Thermo Scientific F1-ClipTip Manuel utilisateur

Thermo Scientiﬁc F1-ClipTip

Single Channel

Multichannel

Instructions for Use

User Manual

This product complies with the European Union Directive 98/79/EC, and it is marked with

a CE marking.

When the product is used in applications related to the Directive 98/79/EC, read the

additional information enclosed in the product package or contact the manufacturer at

info.pipettes@thermoﬁsher.com to ensure correct and safe use.

The CE mark covers the system containing the CE marked F1-ClipTip pipette and the CE

marked ClipTip pipette tips.

Product speciﬁcations are subject to change without prior notice. ClipTip is a registered

trademark of Thermo Fisher Scientiﬁc Inc. and its subsidiaries.

CONTENTS

1. INTRODUCTION 4

2. PACKAGE 5

3. MAIN ILLUSTRATION 5

4. SAFETY 6

5. OPERATION 7

6. CALIBRATION AND ADJUSTMENT 9

7. MAINTENANCE 11

8. STERILIZATION 16

9. TROUBLESHOOTING 17

10. APPENDICES 18

1. Introduction

Congratulations on your purchase of a Thermo Scientiﬁc®F1-ClipTip®pipette!

The F1-ClipTip is a general purpose air displacement micropipette. Its intended use is for aspirating

and dispensing liquids.

The F1-ClipTip features an innovative interlocking tip interface technology that locks the tip in place

ensuring secure tip attachment and preventing tips from being displaced in the middle of pipetting.

F1-ClipTip operates only with ClipTip®tips. For improved ergonomics, tips are attached and ejected

using minimal application pressure.

The F1-ClipTip product offering covers a volume range from 1 µl to 1000 µl and includes variable and

ﬁxed volume single channel pipettes as well as multichannel pipettes. The convenient color coding*

system facilitates to identify the correct ClipTip by volume for use.

