Thermo Electron BBD 6220 Manuel utilisateur

Operating instructions

Hot-Air Disinfectable Gassed Incubator

BBD 6220

Read this operating instructions and keep it near the equipment for reference purposes !

Valid: 03.2006 / 50 079 041 A

Postal adress Germany

Thermo Electron LED GmbH

Robert-Bosch-Straße 1

D - 63505 Langenselbold

Enquiries from Germany

Phone

Sale 0800 1 536376

Service 0800 1 112110

Fax

Sale/Service

0800 1 112114

E-Mail

info.labequipment.de@thermo.com

Enquiries from Europe, Middle East and Africa

Tel. + 49 (0) 6184 / 90-6940

Fax + 49 (0) 6184 / 90-7474

E-Mail

info.labequipment.de@thermo.com

Postal address USA

Thermo Electron Corporation

275 Aiken Road

Asheville, NC 28804

USA

Enquiries from North America

Phone +1 800-879 7767

Fax +1 828-658 0363

E-Mail

info.labequipment.de@thermo.com

Enquiries from Latin America

Phone +1 828-658 2711

Fax +1 828-645 9466

E-Mail

iinfo.labequipment.de@thermo.com

Enquiries from Asia Pacific

Phone +852-2711 3910

Fax +852-2711 3858

E-Mail

info.labequipment.de@thermo.com

No part of this publication may be reproduced or transmitted in any form or by any means, including pho-

tocopying and recording, without the written permission of Thermo Electron GmbH.

Various sections of this instruction manual may be copied only for in-house use by the equipment opera-

tor, e.g. to provide employees with instructions on accident prevention measures. These sections are

clearly marked in the list of contents.

Thermo Electron GmbH can accept no liability or responsibility for the marketability or the suitability of this

instruction manual for a certain purpose other than that specified under "Areas of application of equip-

ment".

Thermo Electron GmbH reserves the right to change the content of this instruction manual at any time

and without prior notice.

As regards foreign-language translations, the German version of this instruction manual is binding.

This edition of the instruction manual applies to the BBD 6220 unit specified on the front page.

Date of publication: 03.2006

Nominal charge

Safety with regard to the protection of persons, the environment and the material under process

largely depends on the conduct of the persons using the equipment.

This instruction manual is important for your safety as well as for the setup, installation, use and

maintenance of the equipment!

To avoid errors and any resulting damage, especially personal injuries, be sure to read this man-

ual carefully before using the equipment and follow all instructions.

2/43

50 079 041

1. GENERAL ................................................................................ 5/7

Explanation of icons in the instructions manual ..................................................... 5

Explanation of icons in the short instructions ....................................................... 6

Generalinstructions ............................................................................... 7

Equipmentlogbook ............................................................................... 7

Operatinginstructions ............................................................................. 7

2. AREASOFAPPLICATION ................................................................... 8

3. SAFETY INSTRUCTIONS . . .. . . . . . .. . . . . . . .. . . .. . . . . . .. . . . . . . .. . . . . . .. . . . . . .. . . . . . .. . . . . . .. . . 9

4. SETUPANDINSTALLATION ............................................................ 10-12

Transport ......................................................................................... 10

Placeofinstallation .............................................................................. 10

Stacking .......................................................................................... 10

Clearances ....................................................................................... 11

Supplyconnections ............................................................................... 11

Mainsconnection ................................................................................. 11

Gassupplyconnection ............................................................................ 12

RS232interface ................................................................................. 12

"Potential-free contact" connection ................................................................ 12

5. DESCRIPTIONOFTHEUNIT ............................................................ 13/14

Levellingtheshelves ............................................................................. 13

Functionalprinciple ............................................................................... 13

Heatingsystem ................................................................................... 13

Gassing .......................................................................................... 13

Humidification .................................................................................... 14

Doorswitch ....................................................................................... 14

Internalfittings .................................................................................... 14

Six-piece Gas-tight glass screen ................................................................. 14

Lockableoutsidedoor ............................................................................ 14

Disinfectionroutine ............................................................................... 14

"Potential-free contact" connection ................................................................ 14

6. OPERATE .............................................................................. 15-30

Switchpanel ...................................................................................... 15

Watersupply ..................................................................................... 16

Mainsswitch ..................................................................................... 16

Temperature protection device (temperature limit controller) ....................................... 18

