Agilent Technologies Bravo Platform Manuel utilisateur

Bravo Platform

User Guide

Original Instructions

Notices

Manual Part Number

G5562-90000 Revision D

January 2019

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or

translation into a foreign language) without

prior agreement and written consent from

Agilent Technologies, Inc. as governed by

United States and international copyright

laws.

Contact Information

Agilent Technologies Inc.

Automation Solutions

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

USA

Web:

https://www.agilent.com

Contact page:

https://www.agilent.com/en/contact-

us/page

Documentation feedback:

[email protected]

Acknowledgements

Microsoft® and Windows® are either

registered trademarks or trademarks of the

Microsoft Corporation in the United States

and other countries.

Warranty

The material contained in this document is

provided “as is,” and is subject to being

changed, without notice, in future editions.

Further, to the maximum extent permitted

by applicable law, Agilent disclaims all war-

ranties, either express or implied, with

regard to this manual and any information

contained herein, including but not limited

to the implied warranties of merchantability

and fitness for a particular purpose. Agilent

shall not be liable for errors or for incidental

or consequential damages in connection

with the furnishing, use, or performance of

this document or of any information con-

tained herein. Should Agilent and the user

have a separate written agreement with

warranty terms covering the material in this

document that conflict with these terms,

the warranty terms in the separate agree-

ment shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or

subcontract, Software is delivered and

licensed as “Commercial computer

software” as defined in DFAR 252.227-7014

(June 1995), or as a “commercial item” as

defined in FAR 2.101(a) or as “Restricted

computer software” as defined in FAR

52.227-19 (June 1987) or any equivalent

agency regulation or contract clause. Use,

duplication or disclosure of Software is

subject to Agilent Technologies’ standard

commercial license terms, and non-DOD

Departments and Agencies of the U.S.

Government will receive no greater than

Restricted Rights as defined in FAR 52.227-

19(c)(1-2) (June 1987). U.S. Government

users will receive no greater than Limited

Rights as defined in FAR 52.227-14

(June1987) or DFAR 252.227-7015 (b)(2)

(November 1995), as applicable in any

technical data.

Safety Notices

A WARNING notice denotes a haz-

ard. It calls attention to an operating

procedure, practice, or the like that, if

not correctly performed or adhered

to, could result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indicated

conditions are fully understood and

met.

A CAUTION notice denotes a hazard.

It calls attention to an operating proce-

dure, practice, or the like that, if not

correctly performed or adhered to,

could result in damage to the product

or loss of important data. Do not pro-

ceed beyond a CAUTION notice until

the indicated conditions are fully

understood and met.

WARNING

CAUTION

iiiBravo Platform User Guide

Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

About this guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

Accessing product user information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

About the Bravo Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Hardware overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Connection panel description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Liquid-handling heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Software overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2. Setting up the Bravo Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Workflow for setting up the Bravo Platform. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Starting up and shutting down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Creating or adding a Bravo device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Opening Bravo Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Creating and managing profiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Initializing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Setting teachpoints. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Verifying the gripper setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Configuring external robot access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

3. Preparing for a protocol run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Workflow for preparing a protocol run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Emergency stops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Planning for the protocol run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

About performing mock runs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

4. Maintenance and troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Routine maintenance and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Cleaning up after a run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Changing the Bravo head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Testing the safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

Replacing the fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

Retracting tip box stripper pins. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

Manually moving the head or gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Recovering from a head collision. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Troubleshooting hardware problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Troubleshooting hardware-related error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Reporting problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

Contents

iviv Bravo Platform User Guide

5. Using diagnostics software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Homing the liquid-handling head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Jogging the liquid-handling head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

Using the Move and Approach commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

Using the gripper controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

Performing a task using Bravo Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

A. Accessories and platepads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

Bravo accessories overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

Installing and setting up an Orbital Shaking Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

Using the teach plate to set the teachpoint for an accessory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

Installing and setting up a Vacuum Filtration Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

Setting up a Trash or Filter Plate Holder accessory. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134

Configuring a platepad for delidding. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139

Installing and configuring a platepad or Alignment Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140

Installing and configuring a Magnetic Bead Accessory. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143

Installing and setting up a Nested Rack Insert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147

Using the Manual Fill Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150

Setting up the Thermal Station (cooling pad) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151

Installing a Heating Shaking Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156

Setting up thermal and shaking stations (Inheco controller) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159

Installing and configuring the Peltier Thermal Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166

(Bravo SRT only) Setting up an LT tip box location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172

B. Autofilling accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175

Autofilling station overview and setup workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176

Configuring an autofilling station for the Bravo Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Setting up a Weigh Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182

C. Setting up the Barcode Reader. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185

About setting up the Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186

Installing or removing the Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188

Creating a profile for the Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189

Specifying the Barcode Reader location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191

Testing and optimizing barcode scanning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 192

D. AssayMAP Bravo Platform. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 197

AssayMAP Bravo Platform overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 198

Bravo 96AM Head overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200

AssayMAP cartridge overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205

Installing and using the Bravo 96AM Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 208

Verifying the setup in the Protein Sample Prep Workbench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215

Opening the Workbench and accessing the utilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 216

Requirements for partial-head liquid handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219

Contents

vBravo Platform User Guide

E. Diagnostics quick reference. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223

Bravo Diagnostics dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 224

Configuration tab quick reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225

Accessory Diagnostics dialog box. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 228

External Robots tab quick reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 229

IO tab quick reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230

Jog/Teach tab quick reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 232

Gripper tab quick reference. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 236

Profiles tab quick reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239

Processes tab quick reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 243

Aspirate task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247

Dispense task parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 250

Dispense to Waste task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 252

Mix task parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255

Pump Reagent parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258

Shake task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259

Vacuum Filtration Station task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 261

Wash Tips task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 264

Pin Tool task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268

AM Aspirate task parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271

AM Dispense task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 276

AM Mix task parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281

vii

Preface

This guide describes how to use the Bravo Automated Liquid Handling Platform,

also known as the Bravo Platform.

