VWR HIGH PERFORMANCE PIPETTOR Manuel utilisateur
VWR®HIGH PERFORMANCE PIPETTOR
USER GUIDE
Light-Weight Ergonomics
Precise & Reliable
Secure Results
EN
2
VWR®HIGH PERFORMANCE PIPETTOR
Contents Page
1- INTRODUCTION 3
2- PARTS CHECK LIST 3
3- DESCRIPTION 4
4- SETTING THE VOLUME 6
5- USER ADJUSTMENT 8
6- PIPETTING 10
7- GENERAL GUIDELINES FOR GOOD PIPETTING 12
8- ACCESSORIES AND STAND 13
9- GLP FEATURES 13
10- TROUBLESHOOTING 14
11- LEAK TEST 15
12- MAINTENANCE 15
13- CLEANING AND STERILIZATION 21
14- SPECIFICATIONS 25
15- SPARE PARTS 26
16- MAINTENANCE & CALIBRATION 27
17- TECHNICAL SERVICES 27
18- WARRANTY 27
19- COMPLIANCE WITH LOCAL LAWS AND 28
REGULATIONS
20- EQUIPMENT DISPOSAL 28
3
USER’S GUIDE
The VWR High Performance Pipettor is an air displacement mechanism and is used
with disposable pipette tips.
This pipettor line provides:
• Light and comfortable body
• Low pipetting forces ensuring ergonomics and users’
well-being
• Unique patented Volume locking mecanism for results you can trust
Eight single channel models cover a volume range from 0.2 µL to 10 mL.
Eight multichannel models (4 x 8-ch and 4 x 12-ch) cover a volume range from
0.5 µL to 300 µL.
Just take a moment to verify that the following items are present:
Single models
• VWR High Performance Pipettor,
• User’s Guide,
• Certificate of conformity,
• Calibration Key,
• Tip-ejector extention for 2µL and 10µL models.
Multichannel models
• VWR High Performance Pipettor,
• User’s Guide,
• Ejector spacer for short collar tips (only for multichannels models 10 µL),
• Certificate of conformity,
• Calibration key.
1 - INTRODUCTION
2 - PARTS CHECK LIST
4
VWR®HIGH PERFORMANCE PIPETTOR
3 - DESCRIPTION
For 2µL and 10µL Single channel models, a tip-ejector
extention is supplied to fit with short tips models.
To fit a tip-ejector extention:
Slide the extention over the tip-holder
Push the extention firmly onto the end of the tip-ejector
until it clicks into place (see figure 2)
FIGURE 2
Single channel models Multichannel models
Color-coded
push-button
Lock/Unlock
mechanism & Tip-
ejector button
Handle
Connecting nut
Tip-holder
Ejector clip
Ejector support
Ejector lock
Cover
Tip-ejector
Tip-holders
FIGURE 1
Counter
3 - DESCRIPTION
5
USER’S GUIDE
To remove a tip-ejector extention:
Gently twist the extention
Pull it away from the pipettor
Ejector spacer for Multichannel models (X10µL only)
Multichannel models fit with long collar tips. If you use short collar tips, you might
have to insert the ejector spacer especially indicated for that:
• Remove the tip-ejector, keep both ejector locks depressed; pull the tip-ejector
down.
• Insert the broad ejector spacer and click it to the tip-ejector.
•
To refit the tip-ejector, gently re-insert the tip-ejector
vertically into the rails of
the ejector support.
FIGURE 3 Long collar tips Short collar tips
Ejector spacer
6
VWR®HIGH PERFORMANCE PIPETTOR
The volume of liquid to be aspirated is set using the volumeter. The digits are colored
either black or red to indicate the position of the decimal point, depending on the
model (see examples below).
4 - SETTING THE VOLUME
MODEL Color of volumeter digits
Black Red Increment
2µl µL 0.01 µL 0.002 µL
10µl to 20µl µL 0.1 µL 0.02 µL
8X-10µl, 8X-20µl
12X-10µl, 12X-20µl µL 0.1 µL 0.02 µL
100µl, 200µl, 8X-200µl, 12X-200µl µL - 0.2 µL
8X-300µl, 12X-300µl µL - 0.2 µL
1000µl 0.01 mL mL 0.002 mL
5000µl 0.01 mL mL 0.002 mL
10 mL mL 0.1 mL 0.02 mL
0
7
5
X10µl
7.5 µL
1
2
5
X20µl
12.5 µL
X200µl
1
2
5
125 µL
X300µl
1
2
5
125 µL
Single models Multichannel models
1
5
2µl
1.25 µL
2
0
7
5
10µl
7.5 µL
1
2
5
20µl
12.5 µL
100µl
0
7
5
75 µL
200µl
1
2
5
125 µL
1000µl
0
7
5
0.75 mL
5000µl
1
2
5
1.25 mL
10mL
0
7
5
7.5 mL
7
USER’S GUIDE
Lock system:
For trusted results, the volume selection is lockable.
