PendoTECH PM2 Manuel utilisateur

www.pendotech.com Tel: +1-609-799-2299

PENDOTECH® PM2

PHOTOMETER FOR UV/VIS

ABSORBANCE AND TURBIDITY

MEASUREMENTS

USER’S MANUAL

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

Measurements User’s Manual Revision 0

www.pendotech.com Tel: +1-609-799-2299

Safety Instructions

Definition of Equipment and Documentation Symbols and Designations

WARNING: POTENTIAL FOR PERSONAL INJURY

CAUTION: Possible instrument damage or malfunction

NOTE: Important operating information

On the transmitter or in this manual text indicates Caution and/or other possible hazards, including the risk of electric

shock (refer to accompanying documents).

Each prospective user must test the measurement unit for its proposed application to determine its suitability for

the purpose intended prior to incorporating the sensor to any process or application. Proper safeguards must be

put into place for the process in which the unit is used.

The functioning and operational safety of the product can only be ensured if the user observes the usual safety

precautions as well as the specific safety guidelines stated in these operating guidelines:

The PM2 Photometer must only be operated under

the specified operating conditions.

The PM2 Photometer should be installed and

operated only by personnel familiar with the

Photometer and who are qualified for such work.

Before connecting the device to a supply unit, ensure

its output voltage cannot exceed 48 VDC. Do not use

alternating current or a main power supply!

Warning: Only use a power supply with double

insulation or reinforced insulation to supply this

product.

Warning: Do not submerge this product. Protect the

product before cleaning with any liquids by covering

openings that expose the internal components.

Install equipment as specified in this instruction

manual. Follow appropriate local and national codes.

Power supply must be disconnected before servicing.

The safety of the user may be endangered if the

instrument:

•is visibly damaged

•no longer operates as specified

•has been damaged in transport

Process upsets: because process and safety

conditions may depend on the consistent operation

of this transmitter, provide appropriate means to

maintain operation during sensor cleaning,

replacement, or sensor or instrument calibration.

Except for routine maintenance and cleaning procedures,

the PM2 Photometer must not be tampered with or altered

in any manner.

Maintenance and repair work must only be carried out by

PendoTECH.

METTLER TOLEDO/PendoTECH accepts no

responsibility for damage caused by unauthorized

modifications to the Photometer.

Follow all warnings, cautions, and instructions indicated

on and supplied with this product.

Electrical installation must be per the National Electrical

Code and/or any other applicable national or local codes.

If this equipment is used in a manner not specified by the

manufacturer, its protection against hazards may be

impaired.

Relay action: the PM2 Photometer relays will always de-

energize on the loss of power, equivalent to a normal

state, regardless of the relay state setting for powered

operation. Configure any control system using these

relays with fail-safe logic accordingly.

The terminals are suitable for single wires / flexible leads

0.2 mm2 to 1.5 mm2 (AWG 16 – 24).

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

Measurements User’s Manual Revision 0

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Optical Safety

Warning: The photometer can emit light in the range

of 200 nm – 1100 nm. Hazardous UV and IR

radiation are emitted from the unit. Never directly

stare into the source/return ports on the photometer

or the fiber optic cables, as this can cause eye

damage. The viewer-related risk depends on how the

users install and use the product.

The photometer should always be powered off when

handling the fiber optic cables. Protective eyewear

must be worn if you must handle the fibers when the

unit is powered on.

Ensure that both ends of the fiber optic cables

(photometer and flow cell connections) are properly

secured (firmly hand-tight) before powering on the

photometer.

In the event of product failure, do not attempt to open the

unit or replace the LED. There are no user-serviceable

parts.

Fig 1: Warning label, PM2, Fiber cable.

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

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Contents

Safety Instructions.................................................................................................................................................................. 1

1 Disclaimer ............................................................................................................................................................................ 4

2 Introduction and Unpacking ............................................................................................................................................... 4

2.1 Identify the following components............................................................................................................................... 4

3 Flow Cell and Flow Cell Stand ............................................................................................................................................. 5

3.1 Flow Cells ...................................................................................................................................................................... 5

3.2 Optical Couplers............................................................................................................................................................ 5

4 Unit Overview...................................................................................................................................................................... 6

5 Hardware installation.......................................................................................................................................................... 7

5.1 Environmental............................................................................................................................................................... 7

5.2 Physical.......................................................................................................................................................................... 7

5.3 Panel Display and Buttons ............................................................................................................................................ 7

............................................................................................................................................................................................ 7

5.4 Alarms ........................................................................................................................................................................... 8

5.5 Configuration of Unit for Laboratory or Panel Mount Installations ............................................................................. 9

5.6 Optical ........................................................................................................................................................................... 9

