Abaxis VetScan HM5 Manuel utilisateur

QUICK REFERENCE GUIDE

Easy operation — fast results

Closer to Care.

• Unpack the VetScan HM5 analyzer from its shipping container and put out the

accesory box.

Note: Store the shipping box, accessory box, and packaging

materials in case you need to use them later.

• Place the analyzer on a level surface.

• Use the latch to open the side door on the unit, as shown.

• Inspect the unit for any visible signs of damage.

• Remove foam 1 (and tape2) as shown below.

Unpacking and Setup

< 45 cm not preferred

Up to 45 cm

below is ok

Ideal

•Open the accessory box and use this list to make sure you received all

components of the Vet-Scan HM5 system.

• VetScan HM5 Analyzer.

• VetScan HM5 Operator’s Manual.

• External power supply.

•Mini-keyboard

• Four sample tube adaptors.

• Reagent Tubing Kit.

Selecting a Location

The analyzer must be installed in a suitable location.

• The location should be ﬂat, level, sturdy, vibration-free, and as dust-free as possible.

• Leave at least 50 cm of clearance on all sides of the instrument to allow for

adequate airﬂow and access to connectors.

• Maintain a minimum of 20 cm behind the analyzer’s rear panel for heat dissipation

and tube clearance.

• Leave enough space for the reagent pack.

Connecting the Reagent Pack

• Place the reagent pack near the analyzer, either beside

or behind the instrument.

• For best results, place the reagent pack at the same level as

the analyzer. If you have to place the reagent pack on a lower

level, make sure that it is no more than 45 cm below the

level of the analyzer’s reagent inlets, as measured from the

bottom of the reagent pack to the bottom of the analyzer.

Note: Do not place the reagent pack on a higher level.

Closer to Care.

Connect the reagent pack as follows.

• Open the plastic bag located in the accessory box, and take out the six

color-coded reagent tubes and caps with drop-down tubes.

• Attach the supplied connector

tubing kit to the instrument,

as shown.

• Open the reagent pack box. Open each reagent container (lyse, lyse 2, rinse, cleaner,

and diluent) by unscrewing the container caps and piercing the bottle top seals.

• Re-cap each container using the bottle cap with the appropriate color-coded connec-

tor and drop-down tube.

•Flip diluent canister 180 degrees prevent reagent errors by ensuring that tubes are

not kinked when the box lid is closed.

•Connect each color tube to its matching color outlet. The connectors should

snap into each other when properly connected.

• Connect waste bottle.

Initialization of the HM5

• Connect Power Supply.

• Install paper into the built in printer.

• Turn on the analyzer by using the power switch on the upper le of its rear panel.

•The analyzer then displays several screens while it runs its soware start-up procedure.

•Touch Measure, then touch Run.

•When prompted, touch OK to run a blank. ( Also see “Running a blank”).

•The analyzer then initializes its pneumatic system and runs a blank measurement.

Attach the 2” tubing to the

stainless steel hose barbs

Attach the male

connector to the

reagent tubing

Snap the male connector into the

female connector

Attach the 2” tubing to the

stainless steel hose barbs

Attach the male

connector to the

reagent tubing

Snap the male connector into the

female connector

• Insert the six male connectors into

the free ends of the color-coded

reagent tubes.

Test: analyze samples and

run blanks

HOMESCREEN

Database: search, retrieve, view

and manage up to 5,000 records

Settings: manage printer,

testing, export and date/time

and other analyzer settings

Printer: Manage printer settings

and printout options

Maintenance: clean, calibrate,

run quality controls and manage

reagent status

Exit: select shutdown options and

manage user accounts

General Settings: select

analyzer options and language

Date and Time: change date

and time options

Measurement: select units, set

normal ranges for species and

data export options

Touchscreen Icons (list of icons and their meanings)

CBC analysis menu

stores all results

from test menu

adjust settings for

analyzer controls,

printing, and data export

maintenance,

cleaning, QC

and calibration

shutdown and

logout options

Closer to Care.

• Touch the Measure icon.

• Enter the Patient ID on the “New Sample Screen”.

•Select animal Type.

•Mix the sample well by slowly inverting the tube 10-15 times. Insert the

well-mixed EDTA preserved whole blood sample into the appropriate tube

adapter, on the sample rotor, and remove the cap.

• Touch the Run button.

If your HM5 is set for autoprint, your results will be

printed automatically.

If not, press the Print button to print results

Test Results (and blank cycles) are automatically saved

to the HM5 database.

They can be viewed by pressing the database function key

Running a Sample

Analysis is complete in less than four minutes. All results and histograms

are automatically saved in the analyzer’s database.

• When analysis is complete, the analyzer displays all measured and

calculated parameters, as well as the WBC, EOS, RBC, and PLT

histograms:

If the analyzer is not set to print results automatically, press Print to

print the results.

Results falling outside of the speciﬁed species reference range are

marked as follows:

• Results above the reference range are ﬂagged and marked with a

plus sign (+).

• Results below the reference range are ﬂagged and marked with a

minus sign (–).

• Warning and error ﬂags are marked with upper- and lower-case letters

at the bottom.

Interpreting Results

Closer to Care.

Running a Blank

A blank must be run every 12 hours. Each blank measurement remains

valid for 12 hours of continuous operation, aer which the analyzer displays

BLANK needed and a new blank is required. In addition, a new blank must

be run each time the analyzer has been powered on and oﬀ again.

• If the analyzer is in standby mode, touch the measure icon.

• Touch the Run button.

• If a blank is necessary, the “Blank needed” screen appears.

• Touch OK to conﬁrm and run the blank.

• Check the results to see whether the blank falls within the acceptable

ranges shown in the following table. If so, press Accept.

Acceptable blanks will have

the following readouts:

WBC 0.0-0.5

RBC 0.0-0.05

PLT 0-26

HGB 0-10

Running Controls

Controls are run to assess if the analyzer is maintaining calibration.

• Touch the Maintenance icon.

• Touch the Quality control button.

• Touch the QC level (e.g. QC Normal) that will

be used.

• Touch References to load the assay and gap values

• Enter QC values (choose one of three methods):

a. Enter manually - values can be entered by

hand by touching the appropriate ﬁelds.

b. Enter by USB pen drive - the QC values ﬁle

can be loaded by inserting the USB drive into

a USB port and touching Load.

c. Enter by Barcode reader - press Load

Datamatrix on the Quality Control screen.

Scan the 2D barcode on the QC package insert.

• View table of QC databases.

• View QC diagram (for a Levy-Jennings graphic

representation of the QC history).

• If the QC results were within range, proceed

to running samples. If the QC was out of

range, repeat QC run. If the QC is repeatedly

out of range, proceed to calibration.

(see Calibrating the Analyzer).

Closer to Care.

Changing the Reagent Pack

• In the Maintenance menu, select Reagent status.

Select Replace and then Change pack.

• Select Prime All to prime the reagent tubing.

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