AmScope B100 Manuel technique

B100/B200

Series Biological Microscope

Operation Instruction

To ensure the safety and obtain satisfactory performance, please study

this operation instruction thoroughly before your operation.

Contents

1.Purpose……………………………………………………………………………2

2.Specifications………………………………………………………………………2

3.Installation…………………………………………………………………………4

4.Operation……………………………………………………………………………4

5.Maintenance…………………………………………………………………………8

6.Troubleshooting……………………………………………………………………10

7.Outfits……………………………………………………………………………13

Seidentopf Binocular Head

QuadplexNosepiece

Objective

Clamp

Condenser Up- downKnob

Coaxial CoarseAnd

ElectricBase

Eyepiece

MainBody

Stage

StageMovingKonb

Condenser

Handleof IrisAperture Power SwitchAnd

Light Collector

Fig.1 B200Binocular Biological Microscope

FineFocusingKnob Light Controller

Condenser Up- downKnob

Coaxial CoarseAnd

ElectricBase

Eyepiece

Nosepiece

Objective

FineFocusingKnob

Monocular Head

MainBody

Clamp

Stage

Condenser

Light Controller

Power SwitchAnd

Light Controller

Fig.2 B100Monocular Biological Microscope

1. Purpose

B series biological microscope is designed for teaching, clinical verify. The series

microscope with original style, steady structure, convenient operation and clear

image is suitable for observing various biological specimens, and mostly applied in

school, college, hospital and family.

2. Specifications

2.1 Total magnifications

Objective

Total Magnifications

Eyepiece

10X

40X

100X

10X

40X

100X

400X

1000X

16X

64X

160X

640X

1600X

2.2 Objectives

Objective

Numerical

Aperture

(N.A.)

Objective Field

Resolving

Power

Working Distance

Field

Number

ф18

Field

Number

ф20

Achromatic

Objective

Plan

Objective

0.10

4.5mm

5mm

2.8µm

13mm

16mm

10X

0.25

1.8mm

2mm

1.1µm

6.3mm

2mm

40X (S)

0.65

0.45mm

0.5mm

0.42µm

0.44mm

0.66mm

100X

(Oil) (S)

1.25

0.18mm

0.2mm

0.22µm

0.3mm

0.42mm

2.3 The other specification

2.3.1 Mechanical tube length: 160mm

2.3.2 Conjugate distance: 195mm

2.3.3 Head: Monocular, Inclined 30°, Rotable 360°, Eyepiece can be fixed,

Anti-fungal systems.

Seidentopf binocular head 30°, Rotable 360°,

Interpupillary adjustable distance is 55-75mm, Diopter adjustable range

±5, Anti-fungal systems.

2.3.4 Nosepiece: Quadplex nosepiece

2.3.5 Mechanical stage: Size 142mm×134mm

X-Y travel 50mm×70mm

2.3.6 Focusing systems: Coaxial coarse and fine focusing knobs, Coarse stroke

22mm,

Fine division 2µm, Condenser up-down range 25mm

2.3.7 Condenser: Abbe condenser, N.A. 1.25, Adjustable aperture,

Aperture center is adjustable.

2.3.8 Mirror: Mirror diameterф52mm (optional)

2.3.9 Filter: Built in blue filer, ф38.5mm

2.3.10 Anti-fungal systems: Eyepiece and objective is anti-fungal, anti-fungal system

built in head.

2.3.11 Electric components: Input voltageAC85-265V, 50/60Hz

Output voltage DC1.2-6V

6V/20W halogen lamp

Rotation potentiometer with power switch

Fuse 2A ф5×20

3. Installation

B series microscope is packed integrate, and it is convenient for installation.

Open the package carton, and insert eyepiece into the eyepiece tube of the head

(eyepiece for monocular microscope has been inserted before packing). If objectives

don’t be fixed, please take objectives out from objectives packing box, and drive

them into the holes of nosepiece orderly and tightly according to the times.

Take out the oil bottle and dust cover, and place it properly for convenient use.

4. Operation

4.1 Electric components

Make sure the supply voltage meets the instrument’s requirement, then plug in it.

Rotation potentiometer with power switch used by the instrument is for protecting

lamp.

Mirror

Bracket

Mirror Base

Fig.4 Mirror

ClampHandleSpecimen Clamp Condenser

Vertical

MovingKnob

Horizontal

MovingKnob

Handleof

IrisAperture

Condenser

Up- downKnob

Fig.3 StageAndCondenser

4.2 Placing specimen (Shown in Fig.3)

Please place specimen on the stage, and the face with cover glass should be up, then

clip it with clamp. Clipping specimen should be careful in order to avoid spoiling

glass. Place specimen on the stage levelly, and make it into optical path by adjusting

vertical moving knob and horizontal moving knob.

4.3 Condenser (Shown in Fig.3)

4.3.1 Condenser up-down

Turn the condenser up-down knob to adjust the distance between condenser front and

the specimen, and change the equal illumination to obtain the best brightness.

