Labnet Spectrafuge 6C Manuel utilisateur

Lit M00031 Rev 2 August 2016

SPECTRAFUGE™ 6C

User Manual

C0060

C0060-230V

About This Manual

This manual is designed to assist you in the optimal usage of your Labnet Spectrafuge

6C Centrifuge. The manual is available in English, French, German, Italian,

Portuguese, and Spanish on our website at:

www.labnetinternational.com\document-center

Safety Precautions

NEVER use the centrifuge in any manner not specified in these instructions.

NEVER operate the centrifuge without a rotor properly attached to the shaft.

NEVER fill tubes while they are in the rotor. Liquid spillage may harm unit.

NEVER put hands in the rotor area unless the rotor is completely stopped.

NEVER move the centrifuge while the rotor is spinning.

NEVER use solvents or flammables near this or other electrical equipment.

NEVER centrifuge flammable, explosive or corrosive materials

NEVER centrifuge hazardous materials outside of a hood or proper containment

facility

ALWAYS load the rotor symmetrically. Each tube should be counterbalanced by

another tube of the same type and weight

ALWAYS locate the centrifuge within easy access to an electrical outlet.

ALWAYS use only centrifuge tubes designed to withstand centrifugal forces of at

least 4,000 xg.

ALWAYS use a wrench to tighten rotor nut.

Symbols and Conventions

The following chart is an illustrated glossary of the symbols that may be used in this manual or

on the product.

The electrical warning indicates the presence of a potential hazard which could

result in electrical shock.

CAUTION This symbol refers you to important operating and maintenance

(servicing) instructions within the product Instruction Manual. Failure to heed this

information may present a risk of damage or injury to persons or equipment.

Reproduction without prior written permission is prohibited.

Information contained herein is subject to change without notice.

Table of Contents

1. General Information 1

Description

Safety precautions

Technical data

Accessories supplied with unit

Warranty

2. Installation 2

Unpacking the centrifuge

Required space

Installing the centrifuge

3. Installing the Rotor 2

Rotors and accessories

Rotor maintenance

Removing and installing the angle rotor

Overloading rotors

4. Operation 5

Closing the lid

Lid release

Lid lock

Speed selection

Selection of operating time and momentary operation

Starting the Centrifuge

5. Service and Maintenance 6

Service

Cleaning

Disinfection

Replacing fuses

6. Troubleshooting 7

7. Where to Call 8

8. Determination of g-values 8

1. General Information

This manual provides important safety information for the Spectrafuge 6C

laboratory centrifuge. It should be kept near the centrifuge for quick and easy

reference.

1.1 Description

The Spectrafuge 6C is a small benchtop centrifuge designed for separation

of various research samples. The Spectrafuge 6C is supplied with a 6 x

15ml rotor. Adapters are available for tubes smaller than 10ml. The

Spectrafuge 6C reaches speeds of up to 6,500rpm/4,000 x g.

1.2 Safety precautions

Note: All users of the centrifuge must read the Safety Precautions

section of this manual before attempting to operate the unit!

If this equipment is used in a manner not specified by the

manufacturer, the protection provided by the equipment may be

impaired.

Do not operate the centrifuge if any of the following conditions exist:

-The centrifuge has not been installed properly

-The centrifuge is partially dismantled

-Service has been attempted by unauthorized or unqualified personnel

-The rotor has not been installed securely on the motor shaft

-Rotors and accessories not belonging to the standard range are being used

without permission being obtained from the manufacturer to use such

rotors and/or accessories in the centrifuge

Exception: Centrifuge tubes, normally available in the laboratory.

-The centrifuge is located in an explosive atmosphere

-Materials to be centrifuged are combustible and/or explosive

-Materials to be centrifuged are chemically reactive

-The rotor load is not properly balanced

1.3 Technical data

Dimensions

Width 8.25 inches

Depth 9.5 inches

Height 7.0 inches

Maximum speed 6,500rpm

Maximum RCF 4,000 x g

Maximum volume 6 x 15ml

Admiss. density 1.2kg/dm3

Electrical/fuse rating 100V~,50-60Hz, 1.0A/1.0 AT

120V~, 50-60Hz, 1.0A/1.0AT

230V~, 50-60Hz, 0.6A/.05AT

1.4 Accessories supplied with centrifuge

Each unit is supplied with 1 instruction manual and 1 power cord. Some

models are supplied with a rotor screw wrench.

1.5 Warranty

This centrifuge has been subject to thorough testing and quality control. In

the unlikely event of a manufacturing fault, our one year warranty (from the

date of delivery) covers the centrifuge and the rotor. This warranty

becomes invalid in the case of incorrect operation, use of nonstandard

spare parts or accessories and unauthorized modification of the rotor or

centrifuge.

Labnet reserves the right to make technical modifications. Statements

contained herein are not to be considered binding.

2. Installation

2.1 Unpacking the centrifuge

Before unpacking the centrifuge, inspect the outside of the carton for any

shipping damage.

The centrifuge is delivered in a carton with protective cushions. Remove

the centrifuge from the carton. Retain the carton and cushions until it has

been established that the centrifuge is working properly.

Inspect the centrifuge for any visible signs of shipping damage.

Shipping damage is the responsibility of the transportation carrier. Any

claims for damage must be filed within 48 hours.

