Thermo 3413 Manuel utilisateur

Analyze Detect Measure Control™

IEC Micro MB

Centrifuges

IM3411 Rev. 4 11 January 2004

Cat. No.3413: 120 VAC, 50/60Hz (no rotor)

Instruction Manual

Cat. No.3414: 240 VAC, 50/60Hz (no rotor)

Cat. No.3415: 120 VAC, 50/60Hz (837 rotor)

Cat. No.3416: 240 VAC, 50/60Hz (837 rotor)

Cat. No.3411: 120 VAC, 50/60Hz (275 rotor)

Cat. No.3426: 240 VAC, 50/60Hz (275 rotor)

IM3411 Rev. 4 11 January 2004 Page 3 of 16

1. Introduction....................................................... 3

1.1 Product Description ...................................................... 4

1.2 Hematocrit Test (using 275 rotor) ................................. 4

2. Installation......................................................... 4

2.1 Receive the Unit ........................................................... 4

2.2 Prepare the installation site .......................................... 4

2.3 Verify Power Configuration ........................................... 5

2.4 Moving the Unit.............................................................5

3. Operation.......................................................... 5

3.1 Rotor Installation........................................................... 5

3.2 Starting and Stopping a Run ......................................... 6

3.3 Rotor Removal.............................................................. 6

4. Maintenance ..................................................... 7

4.1 Care and Cleaning........................................................ 7

4.2 General ......................................................................... 7

4.3 Brush Replacement ...................................................... 7

4.4 Storage: Keep the Unit Dry ........................................... 7

4.5 Condition of Returned Equipment................................. 7

5. Specifications.................................................... 8

6. Drawings/Parts ................................................. 8

5. Specifications.................................................... 8

Contents

IM3411 Rev. 4 11 January 2004

Page 4 of 16

1. Introduction

1.1 Product Description

The IEC Micro MB is a portable benchtop centrifuge

designed specifically for use as a micro hematocrit

centrifuge (for in vitro diagnostic use) or a microcentrifuge,

depending on which rotor is used.

There are six models:

• Cat. No. 3413 for 120 V, 50/60 Hz (No Rotor)

• Cat. No. 3414 for 240 V, 50/60 Hz (No Rotor)

• Cat. No. 3615 for 120 V, 50/60 Hz (837 Rotor)

• Cat. No. 3616 for 240 V, 50/60 Hz (837 Rotor)

• Cat. No. 3411 for 120 V, 50/60 Hz (275 Rotor)

• Cat. No. 3412 for 240 V, 50/60 Hz (275 Rotor)

The 837 rotor is a 45° fixed angle microtube rotor. The

275 rotor is a 24 place micro hematocrit rotor which

requires a separate reader for reading the hematocrit test

(IEC Reader No. 2201).

The Micro MB is a compact and quiet, single speed

centrifuge that utilizes a power interrupt switch: when the

cover is opened during rotation, power to the motor is

removed. It has a momentary spin feature and a fast

acting manual brake.

1.2 Hematocrit Test (using 275 rotor)

The micro method for determination of hematocrit has been

widely used in clinical laboratories for many years. The

results of micro hematocrit determinations are recognized

as being fully equivalent to the results of macro

determinations. The hematocrit is a quantitive test of the

red cells and the plasma content of a micro sample of

human blood.

The usual micro technique for determining hematocrit

utilizes a small glass capillary tube, internally coated with

an anticoagulant (heparin). This anticoagulant is non-

reactive with the separation of blood cells and plasma and

is placed inside of the capillary tube as a catalytic

preservative. The tube is partially filled with blood obtained

from a finger puncture. One end of the tube is sealed with

plastic clay. The capillary tube is placed in a micro

hematocrit centrifuge where it is spun at high speed for

about 5 minutes. After centrifugation, the blood sample

appears into two major fractions - a column of packed red

cells and a column of clear plasma; with the small fraction

of white cells and platelets between the major fractions.

The hematocrit is defined as the ratio of the volume of

packed red cells to the volume of the original sample,

multiplied by 100. Since the capillary tube is of uniform

bore, the hematocrit may be easily determined by

measuring the length of the column of packed red cells

against the overall length of the blood sample. The

hematocrit value may then be redefined as follows:

Usually the two lengths are measured with the aid of

special mechanical reading devices, such as the IEC reader

(Cat. No. 2201). Such devices present the final hematocrit

value without requiring any computation by the operator.

