Okolab H301-T-UNIT-BL-PLUS Manuel utilisateur
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H301-T-UNIT-BL-PLUS
Electric Top Stage Incubation System
Manual
Vers. 01.17
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Index
INDEX
1PREFACE ............................................................................................................................................................ 5
2SYMBOLS DESCRIPTION .................................................................................................................................... 6
2.1 Symbols in this manual and on the product ...............................................................................6
2.2 Symbols on the product label ................................................................................................6
3SAFETY NOTES .................................................................................................................................................. 7
4SYSTEM OVERVIEW ........................................................................................................................................... 9
4.1 Equipment supplied............................................................................................................9
4.2 Equipment not supplied.......................................................................................................9
4.2.1 Equipment Required........................................................................................................9
4.2.2 Optional ......................................................................................................................9
4.3 Temperature Controller connection ports ............................................................................... 10
5H301-T-UNIT-BL-PLUS. INSTALLATION AND USER GUIDE ......................................................................... 10
5.1 H301-T-UNIT-BL-PLUS. Installation guide ................................................................................ 10
5.2 How to connect H301-T-UNIT-BL-PLUS to a BOLD LINE Digital Gas Controller .................................... 13
6USER INTERFACE............................................................................................................................................. 15
6.1 Home page .................................................................................................................... 15
6.1.1 Colors meaning ............................................................................................................ 15
6.1.2 Changing Set Points....................................................................................................... 16
6.2 Settings ........................................................................................................................ 16
6.2.1 Temperature............................................................................................................... 16
6.2.1.1 Chamber/Adapter............................................................................................................. 16
6.2.1.2 Control Mode ..................................................................................................................... 17
6.2.1.3 Objective Heater .............................................................................................................. 21
6.2.1.4 Thermal Cycles.................................................................................................................. 24
6.2.1.5 Open Incubator ................................................................................................................. 26
6.2.1.6 Ramp Rate.......................................................................................................................... 27
6.2.1.7 External Sensors................................................................................................................ 27
6.2.1.8 Status .................................................................................................................................. 28
6.2.1.9 Digital Input....................................................................................................................... 28
6.2.2 Touch Screen .............................................................................................................. 29
6.2.2.1 Options ............................................................................................................................... 29
6.2.2.2 Brightness........................................................................................................................... 30
6.2.2.3 Calibration ......................................................................................................................... 30
6.2.2.4 Date & Time....................................................................................................................... 30
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6.2.2.5 Alarms................................................................................................................................. 31
6.2.2.6 Visual Effects..................................................................................................................... 31
6.3 Overview Page ................................................................................................................ 32
6.4 Glance Mode .................................................................................................................. 32
7CALIBRATIONS................................................................................................................................................. 34
7.1 Chamber Self-Calibration ................................................................................................... 34
7.2 Objective Heater Manual Calibration ..................................................................................... 35
7.3 Objective Heater Self-Calibration......................................................................................... 37
8MAINTENANCE ................................................................................................................................................. 39
9SUPPORT.......................................................................................................................................................... 40
9.1 QR Codes for Support........................................................................................................ 40
9.2 Support Contacts ............................................................................................................. 40
9.3 Web Conference For Assistance And Training ........................................................................... 40
10 TROUBLESHOOTING.................................................................................................................................. 42
11 TECHNICAL SPECIFICATIONS ................................................................................................................... 43
12 FIGURE LIST ............................................................................................................................................... 44
13 REVISION TABLE ........................................................................................................................................ 45
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1PREFACE
H301-T-UNIT-BL-PLUS is a temperature controller that can operate any Okolab Electrically Heated Stage
Top Incubator to maintain the required thermal conditions for live cell imaging right on the microscope
stage. H301-T-UNIT-BL-PLUS is compatible with Okolab Objective Heaters and Digital Gas Controllers to
maintain the sample in physiological condition with the highest accuracy, thus allowing long term time lapse
imaging.
H301-T-UNIT-BL-PLUS controls the temperature of the incubator base and lid and, when connected, of the
Objective Heater. To improve accuracy on sample temperature, H301-T-UNIT-BL-PLUS is equipped with a
flexible fine gauge thermocouple that can be used to provide feedback from the temperature of a reference
well located in close proximity to the experimental sample. H301-T-UNIT-BL-PLUS is equipped with a room
temperature sensor to monitor laboratory temperature.
