Wealtec CB-1 Manuel utilisateur
Block Cooler System
(CB-1)
Installation and Operation Manual
Version 1.0
Item# 03060
*This instrument is intended for laboratory use only
Block Cooler V1.0
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Index
A. Important Notice ---------------------------------------------------------------- 1
A-1. Warranty
A-2. Technical and Service Contact
A-3. Safety Notice
A-3-1. Certification
A-3-2. Safety Information
B. Introduction ---------------------------------------------------------------------- 3
B-1. Specifications
B-2. Product Description
B-2-1. Block Cooler System Hardware Overview
C. Installation of Block Cooler System (CB-1) ----------------------------- 9
C-1. Package List
C-2. Installation of Block Cooler System
C-2-1. Installation Procedure
D. Operation ------------------------------------------------------------------------ 11
E. OFFSET Function -------------------------------------------------------------- 12
F. Troubleshooting ---------------------------------------------------------------- 14
F-1. General symptom
F-2. Installation of Block Cooler System
G. Care and Maintenance -------------------------------------------------------- 16
H. Order Information -------------------------------------------------------------- 17
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A. Important Notice
Before setting up and operating the instruments of Block Cooler (CB-1) system, please
carefully read these instructions to get familiarized with the installation and operation process.
Instructions should be read by experienced individuals before operating the instruments.
Any improper usage of the instruments may cause damage. Please refer to the safety
notice included with this equipment.
The instruments shall not be modified or altered in any way. Any modification or alteration
will void the warranty, void the regulatory certifications and create potential safety hazard.
Wealtec is not responsible for any injury or damage caused by using the instruments for any
non-intended purpose or injury as a result of modification of the instruments by any person who
is not authorized by Wealtec Corp.
A-1. Warranty
Block Cooler system is warranted to be free from defects in materials or workmanship for a
period of one year from the original invoice date, under normal usage. Any defects occurring
during warranty period, Wealtec Corp. will repair or replace defective products or parts without
charge unless the defects arise from conditions outlined below. The defects described below are
specially excluded from Wealtec warranty policy.
1. Improper operation of the instrument.
2. Repair or modification by any person who is not authorized by Wealtec Corp.
3. Damage caused by any (in)-direct accident, neglect or misuse.
4. Damage caused by disaster.
5. Damage caused by any improper solvents or samples
A-2. Technical and Service Contact
Most of the operation details are described in this instruction manual to assist and guide
operator for an appropriate solution. For any other technical/ service questions, please contact
your local representative or contact Wealtec international technical/ service specialist by E-mail:
Block Cooler V1.0
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A-3. Safety Notice
A-3-1. Certification
The block cooler system is designed to meet the international electrical safety standards
EN61010-1 and EMC regulations. This product designed to meet CE requirements and if
operated according to the guidance of the instruction manual, is certified safe. Any modification
or alteration will void the warranty, void the regulatory certifications and create potential safety
hazards.
A-3-2. Safety Information
¾Do not apply more or lower than the rated voltage specified on the instrument or it may
cause damages to the instrument.
¾Do not splash water or liquid onto the block cooler system, the spilled water or liquid
may cause malfunction to the block cooler system.
¾Do not touch or remove the 48-Well aluminum block directly with hand while heating,
cooling or when the block is still hot or cold.
¾Do not directly pour water/liquid/ice on the high or low temperature heating block to
cool down or stabilize the temperature of the block to the room temperature. It may
cause damage to the instrument and fast-evaporated water/liquid may be harmful to
the operator.
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B. Introduction
Block Cooler System (CB-1) is a standard cooling unit for laboratory use. The compact
cooling unit temperature ranging 0 – 75℃allows customizing settings for specific temperatures
or utilizing the nine frequently used pre-set temperatures for various molecular biology or
chemistry applications. The universal 48-well aluminum block is a single block designed to suit
various types of microtubes without the need of the multiple blocks. An applicable samples for
block cooler accordingly for various temperatures is given in the table below:
Selectable Temperature
Range Recommended Applications
0 °C Replace the ice buckets for samples & enzymes storage;
4 °C
Sample cooling & storage during operation –
i.e: Store nucleic acid at 4°C in long PCR at final stage, DNA
purification kit rinsing and sample with 70% ethanol at 4°C,
hybridization process when adding polyAto hold at 4°C in nucleic
acid.
cDNA synthesis experiment –
i.e: Placing oligonucletides or polymerase at 4°C.
Pre-thaw the primary antibody for continuous uses –
i.e: western blotting procedure.
Enzyme reaction –
i.e: ligation steps of gene construction.
Cell lysis experiment –
i.e: Resuspend the protein in lysis buffer at 4°C.
General immunoprecipitation procedure.
16 °C Enzyme reaction –
i.e: Restriction enzyme activity for DNAligation.
25 °C
Biological response assay.
Enzyme catalysis activity reaction.
In long PCR reaction at final stage “hold”.
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37 °C
In cell culture procedure to dissociate the cell with
Typsine/DNAase I solution in HBSS medium or resuspend with
DNase I solution only in DMEM medium.
