Excelitas Technologies Pco.edge 10 bi LT Manuel utilisateur

pco.edge 10 bi LT

the next level sCMOS camera

user manual

Excelitas PCO GmbH asks you to carefully read and follow the instructions in this document.

For any questions or comments, please feel free to contact us at any time.

telephone: +49 (0) 9441 2005 50

fax: +49 (0) 9441 2005 20

postal address: Excelitas PCO GmbH

Donaupark 11

93309 Kelheim, Germany

email: [email protected]

web: www.pco.de

pco.edge 10 bi LT user manual 1.0.0

Released August 2023

©Copyright Excelitas PCO GmbH

This work is licensed under the Creative Commons Attribution-NoDerivatives 4.0 International License. To

view a copy of this license, visit http://creativecommons.org/licenses/by-nd/4.0/ or send a letter to Creative

Commons, PO Box 1866, Mountain View, CA 94042, USA.

pco.edge 10 bi LT

pco.edge 10 bi LT user manual 1.0.0 2

Contents

1 Safety 4

2 System components 5

2.1 Camerapco.edge10biLT ....................................... 7

2.2 Lensmountadapters .......................................... 8

2.2.1 C-mountadapter......................................... 8

2.2.2 F-mountadapter......................................... 9

2.2.3 TFL-mountadapter ....................................... 10

2.3 Cables................................................... 10

2.4 MachVissoftware ............................................ 11

3 Camera overview 12

3.1 Camerainterfaces ............................................ 12

3.2 Hardwaresetup ............................................. 12

3.3 Dataandpowerinterface ........................................ 13

3.4 LEDs.................................................... 13

3.5 Triggerandstatusinterfaces ...................................... 13

4 Operation modes 15

4.1 Triggermodes .............................................. 15

4.2 Acquiremode............................................... 17

5 Operation 19

5.1 Systemrequirements .......................................... 19

5.2 Quickstartguide............................................. 19

6 Camera mechanics 21

6.1 Mechanicaloutlines ........................................... 21

6.2 Mounting ................................................. 22

7 Software 23

7.1 Softwarerequirements.......................................... 23

8 Specifications 24

Appendix 26

pco.edge 10 bi LT

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1 Safety

This camera system is a scientific measuring equipment and is designed for use by technicians,

engineers, and scientists. The camera may only be used according to the instructions of this

manual. Provisions, limitations, and operating conditions stated in this manual must be respected.

Unauthorized modifications and alterations of the device are forbidden for safety reasons.

DAMAGED POWER CABLE OR POWER PLUG: Danger to life due to electric shock. Each time

the camera is used, check the power cable for damage.

ELECTRIC SHOCK WARNING DUE TO VOLTAGE PARTS INSIDE: Risk of injury due to electric

shock. Never slide any items through slits or holes into the camera.

MOISTURE: Risk of injury due to electric shock if moisture enters the camera. To avoid the risk

of water condensation, protect the camera against extreme changes of ambient temperature.

TRIPPING HAZARD: Risk of injury from tripping over loose cables. Never position the cable in a

way that it could become a tripping hazard.

HUMIDITY, DUST OR RADIATION: Humidity, dust or X-rays could damage the camera. Never

operate the camera in humid or dusty environments or in places with high levels of X-ray

radiation.

SHOCK AND VIBRATION: To avoid damaging the camera, it must be firmly mounted and

protected against strong shocks or vibrations. Use the camera’s mounting threads to secure it.

LENS MOUNTING: Do not force the lens onto the camera. Screw in the lens gently to avoid

thread damage.

LIQUIDS DAMAGE CAMERA: If liquids have penetrated the device: Switch the camera off

immediately, detach it from power and contact PCO’s customer support.

DAMAGED CAMERA HOUSING: If the camera has been dropped or the camera’s housing is

damaged: Switch the camera off immediately, detach it from power and contact PCO’s

customer support.

pco.edge 10 bi LT Chapter 1

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2 System components

The following standard components are included in your scope of delivery:

Article Picture Details

pco.edge 10 bi LT chapter 3

C-mount adapter chapter 2.2.1

Kaya frame grabber chapter B

Cables (CLHS) chapter 2.3

Power supply chapter 2.3

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These accessories are available for the pco.edge 10 bi LT series:

Article Picture Details

F-mount adapter chapter 2.2.2

TFL-mount adapter chapter 2.2.3

Interface cables

(SMA - BNC) chapter 2.3

Aquamatic III chapter A

All pco.software products can be downloaded for free from the PCO website. For more information

see the PCO homepage.

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2.1 Camera pco.edge 10 bi LT

Thanks for choosing a pco.edge 10 bi LT camera. The pco.edge 10 bi LT is PCO’s next level

sCMOS camera with a new imaging performance. Thanks to its back-illuminated image sensor it

comes with a quantum efficiency of up to 85 % with broad spectrum out to NIR. The sensor

incorporates microlenses and a full pixel height deep trench isolation for crosstalk suppression

resulting in an excellent MTF.

Main Features

•Back-illuminated sCMOS with high MTF

•High resolution 4416 x 2368 pixel

•Low readout noise 1.3e-@ 120 fps

•High dynamic range 15,385 : 1

•temperature-stabilized image sensor

•Fiber-optic data interface

•Pixel size 4.6 µm x 4.6 µm

•Rolling Shutter (RS)

•Up to 85 % quantum efficiency

This document contains important information about the camera pco.edge 10 bi LT.

