Biotage®Flash 75/150 User Manual | © Biotage 2018
Safety
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The system must be placed in a well-ventilated fume hood
or an equivalent enclosure that is capable of exhausting
2.5 m3/min (90 ft3/min). Follow local and national safety
regulations for installing a system inside a fume hood and the
safety regulations supplied by the fume hood manufacturer.
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Never install or use the system near
a potential ignition source.
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The system, when operated with non-polar solvents
such as hexane, methylene chloride, etc., can build up a
high static electricity charge, which in certain conditions
can be dangerous. To eliminate any risk of a static
discharge, the systems and ancillary containers must
be grounded before use as described on page 12.
Failure to follow these grounding instructions may result
in equipment damage, personal injury, or death.
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A trained person must verify that the system and
ancillary containers are grounded before each run.
Nominal resistance must be below 5 Ohm between
ground and each metal point in the system.
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Always connect inert gas such as nitrogen to
the gas inlet on the AM-190 manifold.
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Ensure that the inert gas tubing connected to the
system cannot come in contact with chemicals.
Corrosives and solvents can dissolve the tubing.
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The AM-190 manifold must be mounted horizontally
so that the water trap can function correctly.
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The outlets of the three safety relief valves (located on the
radial compression module, AM-190 manifold, and solvent
reservoir) must be directed away from the operator.
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Ensure that the tubes are not longer than
necessary for your setup so that there is no
risk of stepping or tripping over them.
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To avoid injury to yourself or damage to the
system, set the inert gas supply pressure to
between 100 and 125 psi (6.9 to 8.6 bar).
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Do not over-tighten the fittings or the
tubing may become damaged.
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Never modify the system components in any way
and always use spare parts supplied by Biotage.
Operation
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Use the system only for its intended purpose, as described
in the user documentation delivered with the system and
user documentation available at www.biotage.com. If the
system is used in a manner not specified by Biotage, the
safety features of the system may be compromised.
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The system must not be operated unattended.
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Never operate the system if damaged.
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The system is operated using pressurized inert
gas. Ensure that all seals are properly mounted
and that all clamps are properly closed before
introducing pressurized gas into the system.
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Confirm that the upper V-band clamp is tight before
compressing the radial compression module. Only
hand-tighten the clamp, to a maximum torque of 11.3 Nm
(100 lbf-in). If the cartridge is not sealed properly, replace
the O-ring and retry, or contact Biotage 1-Point Support.
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Inspect the V-band clamps before and after each use.
The clamp must be replaced when it shows any signs of
wear and tear, or at 500 pressure cycles, whichever comes
first. Only use clamps and screws supplied by Biotage.
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Never loosen the V-band clamps when the radial
compression module is under pressure. A red
pressure indicator on the radial compression
module sticks out when the pressure is above
20 psi (1.4 bar); see Figure 2 on page 2.
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Never remove a tube or system component
when it is under pressure.
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Reduce the pressure using the solvent pressure
regulator (labeled PRR-02)to less than 50 psi
(3.5 bar) before blowing down the Flash 150
radial compression module with inert gas.
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When closing the solvent reservoir lid, ensure that
it is seated properly to ensure a secure seal.
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Never run the system without a cartridge,
or with the wrong size of cartridge.
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Always equilibrate the cartridge.
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Equilibrate the cartridge at a low flow rate
to avoid extreme temperature rise.
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Only use waste/fraction collection vessels
that are grounded or made of glass.
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If the system is placed in a walk-in fume
hood, a fume extractor must be used for open
waste/fraction collection vessels.
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Follow all applicable safety procedures
when working with bottled gas.
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Follow all generally-accepted lab safety procedures
and applicable laws and regulations.
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Always follow local and national safety
regulations related to storage, handling and
disposal of chemicals, samples and waste.
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Read and understand the safety data sheet (SDS)
provided by the chemical manufacturer before
storing, handling, working with, or disposing
of any chemical or hazardous substance.
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Personnel working with or near the system must
wear applicable safety clothing and gear (such as
solvent-resistant clothing and gloves, steel toe
shoes, and face and eye protection) that comply
with local and national safety regulations.