The denature DNAsteps in DNA extraction kit.
DNA/RNA probe preparing.
The incubation steps in cDNA synthesis.
Bacteria culture incubation or purification.
Enzyme reaction –
i.e: Restriction enzyme reaction and RNAase reaction by DNA
extraction or Flow cytometry ofApoptosis.
Incubate the plug at 37°C in Pulsed Field Gel Electrophoresis.
For gel digestion pre-treatment before MALDI-TOF MS analysis.
42 °C
The reverse transcription temperature by RT-PCR.
Enzyme reaction –
i.e: DNA ligase gene reaction for thermosensitive mutant
application.
The incubation procedure of phage purification.
Gene transformation incubation –
i.e: Transfer gene to E. Coli strain for expression.
55 °C
The steps required on cDNA synthesis.
Chemical reaction –
i.e: The gentle heating steps by DNAextraction kit solution.
Enzyme reaction –
i.e: RNAase reaction.
Gel digestion –
i.e: The oligonucleotides purify from SDS-PAGE gel.
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65 °C
In RT-PCR experiment, continuous to hydrolysis steps.
Inactivate enzyme reaction –
i.e: Inactivate the proteinase K reaction in DNA/RNAprobe
preparing.
The incubation temperature in Bacteriophage DNApurification
procedure.
Keep the melted Agar / Agarose medium warm to extract nucleic
acid.
72 °C
Chemical / enzyme reactions –
i.e: Inactivate enzyme reaction.
Sample dissolving in solution –
i.e: Agarose dissolving.
Denatured sample –
i.e: Pre-heating protein molecules before SDS-PAGE running.
Customize Temperature
Between 0-75 °C
Specific incubation temperatures for chemistry / molecular biology
experiment requirements.
Sample & chemical dissolving.
B-1. Specifications
Temperature range 0 – 75℃
Number of pre-set
temperature 9 Points (0, 4, 16, 25, 37, 42, 55, 65 and 72℃)
Temperature accuracy +/- 0.1℃
(Full temperature range except at 25℃)
Cooling performance 35 minutes (From 25℃to 0℃)
Temperature controller Microprocessor with PID algorithm
Temperature
control mode Digital
Temperature calibration Built-in
Display type LCD display
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Heating /
Cooling element Peltier
Heating power (Watt) 75 max.
Over temperature cutoff 100℃
Power 90–264 VAC, 47–63 Hz
Dimension (L x W x H) 200x304x184 mm (7.87”x11.97”x7.24”)
Weight
(With block) Net~6.5kg (14.3 pounds)
Gross~7.5kg (16.5 pounds)
Block capacity 1
Operating conditions Temperature : 0 – 26 ℃
Humidity : 10% to 90% R.H. Non-condensing
Aluminum block
Block type 48-Well aluminum block
Well type 8 x 0.2 ml 21 x 0.5 ml 15 x 1.5 ml 4 x 2.0 ml
Number of holes 8 21 15 4
Tube type 0.2 ml
microtube 0.5 ml
microtube 1.5 ml
microtube 2.0 ml
microtube
Hole diameter (mm) 6.5 8 11 13
Hole depth (mm) 20 29 34.5 34.5
Block material Solid anodized aluminum
Dimension (L x W x H) 107x71x38 mm (4.22”x2.8”x1.5”)
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B-2. Product Description
B-2-1. Block Cooler System Hardware Overview
Control panel
¾“RUN/STOP” key – Press this button in order to run or stop the block cooler.
¾“OFFSET” key – Press this button and adjust the offset value in order to alter the
temperature of the block cooler system to the desired temperature as per to users
Process indicato
r
Power switch
Protection lid
Protection lid handle
48-Well aluminum block “Run/Stop” key
“Offset” key
Ready indicator
Heating indicator
Power indicato
r
Coolin
g
indicato
r
LCD display
Up or down key
for selected parameter
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calibrated thermometer.
¾Power indicator will turn on if the block cooler system is turned on.
¾“ ” – Cooling indicator will turn on if the system is desired to cool down.
¾“ ” – Heating indicator will turn on if the system is desired to heat up. In the other
word when the set temperature equals to the actual block temperature.
¾“ ” – Ready indicator will turn on if the system has reached the desired
temperature. In the other word when the set temperature equals to the actual block
temperature.
¾“ ” – Process indicator will turn on while the system is progressing to the desired
temperature.
¾“ ” & “ ” – Up and down key is used to adjust the desired temperature value
and the desired mode of the temperature.
¾LCD display – Displays the temperature value and the mode settings. LCD display
allows for easy system setting operation and monitoring.
Protection lid
Protection lid design avoids possible contaminations and environmental heat transfer. This
indirectly helps to keep and maintain the desired temperature of the block cooler system which
could be influenced from the environment. Protection lid handle provides easy handling of the
protection lid.
48-Well aluminum block
48-Well aluminum block is a single block suitable for various types of microtubes which
includes 4 x 0.2ml, 21 x 0.5ml, 15 x 1.5ml and 4 x 2.0ml.
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