Euromex OXION Series Manuel utilisateur
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euromex microscopen bv
The Netherlands
www.euromex.com
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Introduction
The Oxion series has been designed with all kind of ife Sciences applications and great durability
in mind. This resulted in a modern, robust and high level microscope for everyday use, equipped
with excellent optical and mechanical components. Specific attention to production methods
resulted also in an excellent price/performance ratio.
The Oxion features a unique swiveling concept allowing the viewing height to be easily matched
with the user height and compared to traditional microscopes add approximately 40 mm extra
viewing height. The Siedentopf tubes have standard HWF plan 10x eyepieces with 22 mm field of
view and adjustable diopter. The interpupillary distance is 50 to 75 mm. Eyepieces can be secured
to the tubes. The Oxion head has an ergonomic viewing angle of 30°. The Oxion is available in
various configurations suitable for ife Sciences applications in laboratories, high schools and
universities but also for routine medical applications and veterinary medicine. Besides the bright
field contrast method, configurations with phase contrast, dark field contrast, simple polarization
or fluorescence illumination can be used.
The computer optimized design of the Oxion ensures high stability and durability which results in
an advanced ergonomical all-round microscope. Below the head, the microscope features an
opening for insertion of an optional polarizer. A handgrip at the back of the stand allows easy
transportation of the microscope.
The large 150 x 140 mm table with 76 x 50 mm translation mechanical stage and a reversed
nosepiece for up to 5 objectives makes the Oxion suitable for advanced laboratory work.
Ergonomically placed adjustment knobs minimize fatigue during long microscopy sessions. The
general shape of the stand has been specifically designed to match today’s standard. The height
adjustable Abbe N.A. 1.25 condenser accepts sliders for economical phase contrast and dark field
applications.
The Oxion can also be equipped with a heating stage which can be set up to 50° Celsius by a real
PID controller. Higher temperatures can be achieved on special request. The 3 Watt adjustable ED
illumination offers the user a comfortable illumination with a very constant color temperature and
a very long term usage. The Koehler illumination enables highest contrast and maximum
achievable resolving power of the optics. Besides low energy consumption and no heating, the
high powered ED has a long operating lifetime. The 100 Watt mercury-vapor light source enables
a large range of wavelengths to be used for various fluorophores. The vertical light reflected
illuminator can accept up to 4 cubes at the same time for appropriate exciter and emitter filters
Bright field
A large scale of applications is covered by the common light-transmitted bright field contrast
method. Besides the standard plan achromatic 4x, 10x, 20x, S40x, S60x, S100x oil bright field, the
Oxion can also be engaged with newly designed high contrast plan semi-Apochromatic objectives.
The improved correction of these objectives results in lower aberrations and improved resolving
power for the complete optical system. All objectives are treated with a multi-layer anti-reflection
coating and are anti fungus.
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Phase contrast The observation of living cells and specimens with poor contrast is made possible
by the use of the phase contrast method. Two different phase contrast equipments are available.
All phase plan contrasts objectives can be used with both systems. A first economical way is to use
sliders, each equipped with phase contrast rings. The first slider features phase rings for 10x and
S40x and the second for 20x and S100x plan phase contrast objectives. These phase contrast
sliders are simply inserted into the standard condenser of the Oxion.
The second method is to use one of the 2 available Zernike phase contrast disc condensers. The
first Zernike disc condenser allows bright field contrast and phase contrast for 10x, 20x, S40x and
S100x phase plan objectives. The second one allows bright field and phase plan contrast for 10x,
S40x, S100x phase plan objectives and also features a dark field contrast method for
magnifications up to 400x. Both condensers are delivered with a telescope to adjust the phase
contrast rings.
Fluorescence Today, fluorescence microscopy offers a faster and reliable identification of
infectious diseases and allows observation of complex interactions in unstained living cells. The
high contrast plan semi-Apochromatic Fluarex 4x, 10x, 20x, S40x and S100x oil objectives
combined with the 22 mm eyepieces of the Oxion, offer the necessary resolution and field of view
for fast detection of small pathogens. A 100 Watt mercury –vapor light source combined with the
high performance fluorescence filters produce excellent contrasted images with an outstanding
signal-to-noise ratio.
Caution! In this manual a standard configuration is described. Many items are optional!
