Optika B-500 MET Manuel utilisateur

OPTIKA MICROSCOPES - ITALY

www.optikamicroscopes.com - [email protected]

Ver. 1.0.0

B-500 MET

OPERATION MANUAL

GUIDA UTENTE

MANUAL DE INSTRUCCIONES

BEDIENUNGSANLEITUNG

Page 2

INDEX

SAFETY GUIDELINES page 3

1.0 DESCRIPTION page 4

2.0 INTRODUCTION page 6

3.0 UNPACKING AND ASSEMBLY page 6

4.0 USING THE MICROSCOPE page 8

5.0 MAINTENANCE page 11

6.0 ELECTRICAL SPECIFICATIONS page 11

7.0 RECOVERY AND RECYCLING page 12

Page 3

SAFETY GUIDELINES

This microscope is a scientic precision instrument designed to last for many years with a minimum

of maintenance. It is built to high optical and mechanical standards and to withstand daily use.

Optika reminds you that this manual contains important information on safety and maintenance, and

that it must therefore be made accessible to the instrument users.

Optika declines any responsibility deriving from instrument uses that do not comply with this ma-

nual.

Safety guidelines

This manual contains important information and warnings regarding safety about installation,

use and maintenance of the microscope. Please read this manual carefully before using the

equipment. To ensure safe use, the user must read and follow all instructions in this manual.

OPTIKA products are designed for safe use in normal operating conditions. The equipment

and accessories described in the manual are manufactured and tested according to industry

standards for safety instrumentation laboratory. Misuse can cause personal injury or damage

to the instrument. Keep this manual at hand close to the instrument, for an easy consultation.

Electrical safety

Before connecting the power cord to wall outlet, ensure that your mains voltage for your

region corresponds to the voltage supply of the instrument, and that the illuminator’s switch

is in position OFF. The user must observe the safety regulations in force in his region. The

instrument is equipped with CE safety marking, in any case the user has full responsibility

concerning the safe use of that instrument.

Warning/Caution symbols used in this manual

The user should be aware of safety aspects when using the instrument. Warning or hazard

symbols are shown below. These symbols are used in this manual.

The instructions on this symbol to avoid possible severe personal injuries.

Warning of use; the incorrect operation on the instrument can cause damages

to the person or instrument.

Possibility of electric shock.

Attention: high temperature surfaces. Avoid direct contact.

Technical notes or usage tips.

Page 4

1.0 DESCRIPTION

Eyepiece

Dioptric adjustment ring

Photo Port selector

lever

Objectives

Iris diaphragm

Field diaphragm

Stage

Nosepiece

Fine focusing knob

Coarse focusing

knob

Tension adjustment

knob

Brighteld brightness adjustment knob (epil-

lumination knob on the other side)

Page 5

1.0 DESCRIPTION

White LED housing

Darkeld lever

Diaphragm centring

screws

Metallographic features

Field diaphragm

Aperture diaphragm

Analyzer lter (rotating)

Polarizer lter Diaphragm centering

screws

Page 6

2.0 INTRODUCTION

This microscope is a scientic precision instrument designed to last for many years with a mini-

mum of maintenance. It is built to high optical and mechanical standards and to withstand daily

classroom and laboratory use.

Optika reminds you that this manual contains important information on safety and maintenance,

and that it must therefore be made accessible to the instrument users.

Optika declines any responsibility deriving from instrument uses that do not comply with this manu-

al.

3.1 The microscope is housed in a moulded Styrofoam container. Remove the tape from the

edge of the container and lift the top half of the container. Take some care to avoid that the

optical items (objectives and eyepieces) fall out and get damaged. Using both hands (one

around the arm and one around the base), lift the microscope from the container and put it

on a stable desk.

3.2 The objectives are located in individual protective vials. Remove the objectives from the vi-

als and insert them into the microscope nosepiece in the order from the lowest magnication

to the highest, in a clockwise direction from the rear.

3.3 In a separate box you nd the metallographic attachment. Take it out and place it onto the

top of the microscope’s stand. Then lock it in position by turning the screw with the provided

allen wrench:

3.0 UNPACKING AND ASSEMBLY

Locking screw for the

attachment

Page 7

3.4 Place the observation head into the top of the attachment and tighten the lock-screw.

3.5 Insert the eyepieces into the eye tubes.

3.6 Insert polarizer and analyzer lters into their slots in the metallographic attachment.

3.7 Connect the power cable of the epillumination to the appropriate connector on the rear.

3.0 UNPACKING AND ASSEMBLY

Connect the mains plug into the socket at the base

Make sure, before you turn the illumination on, that the voltage selector is set to the mains

voltage for your region.

The power cord should be used only on network sockets equipped with adequate grounding.

Contact a technician to check the state of your electrical system. If there is no need to install

additional accessories, the instrument is now ready for use. Once positioned and installed

with the necessary components, the microscope is ready to be used. Your microscope is a

laboratory instrument designed to last. Handle it always carefully and avoid abrupt vibrations

or shocks. Always disconnect the power cable from the microscope when not in use for long

time, while you clean it or when you perform any maintenance.

