PSI Polypen Aqua Manuel utilisateur

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Manual Version: 2021/01

www.psi.cz

This document and its parts can be copied or provided to a third party only with the express

permission of PSI.

The contents of this manual have been verified to correspond to the specifications of the device.

However, deviations cannot be ruled out. Therefore, a complete correspondence between the

manual and the real device cannot be guaranteed. The information in this manual is regularly

checked, and corrections may be made in subsequent versions.

The visualizations shown in this manual are only illustrative.

This manual is an integral part of the purchase and delivery of equipment and its accessories

and both Parties must abide by it.

 
 
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TABLE OF CONTENT 
 
1Warnings and Safety Precautions ...............................................................................4 
2Technical specifications .............................................................................................5 
3General Information..................................................................................................6 
4Components of the PolyPen-Aqua Device...................................................................7 
5Description of the PolyPen-Aqua Device.....................................................................8 
6Care and Maintenance...............................................................................................9 
7Operation Instruction .............................................................................................. 10 
7.1 Calibration.................................................................................................................... 10 
7.2 Measurement............................................................................................................... 12 
7.3 Main Menu................................................................................................................... 13 
 Device.................................................................................................................. 13 
 Data..................................................................................................................... 13 
 Options................................................................................................................ 14 
8SpectraPen Software ............................................................................................... 19 
8.1 Driver Installation......................................................................................................... 19 
8.2 Software Installation.................................................................................................... 21 
8.3 SpectraPen Software Menu.......................................................................................... 24 
8.4 Data Transfer and Visualization................................................................................... 25 
8.5 Online Control .............................................................................................................. 30 
8.6 Firmware Update ......................................................................................................... 32 
9GPS Module ............................................................................................................ 33 
9.1 GPS / PolyPen-Aqua Operation .................................................................................... 33 
10 How Can I Program My Own Index In The PolyPen-Aqua PA 210 ............................... 35 
10.1 Function Description..................................................................................................... 36 
11 Warranty Terms and Conditions............................................................................... 38 
12 Troubleshooting and Customer Support ................................................................... 39 
 
 

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1WARNINGS AND SAFETY PRECAUTIONS

Read this manual carefully before operating the device. If you are not sure about anything in

the manual, contact the manufacturer for clarification.

By accepting the device, the customer agrees to follow the instructions in this

guide.

Always follow corresponding manuals while working with the PolyPen-Aqua device or doing the

maintenance.

It is forbidden to interfere with the hardware or software of the PolyPen-Aqua device in any

way without previous agreement with the manufacturer.

The following table presents basic highlight symbols used in this manual:

Symbol

Description

Important information, read carefully.

Complementary and additional information.

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2TECHNICAL SPECIFICATIONS

Spectrum measurement

Spectral range

340 nm - 790 nm

Spectral response half width

8 nm

Spectral straylight

-30 dB

Scanning speed

About 100 ms

Dynamic range

High gain: 1:4,300

Low gain: 1:13,000

Size of aperture

7 mm

Light source

Type

Xenon incandescent lamp

Spectral range

340-790 nm

Data storage and transfer

Internal memory capacity

Up to 16 Mbit

Internal data logging

Up to 4,000 measurements

Data transfer

USB cable

PC software

SpectraPen 1.1 (Windows 7 and higher)

Battery

Type

Li-Ion rechargeable battery

Capacity

2600 mAh

Max. charging current

0.5 A

Charging

Via USB port - PC, power bank, USB charger, etc.

Battery life

48 hours typical with full operation

Low battery indicator

Other

Sample holder

4 ml cuvette

Display

Touchscreen 240 x 320 pixel; 65535 colors

Built in GPS module

Ultra-high sensitivity down to -165dBm

High accuracy of <1.5 m in 50% of trials

Size

150 x 75 x 40 mm

Weight

300 g

Operating conditions

Temperature: 0 to +55 °C

Relative humidity: 0 to 95 % (non-condensing)

Storage conditions

Temperature: -10 to +60 °C

Relative humidity: 0 to 95 % (non-condensing)

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3GENERAL INFORMATION

PolyPen-Aqua PA 210 is a portable, handheld and reasonably priced spectrophotometer

intended for measuring spectra in suspensions. It can be used favorably in a wide range of

applications –biotechnology, limnology, ecology, molecular biology, chemistry, forensic science

etc. The device is convenient both for standard laboratory work and for field work, well using

its inbuilt GPS unit. The PolyPen-Aqua measures absorbance and transmittance spectra based

on a single beam method; detection range is 380 –790 nm. The device is equipped with an

internal light source - Xenon incandescent lamp.

