Nikon SC Manuel utilisateur

Nikon

MICROSCOPE

MODEL

Instructions

NIPPON

KOGAKU

K.K.

CAUTIONS

HANDLING

CARE

AND

MAINTENANCE

Red-colored

lock

screw

Before

handling

the

microscope,

sure

remove

the

red-colored

lock

screw.

Exchanging

the

lamp

bulb

and

fuse

Before

replacing

the

lamp

bulb

fuse,

sure

turn

the

power

switch

OFF

and

pull

out

the

power

source

cord

from

the

socket.

Place

for

using

Avoid

the

use

microscope

dusty

place,

where

subject

vibrations

exposed

high

temperatures,

moisture

direct

sunlight.

Dirt

the

lens

not

leave

dust,

dirt

finger

marks

the

lens

surfaces.

They

will

prevent

you

from

clear

observa¬

tion

the

specimen

image.

Avoid

sharp

knocks!

Handle

the

microscope

gently,

taking

care

avoid

sharp

knocks.

Use

type

objectives

eyepieces

For

the

microscope,

use

other

objec¬

tives

and

eyepieces

that

type.

Cleaning

the

lenses

clean

the

lens

surfaces,

remove

dust

using

soft

hair

brush

gauze.

Only

for

removing

finger

marks

grease,

should

soft

cotton

cloth,

lens

tissue

gauze

light¬

moistened

with

absolute

alcohol

(eth¬

anol

methanol)

used.

For

cleaning

the

objectives

and

immersion

oil

only

use

xylene.

Observe

sufficient

caution

handling

alcohol

and

xylene.

Cleaning

the

painted

surfaces

Avoid

the

use

any

organic

solvent

(For

example;

thinner,

xylene,

ether,

alcohol

etc.)

for

cleaning

the

painted

surfaces

and

plastic

parts

the

instrument.

Never

attempt

dismantle!

Never

attempt

dismantle

the

instrument

avoid

the

possibility

impairing

the

operational

efficiency

and

accuracy.

When

not

use

When

not

use

cover

the

instrument

with

the

accessory

vinyl

cover

put

the

case

(available

order)

and

store

place

free

from

moisture

and

fungus.

Periodical

checking

maintain

the

performance

the

instru¬

ment,

recommend

the

customers

check

the

instrument

periodically.

For

details,

contact

our

dealers.

CONTENTS

NOMENCLATURE

AND

FUNCTION.

II.

ASSEMBLY.

Attaching

the

eyepiece

tube.

Attaching

the

eyepiece.

Attaching

the

mechanical

stage.

Attaching

the

objectives.

Attaching

the

condenser.

Attaching

the

lamp

bulb.

Attaching

the

lamp

bulb

for

simplified

illumination

III.

OBSERVATION.

Setting

the

specimen

position.

Adjusting

the

illumination.

Adjusting

interpupillary

distance.

Adjusting

for

eyesight

difference.

Coarse

focusing.

Fine

focusing.

Working

distance

objectives.

Vertical

movement

condenser.

Condenser

diaphragm.

10.

Oil-immersion

observation.

11.

Moving

the

glass

ide.

IV.

OPTICAL

FEATURES

MICROSCOPE.H

TROUBLE

SHOOTING

CHART.

Optical

system

Manipulation

system

Electrical

system

ELECTRIC

SPECIFICATIONS.

NOMENCLATURE

AND

FUNCTION

The

descriptions

this

instruction

manual,

for

the

most

part,

cover

the

use

the

binocular

eyepiece

lube

microscope.

They

are

applicable,

except

where

not

specified,

the

monocular

eyepiece

type

well.

Coarse

focus

knob

Power

cord

eyepiece

Diopter

ring

Used

for

eyesight

adjustment.

Binocular

eyepiece

tube

Arm

One

rotation

moves

the

stage

about

24mm.

Fine

focus

knob

Diaphragm

control

ring

Condenser

focus

knob

Microscope

base

Eyepiece

sleeve

Hold

the

sleeves,

when

adjusting

interpupillary

distance.

Revolving

nosepiece

Accepts

objectives-

objective

Range'.

2.2mm

One

rotation

moves

the

stage

about

0.2mm.

Power

switch

and

brightness

control

dial

brightness

obtained

turning

toward

the

thicker

end

the

arrow.

Condenser

Fig.

Slide

holder

Mechanical

stage

Range:

76mm

Filter

holder

For

filters

33mm

diarrwter.

Filter

receptacle

For

filters

45mm

diameter.

Stage

Y-axis

travel

knob

Stage

X-axis

travel

knob

Condenser

clamp

screw

For

locking

the

condenser.

For

tightening,

use

the

tool.

Eyepiece

tube

clamp

screw

Lightly

released,

the

tube

rotatable.

Fig.

tube

Stage

clip

For

holding

the

specimen

position

illuminator

Fig.

II.

ASSEMBLY

Before

handling

the

microscope,

remove

the

red-

colored

lock

screw.

