AB Sciex API 3200 System Instructions d'installation
Hardware Guide
API 3200™System
D1000092204 B
May 2010
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Document Number: D1000092204 B 3
Contents
Chapter 1 System Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Principles of the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Applications for the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Features of the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
How the Instrument Works. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Parts of the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Power Controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Sample Introduction Features and Status Indicators . . . . . . . . . . . . 8
I/O Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Gas Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Source Exhaust System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Vacuum Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Shutting Down and Turning on the System . . . . . . . . . . . . . . . . . . . 12
Instrument Safe Fluids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Source/Gas Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Turbo V™ Ion Source Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Compound Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Technical Support. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Chapter 2 3200 Series Parameters . . . . . . . . . . . . . . . . . . . . . . . . . 17
Contents
4 Document Number: D1000092204 B
Document Number: D1000092204 B 5
1
System Overview
The API 3200™ LC/MS/MS system includes a triple quadrupole mass
spectrometer, a Turbo V™ ion source, a computer, and the Analyst®software.
Figure 1-1 API 3200™ instrument and bench
WARNING! If you need to move the system, contact an FSE to assist you. Risk of
personal injury or instrument damage.
Note: Before you operate the instrument, make sure you have read the Safety Practices
guide.
Line Adjustment
Transformer
Roughing Pump
Bench
Mass Spectrometer
Front View
Ion Source
System Overview
6 Document Number: D1000092204 B
Principles of the System
This section describes features and applications of the API 3200™ LC/MS/MS
system.
Applications for the System
The API 3200 system is designed for quantitative analysis of small molecules.
This application involves measurement of specific molecular weight compounds,
usually a drug or metabolite in a liquid sample, and their resulting fragment ions
for determining the exact quantity of the compound in the sample of interest.
Quantitation is performed using a standard curve of mass spectrometer signal
intensity for various known concentrations of the compound. The signal in the
test sample is then compared to the standard curve to determine the
concentration.
The typical scan type used for this application is MRM (Multiple Reaction
Monitoring).
Features of the Instrument
The API 3200 system is a benchtop triple quadrupole mass spectrometer for
sensitive quantitative analyses of biomolecules found in a wide variety of
biological matrices. It has the following features:
•The standard Turbo V™ ion source provides a plug-and-play
TurboIonSpray®probe and APCI probe, a durable ceramic interface, and
compatibility with high flow rates.
•LINAC®collision cell eliminates cross-talk and allows for multi-component
analysis during an LC run.
•Rugged Curtain Gas™ interface technology combined with improved gas
dynamics reduces maintenance and increases uptime.
•Scanning in a mass range of m/z 5–1800.
•Integrated switching valve and integrated syringe pump provide
convenient sample introduction flexibility.
•Additional compatible sources, including the DuoSpray™ ion source,
PhotoSpray®ion source, and NanoSpray®III ion source components,
provide further flexibility.
•IDA (Information Dependent Acquisition) maximizes the amount of
information that can be acquired in an LC scan.
•Compatible with additional software packages, such as Metabolite ID
software, Automaton software, BioAnalyst™ software, and more.
How the Instrument Works
The API 3200 mass spectrometer has a series of quadrupole filters that transmit
ions according to their m/z (mass-to-charge ratios). Ions entering the ion path are
first focused by Q0 before passing into Q1.
Q1 is a filtering quadrupole that sorts the ions before they enter Q2, a LINAC
collision cell in which ions can be broken into fragments by collisions with gas
molecules. This technique allows for the design of experiments that measure the
m/z of product ions to determine the composition of the parent ions. After
Hardware Guide
Document Number: D1000092204 B 7
passing through Q2 the ions enter Q3 for additional filtering, and then enter the
detector. In the detector, the ions create a current that is converted into a voltage
pulse. The voltage pulses leaving the detector are directly proportional to the
quantity of ions entering the detector. The instrument monitors these voltage
pulses and converts the information into a signal. The signal represents the ion
intensity for a particular m/z and the instrument displays this formation as a mass
spectrum. For more information, see the following figure, Figure 1-2 Ion optics
path.