F1-ClipTip Variable Volume Single Channel

Cat. no. Description Volume range Increment Color code Compatible

ClipTip

4641170 F1-ClipTip 1-10 µl 1–10 µl 0.02 µl pink ClipTip 20

4641180 F1-ClipTip 2-20 µl 2–20 µl 0.02 µl pink ClipTip 20

4641190 F1-ClipTip 5-50 µl 5–50 µl 0.1 µl violet ClipTip 50

4641200 F1-ClipTip 10-100 µl 10–100 µl 0.2 µl yellow ClipTip 200

4641210 F1-ClipTip 20-200 µl 20–200 µl 0.2 µl yellow ClipTip 200

4641220 F1-ClipTip 30-300 µl 30–300 µl 1 µl orange ClipTip 300

4641230 F1-ClipTip 100-1000 µl 100–1000 µl 1 µl blue ClipTip 1000

F1-ClipTip Fixed Volume Single Channel

Cat. no. Description Volume range Color code Compatible ClipTip

4651170 F1-ClipTip 1 µl 1 µl dark blue ClipTip 20

4651180 F1-ClipTip 5 µl 5 µl dark blue ClipTip 20

4651190 F1-ClipTip 10 µl 10 µl dark blue ClipTip 20

4651200 F1-ClipTip 20 µl 20 µl dark blue ClipTip 50

4651210 F1-ClipTip 25 µl 25 µl dark blue ClipTip 50

4651220 F1-ClipTip 50 µl 50 µl dark blue ClipTip 50

4651230 F1-ClipTip 100 µl 100 µl dark blue ClipTip 200

4651240 F1-ClipTip 200 µl 200 µl dark blue ClipTip 200

4651250 F1-ClipTip 250 µl 250 µl dark blue ClipTip 300

4651260 F1-ClipTip 500 µl 500 µl dark blue ClipTip 1000

4651270 F1-ClipTip 1000 µl 1000 µl dark blue ClipTip 1000

* color coding does not apply to ﬁxed models

F1-ClipTip Multichannel

Cat. no. Description Channels Volume Increment Color code Compatible

range ClipTip

4661110 F1-ClipTip 8-ch 1–10 µl 8 1–10 µl 0.02 µl pink ClipTip 20

4661120 F1-ClipTip 8-ch 5–50 µl 8 5–50 µl 0.1 µl violet ClipTip 50

4661130 F1-ClipTip 8-ch 10–100 µl 8 10–100 µl 0.2 µl yellow ClipTip 200

4661140 F1-ClipTip 8-ch 30–300 µl 8 30–300 µl 1 µl orange ClipTip 300

4661150 F1-ClipTip 12-ch 1–10 µl 12 1–10 µl 0.02 µl pink ClipTip 20

4661160 F1-ClipTip 12-ch 5–50 µl 12 5–50 µl 0.1 µl violet ClipTip 50

4661170 F1-ClipTip 12-ch 10–100 µl 12 10–100 µl 0.2 µl yellow ClipTip 200

4661180 F1-ClipTip 12-ch 30–300 µl 12 30–300 µl 1 µl orange ClipTip 300

1Pipetting and volume adjustment button

2Adjustable ﬁnger rest

3Tip ejector

4Volume display

5Space for personal ID tag

6Tip ﬁtting

7Tip sealing O-ring

8Color coding

2. Package

The F1-ClipTip package contains the following items:

1. F1-ClipTip pipette

2. Service tool

3. Multichannel service tool

4. Tip ﬁtting tool

5. ClipTip tip sample

6. Tube of grease (Order No. 2203130)

7. Instructions for Use

8. Calibration certiﬁcate / Warranty Certiﬁcate

9. Shelf hanger (Order No. 2206040)

10. Warning labels

11. O-ring Order No. 2214930 Single Channel Pipette (Scp) 300 (3 pcs)

Order No. 2215540 Scp 1000 (3 pcs)

Order No. 2214920 Multichannel Pipette (Mcp) (12 pcs)

3. Main Illustration

3.1. Materials

The F1-ClipTip is made of mechanically durable and chemically resistant materials.

The F1-ClipTip pipette includes components which contain the antimicrobial additive, silver sodium

hydrogen zirconium phosphate. Silver is known to inhibit the growth of a broad spectrum of micro-

organisms. For waste disposal instructions, contact your local environmental agency. For more

information, contact us at info.pipettes@thermoﬁsher.com.

Common laboratory disinfectants, such as 70 % ethanol, can be used without effect on antimicrobial

treatment.

Aggressive substances can damage the pipette or pipette parts. Check for material

compatibility before using organic solvents and aggressive chemicals.

ClipTip pipette tips are made of natural color virgin polypropolyene. The tips feature a hydrophobic

surface, which effectively prevents liquid retention. ClipTips pipette tips come in multiple packaging

options including racks and reload packs, in sterile and non-sterile versions. ClipTip tips are also available

with a ﬁlter and are certiﬁed free of human DNA, RNase, DNase, ATP and endotoxin contamination.

The material used in F1-ClipTip pipettes and ClipTip tips are as follows:

F1-CLIPTIP PIPETTES

Material Component

External surfaces of the upper parts:

ABS/PC acrylonitrilebutadienstyrene/polycarbonate

PA polyamide

PEI polyetherimide

Foil

Exterior and interior of lower parts:

PEI polyetherimide

PA polyamide

PVDF polyvinyldenﬂuoride

EPDM ethylene-propylene-diene rubber

FVM ﬂuorosilicone rubber

Steel stainless steel

CLIPTIP TIPS

Tip PP polypropylene

Filters PE polyethylene

4. Safety

Cautions are marked with this symbol .

4.1. Intended use

The intended use of the device is to transfer liquids in the volume range of 1 µl to 1000 µl.

The F1-ClipTip pipette and ClipTip tip are designed as a component of an analyzing system for an end

user, who is responsible for validating the system to ensure reliable and safe results.

Damage to health

Follow general procedures for hazard prevention and safety instructions; e.g. wear

protective clothing, eye protection and gloves.

For use and waste disposal of hazardous (e.g. radioactive and potentially infectious) material,

follow the safety instructions and general laboratory practice.

The pipette and tips are not intended for in vivo use. Do not use the pipette for pipetting any liquid

to be injected into a human body.

Do not eject the tip towards anybody.

The F1-ClipTip is to be used by trained personnel with required laboratory skills. The instructions for

use must be read prior to and during the use of the device (pipette and tip). The F1-ClipTip pipette can

be used between +4°C and +40°C.