5.7 Electrical...................................................................................................................................................................... 10

6 Introduction to Absorbance Monitoring .......................................................................................................................... 11

6.1 Theory of Operation.................................................................................................................................................... 11

6.2 LED-Based Absorbance ............................................................................................................................................... 12

7 Glossary and definitions.................................................................................................................................................... 13

8 Instrument Control............................................................................................................................................................ 14

8.1 Zero ....................................................................................................................................................................... 14

9 Absorbance Operations ..................................................................................................................................................... 14

10 System Specification ....................................................................................................................................................... 15

10.1 Optical Configuration................................................................................................................................................ 15

10.2 PM2 Specifications.................................................................................................................................................... 15

10.3 Service Information................................................................................................................................................... 15

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

Measurements User’s Manual Revision 0

www.pendotech.com Tel: +1-609-799-2299

1 Disclaimer

in any form or by any means, whether electronic, mechanical, by photocopying or otherwise, without the written consent of

PendoTECH.

The information in this User Guide is believed to be accurate and reliable for the use and operation of the monitor;

however, PendoTECH assumes no responsibility for the use of this product except for what is covered in the Limited

Warranty and Terms and Conditions of Sale.

NOTE: “NOTE” is used to notify the user of installation or operation information that is important but not hazard-related.

2 Introduction and Unpacking

The PendoTECH UV PM2 Photometer is a versatile measuring and monitoring device that allows users to measure

absorbance and turbidity in bioprocess fluid streams. The device uses a collimated beam of light that passes through a

sample with a defined path length to determine the absorbance of the sample. The photometer can measure UV

absorbance at 260nm, 280nm, or 300nm to identify the presence or absence of a molecule of interest and at 880nm to

measure turbidity and filter performance.

The photometer also functions as a transmitter with a 4-20mA output scaled 0-3 AU, making it easy to integrate with other

monitoring and control systems. The photometer is available in a dual-wavelength version, which allows users to make

two measurements of multiple wavelengths simultaneously in the same sample.

To make measurements in-line, the photometer uses PendoTECH Single Use UV/Turbidity Flow Cells, which contain a

special silica glass lens and compartments for optical couplers. The flow cells can be repeatedly cleaned and reused but

are designed for single use. PendoTECH Flow Cell Stand is an accessory that can be used to house or mount these flow

cells as an alternative to optical couplers. The stand is recommended for improved usability and accessibility when

changing flow cells and for blocking out ambient light in turbidity applications.

Overall, the PendoTECH UV PM2 Photometer and Single Use Flow Cells provide a reliable and convenient solution for

measuring and monitoring bioprocess fluid streams.

2.1 Identify the following components

Note: All the parts listed below are to be ordered individually.

Photometer

PM2

Flow cell Large Stand Flow cell Small Stand

Calibration Kit

Coupler(s)

Fiber Optic Cable

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

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3 Flow Cell and Flow Cell Stand

Note: For Turbidity, a Flow Cell stand is required to block ambient light at 880nm from interfering with the measurement

3.1 Flow Cells

The combination of the UV PM2 photometer and the PendoTECH Single Use Flow Cells allows the measurements to be

made in line. The flow cells contain a special silica glass window and compartments to insert optical couplers, which

attach to the fiber optic cables from the photometer. The flow cells can also be used with PendoTECH’s Flow Cell Stand

with integral couplers in place of the optical couplers to connect the fiber optic cables. The flow cell stand is optional but

recommended because it blocks out ambient light that could otherwise affect the absorbance readings. Additionally, the

flow cell stand improves accessibility and makes it easier to change flow cells. Although designed for single use, the flow

cells may be repeatedly cleaned and reused.

3.2 Optical Couplers

SMA905 connectors of the

connection of the fiber optics

Flow Cell stand cover to secure

the flow cell and blocks ambient

light

Flow Cell stand for 6.5cm flow cell

Material #: 30849504

Flow Cell stand for 1cm flow cell

Material #: 30849505

6.5cm Single-Use Turbidity Clow

Cell

Flow Cell with tubing installed

Optical Couplers installed to the

Flow Cell

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

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4 Unit Overview

Note: For single channel units, only pins 1 and 2 are used. For dual channel units, pins 1 and 2 correspond to the photometer’s first

wavelength (lower), and pins 3 and 4 correspond to the second wavelength (higher).

4-20 mA +

RS485-A

ZERO -

4-20 mA -

RS485-B

ALARM +

4-20 mA +

GND

ALARM -

4-20 mA -

12-48 VDC

POWER

4-20 mA = 0-3 AU

RS485 Shield

ZERO +

Fig 2: PM2 (Photometer) dimensions in mm.

