Grant-bio PCMT Manuel utilisateur

Thermo-shaker

with cooling

PCMT

Operating instructions

Thermo-shaker PCMT Version 1.01 - January 2013

Page 1 Operating Instructions

Contents

1 Safety

2 General Information

3 Getting Started

4 Operation of PCMT

5 Calibration

6 Specifications

7 Guarantee and service

Thermo-shaker PCMT Version 1.01 - January 2013

Page 2 Operating Instructions

1. Safety

The following symbols mean:

Caution: Make sure you have fully read and understood the present Manual

before using the equipment. Please pay special attention to sections

marked by this symbol.

Caution: Surfaces can become hot during use!

GENERAL SAFETY

Use only as specified in the operating instructions provided. The unit should not be used if it

has been dropped or damaged.

The unit must be stored and transported in a horizontal position (see package label).

After transport or storage allow the unit to reach room temperature (2-3 hrs) before

connecting to the mains. Use only standard quality tubes.

Before using any cleaning or decontamination method except those recommended by the

manufacturer, check with the manufacturer that the proposed method will not damage the

equipment.

Do not make modifications to the design of the unit.

ELECTRICAL SAFETY

Connect only to the mains with a voltage corresponding to that on the serial number label.

Use only the external power supply unit provided with this product.

Ensure that the mains switch and external power supply are easily accessible during use.

Do not plug the unit into the mains outlet without grounding, and do not use extension lead

without grounding.

Before moving the unit, disconnect it from the mains outlet.

To turn off the unit, disconnect the external power supply from the mains outlet.

If liquid is spilt inside the unit, disconnect it from the external power supply and have it

checked by a qualified repair and maintenance technician.

DURING OPERATION

Do not leave the operating unit unattended.

Do not impede the platform motion.

Do not operate the unit in environments with aggressive or explosive chemical mixtures.

Do not operate the unit if it is faulty or been incorrectly installed.

Thermo-shaker PCMT Version 1.01 - January 2013

Page 3 Operating Instructions

For indoor use only.

Intended for laboratory use only.

Do not check the temperature by touch. Use a thermometer.

BIOLOGICAL SAFETY

It is the user's responsibility to carry out appropriate decontamination if hazardous material

is spilt on or inside the equipment.

Thermo-shaker PCMT Version 1.01 - January 2013

Page 4 Operating Instructions

2. General Information

Thermo-Shaker PCMT is an ideal instrument for intensive mixing of samples with heating or cooling.

Heating (up to +100°C), cooling (up to 15°C below room temperature - RT) and mixing modes can be

used both simultaneously and independently i.e. the unit is three products in one:1)ashaker,2)a

thermostatand3)athermo-shaker.

Features of the PCMT:

•Quick to reach the specified mixing speed and set temperature.

•Stability of maintaining the set temperature in a wide range throughout the Thermo-shaker’sblock

surface;

•Withthehelpofthetemperaturecalibrationfunction,theusercancalibratethe unit approx. ±6% of

the selected temperature to compensate for differences in thethermalbehavioroftubesfrom

differentmanufacturers;

•LCD display indicates preset and current values of temperature, speed and timeofoperation;

•Quietmotoroperation,compactsize,prolongedservicelife.

Cooling system–Peltierelements.

Heating system–flatheatingelement(12V).

Mixingtype–orbital.

PCMT isapplicablein:

•DNAanalysisforDNAextractionandfurthersamplepreparation;

•Biochemistryforstudiesofenzymaticreactionsandprocesses;

•Metaboliteextractionfromcellmaterials.

Therearefiveheatingandcoolingblocksavailable,includingablockwithaplastic lidforPCR-plates.

Allblocksare interchangeable andcan be quickly and easilyinstalled.

Thermo-shaker PCMT Version 1.01 - January 2013

Page 5 Operating Instructions

3. Getting started

3.1 Unpacking

Remove packing materials carefully, and retain for future shipment or storage of the unit.

