Fisher Bioblock Scientific Stemi 1000 Manuel utilisateur

Stemi 1000/2000/2000-C

Stereo Microscopes

Operating Manual

Unless expressly authorized, forwarding and duplication of this document, and the

utilization and communication of its contents are not permitted. Violations will entail an

obligation to pay compensation.

Number of this

manual: B 40-002 e

Date of issue: 12/98



Issued by: Carl Zeiss

Mikroskopie

D-07740 Jena

Telephone (**49) 03641 / 64-1616

Telefax: (**49) 03641 / 64-3144

Internet: [email protected]

http://www.zeiss.de

Knowledge of this manual is required for the operation of the instrument. Would you therefore

please make yourself familiar with the contents of this manual and pay special attention to hints

concerning the safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service.

Stemi 1000, Stemi 2000, Stemi 2000-C

B 40-002 e 12/98 I

TABLE OF CONTENTS

Page

Title

Table of contents I. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

List of figures III. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Note IV. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

General view V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Notes on device safety VII. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

1 DESCRIPTION

1.1 Designation, purpose 1-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

1.2 Description of the units 1-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

1.3 Technical data 1-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

1.4 Overview of modules 1-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

1.5 Function elements 1-29. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

2 OPERATION

2.1 Unpacking and setting up 2-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

2.2 Commissioning 2-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

2.2.1 Working with accessories 2-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

3 FLUORESCENCE CONTRAST WITH STEREO MICROSCOPES

3.1 FL S (Fluorescence for Stereo microscope) configuration 3-2. . . . . . . . . . . . . . . . . .

3.1.1 Name and intended application 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

3.2 Configurations 3-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

3.2.1 FL S configuration with KL 1500 electronic (not suitable for UV excitation) 3-4. . .

3.2.2 FL S configuration with HBO 50 or HBO 100 3-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . .

3.2.3 FL S configuration with LUMATEC HBO 200 3-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C

12/98 B 40-002 e

4 CARE AND TROUBLESHOOTING

4.1 Care 4-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

4.2 Troubleshooting and service 4-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Page

ANNEX

– List of abbreviations A-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

– Certification in accordance with DIN ISO 9001/EN 29001/EN 46001 A-5. . . . . . . .

– EC conformity declaration A-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C

B 40-002 e 12/98 III

LIST OF FIGURES Page

Figure 1-1 Principal assemblies of the Stemi 2000-C 1-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-2 Optical schematic of the Stemi 2000-C 1-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-3 Basic microscope equipment 1-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-4 Eyepieces/eyepiece cups/reticules/attachment systems/specimen

measuring plate 1-10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-5 Stands 1-12. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-6 Specimen stages 1-14. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-7 Halogen lighting systems for vertical illumination 1-16. . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-8 Cold light sources for vertical illumination 1-18. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-9 Halogen lighting systems for transmitted illumination 1-20. . . . . . . . . . . . . . . . . . . . . .

Figure 1-10 Cold light sources for transmitted illumination 1-22. . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-11 Polarisation systems 1-24. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-12 Documentation systems using photo cameras 1-26. . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-13 Adapters for video cameras 1-28. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 1-14 Function elements of the Stemi 2000-C 1-30. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-1 Stemi 2000-C packing unit in city box 2-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-2 Unpacking 2-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-3 Setting up the Stemi 2000-C 2-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-4 Adjusting the Stemi 2000-C 2-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-5 Inserting a graticule 2-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-6 Fitting an attachment system to the Stemi 2000 2-4. . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-7 Mounting the polariser 2-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-8 Mounting a reflex camera 2-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 2-9 Mounting the microscope camera MC 80 for 35 mm photography 2-7. . . . . . . . . .

Figure 2-10 Mounting a video camera 2-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C

12/98 B 40-002 e

Page

Figure 3-1 Stemi 2000 with KL 1500 electronic 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-2 Stemi SV 6 with HBO 100 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-3 Stemi 2000 with HBO 50 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-4 Stemi SV 11 with LUMATEC HBO 200 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-5 FL S configuration with KL 1500 electronic 3-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-6 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-7 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-8 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-9 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-10 FL S configuration with HBO 50 or HBO 100 3-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-11 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-12 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-13 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-14 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-15 FL S configuration with LUMATEC HBO 200 3-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-16 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-17 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-18 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-19 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-20 3-10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 3-21 3-10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Figure 4-1 Lamp replacement 4-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C

B 40-002 e 12/98 V

NOTE

•The figures integrated in the text each have a figure number and a caption, e.g. ”Fig. 2-8”signifies:

the figure in Section 2 with the serial number 8. In each figure, details discussed in the text are

assigned a reference line marking and an item number. In the running text, ”T2 adapter (2-8/3)”: in

Fig. 8 of Section 2, the T2 adapter is marked with the item number 3.

