Euromex bScope Fluo Manuel utilisateur
1
1. Installation of the fluorescence illuminator and microscope head
Most parts are already mounted on the microscope. Only two parts are packed separately. The
microscope head (fig. 1 number 1) and the fluorescence illuminator (fig. 1 number 2).
Fig. 1
1.1 Installation steps
2. Take the microscope with fluorescence attachment, fluorescence illuminator and head from
its packaging.
3. Place the head on the reflected illumination and fasten the screw (on the right side of the
microscope) with a hexagon wrench.
4. Install the eyepieces into the eyepiece tubes if this is not already installed (fig. 1 number 3).
5. Mount the fluorescence illuminator to the back of the fluorescence attachment and fasten
the screw with a hexagon wrench.
6. Plug the power cord into the power supply, but don’t turn it on yet.
2
2. Operation of reflected illumination system (LED fluorescence unit)
To get the best result when observing with fluorescence please pay attention to the following:
1. Select the fluorescence filter that matches the fluorochrome and fluorescent characteristic.
2. No autofluorescence specimen or device should be in the optical system, such as the cedar
immersion liquid wich has cyan autofluorescence.
3. Always turn off the transmitted illumination before turning on the reflected illumination
system.
2.1Set and adjust the fluorescence unit
The most important part of the unit is the fluorescence filters assembly. There are four
fluorescence filters, Blue, Green, Violet and Ultraviolet, mounted in the turret (fig. 2) and are
indicated as B, G, V and UV on turret. There is also a bright field option and it is indicated as 0 on
the turret.
The fluorescence filters in the turret corresponds with the LED in the fluorescence illuminator (see
table 1). There is a turret (fig. 2 number 1) on top of the fluorescence illuminator and has the same
indication of B, G, V and UV as of the turret for the fluorescence filters.
Fluorescence filters
LED light source
B
B
G
G
V
V
UV
UV
When selecting the filter, turn the turret (fig. 2 number 2) to the correct filter and choose the
corresponding LED (fig. 2 number 1) by turning the turret.
Fig. 2
Remark: For bright field observation please choose position 0 on the turret (fig. 2 number 2) and
switch off the fluorescence illuminator and turn on the transmitted illumination.
3
2.2Operation of the field diaphragm
The field diaphragm has been pre-centered and is ready to use, but due to possible vibration or
inclination during transport it may deviate, therefore always double check.
If it is not pre-centered, then please follow the following steps:
1. Use objective 10x.
2. Push in the field diaphragm switch (the one in the front, fig. 3 number 1), a light spot will be
visible in the field of view .
3. Adjust the centering if the light spot deviates from the center of the view of view (fig. 3
image a) by using the adjustment screws behind the filed diaphragm switch (fig. 3 number
2). The light spot should be centered as shown in fig. 3 image b.
4. Pull out the switch so that the image fills the field of view as shown in fig. 3 image c.
Fig. 3
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