Incorrect dispensing results

Performance may vary due to:

a. pipetting method (forward pipetting technique recommended)

b. temperature (air, liquid, vessel, pipette, and tip)

c. pressure

d. humidity

e. operator, e.g. thumb movement, pipetting angle

f. liquid density, viscosity and vapor pressure

g. type of tip

If the pipetting performance is critical to the outcome of a speciﬁc application, the result has to

be assured with an alternative test, and if this is not an option, by duplicate testing.

The possibility of an incorrect volume delivery during pipetting cannot be entirely mitigated.

To avoid inaccurate dispensing and/or leakage, check that the tip is properly attached to

the pipette.

When rotating the volume adjustment button, do not exceed the volume range of the pipette.

Otherwise the pipette may be damaged or affect pipetting performance.

Pipetting performance cannot be guaranteed if the tip is reused.

Choose only a tip and pipette with matching color coding.

5. Operation

Cautions for incorrect operation

For optimum performance, make sure that the tips, pipette and solution are at the same

temperature.

Before you begin your actual pipetting, ﬁll and empty the tip three to ﬁve times with the

solution that you will be pipetting.

Push and release the pipetting button slowly at all times particularly when working with high

viscosity liquids. Never allow the pipetting button to snap back.

Do not hold the pipette horizontally or upside down when there is liquid inside the tip. Sample

liquid may enter the pipette, which will affect the pipetting performance and may damage

the pipette.

Operate the pipetting button gently. If it is quickly released, sample liquid may enter the pipette,

which will affect the pipetting performance and may damage the pipette.

Follow the ergonomic guidelines for laboratory work to minimize the risk of repetitive strain

injury (RSI).

5.1 ID-tag

You can mark the pipette ID-tag with your initials, pipetting application or calibration date. Remove

the pipette’s module to replace the tag. Mark the label and slide the tag together with the holder

back to its slot (Picture 1).

5.2 Adjusting the ﬁnger rest

The ﬁnger rest supports the position of the pipette on the index ﬁnger, enhancing ergonomics. The

ﬁnger rest can be adjusted by rotating it 60 degrees in both directions from the center position

(Picture 2). Usually right-handed operators turn it left (counter clockwise) to achieve the best possible

position for the thumb to eject the tip.

Picture 1 Picture 2

5.3 Setting the delivery volume

1. Set the delivery volume using the volume adjustment

button on the top of the pipette (Picture 3). Pull the

button to activate the volume setting.

2. To increase the delivery volume, turn the button

counterclockwise. To decrease the delivery volume,

turn it clockwise.

3. Lock the volume by pushing the button down.

4. Check the volume on the display.

NOTE! Do not set volumes outside the pipette´s speciﬁed

volume range. Using excessive force to turn the volume

adjustment button outside the range may jam the mechanism

and eventually damage the pipette.

5.4 Tip attachment

1. To attach the tip, guide the pipette into a ClipTip in a rack

and press with light force until the tip is attached. A delicate

click sound will indicate that the tip is attached. The tip is

attached when the clips* (Picture 4) are locked to the tip ﬁtting.

Do not use excessive force when attaching the tips as the

device requires minimum attachment force.

2. Lift the pipette.

3. If the tip is not attached, repeat Steps 1 and 2.

Incorrect use of tips

Use only ClipTip tips with an F1-ClipTip pipette.

Check that all clips are locked to ensure tip sealing.

ClipTip tips are designed for single use only.

To ensure sterility and purity of tips check that the package is unbroken. For sterile tips also

ensure that the indicator dot is red.

Check that the tip is complete and unbroken. Check for foreign particles in the tip.

Check that the ﬁlter tips have ﬁlters in place and that each ﬁlter is unbroken and not tilted.

5.5 Aspirating and dispensing liquid

1. Fill a suitable vessel with the liquid to be dispensed.

2. Depress the pipetting button to the ﬁrst stop (Picture 5).

3. Dip the tip under the surface of the liquid

in the reservoir to a depth of about 1 cm

and slowly release the button.

Withdraw the tip from the liquid by moving

it against the edge of the reservoir to

remove excess liquid.

4. Deliver the liquid by gently depressing the

button to the ﬁrst stop. After a delay of

about one second, continue to depress the

button all the way to the second stop. This

action will empty the tip.

