HI84529 is an affordable, easy to use, microprocessor-based automatic mini titrator and pH

meter designed for the rapid and accurate analysis of Total Titratable Acidity in Dairy Products.

The HI84529 will quickly become a valuable tool by eliminating subjective factors including color

indicators, errors in calculations or erratic titrant additions.

The instrument benefits from Hanna Instruments’ many years of experience as a manufacturer of

quality analytical instrumentation. A clear and well-designed user interface makes the instrument

intuitive and simple to use.

By pressing the Start key in Titrator mode, the instrument will automatically titrate the sample to the

set endpoint, perform all necessary calculations and display the results in the selected unit. At the end

of the titration, another titration can be started by pressing the Restart key.

A dedicated HELP key aids in setup, calibration and troubleshooting.

Other features:

• pH meter / mV meter

• Stir speed control

• Graphic mode to display the titration data

• Data can be stored using the log feature and then exported to a USB stick or transferred to a PC

using the USB connection

• Log on demand for up to 400 samples (200 mV/pH measurements; 200 for titration results)

• GLP feature, to view calibration data for the pH electrode and pump

Significance of Use

Titratable acidity can be expressed in several units; % lactic acid (% l.a.), degree Soxhlet Henkel

(°SH), degree Dornic (°D) or degree Thörner (°TH). Each of these units corresponds to a specific

procedure used to titrate dairy products.

Soxhlet Henkel degrees (°SH)

mostly used in Central Europe exept France and the Netherlands.

This value is a number of mL of 0.25N NaOH used for titration of

100 mL milk, using phenolphtalein as indicator.

Thörner degrees (°Th)

mostly used in Sweden and the CIS. This value is obtained by

titrating 100 mL of milk, diluted with 2 parts of distilled water,

with 0.1N NaOH, using phenolphtalein as indicator

Dornic degrees (°D)

mostly used in Netherlands and France. This value is obtained

by titrating 100 mL of milk, with N/9 (0.11 N) NaOH, using

phenolphtalein as indicator.

Percent lactic acid (%l.a.)

frequently used in the UK, USA, Canada, Australia and New

Zealand This value is obtained in the same way as °D, dividing

the result by 100.

GENErAL DESCrIPTION & INTENDED USE

The titratable acidity values will vary depending on the method used. Select Low 50 to titrate a

non-diluted sample, or select Low 20 / High 20 to titrate 20 mL or 20 g samples that are diluted

with twice its volume of deionized a distilled water. The HI84529 uses methods based on AOAC

International and Standard Methods for the Examination of Dairy Products. Both of these methods

report titratable acidity as % lactic acid, a rough conversion factor can be used to convert the results

to the other available units:

From To Divide by

%l.a. °SH 0.0225

%l.a. °D 0.0100

%l.a. °TH 0.0090

The HI84529 can be customized to meet the needs of any dairy analysis lab. Samples can be titrated

by weight or volume, diluted or non-diluted (low range only) and titrated to a fixed pH endpoint that

can be adjusted by the user.

PrINCIPLE OF OPErATION

4. PRINCIPLE OF OPERATION

The methodology for titratable acidity in dairy products is based on neutralization reaction where

the acids in the sample (i.e. lactic acid) reacts with a base (i.e. sodium hydroxide) to produce water:

H++OH¯H2O

In an ideal solution, the endpoint of an acid titration corresponds stoichiometrically to the complete

neutralization of the acids present.

This endpoint for this reaction can be determined visually using a color indicator (i.e. phenolphthalein),

however this endpoint becomes very objective in opaque and colored samples. The HI84529 removes

this issue by titrating to a fixed pH endpoint that can be set by the user. The HI84529 can be

customized to meet individual needs with samples titrated by mass or by volume, in two different

ranges, with diluted and non-diluted (low range only). For precise analysis the sample size, volume

of titrant added and titrant concentration must be known.

The HI84529 Titratable Acid in Dairy Products Mini Titrator utilizes a simple sample preparation, a high

quality dosing pump for titrant additions, potentiometric endpoint determination and instantaneous

computations. To maintain the high accuracy of the mini titrator a simple pump calibration is required.

The pump calibration uses a known quantity of a known solution to compensate for changes in the

dosing system, this procedure should be performed regularly.

FUNCTIONAL DESCrIPTION

5. FUNCTIONAL DESCRIPTION

Front View

1. Dosing pump valve

2. Aspiration tube

3. Syringe

4. Titrant bottle

5. Dispensing tube

6. Reference electrode

7. Temperature probe

8. pH electrode

9. Electrode holder

Top View

2 3

1. Titrant bottle

2. Dispensing tube

3. Electrode holder

4. Liquid Crystal Display (LCD)

5. Functional keys

6. Keypad

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