Hitachi TM4000 Fiche technique
(1) Turn on the earth leakage breaker on the back of the main unit.
(2) Turn on the power switch on the right side of the main unit to start the
device.
(3) When the unit is turned on the Evacuation process will start
automatically and the EVAC LED will blink BLUE.
If you want to load a sample, press the EVAC/AIR button. The AIR LED will
then blink WHITE. Once the specimen chamber is at air, the AIR LED will be
stop blinking and remain WHITE.
(4) Turn on the PC. Start the TM4000/TM4000Plus application by double-
clicking the TM4000 programme shortcut. The TM4000 start screen will be
displayed until the Graphical User Interface (GUI) is loaded.
Bulk specimen (Conductive/Non-conductive) Water / Oil containing specimen
(5) Put the conductive tape on the specimen stub and attach
a specimen on it. (5) When observing water containing specimens such as
biological and botanical specimens, food items and so on,
use a paste to stick a specimen on the stub.
(6) Attach the specimen stub to the specimen holder, and adjust its height using a height gauge so that the
gap width becomes about 1 mm.
TM4000/TM4000Plus
Simple Manual
Front of TM4000
1. Starting The Instrument
2. Preparing Specimen
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(7) Ensure the chamber is at air, ie AIR LED is not
blinking and is WHITE.
If the EVAC LED is Blue, press the EVAC/AIR switch. The
AIR LED (WHITE) should start blinking. When the AIR
LED stops blinking and remains illuminated the
chamber is at AIR.
3. Loading the Specimen
(8) After the AIR LED stops blinking wait approximately
five seconds or more before slowly drawing out the
specimen stage.
(9) Insert specimen stub assembly into the stage base.
(10) OPTIONAL Lightly tighten the hex screw to secure
the specimen stub assembly to the stage base.
(11) Use the X, Y knobs to centre the specimen stage
by lining up the centre position mark with the guides.
(12) Press the EVAC/AIR Switch to evacuate the
chamber. The EVAC LED will blink BLUE while the
chamber is being evacuated. Once evacuation is
complete (~2.5 min) the EVAC LED will stop blinking
and remain illuminated (BLUE)
(13) Select Accelerating Voltage / Observation condition.
4. Starting Observation
(14) Select Vacuum level
(15) Select Detector. Can change selection later so not
critical to select at this point.
(16) Click the button to turn on the high voltage.
(17) Use the auto functions
a) Set desired field of view using the X, Y knobs on the
front of the TM4000.
b) Adjust the Brightness and Contrast (click the Auto
B/C button.
c) Perform focusing (click the Auto Focus button)
a) Perform manual focus adjustment if
required.
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(18) Click the “Save” button to capture the image
5. Saving Captured Images
(20) Click the button to turn off the high voltage.
6. Stopping Observation
(21) To remove the sample press the EVAC/AIR switch to
introduce air into the specimen chamber. Wait until the AIR
LED (WHITE) stops blinking and remains illuminated.
(22) Slowly draw out the specimen stage, undo the hex
mounting screw and remove the specimen stub assembly
from the stage.
(23) Close the specimen stage door.
(24) If you are not looking at any other specimens, press the
EVAC/AIR switch to evacuate the chamber. The EVAC LED
(BLUE) will blink while the chamber is evacuated and will stop
blinking and remain illuminated once the chamber is under
vacuum (~2.5 min)
(19) After the image capturing process is completed
the Save Image dialog will be displayed. Input a file
name and save the image.
NOTE: Quick Save will save the image on the screen, ie
lower resolution than if you use the Save button.
(25) Make sure the chamber is under vacuum, ie EVAC LED
is not blinking and remains illuminated (BLUE)
7. Shutting Down The Instrument
(26) Close the TM4000 Graphical User Interface (GUI)
(27) Turn off the Power Switch on the right side of the
TM4000.
NOTE: Always leave the chamber under vacuum when
system not in use. This helps keep dust and moisture out of
chamber to ensure the microscope remains in good
condition.
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1
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