HEMATOCRIT= length of column of packed red cell

overall length of blood sample X 100

2. Installation

2.1 Receive the Unit

All units are shipped in protective packaging.

1. Follow the unpacking instructions on the carton.

2. Inspect the unit upon receipt and immediately file any

damage claims with the shipper/carrier.

3. Complete and return the postage-paid warranty card.

2.2 Prepare the installation site

The unit normally resides on a bench top.

1. Place the centrifuge on a clean, dry surface, to make

certain that the suction feet at the bottom grip the

surface firmly. Keep the area beneath the unit free of

debris and loose materials.

CAUTION

The resting surface must be level, to ensure quiet,

vibration-free operation. A rigid and stable location is

important. An improperly loaded centrifuge may

vibrate or move

2. Locate the centrifuge to allow a clearance of 8 cm

(3 inches) on all sides of the unit, to ensure proper

ventilation.

WARNING

International Electrotechnical Commission standard

1010 part 2-20 limits the permitted movement of a

laboratory centrifuge to 12” (300 mm) in the unlikely

event of a disruption. Laboratory management

procedures should require that no person or any

hazardous materials enter within this boundary while

the centrifuge operates.

IM3411 Rev. 4 11 January 2004 Page 5 of 16

2.3 Verify Power Configuration

1. Verify that the correct power cord and connector is

provided for your installation.

The unit requires a grounded power supply (3-outlet).

If your facility does not have grounded power outlets,

arrange for proper grounding. The power cord plugs in

on the left side of the unit.

WARNING

ELECTRICAL HAZARD!

Do not remove the grounding pin from the

centrifuge power cord. Do not use the bare

wired power cord to attach a power plug that

does not have a grounding pin.

The power cord provided with the unit is

correctly rated for the highest current demand.

This power cord should not be interchanged

with cords from equipment with lower current

demand. Exchange of power cords between

equipment may create a fire hazard.

2. For best results, the refrigerated centrifuge should be

used on a dedicated line. Variations in line voltage or

frequency affect the unit’s speed and other

characteristics.

NOTE:

Less than nominal line voltage may prevent the

centrifuge from reaching maximum published

specifications of speed and/or temperature. Power line

voltage, at some locations, may sag when the

refrigeration system turns on

2.4 Moving the Unit

Suction cups at the bottom of the unit keep it anchored to

the work surface. Keeping the unit stationary is a safety

feature.

To move the unit to a new location:

WARNING

Use caution when moving to avoid any injury.

1. Check that the new site meets the criteria in Section

2.2 before moving the unit.

2. Position a flat object, such as a tongue depressor, near

a suction cup at the bottom of the unit.

3. Lift up an edge of the cup, and insert the flat object far

enough to break the vacuum suction seal.

4. When all four suction cups are disengaged, lift the unit

from the work surface.

5. When the unit is in its new location, ensure that the

suction cups adhere correctly to the work surface

3. Operation

WARNING

Do not open the cover before the rotor comes to a

complete stop.

CAUTION

For proper operation, always load the rotor

symmetrically with a full (or at least paired) set of

tubes. Verify that each tube is filled equally (by weight

or at least visually) to its corresponding opposite.

CAUTION

Do not block the vents because airflow will be restricted

and overheating may take place.

3.1 Rotor Installation

1. Open the latch and lift the cover of the centrifuge.

2. Remove any locking nut that may be located on the

head adapter (threaded shaft).

3. Place a rotor (275 or 837) onto the adapter making sure

it engages the drive pins of the adapter. To do so, place

one hand on the rotor and one hand on the shaft.

Rotate the rotor and the shaft in opposite directions

until you feel the rotor drop down onto the drive pins.

Install the rotor locking nut onto the threaded shaft and

tighten it using the spanner wrench.

275 Rotor

When using a 275 rotor it is first necessary to install the

1505 cushion.

1. Place the cushion so that it lines the inner side of the

flange of the rotor making a smooth butt-joint with the

ends. (It may be necessary to cut off a short piece of

excess length to obtain the correct fit.) A thin layer of

vacuum grease (IEC Part No. 2084) on the outer side of

the cushion will provide sufficient adhesion to hold it in

place and will facilitate replacement. Occasional shifting

of the cushion will be necessary as it becomes

punctured with use.

