Reichert Univar Manuel utilisateur
Reichert Univar
Manual
Translated from the 11/1975 German language edition,
with slight modifications.
William R. Porter
San Marcos CA USA
2017
v 1.4
Notes
This is a very slightly-modified, new (2017) American English translation of the 1975
German-language version of the Reichert Univar microscope operating manual.
In a very few places, the text has been rewritten slightly for clarity. Any mistakes are
probably mine. No other Univar manuals or literature were used (nor indeed available) in the
production of this one. This manual was translated purely for the use of myself; it is being
made available to the public, free of charge, for their own use and enjoyment, with all the
usual disclaimers and the understanding that they do so at their own risk, etc., etc.
It was composed with the help of SoftMaker Presentations, Google Translate, and an
assortment of utilities, all on Ubuntu Linux.
The typeface is DejaVu Sans Condensed.
Table of contents
A) Setup and assembly.................………………..................................................................... A1
B) Univar transmitted-light setup
Transmitted-light brightfield microscopy……................................................................. B1
Transmitted-light darkfield microscopy…………............................................................. B3
Transmitted-light phase and anoptral contrast……....................................................…..B5
Transmitted-light microscope with polarized light …....................................................…B7
Transmitted-light interference contrast……………....……….………………….......................B9
Transmitted-light brightfield fluorescence…………………………………..................……….. B11
Transmitted-light darkfield fluorescence……………………………….……............…………… B13
Simultaneous contrast fluorescence with transmitted-light...……………………….....……. B15
Simultaneous darkfield fluorescence with transmitted-light ………......………............…. B17
C) Univar epifluorescence attachment
Immunofluorescence using epifluorescence ............……………….………………………...… C1
Conventional fluorescence and quinacrine using epifluorescence ......................…..….. C3
Detection of aromatic amines using epifluorescence ..........………………………..……...... C5
Mixed illumination: epifluorescence + transmitted-light darkfield, &
epifluorescence + transmitted-light darkfield fluorescence ...………….........…… C7
Mixed illumination: epifluorescence + transmitted-light phase and anoptral contrast .. C9
Mixed illumination: epifluorescence + transmitted-light interference contrast ..……..... C11
Mixed illumination: epifluorescence + transmitted-light polarization …………...……...... C13
D) Mirror & lamp houses and connections
Mirror house 4 ........…...…………………………………………………………………………..........D1
Mirror house 2 ........……...………………………………………………………………………..........D3
Lamp house 10 .....………………………………………………………………………………........... D5
Lamp house 25 .....………………………………………………………………………………........... D7
Lamp house 50 .....………………………………………………………………………………........... D9
Power supply housing .………………………………………………………….……………….......... D11
Power supply for 100W 12-volt halogen lamp .......…………………………….…………....…..D11
Power supply for HBO 200W/4 or CS 200W/4 mercury-vapor high-pressure lamp ......... D12
Power supply for CSI 250W/1 metal-halide short-arc lamp ....………………………….….... D12
Intermediate transformer...…………………………………………………………………………..... D13
Power supply for HBO 200W/2 mercury-vapor high-pressure lamp .….......……….……... D13
Power supply for XBO 450W xenon lamp .……………….....……………………...…………..... D14
Remote control device for the control unit to the XBO 450W xenon lamp .....…........…..D14
(E) Trimatic camera system ………………………………………………………………………............ E1
Installation and connection of the Trimatic camera system ………………………………….. E3
Operation ………………………………………………………………………………...………............ E5
Special cases:
Point measurement ..……………………………………………………………………..........E7
Fluor button ….....……..…………………...…………………………………………….......... E7
Double exposure .……………………………………………………………………...............E7
Half-frame photograph ...…………….…………………………………………………......... E7
Extension factors …………..........……………………………………………………………............ E8
Interruption of exposure ..……………………………..…………………………………………........E8
Lock Gate ………………………………………………………………………………………............... E8
Magnification in the film plane ..……………………………………………………………...…...... E9
Remote control ...………………………………………………………………………………............. E9
Micro Flash Terminal ...………………………………………………………………………...….........E9
Camera with lnternational camera back, format 4” x 5”..…….…………………………........ E10
Camera with Polaroid Pack film cassette, style 3 1/4” x 4 1/4" ..……………………………..E10
Automatic camera for the 24 x 36mm format ..……………………………………………….... E11
F) General notes
Observation tube ...…………………………………….………………………………………............ F1
Rotating stage No. 28 ............…………………………………………………………………..........F1
Coarse drive stop ....……………………………………………………………...……………............ F1
Condenser, coarse and fine adjustments ....…………………………………………………....... F1
Transmitted-light objectives ....…………………………………………………………………........ F2
Wide-field plan compensation eyepieces.....……………………………………………….…...... F2
Relay system .....……………………………………………………………………………….............. F3
Magnification changer................................…………...………………………………………...... F3
Slider slot........…………………………………………………………………………………...............F3
Measuring micrometers.................................................................................................. F3
Counting grids................................................................................................................ F3
Half-frame panels........................................................................................................... F3
Projection attachment ..…………………………………………………………………………..........F4
Automatic zoom & magnification indicator ……….……………………………………………... F4
Care of the microscope ………………………………………………………………………............. F5
Please note:
The mirror house 4 was used in the illustrations of the different working methods. If the mirror house 2 is
installed, the workflow remains the same, but references to non-existent knobs should be ignored. There
are left and right and upper and lower knobs, as well as the coaxial knobs for filters.