Please read the instructions given in this document carefully. It contains useful information and

advice to operate the camera in intended way. If there are any questions regarding the pco.edge

10 bi LT camera, please feel free to contact PCO or the local represantative.

Areas of application

Brightfield microscopy Mesoscopy

Fluorescence microscopy High-Speed bright field ratio imaging

Förster resonance energy transfer (FRET) Spinning disk confocal microscopy

High throughput screening of fluorescent

samples

Digital pathology

Single molecule localization

microscopy (SMLM) – PALM, STORM,

dSTORM, GSDIM

High content screening of fluorescent

samples

High throughput screening of fluorescent

samples

Disaster recovery

Lightsheet fluorescence microscopy (LSFM) Image intensifier imaging

PIV: particle image velocimetry Tunnel inspection

Selective plane imaging microscopy (SPIM) Biochip reading

Structured illumination microscopy (SIM) Calcium imaging

Fluorescence recovery after photobleaching

(FRAP)

Ophthalmology

High content screening of fluorescent samples

pco.edge 10 bi LT Chapter 2

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2.2 Lens mount adapters

PCO cameras have an interchangeable lens adapter system to use the camera with different

standard lenses. The adjustment of the lens mount can be done by the user.

2.2.1 C-mount adapter

The C-mount adapter is screwed in the front of the camera and fixed with three allen screws

(marked in red). In order to set the standard back focal distance of the C-mount (17.526 mm),

screw in the C-mount ring until it stands out 1.8 mm from the camera front (see figure 2.1, right).

~1.8 mm

Adjustable

Figure 2.1: C-Mount installation.

Use a suitable lens with a large aperture for fine adjustment.

Follow these steps for the fine adjustment:

•Set the lens focus to infinity and look at an object which is far away.

•Rule of thumb: The distance to the object must be at least 200 times the focal length of the

lens. The further away, the better.

•Then turn the lens mount in until the object is sharp.

•Look at an object which is positioned at the minimum working distance of your lens (as

described in the corresponding datasheet).

•Set the lens focus to the minimum working distance (the opposite direction of infinity).

•Now adjust the lens mount until this object is sharp.

•Repeat the above steps until the focus is consistently sharp in both positions.

CAUTION: The allen screws are designed for a maximum torque of 1 Nm. All torque values in

this manual are recommendations.

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full format

4/3“

1/1“

1“

2/3“

1/1.8“

Ø 23.05 mm

Figure 2.2: sensor size vs. image

circles of various C-mount lenses.

Keep in mind that C-mount lenses can cause shadings at the

edges of large-sized sensors.

Most C-mount lenses can illuminate a maximum image cir-

cle in the range of 9 mm (1/1.8’’) to 22 mm (4/3’’) diameter

only. The pco.edge 10 bi LT has a sensor diagonal of 23.05

mm.

To achieve less shaded images, the ROI function can be used

to limit the resolution of the sensor when using lenses with

smaller image circles than the sensor diameter.

2.2.2 F-mount adapter

The optional F-mount adapter is screwed in the front of the camera and fixed with by the lower

adjustment ring of the adapter. In order to set the accurate back focal distance of the F-mount

(46.50 mm), the position of the adapter can be adjusted. The same procedure as described in

chapter 2.2.1 is applicable.

Back focal

distance fine

adjustment

Automatic

diaphragm

Figure 2.3: F-mount description.

PCO’s proprietary F-mount adapter supports lenses with an automatic diaphragm. It manually sets

the lens aperture by turning the upper adjustment ring of the adapter. F-mount lenses without an

automatic diaphragm can be connected to the mount at the camera, but the aperture cannot be

changed.

Matching lenses with automatic diaphragm

Manufacturer Lens type

Nikon Nikkor Type D, Nikkor Type G

Zeiss ZF.2 (Otus, Milvus, Interlock, Distagon, Planar)

Tamron Type 35 mm: A012, A007, A009, A011, F012, F013

Type APS-C(H): B001, B005, G005

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2.2.3 TFL-mount adapter

The optional TFL-mount adapter is screwed in the front of the camera and fixed with three allen

screws (marked in red). In order to set the standard back focal distance of the TFL-mount (17.526

mm), screw in the TFL-mount ring until it stands out around 1.8 mm from the camera front (see

figure 2.4, right). The TFL mount supports lenses with a maximum sensor diagonal of 28.3 mm.

~1.8 mm

Adjustable

Figure 2.4: TFL-mount installation.

The fine adjustment is done in the same way as described in chapter 2.2.1.

2.3 Cables

The pco.edge 10 bi LT is delivered with an USB interface cable for data transfer and a power

supply cable.

Article Length Description Scope of deliv-

ery

Interface cable 5 m FOL MTP/LC (Female B/4X) BREAKOUT OM4 included

SMA cable 0.3 m Coaxial cable type SMA-BNC available on

request

Power supply 1.5 m Input: 100 - 240 VAC; Output: 24 VDC/ 2.71 A included

Please note that other cables may not be suitable for your camera. If a cable is equipped with a

screwable mount, make sure to attach it to the camera accordingly.

NOTE Only use cables which are approved by PCO. Otherwise full functionality cannot be

guaranteed.

pco.edge 10 bi LT Chapter 2

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