To obtain maximum accuracy when setting the volume, proceed as follows:
> when decreasing the volume setting, slowly reach the required setting, making
sure not to overshoot the mark.
> when increasing the volume setting, pass the required value by 1/3 of a turn and
then slowly decrease to reach the volume, making sure not to overshoot the mark.
> To adjust perfectly the last digit, it is even more precise to do so on the Lock
position.
First step, with the thumb, unlock the
tip-ejector button by pushing it up
Second step, the volume is set by turning
the push button
3 Third step, push down the tip-ejector
button.
The selected volume is locked.
Unlocked
Position
8
VWR®HIGH PERFORMANCE PIPETTOR
The calibration of the VWR High Performance Pipettor has been performed with
distilled water and very high precision volumetric instruments. Adjusting the pipettor
can be necessary for different solutions due to their density, viscosity, surface
tension and/or vapor pressure etc. Calibration is sometimes recommended when it
is used in high altitudes or with special tips. It can also be recalibrated when long
pipette tips are used. Performance testing should take place in a draught-free room
at 15-30°C, constant to ± 0.5°C and humidity above 50%.
To adjust the volume setting:
Remove the push button.
Use the calibration key to take off the block cover.
a. Insert the metal rod into the calibration tool on the hexagonal
side.
b. Engage the two rectangular hooks of part 2 into the two
holes of the block cover. You should feel them clipped on firmly
in the hidden part of the pipettor (see figure 5).
c. Make sure to hold the part 1 at the top cap, along the part 2.
d. Turn the part 2 counterclockwise slowly to remove the block
cover (see figure 6).
Put the block cover apart.
Part 1
Part 2
5 - USER ADJUSTMENT
FIGURE 6
FIGURE 5
Part 1
Part 2
9
USER’S GUIDE
Use the calibration key to adjust the pipettor
a. Insert the metal rod into the calibration key on the
circle tip side (see figure 7).
Lock it into place. You should feel the
internal part of the calibration key clamped and clipped
on firmly. If it is not the case, turn it counterclockwise
slowly. Then, turn the plastic connecting nut of the part
1 slowly until it’s locked in place (see figure 8).
b. Hold the connecting nut of the part 1 with
one hand
and turn the part 2 with the other one
according to the correction needed (see figure 9).
Once the desired volume is set, remove the
calibration key and put back the block cover
using the hexagonal side. Turn it clockwise to
lock the block cover. Put back the push button.
With reference water, one revolution
(1/8 turn of the calibration tool) of the
volume setting corresponds to:
FIGURE 9
FIGURE 8
FIGURE 7
Volume range Vol. per 1/8 turn
(equivalence in µL)
2µl 0.012
i.e: 1 full turn is 0.096
10µl 0.047
20µl 0.120
100µl 0.48
200µl 1.20
1000µl 4.75
5000µl 23.8
10ML 48
8X-10µl
12X-10µl 0.058
8X-20µl
12X-20µl 0.120
8X-200µl
12X-200µl 1.20
8X-300µl
12X-300µl 1.90
Part 1
Part 2
10
VWR®HIGH PERFORMANCE PIPETTOR
6 - PIPETTING
Fitting the tips
VWR High Performance Pipettor have been designed to fit VWR tips.
Pre-rinse the tips
Some liquids (e.g.protein-containing solutions and organic
solvents) can leave a film
of liquid on the inside wall of the tip; pre-rinse the tip to minimize any errors that
may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it to
waste. Tips will not fall off nor will they have to be manually positioned.
Make sure first that the pipettor is calibrated with the tips that you are using. Then,
subsequent volumes that you pipettor will have levels of accuracy and precision
within specifications. Using other tips may require a validation of the pipetting
system.
1st stop
2nd stop
1st stop
FIGURE 10
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