Fein Optic IRB20 Manuel utilisateur
Fein
IRB20 Inverted Microscope User’s Manual
info@feinoptic.com
1Fein
IRB20 Microscope Components
Eyepieces Condenser
Lamp Housing
Nosepiece
Mechanical
Stage
Rheostat
On/O
Power
Siedentopf
Trinocular Head
C-Mount Adapter Phase
Slider
Beam Splitter
Coarse Focus
Fine Focus
Stage
X-Y Control
2
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IRB20 Microscope Components
Mechanical
Stage
On/O
Power
Stage
X-Y Control
1. Eyepiece
2. Siedentopf Trinocular Head
3. Phase Contrast Slider
4. Aperture Diaphragm Lever
5. Filter Slider
6. Lamp Housing
7. Condenser
8. Round Insert on Stage
9. Fixed Stage
10. Carrying Handle
11. Objective Lens
12. Nosepiece
13. Power Socket
14. Fuse Box
15. Power Switch
16. Surplus Water Plate
17. Mechanical Stage X-Y Knob
18. Light Rheostat Control
19. Coarse & Fine Focus Control
20. Attachable Mechanical Stage
21. Beam Splitter Knob
IRB20 Inverted Microscope Assembly
3Fein
• Once the microscope is assembled, turn on the main power source.
• Place the sample on the stage.
• Move the 10x objective lens into the light path.
• Focus on the sample using the coarse focus and then the ne focus.
• Adjust the brightness using the rheostat control.
• Adjust the interpupillary distance and the eyepiece diopter.
• Adjust the aperture diaphragm.
• Once the sample is in focus, lters may be placed in the optical path.
IRB20 Inverted Microscope Operation Instructions
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Do not shake or drop the microscope.
Do not expose the microscope to direct sun, high temperatures, high humidity, dust,
or damp environments. Use a at work surface.
When moving the microscope use
both hands, holding the handle at the
back (1) and the frame at the front (2)
as shown in the image at right.
Set the power switch to o “O” before
replacing a bulb or fuse, and wait until the
lamp is cool. The power switch is located
on the side of the microscope. Microscope
uses 5W LED bulb. (Part # M40-001).
Voltage range of 100~240V (47-63Hz) is supported.
Turn the rheostat control knob (1) to the
minimum and turn the power switch (2) to
the on “I”position. Rotate the rheostat knob
to increase or decrease the brightness of the
illumination.
Adjust the aperture diaphragm lever (1) to
obtain the best image contrast.
IRB20 Inverted Microscope Operation Instructions
5Fein
Place the lter (1) inside
the lter holder (2), and
insert into the lter slot on
the condenser.
The tension of the coarse focusing knob can be
adjusted by holding the tension adjustment ring (1)
and rotating it. Turning the adjustment ring clockwise
will increase the focus tension and turning it counter-
clockwise will decrease the tension.
If the stage is drifting (falling) out of focus, tighten the
focus tension ring (clockwise) to create more tension
and keep the stage from drifting.
Do NOT rotate the coarse & ne focus knobs in
opposite directions at the same time!
Adjust the eyetubes to set the interpupillary
distance for your eyes. If multiple people
use the microscope daily, note the number
of your interpupillary setting for ease of
future setup.
Set both eyepieces to“0” scale on the
diopter. Look through the microscope
with only one eye and focus. Now
close the eye you just looked through
and look through the other eyepiece
with the other eye only. If the sample is
out of focus, adjust the diopter on that
eyepiece until it is clearly in focus.
IRB20 Inverted Microscope Operation Instructions
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The eyepieces can be locked in place with
a set screw (1). To change the eyepiece,
loosen the set screw and pull the eyepiece
out of the eyetube.
FIXED STAGE:
Insert the round plate (1) into the xed
stage.
Install the stage clips (2) into the slot
on the xed stage.
MECHANICAL STAGE:
Insert the round plate (1) into the stage.
Install attached mechanical stage (2) with screws (3) provided.
Place petri dish or stage inserts (4) into stage as needed.
IRB20 Inverted Microscope Bulb Replacement
7Fein
Turn o and unplug the microscope prior to
changing the bulb.
1. Remove the screw with the allen wrench.
2. Lift the illumination cover.
3. The bulb is located at the front of the housing.
Once the cover is removed, unscrew the two
screws in the image shown below that lie on
either side of the wires leading to the bulb.
IRB20 Inverted Microscope Bulb & Fuse Replacement
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When you lift the bulb housing be careful
since the wires are still connected. In order
to replace the bulb remove the solder joints
from the wires connected to the bulb.
Unscrew the 2 screws holding the bulb to
the housing. Screw the new bulb into the
housing. Re-solder the wires back onto the
bulb in the same positions they were in
prior to bulb replacement.
Replacement Bulb: M40-001 (5W LED).
FUSE REPLACEMENT:
Turn o and unplug the microscope prior to fuse replacement.
The fuse box is installed below the power socket.
Fuse: 5x20mm, 2A, 250V.
IRB20 Inverted Microscope Phase Contrast Setup
9Fein
Ensure phase slider is right side up and slide into the condenser.
Place phase objective in the light path and put the phase slider in the position that
matches the phase objective magnication.
Remove the eyepiece and insert the phase centering telescope. Use the phase
centering screws to align the bright and dark rings as shown in the illustrations
below. Image at left shows what the rings might look like prior to alignment. Image
at right shows once they are aligned.
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