CAUTION

When loading hematocrit tubes into the 275 rotor,

make sure that all tubes are touching the rubber

cushion and that the other ends of the tubes do not

protrude into the center recess in the rotor where the

locking nut fits.

2. Place the rotor cover onto the adapter and tighten the

knob onto the rotor until it is finger tight.

837 Rotor

Adapters come with each 837 rotor to allow smaller

microtubes to be used.

The rotor directly accepts 11 mm diameter, 1.5 or 2.0 ml

plastic microtubes.

• Use the black plastic adapters (IEC Part No. 5763) when

using 8 mm diameter B-D (Becton Dickinson)

Microtainers and 0.5 ml tubes.

• Use the white plastic adapters (IEC Part No. 5764)

when using 6 mm diameter 0.4 and 0.25 ml tubes.

IM3411 Rev. 4 11 January 2004

Page 6 of 16

Balance

Install equal (by weight or by visual inspection) samples

(including any adapters) in pairs across from each other in a

rotor. Different adapters or tubes may be used in the same

run provided they are balanced around the rotor as though

they were the only samples in the rotor.

3.2 Starting and Stopping a Run

1. Plug the centrifuge in.

2. Close and latch the cover.

3. The centrifuge can be run in a Timed or Untimed Mode.

• To run in the Timed Mode:

Turn the timer knob clockwise to the desired setting.

The run will start as soon as the timer knob is turned

clockwise. Select a time up to 15 minutes in 30 second

increments. If the desired run time is less than 5

minutes, turn the timer knob clockwise to the 5 minute

mark and then back to the desired time. Changing the

timer knob setting during a run affects the run in

progress. When the timer knob returns to the 0 minute

position, a signal bell sounds and the rotor coasts to a

stop.

• To run in an Untimed Mode:

Set the timer knob to 0 minutes and press and hold the

Momentary Spin button. The run will start as soon as

this button is pressed, and will end as soon as it is

released.

NOTE: In either Mode, if faster stopping is desired, hold

the brake switch down until the rotor has almost come

to rest (approximately 5 seconds) and release the brake

switch. The rotor can be seen through the top vent in

the cover of the centrifuge.

Note:The rotor will reverse direction of rotation if the

brake switch is held down after the rotor stops.

WARNING

Do not open the cover before the rotor comes to a

complete stop.

Note:The power will be interrupted and the brake

switch will not function if the cover is opened.

4. Once the rotor has come to a complete stop, unlatch

and lift the cover.

3.3 Rotor Removal

To remove the rotor, use the spanner wrench (Thermo

Electron Part No. 1883) to loosen the locking nut(s) and lift

the rotor off of the motor shaft.

IM3411 Rev. 4 11 January 2004 Page 7 of 16

4. Maintenance

4.1 Care and Cleaning

• Keep the centrifuge clean to ensure good operation and

to extend its life.

• Clean the sample chamber, rotor, and lid, at the end of

each work day, and immediately after any spill. Use a

damp sponge, warm water, and a mild liquid detergent,

suitable for washing dishes by hand, such as Ivory®

liquid. Do not use caustic detergents or detergents that

contain chlorine ions. These attack metals.

CAUTION

Do not use caustic or acid cleaning agents. The 275

rotor will corrode when exposed to sodium or

chlorine ions.

• Remove stubborn stains with a plastic scrub pad. Do

not use steel wool, wire brushes, abrasives, or

sandpaper as they create corrosion sites. Never pour

water directly into the rotor chamber.

• Scrub the rotor’s tube cavities with a stiff test tube

brush that has end bristles and a non-metallic tip. After

cleaning, dry each part with a clean absorbent towel.

CAUTION

In the case of glass breakage, be careful to remove

ALL particles of glass from the unit! In particular, be

sure thoroughly scrub adapters and cushions with a

wire brush or replace these items as glass shards

embedded in the adapters can cause further

breakage.

4.2 General

The Micro MB motor requires no lubrication. The motor

bearings are sealed, permanently lubricated bearings.

The Micro MB requires no calibration. Verification of speed

is recommended every six months. The minimum speed of

the centrifuge is 11,600 RPM.