Examine the unit carefully for any damage incurred during transit. The warranty doesnot

coverin-transitdamage.

Attention! Automatic balancing system in this product produces light metal-like noise when moving

the unit which is likely to be heard during unpacking. It is normal occurrence and does not

indicate a fault.

3.2 The Thermo-shaker PCMT set includes:

•Thermo-shaker PCMT ....................................................................................1 piece

•Microtube block ...............................................................................................1 piece

•Spare rubber belt ..........................................................................................2 pieces

•External Power Supply.....................................................................................1 piece

•Operating instructions; CE Certificate...............................................................1 copy

3.3 Optional blocks

•HC32 block...................................for 20x0.2 ml microtubes + 12x1.5 ml microtubes

•HC24N block.......................................................................for 24x1.5 ml microtubes

•HC18 block .....................................................for 20x0.5 ml + 12x1.5 ml microtubes

•HC24 Block.........................................................................for 24x2.0 ml microtubes

•HC96 Block..............................for 96-well PCR microplate (semi-skirted, unskirted)

3.4 Set up:

• Place the unit upon even horizontal non-flammable surface away from any flammable

materials, remove protective film display.

• To provide optimum ventilation ensure clearance around the device - 20 cm on side

faces.

• Plug the external power supply into the socket at the rear side of the unit and position

the unit so that there is easy access to the power switch and external power supply.

3.5 Installing blocks (if block is not installed at the moment of delivery)! Disconnect from the

mains!).

Connect the plug to the contact terminal on the underside of the block. Align the block

so that the warning label is facing the front of the unit and secure with the four knurled

screws.

3.6 Changing blocks

Disconnect the external power supply from the

mains. Remove the four knurled screws,

disconnect the plug. Select the new block.

Connect the plug.

Thermo-shaker PCMT Version 1.01 - January 2013

Page 6 Operating Instructions

Align the block so that the warning label is

facing the front of the unit and secure with the

four knurled screws.

3.7 Note! When installing the HC96 block, drive one by one 4 screws for several turns and fix

them with a hex-key (included in the HC-96 thermo-block set).

Fig.1 Thermo-block connection

3.8 Changing Blocks

Disconnect the external power supply unit from the device.

Remove the four knurled screws (fig.2/1); disconnect the plug (fig.1/1).

Select the new block and install it as point 3.5

Fig.2 Thermo-block set up

Thermo-shaker PCMT Version 1.01 - January 2013

Page 7 Operating Instructions

4. Operation of PCMT

Recommendations during operation

•Please check that the tubes/microwell plates are thermo resistant. Don't heat the

tubes/microwell plates over the melting point of the material they are made of (use thermo

resistant polypropylene tubes). Remember that thin-walled tubes have a higher thermo

conductingfactor.

• Under high temperatures (> 85°C) tube caps can open, causing evaporation of the sample or a

potential health risk when working with infected material. To prevent such occurrences it is

recommendedtousetubeswithcaplockofSafe-Lock® t y p e .

• Forefficientmixingitisrecommendedtofilltesttubesupto75%ofthetube specifiedvolume.

4.1 Connect the external power supply to the grounded mains and switch ON the power switch

located on the rearpaneloftheunit.

4.2 The display will turn on with the upper line (Set) showing time, RPM and temperature set

earlier and the lower line (Actual) showing current readings of the same parameters

(thermo block temperature °C, which automatically starts rising according to the temperature

set in the upper line). The time to reach the set temperature will depend on the initial

temperature.

Setting the PCMT

Use the readings in the upper line of the display (Set), while setting the required parameters.

Fig.3 Control Panel

Timeset(TIME)

4.3 With the help of “▲” and “▼“ keys Fig. 3/1 set the required working time interval in hours and

minutes (increment - 1 min). If the key is pressed for more than 3 seconds, the increment

becomes bigger.

