Thermo Scientific GeneTitan Scanner Guide de l'utilisateur
For Research Use Only. Not for use in diagnostic procedures.
GeneTitan™Scanner
USE AND MAINTENANCE GUIDE
Catalog Numbers 00-0442 and 00-0443
Publication Number MAN0018315
Revision A.0
Affymetrix Inc. | 3450 Central Expressway | Santa Clara, CA 95051 | USA
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL,
INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,
INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018315
Revision Date Description
A.0 31 December 2019 New publication.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept
the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Microsoft and Windows
are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries.
Smart
Shutter is a trademark of
Sutter Instrument Company. Cermax is registered trademark of Excelitas Technologies Corp.
©2019 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■CHAPTER 1 Product information ....................................... 5
Instrument overview ............................................................. 5
Parts of the instrument system ................................................... 5
GeneTitan™Scanner ........................................................ 6
Workstation setup .......................................................... 8
Lambda LS light source ..................................................... 10
Lambda SC shutter control .................................................. 10
Uninterruptible power supply ............................................... 12
■CHAPTER 2 GeneTitan™Scanner operation .......................... 13
Guidelines .................................................................... 13
Lambda system operation ....................................................... 13
GeneChip™Command Console™(GCC) ............................................ 14
Start the scanner .............................................................. 15
Scan an array plate ............................................................. 15
Shut down the scanner ......................................................... 16
■CHAPTER 3 Maintain the Instrument ................................. 17
GeneTitan™Maintenance ........................................................ 17
Monthly .................................................................. 17
Every six months .......................................................... 17
Lambda LS xenon arc lamp ...................................................... 18
Replace the Lambda LS fuse ................................................ 18
Xenon lamp replacement ................................................... 19
Lambda SC
Smart
Shutter™Controller ............................................ 24
Replace the Lambda SC fuse ................................................ 24
■APPENDIX A Troubleshooting ......................................... 25
GeneTitan™Instrument support files for troubleshooting ............................ 25
Log files .................................................................. 25
GeneChip™Command Console™log files ...................................... 25
Other GeneChip™Command Console™files ................................... 25
GeneTitan
™
Scanner Use and Maintenance Guide
3
GCC log files for the GeneTitan™Scanner ..................................... 26
GeneTitan™Scanner ....................................................... 26
■APPENDIX B Parts and materials ..................................... 28
Replacement parts ............................................................. 28
■APPENDIX C Instrument specifications .............................. 29
Environmental requirements .................................................... 29
Electrical requirements ......................................................... 30
Component clearances required for installation and maintenance .................... 31
Lambda LS xenon arc lamp specifications ......................................... 31
Lambda SC controller cables .................................................... 32
■APPENDIX D Safety ..................................................... 33
Symbols on this instrument ..................................................... 33
Standard safety symbols .................................................... 33
Additional safety symbols ................................................... 34
Location of safety labels .................................................... 35
Conformity symbols ........................................................ 36
Safety information for instruments not manufactured by Thermo Fisher Scientific ..... 36
Instrument safety .............................................................. 36
General .................................................................. 36
Physical injury ............................................................ 37
Electrical safety ........................................................... 38
Cleaning and decontamination ............................................... 39
Instrument component and accessory disposal ................................ 39
Laser safety ............................................................... 40
Safety and electromagnetic compatibility (EMC) standards .......................... 40
Safety standards ........................................................... 41
EMC standards ............................................................ 41
Environmental design standards ............................................. 41
Chemical safety ................................................................ 42
Biological hazard safety ......................................................... 43
■APPENDIX E Documentation and support ............................ 44
Related documentation ......................................................... 44
Customer and technical support ................................................. 44
When to call technical support ................................................... 45
Limited product warranty ....................................................... 45
Contents
4
GeneTitan
™
Scanner Use and Maintenance Guide
Product information
Instrument overview
The Applied Biosystems™ GeneTitan™ Scanner is a high-throughput imaging device
with multi-axis robotic handling capability for imaging 24-, 96-, mini 96, and 384HT-
array plate formats. The scanner is designed to measure the uorescence signals of
labeled DNA targets that are hybridized to the array plates. The illumination source
of the GeneTitan™ Scanner is an external, high-intensity xenon arc lamp. The
GeneTitan™ Scanner is also equipped with a lter slider that allows up to 2 optical
lters sets to detect uorescence intensity of samples in array plate layouts for
downstream analysis.
Parts of the instrument system
The GeneTitan™ Scanner System consists of:
• GeneTitan™ Scanner
• Computer workstation and monitor—Windows™ 10 IoT operating system with
GeneChip™ Command Console™ software (GCC) and Microsoft™ Oce 2016
preinstalled
• Lambda LS— Stand-alone xenon arc lamp and power supply that is equipped
with a stand-alone SmartShuer™ system
• Lambda SC—SmartShuer™ Controller
• Uninterruptible power supply (UPS)
1
GeneTitan
™
Scanner Use and Maintenance Guide
5
2
1
1Yellow LED light
2Green LED light
GeneChip™ Command Console™ software controls the power to the GeneTitan™
Scanner. Power the instrument on or o using the software.
