Thermo Scientific MDrop Manuel utilisateur

Cat. No. N12691 Rev 4.0 2020

Thermo Scientific

μDrop and μDrop Duo

Plates

User Manual

documentation in whole or in part is prohibited

Manufacturer

Life Technologies Holdings Pte. Ltd.

(a part of Thermo Fisher Scientific Inc.)

33, Marsiling Industrial Estate Road 3, #7-06,

Singapore 739256

Trademarks

“μDrop” is a trademark of Thermo Fisher Scientific. “Multiskan”, “SkanIt” and “Varioskan” are registered

trademarks of Thermo Fisher Scientific.

All other trademarks and registered trademarks are the property of their respective holders.

Disclaimer

Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate

technological developments. This manual is subject to change without prior notice as part of continuous

product development. Although this manual has been prepared with every precaution to ensure accuracy,

Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from

the application or use of this information. This manual supersedes all previous editions.

No liability for consequential damages

Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use

this product.

Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual i

Preface

About This Guide

This manual gives instructions on performing absorbance measurements using the Thermo Scientific™

μDrop™ and μDrop™ Duo Plates with the following instruments:

• Thermo Scientific™ Multiskan™ SkyHigh

• Thermo Scientific™ Multiskan™ Sky

• Thermo Scientific™ Multiskan™ GO

• Thermo Scientific™ Varioskan™ LUX

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and other

special notices appear in boxes.

The following symbols and markings appear in this user manual:.

WARNING Biohazard risk.

WARNING Risk of injury to the user or users.

CAUTION Risk of damage to the instrument, other equipment or loss of performance or function

in a specific application.

Note Highlights important information, a tip, or an item of interest that is useful in the optimum

operation of the system

About This Guide

ii Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific

Contacting Us

For the latest information on products and services, visit our website at:

www.thermofisher.com/platereaders

Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual iii

Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i

About This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i

Safety and Special Notices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i

Contacting Us. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ii

Chapter 1 Introduction to the μDrop and μDrop Duo Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

General description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Chapter 2 Low-volume measurements with SkanIt Software . . . . . . . . . . . . . . . . . . . . . . . . . 5

Creating a new measurement session . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Ready-made sessions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

User-made sessions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Placing the samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Cleaning verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Chapter 3 Cuvette measurements with SkanIt Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Placing the cuvette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Creating a new measurement session . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

User-made sessions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Chapter 4 Setting up a measurement from the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Regular and preventive maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

μDrop and μDrop Duo Plates care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Cleaning the μDrop and μDrop Duo Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Disposal of materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Decontamination procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Disposal of the μDrop and μDrop Duo Plates . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Chapter 6 Technical specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

General specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Contents

iv Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific

Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual 1

Introduction to the μDrop and μDrop Duo Plates

Introduction

Thermo Scientific™ μDrop™ and μDrop™ Duo Plates are tools designed for quick and easy absorbance

measurement of low sample volumes (2–10 μl) and cuvettes with the following instruments:

• Thermo Scientific™ Multiskan™ SkyHigh

• Thermo Scientific™ Multiskan™ Sky

• Thermo Scientific™ Multiskan™ GO

• Thermo Scientific™ Varioskan™ LUX

The μDrop and μDrop Duo Plates are typically used for photometric DNA, RNA and protein

quantitation and purity analysis.

The μDrop Plate consists of two separate measurement locations, one for measuring low-sample

volumes and the other for cuvettes. The μDrop Duo Plate consist of three separate measurement

locations, two for measuring low-sample volumes and one for cuvettes.

The low-volume area of the μDrop and μDrop Duo Plates is compatible with an 8-channel pipette to

enable easy pipetting of the samples to the μDrop and μDrop Duo Plates. It can be used for endpoint

and spectral measurements.

The cuvette slot is compatible with standard rectangular cuvettes with a stopper. It can be used for

endpoint, spectral and kinetic measurements.

Intended use

The μDrop and μDrop Duo Plates enable quick and easy absorbance measurements with low sample

volumes and standard rectangular cuvettes with a stopper. The main applications are photometric

nucleic acid and protein analysis.

The μDrop and μDrop Duo Plates are intended to be used in research laboratories by professional

personnel.

The μDrop and μDrop Duo Plates are designed to be a part of an analyzing system for the end user,

who is responsible for validating the system to ensure reliable and safe results. We recommend using

Good Laboratory Practice (GLP) during the analysis process.

1Introduction to the μDrop and μDrop Duo Plates

General description

2Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific

General description

The μDrop Plate low-volume measurement area on the left-hand side consists of two quartz slides, the

top clear quartz and the bottom Teflon-coated quartz slide (see Figure 2). The bottom slide contains 16

sample positions, arranged in a 2 x 8 matrix, where samples are to be pipetted. The nominal pathlength

for the sample is fixed to 0.5 mm. The verified pathlength for each μDrop Plate is indicated on the

quality control measurement report delivered with the μDrop Plate.

The μDrop Duo Plate has two low-volume measurement areas on the left-hand side (see Figure 4). The

verified pathlengths for each μDrop Duo Plate are indicated on the quality control measurement report

delivered with the μDrop Duo Plate.

On the right-hand side, the μDrop and μDrop Duo Plates have a slot for cuvette measurement (see

Figure 1 and Figure 3).

Figure 1. A closed μDrop Plate showing the low-volume measurement area (1), adjustment screw (2), and

the cuvette measurement point (3).

1 Introduction to the μDrop and μDrop Duo Plates

General description

Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual 3

Figure 2. An open μDrop Plate showing the top slide (1), bottom slide (2), sample positions A2 - H3 (2 x 8

matrix) (3), low-volume area (4), and cuvette slot (5).

Figure 3. A closed μDrop Duo Plate showing the low-volume measurement area (1), adjustment screw (2),

and cuvette measurement point (3).

1Introduction to the μDrop and μDrop Duo Plates

General description

4Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific

Figure 4. An open μDrop Duo Plate showing the top slides (1), bottom slides (2), sample positions (two 2 x

8 matrixes) (3), low-volume area (4), and cuvette slot (5).

The measurement sessions for Multiskan SkyHigh, Multiskan Sky, Multiskan GO and Varioskan LUX

can be created using Thermo Scientific™ SkanIt™ Software. It is also possible to measure the μDrop and

μDrop Duo Plates with Multiskan SkyHigh and Multiskan Sky internal software ready-made

protocols.

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