If the centrifuge does not start, check that the cover

switch is engaged when the cover is latched. If it does not

engage, the latch can be tightened by adjusting the screw

inside the latch assembly. When properly adjusted, a

closed cover will compress the gasket slightly and the latch

will be snug. If the latch is snug but a gap still exists

between the cover and the gasket, the gasket must be

replaced.

4.3 Brush Replacement

The motor brushes should be inspected every 3-4 months

under normal use, and more often with heavy use.

1. Unplug the centrifuge, remove the rotor, and place the

unit on a benchtop upside down.

2. Remove the three screws from the baseplate and

remove the baseplate.

3. Unscrew the motor brush caps and remove the brushes.

If the carbon portion of the brush is 1/4 inch or less,

replace the brushes. When replacing the brushes,

spread the metal clip on the end of the brush spring

slightly, so that it will stay in the brush holder until the

cap is screwed in. Order a pair of replacement brushes

as IEC Part No. 1840. (A pair of brush caps is IEC Part

No. 30895).

4.4 Storage: Keep the Unit Dry

Store parts on a soft surface to avoid damage.

Rotors and other parts should be clean and dry. Store them

open to the air, not in a plastic bag, so any residual

moisture evaporates. Face the parts upward to avoid

moisture retention in the cavities.

4.5 Condition of Returned Equipment

Obtain a return goods authorization (RGA) before returning

equipment to the manufacturer. The RGA paperwork

includes a Certificate of Decontamination for you to sign.

It indicates that you have performed the proper steps for

decontaminating the unit.

WARNING

All returned units must be decontaminated, free of

radioactivity, and free of hazardous, infectious,

pathogenic, or toxic materials.

All return equipment shipments will be refused until the

signed certificate is received.

You must prepay transportation to the service depot.

IM3411 Rev. 4 11 January 2004

Page 8 of 16

5. Specifications

Rotation Speed 11,600 RPM minimum

14,000 RPM maximum

Relative Centrifugal

Force

13,200 xg (275 rotor) minimum

8,700 xg (837 rotor) minimum

19,200 xg (275 rotor) maximum

12,700 xg (837 rotor) maximum

Acceleration Less than 10 seconds

Deceleration Less than 5 seconds (brake)

Approx. 45 seconds (coast)

Capacity 12 x 2.0 ml (837 rotor)

24 x Hematocrit Tubes (275 rotor)

Timer

Range 0 to 15 minutes

Increments 30 seconds

Accuracy ± 5% full scale

Power Requirements and Output

Voltage

120 VAC ± 10%, 50/60 Hz

(Cat. No. 3411, 3613, 3615)

240 VAC ± 10%, 50/60 Hz

(Cat. No. 3412, 3614, 3616)

Power Consumption 140 Watts (operating)

Heat Output (typical) 480 Btu/hr

Dimensions

Cover Closed Height: 229 mm ( 9 in.)

Cover Open Height: 457 mm (18 in.)

Width 254 mm (10 in.)

Depth 279 mm (11 in.)

Unit Weight: 9.1 kg (20 lbs.)

Shipping Weight: 10.9 kg (24 lbs.)

Specifications subject to change without notice.

6. Drawings/Parts

Item

No. Description 120V 240V

1 Base 49780

3 Brake Resistor 41313

4 Harness - Power 49793

5 Harness - Brake Res. 49794

7 Ground Lead 33790

8 Motor 44890 44891

9 Head Adapter 7915

10 Switch (Latch) 49784

11 Gasket (Cover) 1511

13 Stud 30898

14 Grommet 9660

15 Screen 30889

16 Spanner Wrench 1883

17 Coupling 30901

18 Power Cord 38805 46759

19 Bead Chain 7907

20 Foot 46618

21 Washer 30888

22 Base Plate 30877

23 Timer 768

24 Screw 65455

25 Spacer 30882

26 Latch Pin 30887

27 Latch 30878

28 Switch (Spin) 49789

29 Cover 49771B

30 Front Panel 49788

31 Switch (Brake) 9942

32 Timer Knob 30839

33 'S' Hook 30902

34 Liner 42991

35 Insulation 31675

37 Motor Dampener 30992

38 Vinyl Tip 45390

39 Knurled Nut 42011

40 Vent Screen 30900

43 Washer 49224

66 Label 50012

70 Label 46625

IM3411 Rev. 4 11 January 2004 Page 9 of 16

IM3411 Rev. 4 11 January 2004 Page 10 of 16

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