Sutter Instrument Lambda DG-4Plus/USB Manuel utilisateur

One Digital Drive

Novato, CA 94949

Voice: 415-883-0128 Web: www.sutter.com

Fax: 415-883-0572 Email: info@sutter.com

Lambda DG-4Plus/USB

and

Lambda DG-5Plus/USB

Ultra-High-Speed Wavelength

Switching Xenon Arc Light Source

System

Operation Manual

Rev. 1.01b3 (20190607)

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)

LAMBDA-10®and SmartShutter®are registered trademarks of Sutter Instrument

Company.

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)

iii

DISCLAIMER

The ultra high speed wavelength switching xenon arc lamp Lambda DG-4PLUS/USB/DG-

5PLUS/USB system is designed for the specific use as a microscope illuminator and optical

wavelength switcher, and no other use is recommended.

This instrument should only be used in a laboratory environment for use on animal tissues.

It is not intended for, nor should it be used in, human experimentation or applied to humans

in any way. This is not a medical device.

Do not open or attempt to repair the instrument without expressed and explicit instructions

from Sutter Instrument Company. Extreme heat and high voltages are present and could

cause injury.

Do not allow unauthorized and or untrained operatives to use this device.

Any misuse will be the sole responsibility of the user/owner and Sutter Instruments assumes

no implied or inferred liability for direct or consequential damages from this instrument if it

is operated or used in any way other than for which it is designed.

SAFETY WARNINGS AND PRECAUTIONS

Electrical

Operate the Lambda DG-4PLUS/USB and DG-5PLUS/USB using 100- 240 V AC, 50/60

Hz line voltage. This instrument is designed for connection to a standard laboratory

power outlet (Overvoltage Category II), and because it is a microprocessor--controlled

device, it should be accorded the same system wiring precautions as any 'computer type'

system. A surge protector and power regulator are recommended.

Fuse Replacement: Replace only with the same type and rating:

5 x 20 mm glass tube, time delay (medium), time lag, or “slow blow”)

Rating:

Xenon

Lamp

Power

Rating

Line Voltage: 100 – 240 VAC

Fuse

Rating

Manufacturer Examples

RoHS Compliant (Lead Free)?

Yes

300 Watts

T6.3A,

250V

Bussmann: S506-6.3-R

Littelfuse: 218 06.3P

Bussmann: GDC-6.3A

Littelfuse: 218 06.3

A spare fuse is located in the power input module. Please refer to the fuse-replacement

appendix for more details on fuse ratings and for instructions on how to change the fuse.

Avoiding Electrical Shock and Fire-related Injury

Always use the grounded power supply cord set provided to connect the unit to a

grounded outlet (3-prong). This is required to protect you from injury in the event that

an electrical hazard occurs.

Do not disassemble the unit. The only user serviceable parts are the line fuse, the xenon

bulb, and filters. The line fuse is accessible from the outside of the unit. The bulb and the

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)

filters are accessible via separate panels on the side of the unit. Bulb replacement and

filter installation/removal are covered in separate sections of the manual and should only

be attempted with the power cord disconnected.

To prevent fire or shock hazard do not expose the unit to rain or moisture.

Electromagnetic Interference

To comply with FDA and CE electromagnetic immunity and interference standards; and to

reduce the electromagnetic coupling between this and other equipment in your lab always

use the type and length of interconnect cables provided with the unit for the interconnection

of one or more filter wheels and/or shutters, host computer via serial RS-232 or parallel

interface, or other equipment via TTL connections (see the TECHNICAL SPECIFICATIONS

appendix for more details).

Operational

Operate only in a location where there is a free flow of fresh air on all sides.

NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.

Do not operate the lamp in an orientation where the output of the CERMAX lamp faces

within 45°of straight upward. To do so may cause arc instability and the possibility of

damage to the front window of the CERMAX lamp. Note that the lamp output is towards

the heat sink mounted on the side of the cabinet.

Avoiding Physical Injury while Powered Up and Emitting Light

WARNING: DO NOT LOOK DIRECTLY INTO THE LIGHT GUIDE! The output

of the light aperture or the light guide should be directed into the microscope using the

appropriate adapters, directed away from anyone’s eyes, and not directed toward any

reflective surface.

INFRARED RADIATION: The infrared radiation (and ultraviolet radiation)

generated by this lamp can cause significant skin burns and eye damage.

THE SMARTSHUTTER IS NOT A SAFETY SHUTTER: The Lambda DG-

4PLUS/USB and DG-5PLUS/USB equipped with a Sutter Instrument

Smart

Shutter. The

SmartShutter is not intended to be a ‘safety shutter.’ A safety shutter usually closes

automatically in the event of a power failure and is designed with the primary goal of

ensuring that it will not allow any unintended exposure. For laser safety applications, a

shutter is normally designed so that no single component failure allows an unintended

exposure to the laser beam. The SmartShutter is intended for use in the controlling of

light in scientific and industrial applications. The SmartShutter was designed for high

performance and durability, but without certain features that would be desirable in a

safety shutter application.