Speed set (RPM)

4.4 With the help of “▲” and “▼“ keys Fig. 3/2 set the required speed (increment 10 RPM). If the

key is pressed for more than 3 seconds, the increment becomes bigger.

Temperature set (T, ºC)

4.5 With the help of “▲” and “▼“ keys Fig. 3/3 set the necessary temperature (increment

0.1ºC). If the button is pressed for more than 3 seconds, the increment becomes bigger.

1 2 3 4

00:00 1000

35.0

STOP 000

35.0

hr:min

Thermo-shaker PCMT Version 1.01 - January 2013

Page 8 Operating Instructions

The set parameters can also be changed during operation.

Caution! The timer ending does not stop the heating. Heating can only be turned off when it is

set below 25°C (The display will show OFF –T, °C –set point)

Program execution

After the PCMT has reached a stable temperature (when the set and current temperature

readings become the same):

4.6 Insert tubes into the block or place the microwell plate on the block and close the lid.

4.7 Press the RPM-RUN/STOP key Fig. 3/4 the block will start rotation and the timer indicator will

start counting up the time interval (with 1 min precision).

Note! If the rotation speed is set to zero, pressing RPM-RUN/STOP key starts the timer but the

block does not move.

Attention! Automatic balancing system in this product produces light metal-like noise when moving

the unit which is likely to be heard during acceleration/deceleration. It is normal

occurrence and does not indicate a fault.

4.8 At the end of the program (after the set time elapses) the block motion stops and the timer

shows the flashing reading STOP accompanied by the repetitive sound signal until the RPM-

RUN/STOP key is pressed.

4.9 If the working time is not set (or is re-set) and the timer indicator in the upper line shows

00:00, pressing the RPM-RUN/STOP button will start continuous operation of the device with

the countdown timer in the lower line (Actual), until the RPM-RUN/STOP button is pressed

again.

4.10 If required, it is possible to restart the timer when it is running. Press the TIME-RUN/STOP key

once Fig. 3/5 to stop the timer. Press the TIME-RUN/STOP key again to restart the timer.

4.11 The block motion can be stopped at any time by pressing the RPM-RUN/STOP key. In this

case the program and the thermo block motion stops and timer is set back to zero switching

into the STOP mode. Press the RPM-RUN/STOP key to repeat the operation with the same

time and speed.

4.12 At the end of operation turn OFF the unit with the power switch at the rear panel and

disconnect the external power supply from the mains.

Thermo-shaker PCMT Version 1.01 - January 2013

Page 9 Operating Instructions

5. Calibration

5.1. The operating temperature of the device is pre-calibrated at the factory (the calibration

coefficient is 1.00) which is measured by an external sensor installed in the heating block.

5.2. To enter the calibration coefficient, hold the TIME-RUN/STOP key (Fig. 3/5) for more than 8

sec to activate calibration mode. The calibration coefficient will be shown on the display (Fig.

5/1).

5.3. Set 1.000 value using the ▲ and ▼ keys (Fig. 3/3) as shown on Fig. 5/1 to restore the factory

settings.

5.4. PresstheRPM-RUN/STOPkeyoncetoexitthecalibrationmode.

Calibration procedure

5.5. Install an independent temperature sensor (thermometer 0.5 C accuracy) into the tubes, placed

into the block,ordirectlyintotheheatingblockspecialsensorsocket.

5.6. Set therequiredtemperatureinoperationmode(e.g.40°C).

5.7. Aftertheunitreachesthesettemperature(whenthesetand actual temperature readingsareequal)

leavetheunitfor30minforthermalstabilization.

5.8. Let us assume that the reading of the independent temperature sensor is 39°C, but the display’s

actual temperature is 40 °C (Fig.4). Then it is necessary to add 1°C correction.

Fig.4 Control panel in operation mode

5.9. Hold down the TIME-RUN/STOP key (Fig. 3/5) for more than 8 sec. to activate calibration

mode. Thefollowingparameterswillbeshownonthedisplay(Fig.5)

Fig.5 Control panel in calibration mode

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