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Introduction .............................................................................................................................. 2
Construction of the microscope ................................................................................................. 6
Preparing the Oxion microscope for use ..................................................................................... 7
Handling ............................................................................................................................................................................................. 7
Assembling Steps ............................................................................................................................................................................... 8
Inserting/replacing the light source ................................................................................................................................................... 8
Mounting the objectives .................................................................................................................................................................... 8
The microscope head ......................................................................................................................................................................... 8
Placing the eye pieces ........................................................................................................................................................................ 8
The eyeshades ................................................................................................................................................................................... 8
Connecting the power cord ............................................................................................................................................................... 8
Operation: ................................................................................................................................. 9
Setting up the illumination ................................................................................................................................................................ 9
Place the specimen slide .................................................................................................................................................................... 9
Focusing and slide protection ............................................................................................................................................................ 9
Adjusting the focusing tension........................................................................................................................................................... 9
The interpupillary distance .............................................................................................................................................................. 10
The correct eye point ....................................................................................................................................................................... 10
People wearing glasses .................................................................................................................................................................... 10
Adjusting the diopter ....................................................................................................................................................................... 10
Abbe condenser ............................................................................................................................................................................... 11
The field (Köhler) diaphragm ........................................................................................................................................................... 11
Adjusting the Aperture Diaphragm .................................................................................................................................................. 11
Use of the S100x oil-immersion objective ....................................................................................................................................... 12
Using filters ...................................................................................................................................................................................... 12
continued on next page
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sing the Oxion range accessories ............................................................................................13
Using the Phase Contrast Slider ....................................................................................................................................................... 13
Mounting and operation of the Euromex Zernike phase contrast condenser set ........................................................................... 13
Using the Zernike phase contrast set. .............................................................................................................................................. 14
Centering the phase rings ................................................................................................................................................................ 14
Using the Dark Field Slider ............................................................................................................................................................... 14
Using the simple polarizing set ........................................................................................................................................................ 15
Using the 0.5x ½ Inch CS/C-mount camera adapter AE.5057 (for trinoculair models only) ............................................................ 15
Using the S R camera adapter AE.5527(for trinoculair models only) .............................................................................................. 15
Maintenance and cleaning ........................................................................................................16
Cleaning the optics .......................................................................................................................................................................... 16
Maintenance of the stand ................................................................................................................................................................ 16
Accessories ...............................................................................................................................17
Eyepieces and eyeshades ................................................................................................................................................................. 17
Objectives ........................................................................................................................................................................................ 17
Fluorescence .................................................................................................................................................................................... 17
Sliders and phase contrast sets ........................................................................................................................................................ 18
Miscellaneous .................................................................................................................................................................................. 18
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Construction of the microscope
The names of the several parts are listed below and are indicated in the picture:
A) Photo port N) Revolving nosepiece for 5 objectives
B) Microscope head O) Objectives
C) Set screw for tube P) Object clip
D) Stand arm Q) Object x/y stage
E) Stage controls R) Condenser with iris diaphragm
F) Coaxial fine adjustment S) Height adjustment condenser (not visible)
G) Coaxial coarse adjustment T) Köhler (field) diaphragm + filter holder
H) On/Off switch with light adjustment X) Slide protection screw
I) amp unit
J) Eyepieces
K) Eye cups
) Diopter adjustment
M) Filter slot (with dust cover)
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Preparing the Oxion microscope for use
Handling
Your microscope is a delicate product, please handle it with care.
Carefully remove the items from their packing and place them on a flat, firm surface. Please do not
expose the microscope to direct sun light, high temperatures, damp, dust or acute shake. Please
make sure the worktable is flat and horizontal.
When moving the microscope, use both hands to hold
the microscope. Hold it at the back handle
and the front of the base (see Fig. 1, point 1).
Caution! Holding the microscope at the table, stage
focusing knob or head will damage the
microscope.
For safety reasons, make sure the power switch is
turned off (“0”) and remove the plug before
Replacing the led or fuse, and make sure the unit is
not too warm.
Figure 1
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Assembling Steps
Euromex Microscopes will always try to keep the number of assembly steps for their customers as
low as possible but in some cases there are some steps to be taken. The steps mentioned below
are often not necessary but described for your convenience nonetheless.
Inserting/replacing the light source
1. Align the oriented pin and power pin on the light source to oriented holder and power socket,
and then push light source into arm smoothly and plug it thoroughly. When replacing the bulb,
turn off the main power and wait until the ED and holder cool down. Replace whole ED light
source if ED bulb is defect.