•Refer to the annex for explanations of the abbreviations.

•This instruction manual refers to the Stemi 1000, Stemi 2000 or the Stemi 2000-C microscope

versions (see Page 1-9) and their options.

Apply this manual analogously when working with other variants of the unit.

Stemi 1000, Stemi 2000, Stemi 2000-C

12/98 B 40-002 e

GENERAL VIEW

(Basic unit)

Stemi 1000 Stemi 2000

Stemi 2000-C

Stemi 1000, Stemi 2000, Stemi 2000-C

B 40-002 e 12/98 VII

NEW MODEL

We have included a further Stemi model in our line of Greenough-type stereo microscopes.

In response to many requests received from customers, the

Stemi 2000-CS 45 50 55

is now also available as a brand-new item in addition to the Greenough-type stereo microscopes

Stemi 1000, Stemi 2000 and Stemi 2000-C.

Unlike the Stemi 2000-C, the Stemi 2000-CS (”C”for ”Camera”and ”S”for ”Splitter”) features a

fixed camera port

with a 50/50 % beam splitting ratio, i. e. the camera port has no switching lever.

Unlike the Stemi 2000-C, the Stemi 2000-CS therefore provides the benefit of

simultaneousstereoscopic viewing

while taking photographs or working in the monitor mode.

All configurations or accessory components are identical to those of the other Stemi’s.

Stemi 1000, Stemi 2000, Stemi 2000-C

12/98 B 40-002 e

VIII

NOTES OF DEVICE SAFETY

The Stemi 1000, Stemi 2000 or the Stemi 2000-C stereo microscope including original accessories or

options must only be used for the microscopy applications described in this operating manual.

The manufacturer cannot assume any liability for any other applications, possibly also involving indi-

vidual modules or single parts. This also applies to all service or repair work that is not carried out by

authorised service personnel. All guarantee/warranty claims also expire for all those parts that were not

directly affected by repair.

Particular attention must be paid to the following warning notes:

The Stemi was designed and tested in conformity with the IEC Publication 1010-1 ”Safety

requirements for electrical measuring devices, control equipment and laboratory apparatus”and

was delivered in a safe state. This operating manual contains information and warnings which must

be followed by the operator.

The Stemi is a light microscope conceived in accordance with the latest scientific and technical

knowledge for visual, microphotographic and video analysis of microscopic specimens. The unit

must only be used for the intended purpose. It is not intended for continuous unsupervised opera-

tion!

The Stemi has no special facilities to protect against samples that have caustic, toxic, radioactive

or other effects that are damaging to health.

Users must check whether the available power supply agrees with the value specified on the rating

plate of the respective power supply unit.

The power supply unit belonging to the Stemi is a protective class II unit.

Internal parts can carry hazardous voltages when a power supply unit is connected to the mains.

The power supply unit must therefore be disconnected from the mains before opening it.

Make sure that only those fuses are used as spares for the respective power supply unit that are

actually intended for the prescribed rated current and the specified version. It is forbidden to use

provisional fuses or to short-circuit the fuse holders.

Modifications to the unit to keep in line with technical progress are always reserved.

Ce manuel convient aux modèles suivants

Table des matières

Manuels Microscope populaires d'autres marques

Thermo Scientific

Thermo Scientific Nicolet iN10 Mode d’emploi

National

National DC3-163 Manuel utilisateur

KERN Optics

KERN Optics OPE-1 Manuel utilisateur

OPTIKA MICROSCOPES

OPTIKA MICROSCOPES B-150D Manuel utilisateur

Prior

Prior Lux POL Manuel utilisateur

Opitec

Opitec Smartscoop 115615 Manuel utilisateur

KERN

KERN OPO-1 Manuel utilisateur

Topcon

Topcon SP-1P Manuel utilisateur

Motic

Motic PA43 Manuel utilisateur

Leica

Leica DVM5000 Guide de l'utilisateur

MshOt

MshOt MZ62 Manuel utilisateur

KERN Optics

KERN Optics OZR-5 Manuel utilisateur

VWR

VWR VisiScope 384 Series Manuel utilisateur

Nikon

Nikon ECLIPSE E200 POL Manuel utilisateur

Leica

Leica DI C800 Manuel

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manuel utilisateur

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manuel utilisateur

Olympus

Olympus SZ61 Manuel utilisateur