5. Withdraw the tip by moving it against the edge of the target container.

Ensure proper functioning of F1-ClipTip 1-10µl multichannel and 1-50 µl

single channel pipettes

The F1-ClipTip 1-10µl multichannel and 1-50 µl single channel pipettes utilize an innovative super

blow-out piston technology to ensure efﬁcient liquid delivery with small volumes. In pipettes with

the super blow-out piston three separate movements are observed (Picture 6) when the plunger is

pressed down to dispense liquid:

1. Primary plunger movement – delivers the set volume amount

2. Blow-out – additional phase to dispense residual liquid

3. Super blow-out – enhances the effectiveness of blow-out

Picture 4

1 2 3 4

Picture 5

Picture 3

When the pipette is not used for a period of time, the plunger movement may become stiff due to

hardening of grease. In rare cases this can affect the functionality of the super blow-out piston,

which can become temporarily jammed. To ensure proper functionality it is recommended to press

the plunger several times to the ﬁnal stop (step 3 in picture) before dosing the sample liquid. This

releases the piston and re-distributes the lubricant.

NOTE! If the super blow-out piston is jammed, increased force might be required to release it. Ensure

that plunger moves to the down position (Picture 6).

For more information about different pipetting techniques, check the Good Laboratory Pipetting

Guide available at www.thermoscientiﬁc.com/GLP.

5.6 Tip ejection

To release the tip, point the pipette at a suitable waste receptacle and press the tip ejector with

your thumb.

6. Calibration and Adjustment

6.1 Factory calibration limits

F1-ClipTip pipettes are factory calibrated and adjusted to give the volumes as speciﬁed with distilled

or deionized water using the forward pipetting technique. Manufacturer speciﬁcations are shown on

the pipette calibration certiﬁcate and in Appendix 2.

Cautions for calibration and pipette adjustment

It should be noted that the use of different pipetting techniques may affect the calibration

results. The pipettes are constructed to permit re-adjustment for other pipetting techniques

or liquids of different temperature and viscosity.

The performance of a new and serviced pipette must be checked and documented.

The manufacturer’s speciﬁcations should be used as guidelines and the user should establish

acceptable imprecision and inaccuracy performance limits (ISO 8655).

The user determines the performance and applicability of the pipette and tip combination

for a particular application and determine the required interval for performance checking.

Before starting the actual dosing, pre-rinse the tip with the liquid by ﬁlling and emptying the

tip three to ﬁve times to improve accuracy and precision.

6.2 Test conditions and equipment

An analytical balance must be used. The scale graduation value of the balance should be chosen

according to the selected test volume of the pipette:

Volume range Readable graduation

under 10 µl 0.001 mg

10–100 µl 0.01 mg

above 100 µl 0.1 mg

The test liquid is deionized distilled “grade 3” water in compliance with ISO 3696. Tests are done in a

draft-free room at a constant (±0.5°C) temperature between 15°C to 30°C. The relative humidity must

be above 50%. Especially with volumes under 50 µl, air humidity should be as high as possible to reduce

the effect of evaporation loss. Special accessories, such as an evaporation trap, are recommended.

Plunger in down position

Plunger in up position

Picture 6

6.3 Procedure to check calibration

The pipette is checked at maximum volume (nominal volume) and minimum volume. A new tip is

ﬁrst pre-wetted three to ﬁve times and a series of ten pipettings is done at both volumes. A pipette

is always adjusted according to the delivery of the selected volume. Use of the forward pipetting

technique is recommended. The maximum permissible errors are valid for the forward pipetting

method. It is recommended to use the ISO8655 calibration limits in Appendix 3 or user-deﬁned

speciﬁcations.

Procedure:

1. Do 10 pipettings at the minimum volume.

2. Do 10 pipettings at the maximum volume.

3. Calculate the inaccuracy (A) and imprecision (CV) of both series.

4. Compare the results to the limits.

If the calculated results are within the selected limits, the adjustment of the pipette is correct.

Pipette adjustment

Adjustment is done only for one volume. The recommended adjustment volume is the minimum

volume or 10% of the maximum volume.

1. Fit the service tool (Picture 7) into the openings of the

calibration nut at the top of the handle.

2. Turn the service tool clockwise to increase, or

counterclockwise to decrease the volume.

3. After adjustment, check the calibration according to

the instructions above.

Formulas for calculating results

Conversion of mass to volume

V = (w + e) x Z V = volume (µl)

w = weight (mg)

e = evaporation loss (mg)

Z = conversion factor for µl/mg conversion

Evaporation loss can be signiﬁcant at low volumes. To determine mass loss, dispense water into the

weighing vessel, note the reading and start a stopwatch. Check and note down how much the reading

decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).