4.3.2 Iris aperture adjustment

Stir the handle of iris aperture of condenser to

adjust size of iris aperture for adjusting the

contrast of the specimen.

4.4 Interpupillary distance and diopter

adjustment (Shown in Fig.5)

4.4.1 Interpupillar distance is different for everyone, so interpupillary distance should

be adjusted before using binocular microscope. Please take the two eyepiece tubes to

turn until the bright rings observed by two eyes are in superposition entirely.

▲B100 monocular microscope don’t need to be adjusted.

4.4.2 Diopter adjustment

As focusing for binocular, user should observe right eyepiece with right eye, and

make the right eyepiece clear by focusing adjustment, then observe the left eyepiece,

at the same time, adjust the diopter ring of the left eyepiece tube to make the image of

Diopter Ring

Eyepiece

Interpupillary

DistanceScale

Fig.5 InterpupillaryDistance

Adjustment And Diopter Ring

EyepieceTube

left eyepiece clear as same as the right eyepiece.

4.5 Focusing adjustment

Usually 10X objective should be used for focusing adjustment first. Take 10X

objective into bright path, then observe the specimen with right eyepiece by right eye

and turn coarse focusing knob to find the image, then turn slowly fine focusing knob

to make the image clear.

All objectives of our microscope are precisely adjusted, and approximate clear image

should be found with the other objectives after getting the clear image with 10X

objective. If the image isn’t enough clear with the other objectives, please turn fine

focusing knob slightly to find the satisfactory image.

▲Don’t turn left and right coarse and fine focusing knobs with different direction with

power at the same time, if so, the focusing system will be damaged.

▲Don’t directly pull objective to turn nosepiece when user changes the different

objectives, if so, optical quality of

microscope possibly be affected. The

right way is to take the tooth-like part

of the nosepiece to turn it, and make

the objective into correct position and

into the bright path.

4.6 Usage of the tension adjustment

ring (Shown in Fig.6)

The tension adjustment ring can adjust the tension of the coarse and fine focusing

unit to prevent the stage from sliding down automatically and to improve the comfort

Fig.6 Coarse A F Fnd ine ocusing

Left Coarse

Left Fine

Right Fine

Right Coarse

Tension

FocusingKnob

Adjustment Ring

FocusingKnob

KnobsAnd Tension Adjustment Ring

of operation. Rotation clockwise makes tension decrease, and by contraries, rotation

counterclockwise makes tension increase.

4.7 Usage of immersion oil objective

The 100X objective of B series microscope can be used for observation even if

without immersion oil. However, adding moderate immersion oil between the front

lens of 100X objective and the cover glass of specimen can make the image clearer.

Please pay attention that air bubble and impurity can’t be in the immersion oil,

otherwise, the image would be affected.

First of all, take 40X objective which has been focused well out from bright path,

then take 100X objective into bright path.At this time, nosepiece or stage moving

knob should be turned slightly, and also slightly turn fine focusing knob to clear away

the air bubble of immersion oil, otherwise, the air bubble would affect the image

badly.

▲After immersion oil used, the oil of specimen and the microscope surface should be

soon cleaned by absorbent cotton, lens paper, gauze or soft cotton cloth with

moderate mixture of pure industrial alcohol and ether (proportion 1:4)

▲Standard thickness 0.17mm cover glass should be chosen when high times objective

used, and thickness error should be within 0.01mm, otherwise, image definition

would be affected.

4.8 Mirror (Shown in Fig.4)

Mirror of B series microscope is optional, and it is used when electrical power isn’t

supplied.

Mirror doesn’t be suggested as illumination source when 100X objective used.

Take light collector down from base, then take mirror on base. Turn mirror to make

reflecting face to the light source, and make reflecting face and horizontal face as 45°

approximately to make the light to specimen as possible.The mirror has two sides, one is

a plane mirror, and the other one is a concave one. Concave mirror is suitable for

large-area outside light source, such as bright sky.

4.9 Points of attention after using

4.9.1 Power of microscope should be turned off after using, and the plug should be

pulled down. If immersion oil used, please clean objective and specimen soon.

Finally, cover microscope with dust cover.

4.9.2 Please take eyepiece and objectives out from microscope if user will stop using

it for a long time, and place eyepiece and objectives into drier with drying agent.

Cover microscope with dust cover.

5.Maintenance

5.1 Clean microscope

5.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft brush or

absorbent cotton or cleaned by absorbent cotton, lens paper with the mixture of

alcohol and ether (proportion 1:4).

5.1.2 Alcohol and ether all are burnt easily, please take them away from fire. Be

careful for turn on and off power.

5.1.3 Don’t clean painted metal and galvanizing metal with organic solvent such as

alcohol, ether or the mixture of the both. Silicon cloth or soft cleaning preparation is

suggested to clean it.

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