Harvard Bioscience BTX 630B Manuel utilisateur

Safety Stands

USER’S MANUAL

Publicaon 5502-002 Rev 2.0

45-0207 (Model 630B) Cuvee Safety Stand

47-0208 Extended Capacity Flatpack Safety Stand

47-0203 AgilePulse MAX Cuvee Safety Stand

47-0209 AgilePulse MAX Extended Capacity Flatpack Safety Stand

47-0610N AgilePulse PLUS Extended Capacity Flatpack Safety Stand

Thank you for invesng in a Safety Stand.

Since its founding in 1983, the main focus of BTX has been in the area of applying

controlled electric elds for genec engineering applicaons. Because of this,

we quickly established a reputaon as the technological leader in the elds of

electroporaon and electrofusion. Our systems have been installed in many

presgious instutes around the globe where they are used successfully for high

eciency transfecon, transformaon and cell fusion applicaons. We oer a

variety of waveforms, electrodes and chamber opons to provide you with the

best tools to achieve your goals.

We are vested in your success. To that end, the BTX technical support team

constantly tracks published literature for any reference to electroporaon and

electrofusion. We extract the pernent experimental condions and yields

from these papers to help us in our eorts to help you. In addion to tracking

publicaons, we are available to you for support at any me for advice in

experimental design, product recommendaons, troubleshoong, and any other

relevant technical advice.

We thank you again for your investment and we look forward to assisng you in

any way we can.

Finally, please read this manual carefully before operang. If you have any

quesons about the unit or about parcular applicaons, please contact us:

BTX

84 October Hill Road

Holliston, MA 01746 USA

Toll Free: 1-800-272-2775

Internaonal Callers: 508-893-8999

Fax: 508-429-5732

Web: www.btxonline.com

Email: [email protected]

For any customers outside the US or Canada, please call your local BTX dealer

or call us directly.

A Message from BTX

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

Table of Contents

SUBJECT PAGE #

Warranty Informaon 2

General Safety Informaon 3

Introducon 4

Operaon: Geng Started 5

Specicaons 6

Ordering Informaon 7

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

Warranty Information

FOR RESEARCH USE ONLY

BTX

84 October Hill Rd

Holliston, MA 01746, USA

Phone: 1-508-893-8999

Fax: 1-800-429-5732

Web: www.btxonline.com

Warranty

BTX warranes this Safety Stand for a period of 90 days from

the date of purchase. At its opon, BTX will repair or replace the

unit if it is found to be defecve as to workmanship or materials.

This warranty does not extend to equipment which has been (a)

subjected to misuse, neglect, accident or abuse, (b) repaired or

altered by anyone other than BTX without

BTX express and prior approval, (c) used in violaon of instrucons

furnished by BTX. This warranty extends only to the original

customer purchaser. IN NO EVENT SHALL BTX BE LIABLE FOR

INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some states do not

allow exclusion or limitaon of incidental or consequenal damages

so the above limitaon or exclusion may not apply to you. THERE

ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS

FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states

do not allow this limitaon on an implied warranty, so the above

limitaon may not apply to you. Without liming the generality

of the foregoing, BTX shall not be liable for any claims of any kind

whatsoever, as to the equipment delivered or for non-delivery of

equipment, and whether or not based on negligence. Warranty is

void if the instrument is changed in any way from its original factory

design or if repairs are aempted without wrien authorizaon

by BTX. Warranty is void if parts, connecons, or power supplies

not manufactured by BTX are used with the BTX Safety Stand. If a

defect arises within the warranty period, promptly contact BTX, 84

October Hill Road, Holliston, Massachuses, USA 01746-1388 using

our toll free number 1-800-272-2775 (US Only) or 508-893-8999.

(E-mail: [email protected]). Goods will not be accepted for

return unless an RMA (Returned Materials Authorizaon) number

has been issued by our customer service department. The customer

is responsible for shipping charges. Please allow a reasonable

period of me for compleon of repairs, replacement and return.

If the unit is replaced, the replacement unit is covered only for the

remainder of the original warranty period dang from the purchase

of the original device. This warranty gives you specic rights, and

you may also have other rights, which vary from state to state.

