Eicom AZ-X-Y Manuel utilisateur
US: www.eicomusa.com - info@
eicomusa.
com | EU: www.eicomeurope.com - info@
eicomeurope.
com
User’s ManUal
Concentric Microdialysis Probe
Model AZ-X-Y
• AllEicomproductsareforanimalresearchpurposeonly.
• Keeptheprobeawayfromphysicalshock.
• Inletneedleislongerthanoutletneedle.
• Duramustbebrokenpriortoinsertionintothebrain.
AZ-X-Y Probe
the probe membrane. After flushing for several minutes (it is not
necessarytoflushforanextendedperiodoftime)themembranemay
“sweat”orappearwetthisisnormal,notduetodamageoraclog.
2. Ifnoflowisobservedviatheoutletneedle,soaktheprobeindistilled
water for a few minutes and repeat instructions in item 1. After
conrmingthedistilledwaterflowfromtheoutlet,pleaseremovethe
probefromplasticsheathandconrmnoleaking.
3. We test each probe for defect/leaking prior to shipping. If you nd
adefectiveprobeafterfollowingtheabovestepsEicomwillreplace
theprobeatnocharge.Wedonotguaranteeprobesafterinsertion
toanimals.
Removing Air Bubble
1. Ifthereisanairbubbleintheprobe,pleaseremoveit.Becausethe
airbubblewillcovertheactivemembranesiteandkeeptheperfusate
away.Thiswillresultinalowerrecoveryrate.Theairbubbleshouldbe
removedbeforestudy.Ifitissmall,itwilldisappearincaseofinvivo
studybutmaynotinvitro.
2. Perfusateneedstobedegassedbeforeuse.
3. Toremovetheairbubble,pleaseintroduceabigairtotheinletand
flushout.Donotconnectoutlettubingatthistime.Thebigairbubble
willtrapandremoveasmallairbubble.
Connecting Outlet
1. Conrmneitherleakingorairbubblesarepresentinthemembrane.
Settheflowrateonthesyringepump.Astandardflowrateis1-2μl/
min.Ahighflowratecoupledwithalongoutlettubingcanresultin
damageoftheprobemembrane.
2. Connect Teflon tubing (model JT-10) or other tubing to the outlet.
A long tubing length (more than 150 cm) can cause leaking of the
membraneortheliquidswivelduetotheinducedhighpressure.Ifyou
usetubinglongerthan150cm,pleaseavoidusingflowrateshigher
than 2 μl/min. Polyethylene tubing can cause confounding peaks
whenanalyzingsamplesusingHPLCwithelectrochemicaldetection.
Cleaning Probes and Storage
Removetheprobefromthesystemandflushwithdistilledwatertowash
out salts. Then put the probe in the distilled water and place at 4°C for
storage. Keep the membrane wet to prevent itfrom shrinking. Keep the
probeawayfromphysicalshock.
Cleaning Lines (Teflon, FEP tubing)
Bacteriagenerationandproteinpollutionoftubingcanbeacauseoflow
recoveryrates,especiallyintheoutlettubing.Inordertoavoidthisproblem,
washthelineswith50timesdilutedcommercialkitchenbleach(chlorine)in
purewater.Perfusethesolutionintothelines(nottheprobe)andleaveitfor
atleastonehour,thenthoroughlyrinsewithwater.
Perfusion Solution
Wearecommendusinganon-bufferedaCSFsolutionbecausephosphate
buffersolutionsmayprecipitatecalciumphosphateandcanresultinclogs.
Probe Check
1. Injectdistilled water using a1 or 2 mldisposable (plastic) syringe.
Connect the syringe to the inlet (longer needle) of the probe using
Bitontubing(JB-30).Theprobemaybecomecloggedordamagedif
youinitiallyflushwithasaltsolution.Werecommendusingdistilled
waterfortheinitialprobeflush.Donotconnecttubingtotheoutlet
oftheprobeduringflushing.Pleaseconrmtherearenoleaksfrom
AD12-0718
inner 0.105/0.040 mm PFS
outer 0.63/0.33 mm SUS
inner 0.150/0.075 mm PFS
outer 0.63/0.33 mm SUS
0.3/0.2 mm SUS
0.18/0.12 mm SUS
Inner Dead Volume
inlet: 0.05 µL
outlet: 0.4 µL
9.0 mm
12.0 mm
X + 5
PFS: Polyimide-Resin
Coated Fused Silica
Membrane: Artificial Cellulose
Cut off: MW 50,000
OD/ID: 0.22/0.20 mm
SUS (Stainless Steel) 316
Y
Model: AZ-X-Y
X = (guide cannula length)
Y = (membrane length)
US: www.eicomusa.com - info@
eicomusa.
com | EU: www.eicomeurope.com - info@
eicomeurope.
com
Cap Nut
(AC-1)
Guide Cannula
(AG-X)
Stero Adapter
(SAG-X)
Clamp of
Stereotaxic Apparatus
not provided from Eicom
Guide Cannula crown is
acrylic resin made and
adhesive to acrylic
dental cement but Cap
Nut is not acrylic resin.
Stero Adapter
(SAG-X)
Dental Cement
Ancor Screw (AN-3)
After confirming fixation of the guide
cannula, loose the cap nut and remove stero
adaptor from the guide cannula. Instead of
the stero adaptor, insert the dummy cannula
(AD-X) and fix with a cap nut.
Dummy Cannula (AD-X)
CBA
ED
CongurationofStereoAdaptor(SAG-X),GuideCannula(AG-X),DummyCannula(AD-X)&CapNut(AC-1)
USA
10060CarrollCanyonRd.,Ste100,SanDiego,CA92131
TollFree(888)680-7775Fax(858)560-8040
Europe
8TerenurePlace,Terenure,Dublin,D6WY006,Ireland
Phone+35319022700Fax+35314430784
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