Biogal PCRun Manuel utilisateur
Table of Contents
Contents of Box
Starting the PCRun Reader
Assembly of PCRun Reader
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Choosing the Default Test Protocol
Running a PCRun Reaction
General Feature
Safety Precautions
User Responsibility
Analysis of Results
Reset PCRun Reader
Aborting a Run
Downloading Files from the PCRun Reader
Deleting Files from the PCRun Reader
Analyzing PCRun Graphs
Instructions for Use of the PCRun Reader Simulator
5
8
6
9
10
7
13
14
15
16
19
Unpacking and Assembling
Operation Guide
Page
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4
5
Unpacking and Assembling
Fig. 4: USB /Micro-USB
connector
Fig. 2: Power cable
Fig. 7: TransformerFig. 5: Stylus
Fig. 1: Reader
Fig. 3: On/off switch
Fig. 6: USB Portable
Memory Stick
(present only in certain
models)
Contents of Box
6
Attention!
Before installing the PCRun Reader please pay attention to the following:
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Do not place the Reader in an area which receives direct light (natural or articial). During
the amplication process light is emitted from the reagents. The generated light is collected,
interpreted and transformed to a readable signal of positivity or negativity. Strong external
light may cause background noise which may be translated into a false positive signal.
Do not place the Reader on the same table with apparatus that my cause vibrations
(centrifuge, vortex, shakers etc). Vibrations will result in unstable readings.
Remove the PCRun Reader (Fig. 8) from the protective packaging and rest on a solid, level
surface allowing approximately 25 cm of clearance space above the unit for opening. Allow
5 cm distance from any wall or object to allow for proper ventilation. Make sure that the
Reader is not housed in area with strong light.
Assembly of PCRun Reader
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Fig. 8: Front view
Lid
LCD touchscreen
Indicator light
Stylus
7
Fig. 9: Back view
On-off switch
Portable memory
port
Mini USB port Transformer
Outlet
Connect the transformer (Fig. 7) to the power cable (Fig. 2) and then to the back of the PCRun
Reader (Fig. 9). If the Reader comes with an external on/ off switch (Fig. 3) connect it to both the
back of the PCRun Reader and to the transformer cable. The transformer will adapt the voltage
to 220 or 110.
The PCRun Reader is a complete system which contains a heater and luminometer to be used in
conjunction with PCRun reagents designed for the amplication process and analysis of PCRun
molecular reactions. The unit contains a graphical LCD touch panel on which the nal results are
displayed.
• Users are responsible for familiarizing themselves with the product instructions and
limitations.
• External factors such as sample quality and preparation, as well as testing protocols and
laboratory techniques, may inuence the nal results. It is the user’s responsibility to select
the proper sample material which meets the criteria of the chosen test.
• As with any test method utilized for in vitro diagnostics, the results obtained from use of the
PCRun technology should be interpreted together with the results of other tests.
• Operate the instrument on a solid dry surface away from a strong light source.
• Ensure the laboratory electrical supply is appropriate and surge protected.
• The PCRun Reader should be switched off when not in use.
• Do not open the casing of the Reader.
• Maintenance of the unit must be carried out by Biogal personnel only.
General Feature
User Responsibility
Safety Precautions
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8
Fig. 10 | Model B
Power cord
connection to
transformer
Connection to
Reader
Fig. 10 | Model A
Prior to using the PCRun Reader make sure, that it has been set up according to the
“Unpacking and Assembling” instructions.
1.1
Turn on the PCRun Reader using the on/off switch located on the power cord
(Fig. 10, Model A) or on the back of the Reader (Fig.10, Model B).
Operation Guide
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1. Starting the PCRun Reader
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1.2
The following display will appear briey (Fig 11). The
PCRun Code # and the rmware that is installed in
the Reader are located on the bottom of the screen.
1.3
The main screen (Fig. 12) will replace the Logo
display. The following details will be displayed:
2.1
The default test protocol setting is GENERIC and
is suitable for most of the PCRun reactions.
Alternate protocols may be recommended for
certain kits. Prior to using the Reader, check the kit
instructions to determine which protocol is suitable
for your test. In the case that a different protocol is
recommended, the following instructions will explain
how to change the program temporarily or to dene
a new default program. Using the stylus, activate
the Test: GENERIC tab (Fig. 12). The names of the
various protocols will appear in the window. Choose
the recommended protocol (Fig. 13).
• Warm up ?
• Test: GENERIC
• Settings
• Files
• Present date and hour
• Thermometer with ambient temperature
2. Choosing the Default Test Protocol
Fig. 11
Fig. 12
Fig. 13
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10
3.1
Using the stylus press Warm Up? (Fig. 12). The
message on the screen will change to grey and
the words Warming up will appear. In addition, the
indicator light and the thermometer will change to
orange (Fig. 14).
If you will be using the protocol for a single application press Select located on the bottom right
hand side of the screen. If you choose to make the new protocol the default setting, press Make
default located on the bottom left side of the screen. Follow by pressing Select.
3. Running a PCRun Reaction
3.2
Once the temperature of the Reader has reached
its target (60°C) the message will change to Run test.
The indicator light and the thermometer on the touch
screen will change to green. The temperature noted
above the thermometer will be 60°C (Fig. 15).
Fig. 15
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3.3
Open the lid of the Reader and load the reaction tubes
into the internal wells. Take note of where the tubes
are placed (Fig. 16).
Fig. 16
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