Out of Warranty Service

Proceed exactly as for Warranty Service above. If our service

department can assist you by phone or other correspondence,

we will be glad to help at no charge. Repair service will be billed

on the basis of labor and materials. A complete statement of

me spent and materials used will be supplied. Shipment to BTX

should be prepaid. Your bill will include return shipment freight

charges. Disassembly by the user is prohibited. Service should

only be carried out by experienced BTX technicians.

Repair Facilies and Parts

BTX stocks replacement and repair parts. When ordering, please

describe parts as completely as possible, preferably using our part

numbers. If praccal, enclose a sample photo or drawing.

Cauon Noce

The BTX Safety Stand is intended for laboratory use only and can

be used in research and development applicaons. These systems

have been designed to meet the standards for electromagnec

compability (EMC) and safety. This product should not be used

in the presence of a ammable atmosphere such as an anesthec

mixture with air, oxygen, or nitrous oxide.

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

Safety Information

Please read the following safety precauons to ensure proper use of

your Safety Stand. If the equipment is used in a manner not specied,

the protecon provided by the equipment may be impaired.

To Prevent Hazard or Injury

Make Proper Connecons

Make sure all connecons are made properly and securely.

Any signal wire connecons to the unit must be no longer than

three meters.

Observe All Terminal Rangs

To avoid re or shock hazard, observe all rangs and markings on

the product or in this manual before using the device.

Avoid Exposed Circuitry

Do not touch any electronic circuitry inside of the product. Do not

insert ngers or try to remove electrode or sample during pulsing

sequence.

Do Not Operate with Suspected Failures

If damage is suspected on or to the product do not operate the

product. Contact qualied service personnel to perform inspecon.

Place Product in Proper Environment

Review the operang manual for guidelines for proper operang

environments. Do not operate in wet/damp condions.

Wear proper eye protecon

during electroporaon

Arcing Can Occur at High Voltages

An unfavorable combinaon of parameters such as high voltage

sengs and a small sample volume with a highly conducve

medium might lead to ashover between the electrodes (ARC) and/

or explosive evaporaon of the medium. Reduce voltage or pulse

length to avoid repeang this condion.

Observe all Warning Labels on Product

Read all labels on product to ensure proper usage.

High Voltage Risk

These BTX Safety Stands are designed to connect to a high voltage

power supply adjustable to 3,000 V. High voltage power supplies

present a serious risk of personal injury if not used in accordance

with design and/or use specicaons, if used in applicaons on

products for which they are not intended or designed, or if they

are used by untrained or unqualied personnel.

• The user must read this manual carefully before the

instruments are placed into operaon.

• Removing the cover will void the warranty.

• Do not connect or disconnect the high voltage cable with

the high voltage enabled.

• To connect or disconnect the cable, turn line power o and

unplug line (mains) cord.

• Do not touch the electrode p while the waveforms are

being applied.

If there are any quesons about the operaon of this equipment,

call BTX Customer service at 1-800-272-2775, or 1-508-893-8999.

Cauon Noce

The BTX Safety Stand is intended for laboratory use only and can

be used in research and development applicaons. These systems

have been designed to meet the standards for electromagnec

compability (EMC) intended for laboratory equipment

applicaons as well as the applicable safety requirements for

electrical equipment for measurement, control, and laboratory

use. The unit itself does not generate waste, but may be used to

treat samples that are hazardous. Please use appropriate PPE and

ensure disposal in accordance with local regulaons and pracces.

This product should not be used in the presence of a ammable

atmosphere such as an anesthec mixture with air, oxygen, or

nitrous oxide.

Cauon Risk

of Electric Shock

Cauon

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

The BTX Safety Stand is a safety device for use with BTX disposable cuvees and atpack chambers. Its unique design ensures that no

current can be delivered unless the lid is closed, therefore protecng the user from high voltages. Up to two cuvees can be electroporated

at once, thus decreasing experimentaon mes. Please refer to the Product Compability table below for compable BTX electroporators

and accessories.