Erecting and assembling
Setting up:
Three handles are screwed into the base plate, and are used to lift the
microscope stand. The tripod weighs approx. 55 kg (121 lb) and should
be lifted by 2 people. The desk on which the Univar is positioned must
be stable, since the microscope with all of its associated gear can
weigh up to 120 kg (264 lb).The countertop must be hard and flat, so
that an 8mm air gap on the underside of the large lamps houses is
ensured for proper air circulation.
Remove the protective transport pieces. The necessary hex wrench to
unscrew the pieces is included.
a) Remove the red plate covering the projection prism by unscrewing
the two allen screws.
b) Remove the red protector in the relay housing.
c) Remove the red locking screw for the beamsplitter prism and
replace with the supplied 32mm long screw.
d) Remove the red protector on the focus rack, after unscrewing the
hex bolt and the slotted screw. Withdraw the protector after the rack is
slightly raised. Replace the slotted screw.
e) Do not move the coarse feed before the assembly of the stage
support.
A1
Assembly of the stage:
The stand has a dovetail mount and the stage support has a mating dovetail.
There are cutouts on each dovetail that allow the two to be fastened together.
First, unscrew the large screw with a coin slot, ➂, near the top of the stage
dovetail. Then, push the stage support onto the stand dovetail (you may have
to move the stage up and down to accomplish this using the recesses). Once
together, lower the stage support until the focusing lever (the end of which is
visible through the opening ➀) is centered behind the threaded hole for screw
➂. The table support is held in this position. Run the screw into the threaded
hole in the release lever.
Lower the stage support until the upper edge of the index ➁ is level with the
upper surface of the stand dovetail mount. Clamp the locking lever ➃. This is
the correct setting for the coarse-drive stop for regular transmitted-light
preparations on 1mm thick microscope slides.
Assembly of the condenser holder:
Turn the condenser turret to the illustrated position; the screw-in condensers
should be oriented in a front-back direction. Run the condenser mount with the
condenser coarse-feed to the lower stop position. Hang the condenser turret
transverse pin into the v-shaped notch on top of the stand's condenser mount
by tilting it back slightly, hanging by the pin, and letting it down gently. Then
clamp the condenser carrier to the mount by tightening the knurled control
knob ➄ in a counter-clockwise direction a partial turn.
Assembling the nosepiece carrier and the objectives:
Screw in objectives into the optics carrier (the 10x lens is screwed into the
threaded hole marked with a dot.) The other lenses are installed so that the
objectives are higher magnification when the rotation of the objective
nosepiece is in a clockwise direction. With the stage in its lowest position (with
coarse focus), insert the optics carrier into the guide on the microscope stand
up to the stop and clamp with clamping screw ➅.
Magnification changer (if available):
Remove the cap from the opening on the stand. Turn the knob of the
magnification changer position to 2.5 (arrow). In this position you can slide in
the the magnification changer on its dovetail until it stops.
Phase-ring knob (if available):
Remove the cap from the opening of the stand. Insert the phase-ring assembly
by pressing on the adjustment knob to slide it onto the dovetail, and clamp
with the captive cap-head screw.
A2
A3
Attaching the mirror housing:
Remove the back of the mirror housing, after loosening the four
corner fasteners by turning each fastener counterclockwise 1/4
turn.
Fasten the housing to the microscope stand with the captive
allen screw inside the housing (about a third of the way down in
the center) using a long allen wrench or other hex key tool. Do
this with care so no prism or filter is damaged.
Remove the protective covers and foam blocks for the sliding
mirror, rotating prism and oscillating mirror. Do not touch optical
parts with your fingers.
Insert the filter holder with IR-blocking filters in the filter holder
on the rear. The filter holder top is marked either X or H .
The filter holder X is used for working with the XBO 450W Xenon
lamp; for the HBO 200W mercury-vapor pressure lamp, use filter
holder H.
Replace the back panel of the mirror housing on the orientation
pin located above, and secure it with the four captive corner
fasteners by tightening them about 1/4-turn clockwise.
Be careful that the filters are not damaged.
Note! If the rear panel heat-blocking filters are not in place, do
not operate the xenon or mercury-vapor lamps, since the prisms
will be damaged by the heat from the lamp.
Mounting the lamp housings:
Remove the protective caps on the mirror housing and attach the
lamp housings with the screws at the back. See the description
of the lamp housing and the electrical connection on the lighting
equipment.
Zoom lighting:
The connection cable for the automatic zoom lighting is
connected between the microscope base and the power supply
for the 100W low-voltage halogen lamps.
A4
Projection attachment:
The projection tower is placed on the microscope
stand, with the cylindrical light inlet over the opening
of the stand.
Carrying handles:
The three carrying handles can be unscrewed from the
base of the microscope stand. The threaded holes are
provided with screw plugs.
Hand pads:
The magnetic hand rests are pushed on to the stand.
Eyepieces:
The eyepieces are inserted into the eyepiece tubes so
that the orientation pins engage in the grooves.
Photo Setup: The assembly of the photo setup is
described elsewhere.
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