Hanna Instruments HI 84432 Manuel utilisateur

Instruction Manual

HI 84432

TITRATABLE ACIDITY

MINITITRATOR & pH METER

for Fruit Juices

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Dear Customer,

Thank you for choosing a Hanna product. This manual will provide you with the necessary

information for the correct use of the instrument. Please read it carefully before using the meter.

If you need additional technical information, do not hesitate to e-mail us at [email protected].

This instrument is in compliance with directives.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent

of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION................................................................................................. 4

GENERAL DESCRIPTION ........................................................................................................ 4

SPECIFICATIONS ................................................................................................................... 6

PRINCIPLE OF OPERATION .................................................................................................... 7

FUNCTIONAL AND PHYSICAL DESCRIPTION............................................................................. 8

TITRATOR STARTUP ........................................................................................................... 10

SETUP CONFIGURATION MENU .......................................................................................... 11

ELECTRODE PREPARATION .................................................................................................. 15

ELECTRODE CALIBRATION PROCEDURE................................................................................. 16

pH BUFFER TEMPERATURE DEPENDENCE ............................................................................ 20

PUMP TUBE INSTALLATION ................................................................................................. 21

PURGE .............................................................................................................................. 21

PUMP CALIBRATION PROCEDURE ........................................................................................ 23

TITRATION PROCEDURE ..................................................................................................... 26

pH MEASUREMENT ............................................................................................................ 31

TEMPERATURE CALIBRATION PROCEDURE (for technical personnel only) ............................... 35

PC INTERFACE ................................................................................................................... 37

TROUBLESHOOTING GUIDE ................................................................................................ 40

ELECTRODE CONDITIONING & MAINTENANCE ...................................................................... 41

ACCESSORIES .....................................................................................................................42

WARRANTY ........................................................................................................................43

Please examine this product carefully. Make sure that the instrument is not damaged. If any

damage occurred during shipment, please notify your Dealer.

Each HI 84432 minititrator is supplied complete with:

•HI 1131B pH electrode

•HI 7662-M Temperature probe

•HI 84432-50 Titrant solution (100 mL)

•HI 84432-55 Pump calibration solution (100 mL)

•HI 7061M Cleaning solution (230 mL)

•HI 70300M Storage solution (230 mL)

•HI 7071 Filling solution (30 mL)

•HI 7004M pH 4.01 buffer solution (230 mL)

•HI 7007M pH 7.01 buffer solution (230 mL)

•HI 70082M pH 8.20 buffer solution (230 mL)

• HI 740142 One 1mL syringe

• HI 740037 Two 20 mL beakers

• Two 100 mL beakers • Tube set with dispensing tip

• Stir bars (small 2 pcs.) • 12 Vdc power adapter

• Instruction manual • One medium eye dropper

Note: Save all packing material until you are sure that the instrument works correctly. Any

defective item must be returned in its original packing.

PRELIMINARY EXAMINATION

The HI 84432 is an easy to use microprocessor-based automatic minititrator and pH meter designed

for the rapid and accurate analysis of Total Titratable Acidity in fruit juices. By eliminating subjective

factors including color indicators, errors in mathematical calculations or erratic titrant additions from

the measurement, the HI 84432 makes Total Titratable acidity analysis precisely. This will quickly

become a valuable acidity analysis tool of fruit juices.

The instrument benefits from Hanna’s many years of experience as manufacturer of quality analytical

instruments. A clear and well-designed user interface makes the instrument intuitive and simple to

use. A dedicated HELP key aids in set-up, calibration, status and troubleshooting.

By simply pressing the START key, the HI 84432 automatically starts pump operation and titrates

the sample to the end point. The HI 84432 has a simple and accurate peristaltic pump to ensure the

best accuracy and repeatability. By performing pump calibration with the Hanna standard provided,

the instrument accuracy is assured.

The instrument employs a powerful and effective built-in algorithm to analyze the pH response to

determine the exact pH endpoint, then uses this to make the necessary calculations.

GENERAL DESCRIPTION

The Titratable Acidity determination is instantaneously displayed in selected measurement units

on the large dot matrix display. The instrument is ready for the next analysis immediately.

Other features:

• Log on demand up to 100 samples (50 for pH measurement; 50 for titration results)

• GLP feature, to view last calibration data for pH electrode and pump

• PC interface via USB connection

MEASUREMENT SIGNIFICANCE

Titratable acidity of fruit juices measures the concentration of titratable hydrogen ions contained in

the fruit juices samples, by neutralization with strong base solution at fixed pH. This value includes

all the substances of an acidic nature in the fruit juice: free hydrogen ions, organic acids, acid salts

and cathions. Because the organic acid is the most acidic component of the fruit juices that react

with strong bases solutions, the titratable acidity is usually expressed as g/L or g/100mL of the

predominant acid contained:

•Citric acid is present in many fruits species.

•Tartaric acid is essentially found in grapes.

•Malic acid is present in many fruit species, sometimes together with citric acid or tartaric

acid in unripe grapes.

Titratable acidity of fruit is an important parameter in determining fruit maturity.

The HI 84432 minititrator uses a method based on the Official Methods of Analysis of AOAC International.

The fruit juice is titrated with a sodium hydroxide solution until the end point at 8.2 pH is reached

(determined by potentiometric method).

Additionaly the HI 84432 has a built-in pH meter for pH measurement (electrode and meter must

be calibrated).