Introduction

Product Compability

BTX Safety Stand Item # 45-0207 47-0208 47-0203 47-0209 47-0610N

Compable

BTX Generators

ECM 399

ECM 630

ECM 830

ECM 2001*

Gemini X2

* When used with

F/F adapters,

Item 45-0088,

Sold separately

ECM 399

ECM 630

ECM 830

ECM 2001**

Gemini X2

** When used

with included

F/F adapters,

Item 45-0088

AgilePulse MAX

ECM 2001+

AgilePulse MAX

ECM 2001+

AgilePulse PLUS

Compable

BTX Cuvees

All 1 mm, 2 mm,

and 4 mm gap

BTX cuvees

All 1 mm, 2 mm,

and 4 mm gap

BTX cuvees

All 1 mm, 2 mm,

and 4 mm gap

BTX cuvees

All 1 mm, 2 mm,

and 4 mm gap

BTX cuvees

All 1 mm, 2 mm,

and 4 mm gap

BTX cuvees

Compable

BTX Flatpacks

45-0109

45-0110

45-0109

45-0110

47-0206

45-0109

45-0110

45-0109

45-0110

47-0206

45-0109

45-0110

47-0206

Note: For compability with older models, please contact BTX Technical Support.

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

Warning High Voltage

Make sure the BTX electroporator

is switched o before connuing.

1. Aach the safety stand cables to the output on the rear or

the front panel of the electroporator depending on the BTX

electroporator being used. For AgilePulse MAX, AgilePulse PLUS,

and ECM 2001+, also connect interlock cable to front panel of

electroporator. For ECM 399, ECM 630, ECM 830, and Gemini

electroporators, connect safety stand cables directly to generator

high voltage output ports. For Legacy ECM 2001 connect safety

stand cables to HV output cables using F/F adapters.

2. Using asepc technique, pipee the correct volume of cell

suspension and reagents into the cuvee or atpack chamber.

If using a cuvee or 10 ml atpack chamber (Item 47-0206),

replace the cap.

3. Open the safety stand shield and place the cuvee or atpack

chamber between the metal contacts. Adjust the distance

between the contacts using the black roller. (Note: the

adjustment will remain the same aer the rst me, do not

readjust the roller with every cuvee. However, check contact

periodically to ensure good current ow). Close the safety

stand shield.

4. Switch on the BTX electroporator. Check that all instrument

sengs and connecons are correct. Deliver electroporaon

pulse protocol by pressing the appropriate START or PULSE

buon depending on the BTX electroporator being used.

5. Aer the pulse delivery is complete, remove the cuvee or

atpack chamber from the safety stand. Use a pipee to remove

the pulsed cells. Treat the pulsed cells according to the protocol.

6. Discard the cuvee, pipee and/or atpack chamber in

a biohazard container. Refer to your local instuon’s

environmental health and safety regulaons for proper

disposal pracces.

7. Unplug safety stand from electroporator. Clean plasc lid with

ethanol and deionized water as needed. Please contact Technical

Support for guidance regarding cleaning procedure for safety

stand metal electrode contacts.

Operation: Getting Started

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

Standard Capabilies:

Voltage Range 0 to 3000 VDC; 0 to 250 VAC

Frequency 0.2 to 2.0 MHz

Pulse Length/ 10 μs to 10 s

Time Constants Range

Other Specicaons:

Storage Temperature -10 to 70°C

Operang Temperature 5 to 40°C

Intended Use Indoor Use Only

Relave Humidity 20 to 80%

Maximum Altude 2,000 m (6,562 )

Polluon Degree II

Insulaon Category CAT I

Physical Characteriscs:

Overall Dimensions

(45-0207, 47-0203) L x W x D

12.7 x 10.2 x 17.8 cm (5 x 4 x 7 in)

Overall Dimensions

(47-0208, 47-0209) L x W x D

12.7 x 10.2 x 19.0 cm (5 x 4 x 7.5 in)

Weight

1 kg (2.3 lb)