PolyPen-Aqua can be used as an autonomous instrument. It is powered by an inbuilt Li-Ion

rechargeable battery and thus it does not require a PC or any other bulky accessory for its

operation. Measured spectra are instantly displayed in graphs or data sheets on the device

touch screen. Full spectra information is stored in the device memory for later re-analysis in a

PC. The important part of the PA 210 package is the control software. It allows user-friendly,

online control of the device as well as measured data saving, uploading and further processing

in a PC. Definition of custom calculated parameters based on measured spectra is also possible.

PolyPen-Aqua PA 210 measures:

•Whole absorbance and transmittance spectra:

Transmittance –calculated using the following formula

T= I/I0

Where: I0is reference light intensity

Iis measured light intensity

Absorbance –calculated data using the following formula

A= log(I0/I)

Where: I0is reference light intensity

Iis measured light intensity

•Optical density (OD) at 600 nm, 680 nm, 735 nm and 750 nm.

Optical density –calculated data using the following formula

OD = -log(I/I0)

Where: I0is reference light intensity

Iis measured light intensity

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4COMPONENTS OF THE POLYPEN-AQUA DEVICE

Carefully unpack the carton. You should have received the following items, as described in table

below:

1. PolyPen-Aqua PA 210

2. Stylus

3. Carrying Case

4. Textile Strap for Comfortable Wearing

5. This Operation Manual (PDF file on a USB flash disc)

6. USB flash disc with SpectraPen software and driver

7. USB Communication Module

8. Other Accessories or Optional Features (according to your specific order)

For USB connection you need to have the USB driver installed in your PC. You

find the driver on the installation disk (USB driver folder).

If any item is missing, please, contact the manufacturer. Also check the carton

for any visible external damage. If you find any damage, notify the carrier and

the manufacturer immediately. The carton and all packing materials should

be retained for inspection by the carrier or insurer.

For customer support, please write to: support@psi.cz

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5DESCRIPTION OF THE POLYPEN-AQUA DEVICE

PolyPen-Aqua is a compact, durable and lightweight device operated by single Turn ON/OFF

button and touch screen (Fig. 1 PolyPen-Aqua device.Fig. 1). It features an innovative user

interface with an integral computer, a full color touch screen display and an in-built light source.

The sample is measured in a plastic cuvette inserted into an optical head and covered by a top

lid.

Fig. 1 PolyPen-Aqua device.

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6CARE AND MAINTENANCE

•Never submerge the device in water!

•The device should not come in contact with any organic solvents, strong acids or bases.

•Battery life is approximately 48 hours when the PolyPen-Aqua is operated continuously.

•Use the provided stylus for the touch-screen operation. Avoid usage of any sharp objects.

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7OPERATION INSTRUCTION

This chapter provides instructions on an initial set-up operation of the PolyPen-Aqua PA 210

device as well as detailed, step-by-step description of a measurement procedure.

To switch the PolyPen-Aqua ON, press the POWER BUTTON until the device logo shows up.

Follow next steps to perform the measurements. In case the device battery is not charged,

connect the PolyPen with USB cable via USB port to the PC and charge the batteries.

7.1 Calibration

Calibration of the PolyPen-Aqua device MUST be done prior measurement after the turning ON

of the device or it should be periodically repeated upon certain time period of the device

operation.

For calibration of device use plastic cuvette with clear media.

Please follow the instructions below for device calibration:

•Use the standard 4 ml cuvette.

•Clean the cuvette with distilled water and paper tissue.

•As a calibration standard use cultivation medium (BBM, BG11 etc.) or distilled water.

•Put cuvette with medium (minimal volume 2 ml) into the PolyPen-Aqua device and

close the cover.

•In the main window go to Calibrate > press OK (Fig. 2) and wait until the calibration

step is completed.

Fig. 2 Calibration of device.

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