Compare this to the pipetting time from taring to reading. Typically , the pipetting time might be 10

seconds and the mass loss is then 2 mg (10 s x 0.2 mg/s in this example). If an evaporation trap or lid

on the vessel is used, the correction of evaporation is usually unnecessary.

The factor Z is for converting the weight of the water to volume at the test temperature and pressure.

A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table in Appendix 1.

Inaccuracy (systematic error)

Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.

A = V - V0A = inaccuracy

V = mean volume

0= nominal volume

Inaccuracy can be expressed as a relative value: A% = 100% x A / V0

Imprecision (random error)

Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation (S) or

coefﬁcient of variation (CV)

S = standards deviation

V = mean volume

n = number of measurements

Standard deviation can be expressed as a relative value: CV = 100% x S / V

Picture 7

Autres manuels pour F1-ClipTip

Ce manuel convient aux modèles suivants

Table des matières

Autres manuels Thermo Scientific Équipement de laboratoire

Thermo Scientific

Thermo Scientific SUPER-NUOVA+ Manuel utilisateur

Thermo Scientific

Thermo Scientific Matrix 1029 Manuel utilisateur

Thermo Scientific

Thermo Scientific EASY-Spray Series Manuel utilisateur

Thermo Scientific

Thermo Scientific AquaSensors AquaTrace Manuel utilisateur

Thermo Scientific

Thermo Scientific Locator Jr Manuel utilisateur

Thermo Scientific

Thermo Scientific Orbitrap Fusion Lumos Manuel utilisateur

Thermo Scientific

Thermo Scientific TSQ 9000 Manuel utilisateur

Thermo Scientific

Thermo Scientific FB1300 Manuel utilisateur

Thermo Scientific

Thermo Scientific KingFisher Flex Manuel utilisateur

Thermo Scientific

Thermo Scientific iCAP 7000 Series Manuel utilisateur

Thermo Scientific

Thermo Scientific S1 Manuel utilisateur

Thermo Scientific

Thermo Scientific MS/ETD Manuel utilisateur

Thermo Scientific

Thermo Scientific Pierce G2 Manuel utilisateur

Thermo Scientific

Thermo Scientific Dionex ICS-5000+ Manuel utilisateur

Thermo Scientific

Thermo Scientific Heraeus Megafuge 16 Centrifuge Manuel utilisateur

Thermo Scientific

Thermo Scientific Jewett Manuel utilisateur

Thermo Scientific

Thermo Scientific LH-4000W Manuel utilisateur

Thermo Scientific

Thermo Scientific Wellwash Versa Manuel utilisateur

Thermo Scientific

Thermo Scientific Lindberg/Blue M Mini-Mite TF55030 Manuel utilisateur

Thermo Scientific

Thermo Scientific Evolution 200 Series Manuel utilisateur

Thermo Scientific

Thermo Scientific mySPIN 12 Manuel utilisateur

Thermo Scientific

Thermo Scientific Nicolet Summit Manuel utilisateur

Thermo Scientific

Thermo Scientific Orion 2111XP Manuel utilisateur

Thermo Scientific

Thermo Scientific Multidrop Combi+ Manuel utilisateur

Manuels Équipement de laboratoire populaires d'autres marques

Qiagen

Qiagen DML 3000 Manuel utilisateur

Queensgate

Queensgate NANOSCAN OP400 Manuel de programmation

Conviron

Conviron GEN1000-GE Manuel utilisateur

Parr Instrument

Parr Instrument 4560 Mode d’emploi

IKA

IKA IC control Manuel utilisateur

Integra

Integra DOSE IT Manuel utilisateur

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manuel utilisateur

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manuel utilisateur

NI PXI-5422 Manuel

Collomix

Collomix Aqix Manuel utilisateur

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manuel utilisateur

Ocean Insight

Ocean Insight FLAME-NIR+ Manuel utilisateur

Parker

Parker ALIGN-MG-NA Manuel utilisateur

BD 644787 Manuel utilisateur

DENTAURUM

DENTAURUM Compact Megaplus Manuel utilisateur

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manuel utilisateur

VWR

VWR SAS Super IAQ Manuel utilisateur

illumina

illumina MiSeqDx Manuel utilisateur