(Fig.

Attaching

the

eyepiece

tube

(Fig.

Slacken

the

eyepiece

tube

clamp

screw

suf¬

ficiently.

Tilting

the

eyepiece

tube

^own

Fig.

seat

horizontal

position.

Fasten

the

clamp

screw

securely.

Attaching

the

eyepiece

(Fig.

Remove

the

dust

caps

from

the

tubes.

)

Screw

the

eyepieces.

avoid

accidental

release,

screw

the

eye¬

piece

firmly,

using

rubber

sheet

the

hold

the

eyepiece.

Attaching

the

mechanical

stage

(Fig.

Place

the

mechanical

stage

the

stage

mount

that

the

travel

knobs

are

the

right

side

operator

facing

the

stage.

Fasten

the

stage

from

the

bottom

with

the

two

4mm

hexagonal

hole

bolts,

using

tool

Attaching

the

objectives

(Fig.

Screw

the

objectives

into

the

revolving

nose-

piece

such

order

that,

the

nosepiece

rotated

clockwise,

objective

higher

magnifying

power

enters

the

optical

path.

For

avoiding

accidental

release,

possible

lock

them

tightly

using

tool

Eyepiece

tube

clamp

screw

Tool

Mechanical

stage

Travel

knobs

Revolvmg

nosepiece

Attaching

the

condenser

Place

the

blue

filter

into

the

filter

holder.

(Fig.

Slide

the

condenser

into

its

mount

far

will

position

where

the

aperture

number

plate

turns

toward

the

operator.

Tighten

the

condenser

clamp

screw,

using

the

tool

(Fig.

10)

Note:

The

tools

and

(Fig.

11)

are

not

included

the

microscope

set.

necessary,

purchase

them

independ¬

ently.

Attaching

the

lamp

bulb

The

microscope

delivered

with

its

lamp

bulb

(100V,

115/125

220/240V-30W)

attached.

necessary

replace

the

bulb

later

time,

proceed

below;

sure

remove

the

power

cord

from

the

socket,

beforehand.

Lay

the

instrument

down

carefully

permit

unscrewing

the

set

screw

found

the

bottom

lid

the

microscope.

screw

driver

coin

may

used.

Sliding

the

bottom

lid

the

side

the

set

screw,

remove

it.

The

lamp

bulb

will

then

accessible.

(Fig.

12)

attach

the

bulb,

pushing

the

lamp

bulb

with

its

silver

surface

turned

the

side

the

bottom

lid

insert

the

bulb

into

the

socket.

(Fig.

13)

Note:

Finger

marks

left

the

lamp

bulb

sur¬

face

will

decrease

the

brightness

image.

handling

the

bulb,

use

cloth

wear

gloves.

Replace

the

bottom

lid.

Attaching

the

lamp

bulb

for

simplified

il¬

lumination

(Fig,

14)

This

lamp

bulb

(110/120V-10W

220/240V

12W)

used

exclusively

for

the

monoc¬

ular

eyepiece

tube

type

microscope.

sure

remove

the

power

cord

from

the

socket.

Remove

the

lamp

socket

from

the

instru¬

ment

turning.

Screw

the

lamp

bulb

into

the

socket

far

will

go.

Reattach

the

socket.

Condenser

clamp

screw

Condenser

Bottom

lid

Aperture

number

plate

Fig.

Tools

available

order

Fig.

Silver

surface

the

lamp

bulb

Fig.

III.

OBSERVATION

Setting

the

specimen

position

Place

the

specimen

the

stage

holding

position

means

the

slide

holder.

{Fig.

15)

When

not

using

the

mechanical

stage,

hold

the

specimen

securely

with

the

two

stage

clips.

(Fig.

16)

Adjusting

the

illumination

Insert

the

power

cord

into

the

socket.

Raise

the

condenser

its

upper

limit,

and

fully

open

the

condenser

diaphragm.

Turn

the

power

switch

brightness

control

dial

light

the

lamp,

(Fig.

17)

Brightness

increased

turning

toward

the

thicker

end

the

arrow,

and

decreased

to¬

ward

the

thinner.

Rotate

the

revolving

nosepiece

swing

the

10X

objective

into

the

optical

path.

this

time,

make

sure

that

the

objective

clicks

properly

into

position,

Adjusting

interpupillary

distance

(Fig.

18)

When

using

binocular

eyepiece

tube,

adjust

the

interpupillary

distance,

that

both

the

right

and

left

viewfields

become

one.

Adjusting

for

eyesight

difference

(Fig.

19)

First,

viewing

into

the

right

eyepiece

with

the

lOX

objective

position,

manipulate

the

coarse

and

fine

focus

knobs

bring

the

specimen

into

focus.

Then,

viewing

into

the

left

eyepiece,

turn

the

diopter

ring

bring

the

image

into

sharp

focus,

without

manipulating

the

focus

knobs.

index

put

the

diopter

ring.

Stage

clips

Fig.

