Thermo Scientific A2-OK Mode d'emploi

Horizontal System

Model A2-OK

Operating and Maintenance Manual 7219011 Rev. 0

Visit us online to register your warranty

www.thermoscientific.com/warranty

Thermo Scientific

MANUAL NUMBER 7219011

0 -- 4/13/12 Transfer to Marietta (was 11/2002) ccs

REV ECR/ECN DATE DESCRIPTION By

Preface

Horizontal System i

Thermo Scientificii Horizontal System

Preface

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual

may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Warning To avoid the risk of personal shock, always disconnect the gel box from the power supply. Further, the

power supply must be equipped with a shut-down-on-disconnect circuit. Do not move the unit unless the power

source to the unit has been disconnected. s

Statement of Proper Use: Use this product only for its intended purpose as described in this manual. Do not use

this product if the power leads are damaged or if any of its surfaces are cracked.

Warning Running conditions for this unit should not exceed the name plate readings found on the lower buffer

chamber. s

This Owl System is designed to meet IEC 1010-1 safety standards (IEC 1010-1 is an internationally accepted

electrical safety standard for laboratory instruments).

Material in this manual is for information purposes only. The contents and the product it describes are subject to

change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this

manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to

the use of this manual.

Thermo Scientific Horizontal System iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this

symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be

damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment

falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market

after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic

Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher

Scientific has contracted with one or more recycling/disposal companies in each EU Member State

European Country, and this product should be disposed of or recycled through them. Further

information on Thermo’s compliance with this directive, the recyclers in your country and

information on Thermo products will be available at www.thermofisher.com.

4Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4Always dissipate extreme cold or heat and wear protective clothing.

4Always follow good hygiene practices.

4Each individual is responsible for his or her own safety.

Thermo Scientificiv Horizontal System

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct

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http://www.thermoscientific.com Internet Worldwide Web Home Page

service.led.marietta@thermofisher.com Tech Support Email Address

Certified Service Web Page

Thermo Fisher Scientific

401 Millcreek Road, Box 649

Marietta, OH 45750

Our staff can provide information on pricing and give you quotations. We can

take your order and provide delivery information on major equipment items or make

arrangements to have your local sales representative contact you. Our products are listed on the

Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation or

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or

provide you with on-site service. We can also provide you with a quotation on our Extended

Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your

applications. If you are experiencing technical problems, working together, we will help you

locate the problem and, chances are, correct it yourself...over the telephone without a service

call.

When more extensive service is necessary, we will assist you with direct factory trained

technicians or a qualified service organization for on-the-spot repair. If your service need is

covered by the warranty, we will arrange for the unit to be repaired at our expense and to your

satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you

Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by

telephone or fax. If you wish to write, our mailing address is:

International customers, please contact your local Thermo Scientific distributor.

Sales Support

Service Support

www.unitylabservices.com

Horizontal System vThermo Scientific

Table of Contents

General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Setting Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Preparing the Gel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Using the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Finishing Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Specifications and Recommended Running Conditions . . . . . . . . . . .4-1

Technical Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Combs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Reagent Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Care and Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Optional Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Section 1

Section 2

Section 3

Section 4

Section 5

Section 6

Section 8

Section 7

Horizontal System 1-1Thermo Scientific

Section 1 General Information

The Opossum Multiple Gel system is ideal for teaching labs or labs that

need to run multiple gels simultaneously. Up to six minigels (7cmW x

8cmL) can be run on this versatile system. Along with optional combs the

Opossum can screen up to 144 samples at once (120 with supplied

combs). EasyCast casting design allows the casting of gels without tape or

plugs. UV transmissible gel trays may be placed directly on the

transilluminator for photodocumentation.

Before starting, unpack the unit and inventory your order. If any parts are

missing, contact Technical Services immediately.

Reference the order or catalog number on your invoice and check the

corresponding parts list.

Table 1-1. Parts List

Buffer Chamber

Combs 10 well comb, Double Sided, 1.0/1.5mm Thick (12)

Super Safe Lid with attached power supply leads

Multiple Gel Caster (2)

Gel Tray Holder

UV Transmissible gel trays (6)

1-2 Horizontal System Thermo Scientific

Section 1

General Information

Figure 1-1. Exploded Parts Diagram

Table 1-1. Specifications

Gel size . . . . . . . . . . . . . . . . . . . . . . .7cmW x 8cmL

Running Buffer . . . . . . . . . . . . . . . . . .Volume 2.3L

Footprint . . . . . . . . .28cm W x 37.5cm L x 10cm H

Power Supply

Leads Super Safe Lid

UVT Gel

Tray (6)

Gel Tray

Holder

Buffer

Chamber

Bubble Level

Double Sided

Comb (12)

Multiple Gel

Caster (2)

Gasket (12)

1.5 Thick

1.0 Thick

Horizontal System 2-1Thermo Scientific

Section 2 Setting Up

1. For shipping and convenient storage, the gel tray is packaged inside the

unit with the gasketed end gates in

position upon arrival. Lift the casting

tray out of the buffer chamber.

2. To cast gels, place the gel tray(s) into

the external multiple gel caster. The

gasketed sides of the UVT gel tray

form a leak proof seal along the side

of the gel caster.

3. Prepare and pour warm (>60) agarose solution onto UVT gel tray.

Place comb(s) into slots provided on walls of UVT gel tray.

4. One the gel has solidified, place the UVT gel tray (s) into the gel tray

holder.

5. Level the casting chamber using the thumbscrews on each side of the

unit by slowly turning one thumbscrew at a time and lining up the

bubble in the level with the center circle. Place the gel tray holder in

the buffer chamber, add buffer, remove combs, load samples and run

the gel.

Figure 2-1. Casting Tray

Figure 2-2. Pour Agarose Figure 2-3. Place Combs

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