Mounting the objectives
1. Rotate the coarse focusing knob to lower the stage to the lowest position.
2. Install the objectives into the objective nosepiece from the lowest magnification to the highest
in a clockwise direction from the rear side of the microscope. When using the microscope, start
using the low magnification objective (4X or 10X) to search for specimen and focus, and then
continue with high magnification objective to observe.
The microscope head
The standard Oxion series configuration is supplied with the head assembled. However, if your
order contains the fluorescence it should be mounted first. The dovetail on the bottom side of
these parts fits into the slot on the top side of the other parts.
Placing the eye pieces
1. Remove the cover of eyepiece tube.
2. Insert the eyepiece into the eyepiece tube
3. When adjusting diopter by adjustable eyepiece, lock eyepiece with hexagon lock-screw to avoid
the eyepiece group from rotating in eyepiece tube.
The eyeshades
Each eyepiece has its rubber eyeshade. This prevents damage to the lens, and prevents stray light.
The eyeshade can simply be slipped over the eyepiece.
Connecting the power cord
The Oxion series microscopes supported a wide range of operating voltages: 100 to 240V. Please
use a grounded power connection.
1. Make sure the power switch is at“0” (OFF) before connecting.
2. Insert the connector of power cord into the Oxion’s power socket, and make sure it
connects well.
3. Insert the other connector into the mains socket, and make sure it connects well.
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Don’t use bend or twist the power cord, it will get damaged. Using the special cord supplied by
Euromex. If it’s lost or damaged, choose one with the same specifications.
Operation:
Setting up the illumination
1. Connect the Oxion microscope to a mains power source and turn on the main power
switch on.
2. Adjusting the light adjustment knob until the illumination is comfortable for observation.
Rotate the light adjustment knob clockwise to raise brightness. Rotate the light adjustment
knob counterclockwise to lower brightness
Caution: The maximum light intensity when using the 4x and 10x can damage the eyes! We
recommend using the neutral filter with these magnifications.
Place the specimen slide
1. Push the arm of the specimen holder backwards.
2. Release the arm slowly clamping the slide with the cover glass facing up.
3. Rotating the X and Y-axis knob will move the specimen to the center for alignment with the
center of the objective.
Focusing and slide protection
1. Select the objective 4x to the optical path.
2. Rotate the position screw to top, observe the right eyepiece with right eye. Rotate the
coarse focusing knob until the image appears.
3. Rotate the fine focusing knob for detailed focusing and lock the slide protection screw. The
slide protection screw protects the slide by limiting the travel of the table. This way the
objectives will not touch or break your slides.
Adjusting the focusing tension
The Oxion series microscope focusing knobs can be adjusted for tension. You can set it from light
to heavy according your own preference. Please note that when the specimen leaves the focus
plane after focusing or the stage declines itself, the tension should be set higher. To tighten the
focusing arm (more heavy), rotate the tension adjustment ring according to the arrowhead
pointed; to loosen it, please turn it in the reverse direction.
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The interpupillary distance
Using a binocular (or trinocular) tube is less tiring for the eyes than the use of a monocular tube. In
order to obtain a smooth “compound” image, one should go through the below steps.
The correct interpupillary distance is reached when one round image is seen in the field of view
(see image below). This distance can be set by either pulling the tubes towards each other or
pulling them from each other. This distance is different for each observer and thus should be set
individually. When more users are working with the microscope it is recommended to remember
your interpupillary distance for a quick set up during new microscopy sessions. The Oxion’s
swiveling eyepiece tube can be rotated 360º. You can select corresponding eye point height
according to your own preference.
Field of view before Field of view after
adjustment adjustment
The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To obtain the correct eye
point, move the eyes towards the eyepieces until a sharp image is reached at a full field of view.
People wearing glasses
When the eyeshades bother people wearing glasses, the Oxion series eyeshades simply can be
folded back to create more space.
Adjusting the diopter
Using a binocular (or trinocular) tube is less tiring for the eyes than the use of a monocular tube. In
order to obtain the right interpupillary setting, one should go through the below steps.
• Turn the diopter adjustment ring of the left eyepiece tube until the scale shows the same
reading as on the indicator.
• Close the right eye and focus the left tube by means of the coarse- and fine adjustment
knobs
• Close the left eye and focus the right tube with the diopter adjustment ring.
This procedure should be followed by each individual user. When more users are working with the
Oxion microscope it is recommended to remember your diopter setting for a quick set up during
new microscopy sessions.
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