Control and display panels .................................................................... 18-21

Disinfectionroutine ............................................................................ 21/22

auto-start routine / auto-zero routine ........................................................... 23/24

Error code enquiry ............................................................................ 23/25

Switchingfunctions ............................................................................... 27

Switchingfunction:AcousticalarmON/OFF ....................................................... 27

Switchingfunction:HumidificationON/OFF ........................................................ 28

Switching function: Manual zero calibration of CO2measuringsystem ............................. 29

Switchingfunction:Setpointlock-inON/OFF ...................................................... 30

Switching function: Door heater ON/OFF .......................................................... 31

LIST OF CONTENTS PAGE

50 079 041 3/45

7. START-UP .................................................................................... 32

8. OPERATION .................................................................................. 33

Guidelinesforuse ................................................................................... 33

Before putting the unit into operation ................................................................. 33

Duringoperation ..................................................................................... 33

Operationinterruptions .............................................................................. 33

9. SHUTDOWN ................................................................................. 34

10.MAINTENANCE ............................................................................ 35/36

Cleaning/Disinfection ................................................................................ 35

Testing ............................................................................................. 36

Equipmentlogbook ................................................................................. 36

Replacingofelectricalparts .......................................................................... 36

11. AUTHORIZED EQUIPMENT PARTS AND ACCESSORIES . . . . . .. . . . . . .. . . . . . .. . . . . . .. . . . . . .. . . . . 37

12. TECHNICAL SPECIFICATIONS . . . . . . . .. . . . . . . .. . . . . . .. . . . . . .. . . . . . .. . . . . . .. . . . . . .. . . . .. . . . . 38/39

13.MATERIALSUSED/DISPOSAL ............................................................... 40

14.GASCONSUMPTION ......................................................................... 41

15.PH-VALUEOFCULTUREMEDIA .............................................................. 42

Basic rules of sound microbiological engineering practice . . . . . . . . .. . . . . . .. . . .. . . . . . . .. . . . . 43/44

Generalrules ........................................................................................ 43

Handling of pathogenes .......................................................................... 43/44

Handling of human- and animal-pathogenic biological agents ......................................... 44

APPENDIX

Statement of conformity

Log Book

Explanation of confirmation of safety

Information request form regarding maintenance and servicing contract

Communication-protocol of RS 232-device

LIST OF CONTENTS PAGE

4/43

50 079 041

EXPLANATION OF ICONS IN THE INSTRUCTIONS MANUAL

In chapters of the instruction manual which deal with safety, this icon appears under

the title of the chapter. Displayed on the equipment, this icon denotes that special at-

tention must be paid to the information given in the instruction manual or accompany-

ing documents.

Marks information in the instruction manual for optimizing use of the equipment

"Water supply" vent/overflow

"Add/drain water" quick release coupling

aqua dest

IUnit ON

0Unit OFF

des

start/

stop "Disinfection mode" key-operated switch

"des" Marks information for higher surface temperature in the disinfection mode

Overtemperature protection, temperature limit controller

Selection of switching/interlocking functions

iError code enquiry / acknowledgment of "acoustic signal"

control "Change switching functions activated" display

auto-start Automatic startup of unit and calibration of measuring systems

auto-zero "auto-zero" calibration active display

des "Disinfection mode" display

Information on proper disposal / recovery of raw materials

1. GENERAL

50 079 041 5/45

EXPLANATION OF ICONS TO THE SHORT INSTRUCTIONS

ISwitch on unit (operate the main switch)

max.

min. aqua dest. Check the water level

°C

> + 2 °C

Set the temperature limit controller

Close unit doors

LRead the operating instructions

des

start/

stop

≥1 sec

Start "disinfection routine"

auto-start automatical "auto-start"

30 s

≥

Open unit doors for at least 30 seconds

&





&2

2







U



Set the desired setpoints

≥5 s &

start / stop

auto-start "activate" the auto-start

auto-start End of the auto-start, bring in the samples

> 30 s

Take out the samples, open unit doors for at least

30 seconds

180 °C

Flow chart of disinfection routine

1. GENERAL

6/43

50 079 041

General instructions

To avoid errors and causing damage, especially personal injuries, be sure to read this manual

carefully before using the equipment, and follow all instructions.