The accessories supplied with the centrifuge should be kept with the

instruction manual near the centrifuge’s place of installation.

2.2 Required space

The centrifuge should be installed on a rigid, even surface such as a stable

laboratory bench, cabinet, etc. To guarantee sufficient ventilation, ensure

that the centrifuge has at least 15cm (6 inches) of free space on all sides,

including the rear.

The centrifuge should not be located in areas subject to excessive heat

such as in direct sunlight or near radiators or the exhaust of a compressor,

as a buildup of heat may occur within the chamber.

2.3 Installation

Before operating the centrifuge, check that the power source corresponds to

that on the manufacturer’s rating label, then connect the power cord to the

centrifuge and the power source.

3. Installation or rotors and rotor maintenance

3.1 Rotors and accessories

The following accessories are available for the Spectrafuge:

Angle rotor for 6 x 15ml tubes

Order no. Included with unit (C0060-RTR)

Tube measurement 15ml (17x120mm)-10ml (16x100mm)

Max. speed 6,500rpm

Centrifuging radius 8.5cm

RCF (g-value) 4,000 x g

Adapter for 5ml(12x75mm) and 7ml (13x100mm) tubes

Order no. C0200-17A

Tube measurement 12x75mm, 13x100mm, or common

Sarstetd style tubes

Tube and tube adapter reference chart.

3.2 Rotor maintenance

The rotor should be cleaned thoroughly after each use. Thorough

cleaning must be performed when spinning samples containing

phenol or phenol chloroform. Periodically inspect the rotor for dents,

dings, scratches, discoloration and cracks If any damage to the rotor is

found, discontinue use of the rotor immediately and replace.

3.3 Removing and Installing the angle rotor

Remove the rotor screw from the motor shaft by turning the screw

counterclockwise. Lift the rotor upward and remove from the centrifuge.

Ensure that the motor shaft adapter remains on the motor shaft (Figure 1).

Clean the motor shaft and motor shaft adapter (see figure 1).

Place the rotor on the motor shaft (figure 1) and over the motor shaft

adapter (see figure 1 and 2). Note: Figure 1 and 2 are located on the

following page.

When loading the rotor, refer to figure 3 (located on the following page). Loading

in the pattern indicated will ensure a balanced load. Tubes to be loaded should be

filled equally by eye. The difference in the weight between the tubes should not

exceed 2-3 grams. A partially loaded rotor may be centrifuged if the loading

scheme for balancing a rotor given in figure 3 is followed.

Figure 1. Chamber and motor shaft

Figure 2. Bottom of angle rotor

Figure 3. Loading the rotor

3.5 Overloading the rotor

The maximum load of the rotor and the maximum speed has been

established by the manufacturer. Do not attempt to exceed these values.

The maximum speed of the rotor has been measured for liquids having a

homogeneous density of 1.2g/ml or less. In order to centrifuge liquids with a

higher density it is necessary to reduce the speed.

Failure to reduce the speed may result in damage to the rotor and

centrifuge.

The revised maximum speed can be calculated with the following formula:

Reduced speed (nred) = x max speed (nmax)

Example:

Where the density of the liquid is 1.7, the new maximum speed would be

calculated as follows:

nred = x 6,500 = 5,461 rpm

If in doubt concerning maximum speeds, please contact the manufacturer

for assistance.

4. Operation ATTENTION: Never attempt to operate the centrifuge with rotors or

adapters that show signs of corrosion or mechanical damage. Never

centrifuge strongly corrosive materials that may damage the rotors or

accessories.

4.1 Closing the lid

After the rotor has been properly secured and loaded, close the centrifuge

lid, making sure that the interlock has been engaged.

Figure 4.

Spectrafuge

control panel

layout

4.2 Lid release

Following a run, the centrifuge will display will show flashing “00”. This

signals the end of a run and the lid can now be opened by pressing the “lid

knob” (left). Note that the lid can not be opened until the display flashes

“00” and the rotor has stopped.

WARNING: Do not attempt to open the lid of any centrifuge

until the rotor has come to a complete stop.

In the event of a power failure or malfunction, it may be

necessary to open the lid manually.

1. Disconnect the power cord from the wall socket.

2. Remove the plastic plug, located on the left side of the unit, below the

quick button.

3. Pull the cord (attached to the plug) to open the lid lock manually.

4.3 Lid lock

The centrifuge can be started only with the lid securely closed. Do not

attempt to open the lid until the end of run signal “00” is displayed.

4.4 Speed selection

The speed (rpm) can be selected from 300 to 6,500rpm with the knob (right).

The set speed can be viewed at all times on the large LED display (right).

4.5 Selection of operating time and momentary operation

Time can be selected in half minute intervals from 0.5 to 10 minutes, and in

one minute intervals from 11 to 30 minutes. Time can also be set to

continuous/hold by turning the timer knob past the thirty minute position.

This will display the continuous setting “On”.

When the preselected time expires, the centrifuge will stop automatically.

To stop the centrifuge prior to the expiration of set time, press the

“start/stop” knob.

The centrifuge may be operated manually by pressing and holding the

“start/stop” knob. The centrifuge will continue to run as long as the knob is

depressed.

4.6 Starting the centrifuge

Once the time and speed have been set the centrifuge can be started by

pressing the “start/stop” knob. The centrifuge will then run for the specified

amount of time.

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