Figure 1-2 Ion optics path
The API 3200 system uses the Turbo V ion source to produce ions from liquid
samples. The Turbo V source can use either the TurboIonSpray probe or the APCI
(atmospheric pressure chemical ionization) probe. The instrument is configured to
perform complex MS/MS analysis, but for less rigorous analytical requirements it
can perform single MS scans.
Parts of the Instrument
This section contains diagrams of the instrument and general descriptions of the
main components.
Figure 1-3 Front view
Curtain Plate
DP (Orifice)
Skimmer
IQ1 IQ2 LINAC
®
Collision
Cell
IQ3
ST
DF (Deflector)
Detector
CUR (Curtain Gas™ flow)
EP (Q0) RO1 (Q1) RO2 (Q2) RO3 (Q3)
Front View
Mass Spectrometer
Ion Source
Integrated
Switching
Valve
Integrated
Syringe
Pump
System Overview
8 Document Number: D1000092204 B
Figure 1-4 Back view
Power Controls
The API 3200™ instrument power controls are located near the back left corner
of the instrument, wherein the power distribution module is enclosed. This area
contains the main power switch and the external AC power supply outlet.
Figure 1-5 Power switch and supply
Sample Introduction Features and Status Indicators
This section describes the features and controls housed on the front left area of
the instrument, adjacent to the source housing.
SHEATH
GAS
MAX 105 PSIG
CAD
GAS
MAX 60 PSIG
VALVE
WASTE OUT
EXHAUST
WASTE OUT
CURTAINGAS
SUPPLY
Max 60 PSIG
GAS 1 / GAS 2
Max 105 PSIG
EXHAUST SUPPLY
MIN 55 PSIG MAX 60 PSIG
SERIAL
SOURCES
CAD GAS
BACKING PUMP
AUX I/O IEEE-4888
Rear View
Ion Source
Mass Spectrometer
I/O Panel
Gas Panel
Roughing
Pump
Connection
AC Power
Supply
CAD Gas
Adjustment
Valve
Main Power
Switch
207-242V
50-60 Hz 10A
External
A/C Power Supply
Hardware Guide
Document Number: D1000092204 B 9
The Instrument Status lights indicate the status of the instrument vacuum. When
the operational vacuum conditions are satisfied and the instrument is in analysis
mode, the Ready light (green) is illuminated and the Fault light (red) is
extinguished. The Fault light flashes when a vacuum fault is detected. In Pump-
Down mode, the Ready light flashes for the duration of the sequence.
Directly below the source housing is a set of conveniently integrated sample
introduction features. An integrated switching valve with associated Load and
Inject buttons (marked A and B, respectively) can be used as a manual injector
when optimizing for an analyte using FIA, or for diverting LC flow when any
precipitate or salts initially come off the LC column. Below that, an integrated
syringe pump is available for infusing standards when calibrating the instrument
or optimizing for an analyte.
Figure 1-6 Sample introduction features and status indicators
I/O Panel
The input/output panel is located at the center back of the chassis. This panel
contains several connection ports for the system:
•The IEEE-488 (GPIB) port provides a connection to the system’s computer.
LOAD INJECT
AB
Integrated
Switching
Valve
Integrated
Syringe
Pump
INSTRUMENT STAUS
INSTRUMENT STATUS
FAULT
FAULT
READY
READY
LOAD INJECT
AB
System Overview
10 Document Number: D1000092204 B
•The sources port facilitates communication to optional ion sources, such
as the control valve for the DuoSpray™ ion source, or provision of 24 VDC
for the PhotoSpray®ion source.
•The AUX I/O outlet allows for communication with necessary peripheral
devices, such as autosamplers or UV detectors.
•A serial port provides a connection point for additional device
communications.
Figure 1-7 I/O panel
Gas Panel
The gas panel houses the gas supply connections and the external connections
for the source exhaust system.
I/O Panel
SERIAL
SOURCES AUX I/O IEEE-488
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