When setting up and operating the unit, be sure to comply with the instructions contained in this

manual as well as all applicable laws, regulations and directives in your country (FRG: ZH 1/119,

ZH 1/342, ZH 1/343, ZH 1/598).

The unit meets the following safety requirements

DIN 12880 Part 1/11.78

DIN VDE 0700 Part 1 (IEC 335-1; EN 60335-1/11.90)

EN 61010

When you have an enquiry, order replacement parts or file a complaint, please state the data on

the nameplate and, if applicable, the fault code.

Equipment log book

We advise you to keep an equipment log book.

Keep a record of inspections and testing, calibration work and any major work carried out on the

unit - such as maintenance, agents loaded, etc. - in this log book (refer to APPENDIX).

Operating instructions

The operator (employer) is expected to provide anyone who works on or with the equipment with

written instructions, based on this instruction manual, for the tasks to be performed. These in-

structions should be easy to understand and in the language of the persons operating the equip-

ment.

This also applies to disinfecting and cleaning the unit (also refer to Chapter 10: Maintenance).

1. GENERAL

50 079 041 7/45

The BBD 6220 gassed incubator is an item of laboratory equipment for cell and tissue cultures

and can be used to simulate the physiological environmental conditions of cells.

The unit is generally suitable for setup and operation in the following areas:

In laboratories for microbiological and biotechnological work.

Medical and microbiological laboratories to DIN 58956.

Central laboratories in clinics and hospitals.

Laboratories operating at safety levels L1, L2 and L3.

Only organisms which comply with the requirements for safety levels L1, L2 and L3 may be

handled in the unit.

The unit must be set up and operated in accordance with all applicable regulations in your

country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598)

It is not permitted to handle gases or agents whose vapours are combustible or can form a

hazardous, potentially explosive atmosphere when mixed with air.

Be sure to comply with the applicable regulations in your country.

(FRG: ZH 1/10)

2. AREAS OF APPLICATION

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50 079 041

In the case of biological incubators, biological safety with regard to the protection of

persons, its surroundings and the load is heavily dependent on the observance of the

applicable regulations by the persons using the equipment.

Even then, however, the possibility of hazards, especially health hazards, arising cannot be

ruled out.

The residual risk depends on the work performed in each individual case.

To avoid errors and causing damage, especially personal injuries, be sure to carefully read

this manual before putting the equipment into operation, and follow all instructions.

Safe and reliable operation of the unit can only be guaranteed if the necessary inspections,

maintenance and repair work are carried out by Heraeus Service Department personnel or

by personnel authorized by our company.

Gas is to be supplied to each unit by means of a pressure reducer with the inlet pressure

set to between 0.8 and max. 1 bar. This setting must not be changed for safety reasons

(FRG: ZH 1/119).

The place of installation must be thoroughly ventilated in order to expel the gases escaping

around the pressure relief valve to the outside.

To maintain the temperature protection function, the functioning of the overtemperature

protection device, temperatur limit controller must be checked at reasonable intervals.

Only original replacement parts authorized by the manufacturer are to be used.

Refer to the Appendix titled "BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEER-

ING PRACTICE".

3. SAFETY INSTRUCTIONS

50 079 041 9/45

When setting up, installing and operating the unit, be sure to comply with all applicable regulations

in your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598)

Transport

Handle the unit with care during transport. Do not lift up the unit by its door.

Refer to TECHNICAL SPECIFICATIONS for weights and dimensions.

Place of installation

The place of installation must be dry and draught-free.

The unit should not be placed or operated in recesses which cannot be ventilated.

To avoid or at least minimize deviations from the technical specifications, the tempera-

ture at the place of installation must be in the range +18°C to +30°C.

Set up the unit on a solid, non-flammable surface (laboratory tables, subframes).

Make sure that the unit is in a stable and perfectly upright position.

Do not cover or obstruct the fresh air or exhaust vents in the unit housing

(please observe the minimum clearances - see page 11).

CO2are admitted into the gassed incubator. CO2 is a health risk hazard. The place of installa-

tion must therefore be well-ventilated.

Gases discharged from the pressure relief valve must be safely expelled outside by means of the

ventilation systems.

It may be necessary to take special measures in order to ensure proper ventilation when several

units are installed in the same room.

For information on gas flow during operation, refer to the APPENDIX.

Stacking

The unit is not suitable for stacking when operated in the manner intended.

4. SETUP AND INSTALLATION

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50 079 041

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