Endress+Hauser KIO1 Caractéristiques techniques

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Brief Operating Instructions

Accessory optics for the Rxn-10

probe

KIO1, KNCO1, KLBIO1, KRSU1,

KRBMO, KRBSL

KA01551C/66/EN/03.22

70201227

Brief Operating Instructions Accessory optics for the Rxn-10 probe

2 Endress+Hauser

Accessory optics for the Rxn-10 probe Brief Operating Instructions 
Endress+Hauser 3 
Table of Contents 
1About this document..................................................................................................5 
1 Disclaimer ........................................................................................................................................................................5 
2 Warnings .........................................................................................................................................................................5 
3 U.S. export compliance....................................................................................................................................................5 
2Basic safety instructions............................................................................................6 
1 Requirements for the personnel ....................................................................................................................................6 
2 Designated use ................................................................................................................................................................6 
3 Workplace safety.............................................................................................................................................................7 
4 Operational safety...........................................................................................................................................................7 
5 Service safety ...................................................................................................................................................................8 
6 Important safeguards......................................................................................................................................................8 
7 Product safety..................................................................................................................................................................8 
3Product description ....................................................................................................9 
1 Immersion optic (KIO1) ..................................................................................................................................................9 
2 Non-contact optic (KNCO1)..........................................................................................................................................10 
3 bIO-Optic (KLBIO1) .......................................................................................................................................................10 
4 Bio multi optic (KRBMO) and bio sleeve (KRBSL) ......................................................................................................11 
5 Raman optic system for single use (KRSU1) ...............................................................................................................13 
4Incoming product acceptance and product identification ......................................14 
1 Incoming acceptance.....................................................................................................................................................14 
2 Product identification ...................................................................................................................................................14 
3 Scope of delivery............................................................................................................................................................14 
5Installation ...............................................................................................................15 
1 Installing immersion optics and bIO-Optics................................................................................................................15 
2 Installing the bio multi optic and bio sleeve system...................................................................................................16 
3 Installing non-contact optics........................................................................................................................................22 
4 Installing the Raman optic system for single use .......................................................................................................23 
6Commissioning.........................................................................................................27 
1 Receipt of optics ............................................................................................................................................................27 
2 Calibration and verification..........................................................................................................................................27 
7Operation..................................................................................................................29 
1 bIO-Optic, bio multi optic and bio sleeve system, and Raman optic system for single use .....................................29 
2 Storing the bio multi optic and the resusable portion of the Raman optic system for single use ..........................29 
8Diagnostics and troubleshooting.............................................................................31 

Brief Operating Instructions Accessory optics for the Rxn-10 probe

4 Endress+Hauser

Accessory optics for the Rxn-10 probe Brief Operating Instructions

Endress+Hauser 5

1About this document

1.1 Disclaimer

These instructions are Brief Operating Instructions; they do not replace the Operating

Instructions included in the scope of supply.

1.2 Warnings

Structure of Information

Meaning

Causes (/consequences)

Consequences of non-compliance (if

applicable)

‣ Corrective action

This symbol alerts you to a dangerous situation.

Failure to avoid the dangerous situation can result in a

fatal or serious injury.

Causes (/consequences)

Consequences of non-compliance (if

applicable)

‣ Corrective action

This symbol alerts you to a dangerous situation.

Failure to avoid this situation can result in minor or

more serious injuries.

Cause/situation

Consequences of non-compliance (if

applicable)

‣ Action/note

This symbol alerts you to situations which may result

in damage to property.

Table 1. Warnings

1.3 U.S. export compliance

The policy of Endress+Hauser is in strict compliance with U.S. export control laws as

detailed in the website of the Bureau of Industry and Security at the U.S. Department of

Commerce.

Brief Operating Instructions Accessory optics for the Rxn-10 probe

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2Basic safety instructions

The safety information in this section is specific to the optics that are compatible with the

Rxn-10 Raman spectroscopic probe. Refer to the Rxn-10 Raman spectroscopic probe

Operating Instructions for additional information related to probe and laser safety.

2.1 Requirements for the personnel

•Installation, commissioning, operation, and maintenance of the probe/optics may be

carried out only by specially trained technical personnel.

•Technical personnel must be authorized by the plant operator to carry out the specified

activities.

•Technical personnel must have read and understood these Operating Instructions and

must follow the instructions contained herein.

•The facility must designate a laser safety officer who ensures staff are trained on all

Class 3B laser operating and safety procedures.

•Faults at the measuring point may only be rectified by authorized trained personnel.

Repairs not described in this document must be carried out only at the manufacturer's

site or by the service organization.

2.2 Designated use

The Rxn-10 Raman spectroscopic probe is designed for product and process development as

well as manufacturing (when used with the Raman optic system for single use). The probe is

compatible with a wide range of interchangeable, commercially available optics (immersion

and non-contact) to meet the requirements of different applications.