AVOID DISASSEMBLING THE INSTRUMENT

Do not disassemble the instrument. This entails the cancellation of the warranty and may

cause malfunction.

Page 8

4.1 Adjust the observation head

Loosen the lock-screw, turn the observation head to a comfortable position for observation,

and then lock the lock-screw.

4.2 Place the specimen on the stage

Put the specimen on the mechanical stage (transparent glass plate). Ensure that the speci-

men is centred over the stage opening by adjusting the coaxial knobs of the stage.

Brighteld vision

4.3 Lamp settings

The microscope is tted with a 3.5W X-LED3 illuminator. Insert the plug of the cable into

the power socket and turn on the switch on the back of the main body. Turn the brightness

adjustment knob (on the right of the body) until you hear a “click”, meaning that the lamp is

on, then keep turning the knob to a brightness level suitable for observation.

4.4 Adjust interpupillary distance

Hold the right and left parts of the observation head by both hands and adjust the interpu-

pillary distance by turning the two parts until one circle of light can be seen. After nishing

the interpupillary adjustment, turn the dioptric adjustment ring on the left eye tube to the “0”

position at the graduation line on the eyetube.

4.5 Focus and diopter adjustment

Loosen the focus-stop knob (near the left focus knob), rotate the coarse focusing knob to

bring the slide into focus with an objective with a low magnication, and then lock the focus-

stop knob. Adjust the ne focusing knob to get the image sharp and clear while observing

with your right eye, then turn the left dioptric adjustment ring to a sharp and clear image

also with the other eye. Turn the tension-adjust-knob (near the right focus knob) to get a

suitable tension for the focus system. When the image is in focus, turn the nosepiece to

choose the objective you need.

4.6 Condenser adjustment

Turn the condenser adjustment knob to move the condenser up or down for a clear and

uniform sample illumination. The two condenser-centring screws can be used to centre the

condenser.

4.7 Set the numerical aperture

Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator,

thus controlling image contrast and resolution.

4.8 Field diaphragm

Adjust the eld diaphragm to align the system and to control stray light.

4.9 Additional lters

The chromatic correction lter (blue) can be inserted into the holder (just above the ring of

the eld diaphragm) for denition of specimen parts.

4.0 USING THE MICROSCOPE

Page 9

4.0 USING THE MICROSCOPE

4.10 Video capturing (optional)

B-500MET be connected to cameras via a photo/video adaptor, for photo and video captur-

ing. Before taking a picture or lming video, if necessary, obscure with a dark cloth both the

camera viewnder and the eyepieces and pull out the light path selector lever. Please refer

to the adaptor and camera manuals for further details.

Epillumination vision (metallography)

4.11 Lamp settings

The metallographic attachment is tted with an high power white LED. Insert the plug of the

cable into the power socket and turn on the switch on the back of the main body. Turn the

brightness adjustment knob (on the left of the body) until you hear a “click”, meaning that

the LED is on, then keep turning the knob to a brightness level suitable for observation.

Please note: if no light comes out from the objective, check that both diaphragms on the

metallographic attachment are open (levers UP).

4.12 Adjust interpupillary distance

Hold the right and left parts of the observation head by both hands and adjust the interpu-

pillary distance by turning the two parts until one circle of light can be seen. After nishing

the interpupillary adjustment, turn the dioptric adjustment ring on the left eye tube to the “0”

position at the graduation line on the eyetube.

4.13 Focus and diopter adjustment

Loosen the focus-stop knob (near the left focus knob), rotate the coarse focusing knob to

bring the slide into focus with an objective with a low magnication, and then lock the focus-

stop knob. Adjust the ne focusing knob to get the image sharp and clear while observing

with your right eye, then turn the left dioptric adjustment ring to a sharp and clear image

also with the other eye. Turn the tension-adjust-knob (near the right focus knob) to get a

suitable tension for the focus system. When the image is in focus, turn the nosepiece to

choose the objective you need.

4.14 Field diaphragm

Adjust the eld diaphragm to control stray light. If you want to close the diaphragm, pull

down the lever:

Field diaphragm

lever

Field diaphragm

centering screw

Page 10

At the bottom of the lever, on both sides of the microscopes, there is a couple of screws that

can centre the eld diaphragm.

4.15 Set the numerical aperture

Adjust the aperture of the iris diaphragm to set the numerical aperture of the epilluminator,

thus controlling image contrast and resolution. If you want to close the diaphragm, pull down

the lever:

At the bottom of the lever, on both sides of the microscopes, there is a couple of screws that can

centre the aperture diaphragm.

4.16 Polarization analysis

Slightly pull outwards the polarizer lter (marked “S-PO”) to insert it into the optical path. Turn

the rotating disc of the analyzer lter: if the two lters are correctly inserted, you should nd a

position of the analyzer where no light is transmitted (“crossed polarizers” position).

4.17 Darkeld vision

After you focus the sample with the proper objective, slowly push the darkeld lever toward

the inside. Stop pushing as soon as you see a shadow covering your sample. In this man-

ner you should obtain an oblique illumination that greatly enhances the reliefs and supercial

defects of your sample.

4.0 USING THE MICROSCOPE

Aperture diaphragm

lever

Aperture diaphragm

centering screw

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