VWR M4 Manuel utilisateur

VWR® M4 Spectrophotometer

Instruction Manual

European Catalogue Number

EU/UK/CH Plug: 634-1087

Version: 1.0.0

Issued: 24, June 2019

Legal Address of Manufacturer

Europe

VWR International bvba

Researchpark Haasrode 2020

Geldenaaksebaan 464

B-3001 Leuven

+ 32 16 385011

http://be.vwr.com

Made in China

 
                                     
Table of Contents 
Warning 1
Avoiding Electrical Shock 1
Package Contents 2
Unpacking 2
Installation 2
Intended use 2
Symbols and conventions 2
Product Specifications 2
Overview 3
Description 3
Getting Started 4
Turn On and Self-check 4
Important Guidelines 4
General Operating Instructions 4
Touch Screen Using Tips 4
Select Application 4
Basic Operation 5
Measurement Results Operation 6
Files Operation 7
Calibration and System Settings 8
Performance Verification 11 
Operation 13 
Photometry 13 
Quantitation 15 
Spectrum 19 
Troubleshooting 21 
Repair and maintenance 22 
Daily Maintain 22 
Spare Parts Replacement 22 
User replaceable accessories and spare parts 23 
Technical service 23 
Warranty 24 
Compliance with local laws and regulations 24 
Equipment disposal 25 
Functions 26 
Installation 26 
Install and Uninstall VWR Software Multi Wave Pro 26 
Run VWR Software Multi Wave Pro 29 
Introduction 30 
Main Interface 30 
Menus Bar and Tools Bar 30 

 
                                     
General Operating Instructions 31 
Operation 33 
Quantitative Analysis 33 
Kinetics Analysis 35 
Spectrum Scan 36 
Multi-wavelength Analysis 37 
DNA/Protein Analysis 38 
Energy Scan 39 
Other Functions 40 
APPENDIX 1 40 
APPENDIX 2 41 
 

Warning

Avoiding Electrical Shock

Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

VWR recommends against the use of M4 Spectrophotometer.

To avoid electrical shock

Do not open the device.

Disconnect the device from the mains supply before carrying out maintenance work or

changing the fuses.

The inside of the device is a high-voltage area Danger!

Do not use the device if it is damaged, especially if the main power cable way is in any

damaged or defective.

Repairs may only be carried out by the service technicians from your local VWR office and

authorized contractual partners.

The device must be connected to a power outlet that has a protective ground connection.

If the equipment is used in a manner not specified by the manufacturer, the protection provided

by the equipment may be impaired.

Avoiding Damage to the Instrument

Do not allow any liquid to enter into the device.

Do not operate the device in a hazardous location or potentially explosive environment.

Package Contents

Description

Quantity

Catalogue-No

Spectrophotometer

1PC

634-1087

Glass Cuvette

4PCS

634-6013

Quartz Cuvette

2PCS

634-6018

Power Cord ( Euro Plug )

1PC

on request

Power Cord ( UK Plug )

1PC

on request

Power Cord ( CH Plug )

1PC

on request

Software CD-ROM

1PC

N/A

USB Cable

1PC

N/A

Instruction Manual

1PC

N/A

Dust Cover

1PC

on request

Unpacking

Open the package, according to carefully check the packaging packing list items, if found inside the packaging are

missing or damaged items please contact your local VWR office and authorized contractual partners.

Installation

Placement

Place the instrument on the stable table carefully.

Install printer (Optional)

Check to confirm instrument power switch is turned off, connect the printer’s data cable to the Instrument’s

serial/USB port.

Connect the power cord

Check to confirm instrument power switch is turned off, the power cord plug into two separate power interface and

power supply socket apparatus.

Intended use

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION This symbol indicates a potential risk and alerts you to proceed with caution^

CAUTION This symbol indicates the presence of high voltage and warns the user to proceed

with caution

CAUTION This symbol indicates risks associated with hot surfaces

Product Specifications

Optical System

Spilt Beam

Wavelength Range

190-1100nm

Band Width

2nm

Stray Light

≤0.08%T @ 220nm & 360nm

Photometric Range

0 to 200%T, -0.3 to 3.0A, 0 to 9999C

Wavelength Accuracy

±0.5nm

Photometric Accuracy

±0.4%T or ±0.004A @ 1A

Stability

0.002A/h @ 500nm

Memory

236KB

Language

English, French, German, Spanish, Portuguese, Chinese

Display

TFT Colour LCD (5”, Touch Screen)

Interface

USB-A, USB-B,RS-232

Measuring Procedure

Photometry, Quantitation, Spectrum Scan

Power Supply

AC 100~240 V, 50~60 Hz

Dimension

456×360×185

Weight

10.5kg

Work Environment

15 to 35℃, 15 to 70% relative humidity

Store Environment

-10 to 50℃, 15 to 70% relative humidity

This instrument is compliant to the European Directives on Low Voltage Directive 2014/35/EU,

Electromagnetic compatibility 2014/30/EU.

Restriction on use of Hazardous Substances RoHS 2011/65/EU and their amendments.

Overview

M4 Spectrophotometer used in Chemistry, Pharmaceuticals, Biochemical, Metallurgy, Light Industry, Textile,

Material, Environments, Medical, Education and some other fields for Quality Control laboratories.

Description

Front View

Touch

screen

Lid of the

Compartment

Pull Rod

Lid of Lamp

Room

Right View

USB Port

(for USB Disk/

USB Printer)

Power

Switcher

Rear View

Fuse Seat

Power

Socket

Print Port

USB Port

(for PC)

Getting Started

Turn On and Self-check

Switch on the power. Self-check includes the following steps: Turn On Lamp → Locating Filter Disc → Locating

Automatic Sample Holder (If Installed) → Get Dark Current → Locating Wavelength → Check Energy → Check

System baseline.

Important Guidelines

Reagents and dilution buffers can cause cauterization and other damage to health.

Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious damage to

health.

During sample preparation, measuring procedures and maintenance and cleaning work, observe all local

laboratory safety precautions (e.g. wear protective clothing and gloves, use of disinfectant) regarding the

handling of sample material.

Dispose of measuring solutions and cleaning and disinfectant materials in accordance with the relevant

local laboratory regulations.

General Operating Instructions

Touch Screen Using Tips

The entire screen can be started with a touch. To make a choice, use your nails, fingertips, pencil, or stylus to

press the screen. Don't press the screen with sharp objects (such as ball point).

Select Application

Main Interface, press the icon to select application.

Photometry Measure the absorbance or transmittance of the sample.

Quantitation Establish the standard curve and measure the concentration of the sample.

Spectrum Scan the sample in a wavelength range.

File Manage files stored in the instrument or USB disk.

System System calibration and setup.

Performance verification Verify the performance of the instrument.

Basic Operation

Home Back to main interface.

Return Back to the previous interface.

Page Up/Down Go to previous/next page.

Measurement Results Operation

Open Open result(s) from internal/USB memory.

Save Save result(s) to internal/USB memory.

Print Print result(s).

Delete Delete selected result(s).

Rename, Print and Delete Results

Rename a Sample:

List interface, press the area Name, key in the sample name (Up to

8 characters).

Print the Measurement

Report:

List interface, press the icon .

Delete sample(s):

List interface, press the Check Box, and press the icon .

Open Results

Open:

1. List interface, press the icon .

2. Press the icon internal memory/USB memory to select the

memory which the file saved.

3. Press file lists to select, press the button Open.

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