Recommended applications for the optics include:

Optic

Fields of application

Immersion optic (IO)

▪Development laboratory

▪Pharmaceutical: drug substance unit operations, reaction

analysis, crystallization, end-point detection, solvent swaps

▪Chemical: material identification, reaction analysis,

polymerization, cross-linking, blending

▪Food and beverage: blending, purification, natural and synthetic

components

Non-contact optic

(NCO)

▪Polymer solids (pellets, films, or powders)

▪Pharmaceutical drug product manufacturing

▪Raw material identification

▪Meat or fish quality

▪Formulation optimization

Accessory optics for the Rxn-10 probe Brief Operating Instructions

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Optic

Fields of application

bIO-Optic

▪Benchtop bioreactors to measure glucose, lactate, amino acids,

cell density, titer, and more

▪Benchtop fermenters to measure glycerol, methanol, ethanol,

sorbitol, biomass, and more

▪Use with Flow assembly CYA680 for select downstream

bioprocessing applications

Bio multi optic and

bio sleeve

▪Benchtop bioreactors to measure glucose, lactate, amino acids,

cell density, titer, and more

▪Benchtop fermenters to measure glycerol, methanol, ethanol,

sorbitol, biomass, and more

▪Use with Flow assembly CYA680 for select downstream

bioprocessing applications

Raman optic system

for single use

▪Single-use bioreactors to measure glucose, lactate, amino acids,

cell density, titer, and more

▪Single-use fermenters to measure glycerol, methanol, ethanol,

sorbitol, biomass, and more

Table 2. Fields of application

The above table lists common applications for the Rxn-10 probe and optics. There are other

possible fields of application; however, use of the device for any purpose well outside of the

fields of application described here poses a threat to the safety of people and of the entire

measuring system and invalidates any warranty.

2.3 Workplace safety

As the user, you are responsible for complying with the following safety conditions:

•Installation guidelines

•Local standards and regulations for electromagnetic compatibility

2.4 Operational safety

Before commissioning the entire measuring point:

•Verify that all connections are correct.

•Ensure that electro-optical cables are undamaged.

•Ensure fluid level is sufficient for probe/optics immersion (if applicable).

•Do not operate damaged products, and protect them against unintentional operation.

•Label damaged products as defective.

Brief Operating Instructions Accessory optics for the Rxn-10 probe

8 Endress+Hauser

During operation:

•If faults cannot be rectified, products must be taken out of service and protected against

unintentional operation.

•When working with laser devices, always follow all local laser safety protocols which

may include the use of personal protective equipment and limiting device access to

authorized users.

Service ratings may include limitations for fittings, flanges, or seals. Installer must

understand these limitations and use appropriate hardware and assembly procedures for a

pressure-tight and safe joint.

2.5 Service safety

Follow your company’s safety instructions when removing a process probe/optic from the

process interface for service. Always wear proper protective equipment when servicing the

equipment.

2.6 Important safeguards

•Do not use the optics for anything other than their intended use.

•Do not look directly into the laser beam.

•Do not point the laser at a mirrored/shiny surface or a surface that may cause diffuse

reflections. The reflected beam is as harmful as the direct beam.

•When not in use, close the shutter on the Rxn-10 probe. If an optic cap is available,

place it on the unused optic.

•Always use a laser beam block to avoid inadvertent scatter of laser radiation.

2.7 Product safety

The product is designed to meet all current safety requirements, has been tested, and

shipped from the factory in a safe operating condition. The relevant regulations and

international standards have been observed. Devices connected to an analyzer must comply

with the applicable analyzer safety standards.

Accessory optics for the Rxn-10 probe Brief Operating Instructions

Endress+Hauser 9

3Product description

The variety of optics available for the Rxn-10 Raman spectroscopic probe, powered by Kaiser

Raman technology, enables flexible sampling options in the laboratory, process

development , or single use manufacturing environment. Endress+Hauser offers immersion,

bioprocess, and non-contact optics for the analysis of liquids, slurries and solids. Optics are

available in multiple lengths and sizes to meet the requirements of different applications.

Refer to the sections below for descriptions of the optics types and their use.

•3.1: Immersion optic

•3.2: Non-contact optic

•3.3: bIO-Optic

•3.4: Bio multi optic and bio sleeve

•3.5: Raman optic system for single use

3.1 Immersion optic (KIO1)

The Endress+Hauser immersion optic is suited for use with the Rxn-10 probe in reaction

vessels, laboratory reactors, or process streams. It has a fixed focus design and no movable

parts, providing long-term measurement stability and superior signal performance. The

sealed design is the standard for use with embedded Raman Rxn analyzers.

Figure 1. Immersion optics with varying shaft length and diameter

Figure 2. Tips of immersion optics with varying diameters

A0048411

A0048727

Brief Operating Instructions Accessory optics for the Rxn-10 probe

10 Endress+Hauser

3.2 Non-contact optic (KNCO1)

Paired with the Rxn-10 probe, the Endress+Hauser non-contact optic provides contact-free

Raman measurements of samples either directly or through sight glass or translucent

packaging. These optics are ideal for use with solids or turbid media or when sample

contamination or damage to optical components is a concern.

Figure 3. Non-contact optics in varying sizes

3.3 bIO-Optic (KLBIO1)

The Endress+Hauser bIO-Optic is a versatile immersion optic used in conjunction with the

Rxn-10 probe. It measures multiple, specific bioprocessing components in real-time and is

compatible with standard PG13.5 bioreactor ports. The fixed focus design of the bIO-Optic

provides long-term measurement stability along with superior signal performance, essential

for transferable, high performance Raman-based bioprocess analysis. Available in various

industry standard lengths, the bIO-Optic is ideally suited for benchtop bioreactor/fermentor

applications requiring headplate entry. The 120 mm version is compatible with

Endress+Hauser flow assembly CYA680.

A0048410

A0048676

Ce manuel convient aux modèles suivants

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