Thermo Scientific GENESYS 30 Manuel utilisateur

GENESYS 30

Spectrophotometer

User Guide

269-317300 Revision A March 2015

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WARNING Avoid an explosion or fire hazard. This instrument or accessory is not

designed for use in an explosive atmosphere.

For Research Use Only. This instrument or accessory is not a medical device and is not intended to be used

for the prevention, diagnosis, treatment or cure of disease.

Thermo Scientific GENESYS 30 User Guide 1

GENESYS 30 User Guide

The Thermo Scientific™ GENESYS™ 30 visible spectrophotometer is designed to measure

transmittance and absorbance in the range 325 nm to 1100 nm. Typical samples are

contained in a square plastic or glass cuvette with a pathlength of 10 mm. Sample holders that

support measurement of solutions in test tubes or longer cuvettes, and limited options for

measuring transmitting solid samples, are also available.

The spectrophotometer incorporates control software capable of displaying absorbance or

transmittance data as single wavelength measurements or as a wavelength scan. It can also

perform simple mathematical operations on data obtained at one or multiple wavelengths and

present the results of these calculations. Additionally, the software supports quantitative

analyses using either a user-entered calibration factor or establishment of a calibration

equation using between one and six measured standard solutions.

Data from the spectrophotometer’s measurements are displayed on a high resolution color

screen (32 bit, 800 x 480 pixels) and may also be printed using an optional thermal printer

accessory or saved to a flash memory device through a USB port conveniently located on the

front of the instrument.

All aspects of the users’ interactions with the GENESYS 30 visible spectrophotometer have

been carefully considered and accommodated in the design of the instrument. Accessibility of

the sample compartment, layout and labeling of the keypad, and placement of the tools and

features in the software are all optimized to provide the best possible user experience for the

instrument user.

2GENESYS 30 User Guide Thermo Scientific

Table of Contents

•“Terminology” on page 3

•“About the Keypad” on page 4

•“Live Display” on page 8

•“Scan” on page 11

•“Quant” on page 13

•“Fixed” on page 17

•“OD600” on page 20

•“Library” on page 22

•“Settings” on page 23

•“SmartStart” on page 30

•“Optional Accessories” on page 31

•“Halogen Lamp” on page 35

Thermo Scientific GENESYS 30 User Guide 3

Terminology

Field An area on the screen is where you can enter a value.

Tool An icon on the screen where you can select something either to choose a

setting or to make something happen.

Navigate Press to move the focus between tools and fields on the

screen.

Focus The field or tool that is selected is described as being “in focus.” It is indicated

by an orange line around it.

Select To select a tool or field, navigate to it and press

Save Tool Some applications allow the user to save methods to an on-board method

library or to a USB memory device. Methods saved to a USB memory device

can be transfered to other GENESYS 30 instruments or archived on a PC.

The Scan, Fixed and Quant applications also support saving tables of data

directly to a USB memory device for off line storage or processing. When you

select the save tool, a dialog box will prompt you to choose whether to save

the method or the data and direct you through the necessary steps to name

methods before saving. Method files are saved with user-specified names.

Data files are automatically named as Scan_, Fixed_, or Quant_ followed by a

date and time stamp. Data files cannot be saved with a user-specified name.

Focus

Message bar - look here for

instructions and reports

Baseline status

indicator

Tools

4GENESYS 30 User Guide Thermo Scientific

About the Keypad

Important features of your software:

Press Autozero to record a blank value in Live Display, Fixed, OD600, Analyzer

and Quant modes. Record a baseline in Scan mode.

Press the Home key to return to the home page at any time. If going to the

home menu will cause you to lose result data, the software will warn you and

give you the opportunity to cancel the action before going to Home.

When the number in a field is highlighted you can simply start keying in

numbers and it will be over-written.

If there is a line cursor between or next to the value, you need to use to

erase digits one by one. Also, if you erase a value completely and then press

, the value that was in the cell before you erased it will be restored.

• In any text or number entry fields causes one character or number to

be erased per press.

• In alerts or dialogs where the user is not entering numeric or text data and

there is a Cancel button displayed on the screen, pressing on the

keypad has the same effect as navigating to Cancel and pressing .

Number pad: enter numeric

values

Nav keys: controls navigation

on the instrument screen

Autozero: measure a

blank value or

baseline scan

Home: go to the home screen

Stop: stop the

current operation

Start: start the measurement specified using

the current settings

Backspace: remove

displayed values for

new data entry

Print: print the method or results

to the on-board printer with a date

and time stamp

Enter: accept user-entered values

Thermo Scientific GENESYS 30 User Guide 5

About the Home page

In addition to starting measurements in data modes, the start button acts as

short-cut to load a method when in the Library.

Note: If you press the measure button when the blank/baseline is not valid the

software plays the unhappy tone through the speaker and flashes the blank

indicator three times.

Stop

Enter

Options are clearly labeled with the name of the measurement modes or functions

that they execute. Use the nav keys to select the desired application and press to

start it.

6GENESYS 30 User Guide Thermo Scientific

This feature is not yet available. See Fixed for detailed information.

See OD600 for detailed information Load or transfer saved methods in the

on-board memory or an inserted USB

memory device. See Library for detailed

information.

See Live Display for detailed information. See Quant for detailed information.

Thermo Scientific GENESYS 30 User Guide 7

Access the various functional settings for

instrument behavior including connecting to

a computer running control software. See

Settings for detailed information.

See Scan for detailed information.

You may choose to save selected methods to

a special SmartStart™ menu which will be

displayed instead of the home screen at

start-up. This home screen option brings you

to that same SmartStart menu.

8GENESYS 30 User Guide Thermo Scientific

Live Display

Live Display shows a continuously updating value for the transmittance, absorbance or

concentration of a sample. The instrument takes a new measurement every two (2) seconds.

The value on the screen flashes to indicate that a new reading is being displayed.

%Transmittance or Absorbance Measurements

1. Select a measurement wavelength.

2. Select the Mode (%T or ABS).

3. Place a cuvette with a blank in the measurement position, close the lid and press .

4. Remove the blank cuvette, place the sample cuvette in the measurement position and

close the lid.

Live measurements begin automatically.

The %T or ABS value is displayed on the screen.

5. To make measurements of additional samples, simply put the cuvette with the new

sample in the measurement position, close the lid and wait for the displayed value to flash

to indicate that the measurement has been made.

Measurement mode

selection and indicator tool

Message bar - look here for

instructions and reports

Measured

ABS/%T value

Measurement wavelength

Baseline status

indicator

Tip If you are using the test tube holder you may not need to close the lid when

making measurements.

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