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REQUIRED REAGENTS

Code Description Quantity/Test

HI 84432 - 50 Titrant 1 mL

HI 84432 - 55 Pump Calibration Solution 2 mL

SPECIFICATIONS

Titrator Range Titratable acidity:

g/100 mL as citric acid : 0.20 - 8.00% CA

g/100 mL as tartric acid : 0.23 - 9.30% TA

g/100 mL as malic acid : 0.21 - 8.30% MA

Resolution Titratable acidity:

0.01%

Accuracy 5% of reading ± 0.05

Titration method Acid-base titration

Principle End point titration : 8.20 pH

Pump debit 0.5 mL/min

Stirring speed 600 rpm

Log data Up to 50 samples

pH meter pH meter -2.0 to 16.0 pH / -2.00 to 16.00 pH

pH Resolution 0.1 pH / 0.01 pH

pH Accuracy ± 0.01 pH

pH Calibration 1, 2 or 3 calibration points;

3 available buffers (4.01; 7.01; 8.20)

Temperature manual or automatic from

compensation -20 to 120 °C (-4 to 248 °F)

Log data Up to 50 samples

Temperature Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)

Resolution 0.1 °C

Accuracy ±0.4 °C without probe error

Electrode HI 1131B (included)

Temperature Probe HI 7662-M (included)

Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing

Power supply 12 Vdc power adapter

Dimensions 208 × 214 × 163 mm (8.2×8.4×6.4”) (with beaker)

Weight 2200 g (77 oz.)

PRINCIPLE OF OPERATION

Fruit juice acidity is determined by neutralization of the all available hydrogen ions present in the

sample, with a strong base solution:

H++HO-H2O

In an ideal solution, the end point of an acid titration corresponds stoichiometrically to the complete

neutralization of the acids present.

Using a pH electrode allows an end point at 8.2 pH to be automatically detected. End point

detection by potentiometric method is more objective than the end point detection using the

indicators.

The HI 84432 minititrator is designed to determine the acidity of fruit juices by potentiometric

titration. The result of the titration are displayed in % (g/100mL) of the predominant acid (citric,

malic or tartaric acid).

For precise results is important to know the sample volume and the titrant volume and concentration.

It is important to titrate fresh samples of the fruit juice. For increased measurement precision keep

the fruit juices in caped vessels (avoid prolonged exposure to air). Also avoid refrigerating the juice

as significant portion of the tartaric acid (ex. grapes juice) will precipitate out and the results

obtained will be lower then expected results.

Titratable Acidity in fruit juices, as performed on the HI 84432 minititrator, utilizes a simple sample

preparation, a high quality peristaltic dosing pump for titrant, potentiometric endpoint detector and

instantaneaous computations. To maintain the high precision of the titrator, a simple pump calibration

procedure is required. The pump calibration involves the analysis of a known volume of a known

solution (standard provided) and compensates for changes in pump dosing that may occur due to

many factors including tube stretching or aging. This procedure should be performed regularly.

OVERHEAD VIEWOVERHEAD VIEW

OVERHEAD VIEW

1. Titrant bottle

2. Graphic LCD

3. Keypad

4. Dedicated guide tube

for Temperature Probe

5. Electrode holder

6. Dedicated guide tube for titrant tip

7. Peristaltic pump tube

FRONT VIEWFRONT VIEW

FRONT VIEW

REAR VIEWREAR VIEW

REAR VIEW

8. Temperature probe

9. pH Electrode

10. Beaker

11. BNC electrode connector

12. Temperature probe socket

13. USB connector

14. Power switch

15. Power adapter connector

16. Peristaltic pump

FUNCTIONAL AND PHYSICAL DESCRIPTION

ESC - used to leave the current screen and to return either to the previous screen or to the

main screen. When pressed while modifying a parameter within the SETUP menu,

exits parameter without changing it.

- used to modify the parameters’ values, to scroll the information displayed while

viewing a help or to move between the options from the instrument’s SETUP

HELP - used to access/leave the instrument’s contextual help

MENU - used to enter SETUP,Recall or GLP selection menu, while instrument is in pH or

Titration main screen

STIR - used to start/stop the stirrer.

Note: The stirer starts automatically during pump calibration and titration and cannot be

stopped by pressing STIR key.

1. Current time and instrument mode information (pH meter or Titrator)

2. pH electrode condition information

3. Main reading information

4. Instrument status information

GUIDE TO INDICATORS

During the instrument’s operation information is displayed on the LCD.

Displayed icons:

Unstable reading.

Stirrer on.

Pump running.

Parameter can be changed.

KEYPAD FUNCTION AND INDICATORS

PERISTALTIC PUMP

Peristaltic pumps are self priming. Liquid never contacts the pump components. The titrant tubing

is pressed along the rotating rollers of the pump. The rollers compress the tubing, driving the

titrant to the dispensing tip.

TITRATOR STARTUP

This is a general outline of the steps required to make a titration. The following sections expand

upon each section.

• Place the instrument on a flat table. Do not place the instrument in direct sun light.

• Connect the power adapter to the instrument.

• Turn the instrument ON using the power switch from the rear panel of the instrument.

• Set up the instrument. See the “Setup Configuration Menu” section for details. Pay attention

to the Acidity Unit.

• Connect the pH sensor and temperature probe to the instrument.

• Calibrate the pH electrode. At least a single point calibration is necessary for titration.

• Place the peristaltic pump tube on the pump (inlet tube is connected with the reagent bottle,

outlet tube is connected with the dosing tip). See the “Pump Tube Replacement” section for

the procedure.

• Remove the reagent bottle cap and replace with the bottle cap with tubes. Place the reagent

bottle in the appropriate place on the titrator top.

• Purge the titrant.

• Calibrate the pump.

• Prepare the sample.

• Run a titration and log sample results.

5. Functional key area

6. Indicates that the displayed value can be changed using ARROW keys

7. Temperature value (Manual or Automatic)

8. pH temperature compensation mode (Manual or Automatic)

9. Stirrer and reading status area

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