EXPLOSION: High internal pressure exists in any xenon arc lamp.

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)

HIGH VOLTAGE: High ignition voltages, which exist inside the cabinet, can be

lethal.

WARNING: OZONE: The UV-enhanced (“full spectrum”) version of the xenon arc

bulb generates significant amounts of ozone, which is toxic. A Lambda-DG-Series system

installed with a full spectrum bulb must be connected to a ventilation or ozone-removing

system (also known as an “ozone eater”) for the evacuation of the ozone produced during

operation. Please contact Sutter Instrument (+1-415-883-0128 or info@sutter.com)

for further information. There are no ventilation requirements for the ozone-free bulb.

Avoiding Physical Injury and Equipment Damage while Replacing the Xenon Bulb

WARNING: Before removing the xenon arc lamp bulb housing assembly

from a Lambda LS or Lambda DG-Series system, make certain that the system is powered

down (single switch for the Lambda LS or both MAIN and LAMP switches for the Lambda

DG-4-series) and disconnect the power cord from the source. Wait at least half an hour for

the lamp to cool before removing the lamp bulb housing.

The original manufacturer of the xenon lamp and power supply used in the Lambda DG-

Series provides the additional safety information on the following page. It is intended to

amplify the information given above.

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)

PerkinElmer Optoelectronics

44370 Christy Street

Fremont CA 94538

Phone: 510-979-6500

FAX: 510-687-1152

SHORT ARC XENON LAMPS AND

SYSTEMS

Proper use and safe operating practices are the

responsibility of equipment manufacturers who

incorporate the lamp into equipment and users of

such lamps and equipment. The supplier of this

lamp provides information on its products and

associated hazards, but it assumes no

responsibility for after-sale operating and safety

practices. All lamps are under pressure and must

be handled with care. Take appropriate action

through baffles, light shields, interlock switches or

other safeguards to protect personnel from harm

due to operation and/or failure of the lamp.

SAFE OPERATING INSTRUCTIONS

Do not operate this lamp except in accordance

with proper operating instructions and within

recommended operating specifications. Direct

questions regarding lamp operation or safety to

your lamp supplier.

LAMP DISPOSAL

CERMAX lamps do not have reclaimable parts.

Before disposal, it is recommended to relieve a

lamp’s gas pressure by squeezing the tip-off with

pliers until the gas escapes. If gas pressure is not

relieved, care should be taken to discard the lamp

in a landfill and not an incinerator.

SAFETY HAZARDS

The operation of lamps involves one or more of

the following hazards. In the absence of safe

operating practices and precautions, any one of

these hazards could result in injury.

I. EXPLOSION - The lamps are filled with

xenon gas at very high pressure. Lamps must be

handled with the same care and caution given

any vessel containing these levels of pressure. A

hazard exists if the window or ceramic fractures

and may cause explosive mechanical failure.

Face shields or proper safety glasses are

recommended during all handling operations.

II. HIGH VOLTAGE - Ignition voltage of some

lamp models is very high and can be deadly. If

portions of the circuit are exposed, caution must

be used in setup and operation of the system.

The input power must be disconnected from the

power source before attempting any service to

the lamp.

III. INFRARED AND ULTRAVIOLET

RADIATION - Do not look directly at operating

lamps orb reflected light. Infrared and ultra

violet radiation generated by the lamp can cause

skin burns and permanent eye damage.

IV. OZONE - Some UV type lamps generate

ozone, a toxic gas, by virtue of the ultraviolet

radiation. A lamp which gives off ozone must be

operated in a well ventilated area..

V. HOT SURFACES - Portions of the lamp can

reach temperatures of several hundred degrees

centigrade and cause serious burns if touched

even after the lamp is turned off.

OPERATING HAZARDS

CERMAX® Lamps

Read the following instructions and take all necessary

precautions

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)

vii

TABLE OF CONTENTS

DISCLAIMER ......................................................................................................................................iii

SAFETY WARNINGS AND PRECAUTIONS...................................................................................iii

Electrical .................................................................................................................................................iii

Avoiding Electrical Shock and Fire-related Injury.............................................................................iii

Electromagnetic Interference ...............................................................................................................iv

Operational .............................................................................................................................................iv

Avoiding Physical Injury while Powered Up and Emitting Light ....................................................iv

Avoiding Physical Injury and Equipment Damage while Replacing the Xenon Bulb..................... v

1. GENERAL INFORMATION ...........................................................................................................1

1.1 Introduction.......................................................................................................................................1

1.1.1 How to Use this Manual............................................................................................................1

1.1.2 Technical Support ......................................................................................................................1

1.2 General Description..........................................................................................................................1

1.3 Differences between the Standard Lambda DG-4/5, the DG-4/5PLUS, and the DG-

4/5PLUS/USB..........................................................................................................................................2