This preface contains the following topics:

•“About this guide” on page viii

•“Accessing product user information” on page x

About this guide

viii Bravo Platform User Guide

About this guide

Who should read this guide

This user guide is for people with the following job roles:

What this guide covers

This guide covers the description, setup, operation, and maintenance of the Bravo

Platform, including the following models:

•G5562A, G5563A Bravo Platform (RoHS-compliant)

•G5523A/G Bravo Platform

This guide does not provide instructions for unpacking and installation. For more

information about these topics, see the relevant user guides for these products.

What is new in this revision

and

Job role Responsibilities

Installer Unpacks, installs, and tests the device before it is used.

Integrator Configures hardware and writes software.

Lab manager,

administrator, or

technician

•Manages the automation system that contains the device

•Develops the applications that are run on the system

•Develops training materials and standard operating

procedures for operators

Operator Performs the daily production work on the device and solves

routine problems.

Feature and description See…

Revised the procedure for installing a

Thermal Station to include descriptions of

a new bracket.

“Setting up the Thermal Station

(cooling pad)” on page 151

Removed the installation and setup

sections from the AssayMAP Bravo

Platform section. For installation and

setup instructions, see the AssayMAP

Bravo Platform Installation Guide.

“AssayMAP Bravo Platform” on

page 197

About this guide

Bravo Platform User Guide

Software version

This guide documents Bravo Diagnostics version 19.1, which is included in the VWorks

Automation Control software v13.1 installer.

The G5562A, G5563A Bravo Platform requires the following software versions at a

minimum:

•Microsoft Windows 10 64-bit operating system

•VWorks Automation Control software v13.1

The earlier model G5523A/G Bravo Platform is validated on the Microsoft Windows 7

and Windows 10 64-bit operating systems.

Related guides

Use this guide in conjunction with the following:

•G5562A, G5563A Bravo Platform Safety and Installation Guide. Describes potential

safety hazards and how to avoid them, how to install the device, and how to install

the light curtain and shields.

•Automation Solutions Products General Safety Guide. Provides general safety

information and describes potential safety hazards that you might encounter when

using Agilent Automation Solutions products. A copy of this safety guide is

included with your shipment.

•VWorks Automation Control Setup Guide. Explains how to define labware and

labware classes, liquid classes, and pipetting techniques, and how to track and

manage labware in storage.

•VWorks Automation Control User Guide. Explains how to create protocols, and set

task parameters for each device in the system.

If you are using the Bravo 96AM Head, see “AssayMAP Bravo Platform” on page 197.

If the Bravo Platform is a device in a third-party system, see the relevant third-party

system guides.

Related information

For more information about... See...

How to access different formats

of this user guide

“Accessing product user information” on page x

Reporting problems “Reporting problems” on page 87

Bravo Platform safety

information

G5562A, G5563A Bravo Platform Safety and

Installation Guide

Accessing product user information

xBravo Platform User Guide

Accessing product user information

About this topic

This topic describes the different formats of user information and explains how to

access it for the Agilent Automation Solutions products.

Where to find user information

The Automation Solutions user information is available in the following locations:

•Knowledge base. The help system that contains information about all the

Automation Solutions products is available from the Help menu within the VWorks

software.

•PDF files. The PDF files of the user guides are installed with the VWorks software

and are on the software CD that is supplied with the product. A PDF viewer is

required to open a user guide in PDF format. You can download a free PDF viewer

from the internet. For information about using PDF documents, see the user

documentation for the PDF viewer.

•Agilent website. You can search the online knowledge base or download the latest

version of any PDF file from the Agilent website at www.agilent.com/chem/askb.

Accessing safety information

Safety information for the Agilent Automation Solutions devices appears in the

Automation Solutions Products General Safety Guide and in the corresponding device

safety guide or user guide.

You can also search the knowledge base or the PDF files for safety information.

Using the knowledge base

Knowledge base topics are displayed using web browser software such as Microsoft

Internet Explorer and Mozilla Firefox.

Note: If you want to use Internet Explorer to display the topics, you might have to allow

local files to run active content (scripts and ActiveX controls). To do this, in Internet

Explorer, open the Internet Options dialog box. Click the Advanced tab, locate the

Security section, and select Allow active content to run in files on my computer.

To open the knowledge base, do one of the following:

•From within VWorks software, select Help > Knowledge Base or press F1.

•From the Windows desktop, do one of the following:

–Windows 10. Select Start > All Apps > Agilent Technologies > VWorks Knowledge

Base.

–Windows 7. Select Start > All Programs > Agilent Technologies > VVWorks >

User Guides > VWorks Knowledge Base.

Accessing product user information

Bravo Platform User Guide

Opening the help topic for an area in the VWorks window

To access the context-sensitive help feature:

1In the main window of the VWorks software, click the help button . The pointer

changes to . Notice that the different icons or areas are highlighted as you

move the pointer over them.

2Click an icon or area of interest. The relevant topic or document opens.

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