Instrument status indicator
The two indicator lights in the lower left front of the instrument display various
paerns of light and color indicating dierent instrument conditions. The following
table denes the meaning for the status light paerns.
Light indicator Instrument status
Solid green Ready or idle state
Solid yellow Initializing/homing the instrument
Blinking green Imaging in progress
Blinking yellow Hardware or software error state
GeneTitan™
Scanner
Chapter 1 Product information
Parts of the instrument system
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GeneTitan
™
Scanner Use and Maintenance Guide
GeneTitan™Scanner drawer door
The GeneTitan™ Scanner drawer door is on the left side of the scanner. The drawer
door is controlled by the GCC software, and opens during operation to receive or
expel the array plate and scan tray combination.
1
2
1Scanner drawer door
2Scanner drawer extended
WARNING! Crushing Injury Hazard. Do not place hands or face near the load
tray when loading plates into or ejecting plates from the instrument.
Chapter 1 Product information
Parts of the instrument system
1
GeneTitan
™
Scanner Use and Maintenance Guide
7
Connections
8
1
2
3 4
7
65
Figure 1 GeneTitan™Scanner back connections
1Power receptacle—connect to the Lambda LS
2Power cable and circuit breaker button
3BNC port for CCD camera cable to control the external shutter—connect to the Lambda
SC TTL IN receptacle
4Kvaser USB port for motion controller communication cable—connect to the computer
5Power receptacle for the GeneTitan™Scanner—connect to the UPS
6USB port for the internal devices communication cable—connect to the computer
7FireWire port for CCD camera communication cable—connect to the computer
8Light guide—connects to the Lambda LS
CAUTION! FireWire (IEEE 1394 High-Speed Serial Bus) and USB cables are not
locking connectors. Ensure that the FireWire and USB cables are plugged
securely and in the correct orientation in both the scanner and the workstation.
Avoid snagging or pulling cable during scanner operation.
The instrument workstation consists of a separate computer, keyboard, mouse, and
monitor connected to the GeneTitan™ Scanner through its rear utility panel. The
workstation includes the following pre-installed software:
• Windows™ 10 Enterprise 2016 LTSB operating system
• Microsoft™ Oce 2016
• GeneChip™ Command Console™ software (scanner control)
Workstation setup
Chapter 1 Product information
Parts of the instrument system
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GeneTitan
™
Scanner Use and Maintenance Guide
Computer back panel connections
1
5
2
7
3
10
8
9
6
4
1Serial port—connect to Lambda LS
2USB 2.0 port (2)—connect to the keyboard and mouse
3USB 3.0 port—connect to scanner
4USB 3.0 port—spare
5Ethernet port—connect to local network
6USB 3.0 port—connect to Kvaser USB cable from scanner
7USB 2.0 port (1)—spare
8DVI and HDMI video ports—connect to monitor
9FireWire ports (2)—connect one to scanner
10 Power cable connector—connect to the UPS
Note: Three additional USB 2.0 ports, and 1 additional USB 3.0 port are in the front of the
computer.
Chapter 1 Product information
Parts of the instrument system
1
GeneTitan
™
Scanner Use and Maintenance Guide
9
The Lambda LS is a stand-alone xenon arc lamp and power supply system that is
equipped with a SmartShuer™ component.
The system consists of a xenon-arc lamp, lamp housing, cold mirror, SmartShuer™,
and power supply. The Lambda LS is designed for use with a liquid light guide that
transmits at, intense illumination to the optical train of the GeneTitan™ Scanner.
The Lambda LS xenon arc lamp illuminator system is installed by a qualied eld
service engineer and placed near the scanner. The lamp system must not be placed on
the oor. Do not move the lamp system after installation.
Lambda LS light source connections
4
3
1
2
1Light guide connection
2Lens tube
3Liquid light guide—connect to the back panel of the scanner (see the following caution
note)
4Serial connection—connect to the Lambda SC
CAUTION! The liquid light guide is a fragile ber optic cable. Do not kink or
bend the cable to less than 6 inches (15.25 cm) radius. Do not place objects on
the light guide.
The Lambda SC is a microprocessor-controlled shuer driver for the SmartShuer™
device in the Lambda LS. It oers enhanced performance that allows programmable
control over the exposure time, the delay between exposures, the number of
exposures, the mode of triggering, and the mode of shuer opening.
IMPORTANT! Always keep the Manual Shuer Control switch in the AUTO position.
Lambda LS light
source
Lambda SC
shutter control
Chapter 1 Product information
Parts of the instrument system
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GeneTitan
™
Scanner Use and Maintenance Guide
Table des matières