1.4 Lambda DG-4PLUS/USB and Lambda DG-5PLUS/USB............................................................3

1.4.1 Systems........................................................................................................................................3

2. INSTALLATION ..............................................................................................................................5

2.1 Unpacking..........................................................................................................................................5

2.2 Getting Started..................................................................................................................................5

2.3 Light Guide & Optical Holder Installation ....................................................................................8

2.3.1 Liquid Light Guide.....................................................................................................................8

2.3.2 Liquid Light Guide Installation................................................................................................8

2.3.3 Optical Holder ............................................................................................................................9

2.4 Loading Filters ................................................................................................................................10

2.4.1 Filter Orientation.....................................................................................................................10

2.4.2 Loading Filters .........................................................................................................................10

2.4.3 Selection of a Filter Position...................................................................................................11

2.4.4 Blanking Discs ..........................................................................................................................11

2.4.5 Neutral Density Filters............................................................................................................11

2.4.6 Filter Holder Installation ........................................................................................................11

3. OPERATIONS ................................................................................................................................13

3.1 Control Electronics .........................................................................................................................13

3.2 Preparation and Precautions.........................................................................................................13

3.3 Power-Up Sequence........................................................................................................................13

3.4 Top Panel Controls .........................................................................................................................14

3.4.1 Keypad.......................................................................................................................................14

3.5 Electrical Connections....................................................................................................................15

3.5.1 Parallel Port..............................................................................................................................16

3.5.2 Serial Port .................................................................................................................................16

3.5.3 USB Port...................................................................................................................................16

3.5.4 Filter Value Out .......................................................................................................................17

3.5.5 SHUTTER ................................................................................................................................17

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)

viii

3.5.6 STROBE....................................................................................................................................17

3.5.7 DAC............................................................................................................................................17

3.5.8 Line Power................................................................................................................................17

3.6 Fuse ..................................................................................................................................................17

3.7 Modes of Operation (LOCAL)........................................................................................................17

3.7.1 General Information ................................................................................................................17

3.7.2 Selecting an Operational Mode: The MODE Key.................................................................18

3.7.3 Move on Command Mode........................................................................................................19

3.7.4 Move on Sync Pulse Mode.......................................................................................................20

3.7.5 Move on Strobe Pulse Mode....................................................................................................21

3.7.6 Move on Sync, Inhibited by the Strobe Line Mode ..............................................................22

3.7.7 Edit a Filter Definition Mode..................................................................................................23

3.7.7.1 General Information .........................................................................................................23

3.7.7.2 Filter Numbers ..................................................................................................................24

3.7.7.3 Attenuation ........................................................................................................................25

3.7.7.4 Programming a Filter Number (“Logical Filter”)\........................................................26

3.7.7.5 Manual Adjustment of Attenuation ................................................................................27

3.7.8 Setup Ring Buffer Mode ..........................................................................................................28

3.7.8.1 General Information .........................................................................................................28

3.7.8.2 Storing the FILTER NUMBER Sequence .....................................................................29

3.7.8.3 Viewing an Existing FILTER NUMBER Sequence ......................................................30

3.7.8.4 Running the RING BUFFER Sequence .........................................................................31

3.7.9 Turbo-Blanking Mode..............................................................................................................32

3.7.10 Display the First Four Filter Values Mode .........................................................................33

4. EXTERNAL CONTROL OPERATIONS......................................................................................35

4.1 Parallel Interface.............................................................................................................................46

4.1.1 Connecting to the Lambda DG-4 parallel port .....................................................................46

4.1.2 Input Lines................................................................................................................................46

4.1.3 Command Codes.......................................................................................................................47

4.1.4 Busy Line ..................................................................................................................................47

4.1.5 Interface Modes ........................................................................................................................48

4.1.6 Controlling the Lambda DG-4 from a Computer’s Parallel (Printer) Port.......................48

4.1.7 Reading the Status of the Lambda DG-4...............................................................................50

4.1.8 Shutter Control ........................................................................................................................52

4.2 Serial Interface................................................................................................................................52

4.2.1 Connecting to the Lambda DG-Series Serial Input .............................................................52

4.2.2 Controlling the Lambda DG-4 from a Computer’s Serial Port...........................................53

4.2.3 Serial Port Command Echo ....................................................................................................54

4.2.4 Serial Port Command Completion Indicator ........................................................................54

4.2.5 Reading the Status of the Lambda DG-4...............................................................................54

5. MAINTENANCE............................................................................................................................59

5.1 Changing the Xenon Arc Bulb.......................................................................................................59

5.1.1 Bulb Assembly ..........................................................................................................................59

5.1.2 Removing the Lamp Assembly from the System..................................................................60

5.1.3 Bulb Replacement ....................................................................................................................61

5.1.3.1 Removing the Existing Bulb ............................................................................................61

5.1.3.2 Installing the New Bulb....................................................................................................61

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