Speciﬁcations

Safety Stand

5502-002 Rev 2.0 • www.btxonline.com

Ordering Information

Safety Stands

45-0207 Cuvee Safety Stand (Model 630B) 1 Ea

47-0208 Extended Capacity Flatpack Safety Stand 1 Ea

47-0203 AgilePulse MAX Cuvee Safety Stand 1 Ea

47-0209 AgilePulse MAX Extended Capacity Flatpack Safety Stand 1 Ea

47-0610N AgilePulse PLUS Extended Capacity Flatpack Safety Stand 1 Ea

Replacement Parts

45-0213 Replacement Acrylic Safety Stand Cover (for 45-0207 and 47-0203) 1 Ea

45-0215 Metal Replacement Kit (for 45-0207 and 47-0203) 1 Kit

Accessories and Adapters

45-0208 Cuvee Rack (Model 660) 1 Ea

45-0088 F/F adapters, red and black, for 4 mm diameter banana cables and pin ps 1 Kit

45-0217 Banana to banana cables, red and black, 10  length 1 Kit

47-0097 Adapter, AgilePulse PLUS Generator to AgilePulse MAX Stands 1 Ea

Consumables

45-0134 BTX Cuvees Plus, 1 mm gap, 90 µl, Sterile, Gray cap, individually wrapped, with transfer pipees Pkg. of 10

45-0124 BTX Cuvees Plus, 1 mm gap, 90 µl, Sterile, Gray cap, individually wrapped, with transfer pipees Pkg. of 50

45-0140 BTX Cuvees Plus, 1 mm gap, 90 µl, Sterile, Gray cap, bulk wrapped in packages of 100 cuvees per pkg. Pkg. of 100 x 24

45-0135 BTX Cuvees Plus, 2 mm gap, 400 µl, Sterile, Blue cap, individually wrapped, with transfer pipees Pkg. of 10

45-0125 BTX Cuvees Plus, 2 mm gap, 400 µl, Sterile, Blue cap, individually wrapped, with transfer pipees Pkg. of 50

45-0141 BTX Cuvees Plus, 2 mm gap, 90 µl, Sterile, Blue cap, bulk wrapped in packages of 100 cuvees per pkg. Pkg. of 100 x 24

45-0135 BTX Cuvees Plus, 4 mm gap, 800 µl, Sterile, Yellow cap, individually wrapped, with transfer pipees Pkg. of 10

45-0125 BTX Cuvees Plus, 4 mm gap, 800 µl, Sterile, Yellow cap, individually wrapped, with transfer pipees Pkg. of 50

45-0141 BTX Cuvees Plus, 2 mm gap, 800 µl, Sterile, Yellow cap, bulk wrapped in packages of 100 cuvees per pkg. Pkg. of 100 x 24

45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume Pkg. of 50

45-0110 Flatpack Chambers, 0.56 mm gap, 80 ml volume Pkg. of 50

47-0206 Flatpack Chambers, 4 mm gap, 10 ml volume Pkg. of 10

47-0002 BTXpress Cytoporaon Medium T Low Conducvity Electroporaon Medium, 500 ml bole 1 Ea

45-0801 BTXpress Electroporaon Soluon, 2 ml bole, up to 20 reacons 1 Ea

45-0802 BTXpress Electroporaon Soluon, 5 ml bole, up to 50 reacons 1 Ea

45-0805 BTXpress Electroporaon Soluon, 10 ml bole, up to 100 reacons 1 Ea

45-0803 BTXpress Electroporaon Soluon Kit, 50 reacons in 2 mm gap cuvees 1 Kit

45-0804 BTXpress Electroporaon Soluon Kit, 20 reacons in 4 mm gap cuvees 1 Kit

45-0806 BTXpress Electroporaon Soluon Kit, 100 reacons in 2 mm gap cuvees 1 Kit

45-0807 BTXpress Electroporaon Soluon Kit, 40 reacons in 4 mm gap cuvees 1 Kit

US & Canadian Customers

phone 508.893.8999

fax 508.429.5732

e-mail [email protected]

website www.btxonline.com

International Customers

To locate a distributor

near you, please visit our

website at www.btxonline.com

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