H301-T-UNIT-BL-PLUS can be operated via the convenient touch screen interface OKO-TOUCH (not
included), via OKOLAB DataLog Software or via any Third Party Software.
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2SYMBOLS DESCRIPTION
The following symbols identify important information to note:
CAUTION or WARNING: This symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: This symbol warns you of circumstances or practices
that can affect the instrument’s functionality. Please refer to accompanying documents.
Tip -Supplies you with helpful suggestions.
Note -Supplies you with important information to successfully setup and use the instrument.
CE MARKING: This symbol indicates a product’s compliance with EU legislation.
PRODUCT DISPOSAL: This symbol indicates that this product must not be disposed as urban
solid waste.
This symbol indicates the product production date
This symbol indicates the manufacturer data
This symbol indicates the protection degree against ingress of solids or liquids inside the
product.
This symbol warns you to read the user manual before starting the device
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3SAFETY NOTES
Before operating the equipment please read carefully the instructions and the safety notes. If you have any
questions, please contact Okolab.
The equipment must only be used as intended and as described in this Manual.
Equipment should only be operated by technically qualified personnel.
Do not start up the equipment if some of its parts are damaged.
This instrument is not intended for use in locations where flammable or explosive gases are
present.
Handle the equipment with care.
Equipment and its internal parts can be damaged by dropping and by shock.
Do not use a volatile solvent such as paint thinner to clean the instrument. Deformation or
discoloration will occur. Use a soft, dry cloth to remove stains from the instrument. Not
following these instructions can result in damage or breakdown of the device and its
accessories.
The products labels can be found on the bottom panel of the Main Unit.
Do not exceed voltage indicated in this manual and on the product label.
Avoid excessive induction noise, static electricity, magnetic fields.
Do not expose this instrument to rain or moisture.
Prevent throttling and kinking of tubing.
Check the cables are well fixed to their own connectors so they cannot slip off
This device is not designed for use under medical conditions.
Power cord of unit should be unplugged from electrical outlet when left unused for long period
of time.
VENTILATION, unit should be situated so that its' location or position does not interfere with
proper ventilation.
Unit should be situated away from heat sources such as radiators, heat registers,
stoves, or other appliances or processes that produce heat
Do not start up the equipment if the supply cable is damaged.
Connect the equipment only to grounded mains power socket.
Do not disconnect cables while in operation.
Do not open the unit. Do not remove cover or back.
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Prevent metal fragments or lead wire scraps from falling inside instrument case to avoid
electric shock, fire or malfunction.
No user serviceable parts inside.
Before starting, assemble the equipment while unplugged from an outlet.
Unit should never be used where it can fall or be pushed into water.
International caution symbol marks this device. IMPORTANT: read the “Safety Notes”
before installing, using and commissioning this device, as the notes contain important
information relating to safety and EMC. Not following these instructions can result in
damage or breakdown of the device and its accessories.
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES
OF ANY NATURE, LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY
PRODUCT.
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4SYSTEM OVERVIEW
H301-T-UNIT-BL-PLUS includes:
1. T-Unit
2. Fine Gauge Thermocouple. To measure specimen temperature
3. NTC Thermistor. To measure ambient temperature
4. #1 24V-DC Power Adapter
4.2.1 Equipment Required
•Okolab incubating chamber with heated lid and base, chamber riser with one or more plate
adapters
4.2.2 Optional
•OKO-TOUCH, including:
1. Touch Screen Interface
2. #1 5V-DC Power adapter. To connect the Touch Screen.
3. #1 RS232 Serial cable. To connect the Touch Screen to the T-Unit
•Objective heater. Required ONLY when using immersion or dipping objectives.
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Figure 1: Control Unit Rear Panel Overview
1. OKO-TOUCH RS232 serial port.
2. Power Input.
3. Chamber base connector.
4. Lid chamber connector.
5. Objective Heater connector (only if you have ordered also an OBJ-COLLAR-####)
6. USB port.
7. General Purpose Input Output (GPIO) port.
8. NTC Thermistor port.
9. Gauge Thermocouple port.
5H301-T-UNIT-BL-PLUS. INSTALLATION AND USER GUIDE
The following paragraphs illustrate how to install and use the Temperature Control Unit.
The following instructions will guide you through a quick installation. For safe operation of the
unit it is absolutely necessary to read carefully all the instructions and safety notes.
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