LW Scientific M2-P-RH Manuel utilisateur

Head

Binocular (Seidentopf)

Optional trinocular with C-mount or eye tube

35mm and digital camera adapters available

Diopter adjustment

Inclined 30° (45° available), rotates 360°

10X/20 high point eyepieces (10X/22 available)

Interpupillary distance range 50-75mm

Nosepiece

Reverse quadruple nosepiece

Multiple ball bearing mounted

Objectives

DIN Plan flat-field objectives

4X, 10X, 40XR, 100XR (oil)

Anti-fungal, parfocal, parcentric, color-coded

Stage

Mechanical stage (160mm x 140mm)

Coaxial drive controls, metal rack & pinion

Range of traverse: 77mm x 50mm

Stage locking lever

Slow-close hydraulic slide finger

Dual slide holder

Focus

Coarse adjustment: range of 20mm

Fine adjustment: graduation of 2µm

Tension control knob

Illumination

Polarizing kit included - see back for instructions

Moveable Abbe condenser, NA 1.25

Iris diaphragm

Halogen light source (12V/20W bulb)

Variable light adjustment: 0-20W output

Input 90-240V / 50-60Hz automatic switching

Accessories Included

One 2 amp fuse, spare 12V/20W bulb, blue, green,

and yellow filters, dust cover, immersion oil, manual

and warranty card

Options

Ceramic stage, dark field condenser, phase contrast

turret, and Koehler-type illumination

Warranty

Lifetime on materials and workmanship

One year on electronic components

Dimensions and Weight

Height: 16 1/2” (420mm)

Length: 10 5/8” (270mm)

Width: 7 7/8” (200mm)

Weight: 19.5 lbs. (9kg)

Laboratory Microscopes

The M2 Rheumatology LabScope is an essential tool

for synovial fluid analysis. Crystal-induced arthritis,

such as gout or pseudogout, can be identified and

differentiated with the use of the polarizing attachment

and red compensator lens. The Rheumatology

LabScope also functions as a flat-field, laboratory-

grade microscope for routine cytology, hematology,

urinalysis, etc. This specialized system was

designed to combine high-resolution optics with

functionality at an affordable price for the

Rheumatologist. The M2 LabScope provides comfort,

durability, dependability and superior imaging for the

lifetime of the laboratory, and the lifetime warranty

proves it.

Recommended upgrade:

Koehler Illumination

On the web: www.lwscientific.com - email: [email protected]

865 Marathon Parkway - Lawrenceville, Georgia 30045 - Ph: 770-270-1394 - Fx: 770-270-2389

Rheumatology LabScope

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Rheumatology Polarization

Installing and Operating Instructions

Introduction:

Polarized light microscopy is used for observation of crystalline and mineral specimens, because of their optically

anisotropic character. Contrast occurs due to polarized light interacting with the birefringence (double refraction) of

crystals. The polarizer is a two-stage filter assembly (polarizing lens and red compensator lens) that must be

positioned in the light path below the specimen, and the analyzer (smaller polarizing lens) must be positioned above

the specimen and objectives and beneath the head.

When the two filters are aligned parallel with each other, the polarized light path is transmitted through both filters and

the field will be at its brightest, but when the polarizer (lower) is turned 90 degrees the light is extinguished. At this

cross-polarized setting anisotropic crystals will appear to glow against a relative dark background, and when the red

compensator lens is used the birefringent material will show as either blue or yellow against a red background

depending on the material’s orientation.

Polarization is an investigative tool used to distinguish different types of arthritis. It has many other applica

tions as well.

Features:

The polarization kit is only available on M-Series LabScopes. It includes two parts which are easily installed in less

than 1 minute. These quality filters combined with the bright illumination and PLAN optics of the M-Series LabScopes

produce clear contrast and high resolution images.

Installation and Setup:

Remove the head of your M-Series LabScope.

Install the analyzer (the smaller filter...about the diameter of a quarter) by placing it into the small opening

inside the head mounting circle on the microscope. Make sure the small screw on the side of the filter fits

into the slot inside the opening. You may leave the analyzer in place for brightfield use if you choose.

Re-install your head.

Remove the red compensator lens (clear lens with plastic handle) from the lower polarizer assembly by

simply pulling it out of the housing. Set it aside.

Install the polarizer by loosening the thumb screw and placing it over the base illuminator lens. Turn on

the microscope, select the 10x or 40x objective, and look through the eyepieces to view the

light field. Turn the polarizer 90 degrees either direction to achieve the darkest light setting

and then tighten the screw. Make sure that the slot for the red compensator lens is not

facing to the rear.

Using a sample specimen slide, focus on an active crystal which should be glowing and then slide the red

compensator lens into place over the polarizer.

Rotate the red compensator lens to achieve a red background. Crystals will show either positive or

negative birefringence, by turning either yellow or blue in relation to their orientation. By rotating the red

compensator lens 90 degrees, you will notice the color of the crystals change from blue to yellow, or

yellow to blue.

Use positive test slides to create a protocol for diagnosing gouty or pseudo gouty arthritis. Crystals

should be identified by both their morphology and their direction of birefringence.

When finished, remove the polarizer and store carefully in a dust free container.

Care and maintenance:

Clean the filters very delicately with lens paper and lens cleaning solution only if dirty.

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