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

Measurements User’s Manual Revision 0

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5 Hardware installation

Please follow the outlined sections below as a guideline for installing the photometer and associated accessories. These

guidelines apply for specific application installations or, in the case of OEM component installation onto a suitable panel.

5.1 Environmental

The photometer is strongly recommended to be installed in a clean, dry area where the ambient temperature does not

exceed 115F/46C. Systems mounted in enclosures can be purged with clean, dry, oil-free air (or nitrogen) to dissipate

heat.

5.2 Physical

For permanent installations

Bolt the photometer, back panel, or enclosure (as appropriate) in place using mounting screws. The location should be

secure, rigid, and strong enough to support the weight of the installed system.

For laboratory use

Ensure sufficient space on the bench or tabletop to accommodate the hardware supplied.

For panel mount use

Ensure sufficient space on the bench or tabletop to accommodate the hardware supplied.

For all installations

Run the fiber optic cable(s) through conduit or other appropriate protective measures as desired. Refer to Section 5.3

below for optical component installation instructions.

Run required electrical wiring for instrument power and output signals (4-20mA). Review Section 5.4 below before making

electrical connections.

5.3 Panel Display and Buttons

DO NOT SUPPLY POWER TO THE UNIT UNTIL TOLD TO DO SO IN THE INSTRUMENT SETUP

AND INITIALIZATION PROCEDURE IN SECTION 5

Reporting the current

measured absorbance in

Absorbance Units [AU].

Wavelength of the used

LED displayed.

Overall status of the device. During

error-free operation, the textbox will

state “OK” on a green background.

This status is shown when a “Tare”

button is pressed for 5

seconds. Once it is tared, the

absorbance will be set to 0.00 AU.

Display

Wakeup/Backlight

On.

Tare button

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

Measurements User’s Manual Revision 0

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5.4 Alarms

Refer to the PM2 photometer’s Display, indicating an alarm condition. For the 4-20mA outputs will indicate alarms (going to high-level).

Alarm Activator

Cause

Solution

The measurement detector is

saturated at 100%.

Photometer outputting invalid

absorbance values due to bad tare.

Re-tare flow cell with background

solution or air.

The reference detector is saturated

at 100%.

Extreme amount of ambient light

entering flow cell/measure detector.

Use flow cell holder to block ambient

light from entering flow cell; ensure

fiber optic cable connections are firmly

hand tight.

The reference light and dark signals

are too close to each other.

LED is dead.

Send back to PendoTECH for repair.

On errors the device enters the

alarm mode. (See section alarms).

The screen signals -NA- (Not

applicable) on a red background in

the [AU] textbox.

The status textbox states “ALARM”

and changes background to red.

Reporting the current measured absorbance

in Absorbance Units [AU]. Left side for

channel 1 right side for channel 2.

Wavelength(s) of the used

LED displayed.

Overall status of the device. During

error-free operation, the textbox will

state “OK” on a green background.

This status is shown when a “Tare”

button is pressed for 5

seconds. Once tared, set the

absorbance to 0.00 AU for both

channels.

The screen signals -NA- (Not

applicable) on a red background in

the affected AU channels.

The status textbox states “ALARM”

and changes background to red.

PendoTECH PM2 Photometer for UV Absorbance and Turbidity

Measurements User’s Manual Revision 0

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5.5 Configuration of Unit for Laboratory or Panel Mount Installations

PM2 has dual functionality to operate as a laboratory benchtop unit or panel mount unit. By default the configuration

switch is in the Lposition or laboratory unit. In this configuration the power connector and the 8-pin circular input/output

connector are active. To change to the panel mount configuration where power, input/output functions are on the screw

terminal connector, move the switch to the Pposition as shown.

5.6 Optical

1. Remove the protective caps from the SMA-905 connectors on the fiber optic cables and the photometer.

2. Clean the fiber ends with spectroscopic-grade isopropyl alcohol (IPA, also known as 2-propanol) or methanol

using a lint-free cotton swab or non-abrasive task wipe. Spectroscopic grade acetone can also be used, but

separation of cotton from the swab might occur as the binding agent dissolves.

3. Properly mount the flow cell stand/holder (if applicable to system). The flow cell stand can be set directly flat on

a lab bench or should be properly wall/panel mounted. Proper mounting of the flow cell stand facilitates the

pass through of bubbles/air pockets in the fluid stream, which if trapped in the flow cell, can degrade system

performance.

Fiber optic connections

Fig 4: Complete system setup showing measure unit/PM 2

Photometer, flow cell stand/holder, and fiber optic cables

Fig 3: Front view of the PM2 Photometer with fiber optic cables for

source and return.

Flow Cell

Stand

Fiber Optic

cables

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