Coarse

focusing

The

coarse

focus

knob

used

for

coarse

focusing

and

for

fine

focusing

with

the

objective.

can

also

used

for

lowering

the

stage

convenience

when

replacing

specimen,

removing

immersion

oil.

etc.

The

relation

between

the

direction

rota¬

tion

the

coarse

focus

knob

and

that

vertical

movement

the

stage

indicat¬

the

arrows

Fig.

20-A.

Fine

Focusing

The

fine

focus

knob

used

for

fine

focusing

with

the

10X

higher

power

objectives.

)

also

used

when

exchanging

objectives

focusing

each

successive

layer

thick

specimen,

The

relation

between

the

direction

rota¬

tion

the

fine

focus

knob

and

that

ver¬

tical

movement

the

stage

the

same

for

the

coarse

focus

knob.

{Fig.

20-A.

The

range

the

fine

focus

knob

2.2mm.

The

white

line

the

fine

focus

knob

shows

the

center

the

fine

focusing

range.

necessary

beforehand

position

the

white

line

the

fine

focus

knob

opposite

the

end

the

coarse

focus

knob.

(Fig.

21)

Working

distance

objectives

"Working

distance"

the

clearance

between

the

front

objective

and

the

upper

surface

the

coverglass

when

the

image

the

specimen

sharp

focus.

this

microscope

follows

for

each

objective:

Objective

Working

distance

4XSC.28mm

lOXSC.8mm

40XSC.0.73mm

lOOXSC.0.16mm

shown

above,

the

working

distances

the

40x

and

100X

objectives

are

very

small,

incurring

the

danger

pressing

the

specimen.

These

two

objectives,

therefore,

are

specifically

equipped

with

safety

de¬

vice,

which

permits

rising

the

front

objective,

the

moment

touches

the

specimen.

Fig.

proceed

focusing,

first

raise

the

stage

the

approximate

working

distance

the

objective

being

used.

Then,

viewing

into

the

microscope,

lower

the

stage

sicwviy,

until

the

image

brought

into

sharp

focus,

Vertical

movement

condenser

For

general

use,

raise

the

condenser

its

upper

limit.

)

When

examining

non-stained

specimens,

preferable

lower

the

condenser

such

extent

that

sufficient

image

contrast

ob¬

tained,

rather

than

the

condenser

diaphragm,

because

has

advantage

that

the

contrast

and

brightness

not

change

abruptly

and

greatly.

Condenser

diaphragm

The

condenser

diaphragm

intended

adjust

its

numerical

aperture

(N.A.)

and

not

the

bright¬

ness

illumination.

When

closed,

the

condenser

diaphragm

will

increase

the

image

contrast

but

decrease

the

resolving

power.

excessively

closed,

causes

fringes

visible

the

edge

image

details.

Opening

the

diaphragm

continuously

adjustable

within

range

from

2.2mm

diameter.

For

general

use,

should

stopped

down

70~80%

the

nu¬

merical

aperture

the

objective

achieve

appropriate

image

contrast.

For

adjust¬

ment

the

diaphragm,

when

observing,

turn

the

diaphragm

control

ring

obtain

image

quality

suitable

for

the

specimen.

10.

il-immersion

observation

(Fig,

21)

When

using

the

100X

objective

marked

with

black

ring,

the

specimen

should

immersed

oil

applying

Nikon

immersion

oil

between

the

coverglass

and

the

front

lens

the

objective.

Fig.

Using

10X

40X

objective

focus

the

specimen.

Bring

the

desired

detail

the

specimen

the

center

the

field

view.

Turning

the

revolving

nosepiece,

place

the

100

objective

position

where

con¬

venient

apply

oil.

Then,

tilt

the

oil

con¬

tainer,

until

oil

enters

the

nozzle.

Depressing

the

container,

attach

appropriate

amount

oil

onto

the

coverglass.

(Fig.

221

The

oil

may

take

air

bubbles.

any

air

bubble

seen,

clear

off

the

oil

using

lens

tissue,

and

apply

the

oil

once

again.

Thereafter,

keeping

the

container

depressed,

apply

the

oil

the

top

end

the

nozzle

that

the

objective.

Then,

rotate

the

100X

objective

into

the

optical

path.

air

bubbles

enter

the

oil

layer,

poor

image

will

result.

remove

the

air

bubbles,

turn

the

revolving

nosepiece

several

times

laterally

apply

additional

drop

oil.

higher

resolving

power

desired,

also

apply

oil

between

the

bottom

the

glass

slide

and

the

condenser

top

lens.

Note:

For

cleaning

the

oil,

after

finishing

oil-

immersion

observation,

wipe

off

thoroughly

with

lens

tissue.

11.

oving

the

glass

slide

The

lateral

travel

range

the

glass

slide

means

the

smaller

knob

the

mechanical

stage

76mm

(2.99

in),

and

the

back

and

forth

travel

range

means

the

larger

knob,

38mm

(1.5

in).

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